[2024-01-24 11:06:04,660] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:06:04,662] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:06:04,662] [INFO] DQC Reference Directory: /var/lib/cwl/stg6f56a702-4fa0-47b6-841b-21e0715e208f/dqc_reference
[2024-01-24 11:06:07,010] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:06:07,012] [INFO] Task started: Prodigal
[2024-01-24 11:06:07,012] [INFO] Running command: gunzip -c /var/lib/cwl/stga7eab8b0-f1da-4b2a-867e-0c044dda4b51/GCF_004765785.1_ASM476578v1_genomic.fna.gz | prodigal -d GCF_004765785.1_ASM476578v1_genomic.fna/cds.fna -a GCF_004765785.1_ASM476578v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:06:27,311] [INFO] Task succeeded: Prodigal
[2024-01-24 11:06:27,312] [INFO] Task started: HMMsearch
[2024-01-24 11:06:27,312] [INFO] Running command: hmmsearch --tblout GCF_004765785.1_ASM476578v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6f56a702-4fa0-47b6-841b-21e0715e208f/dqc_reference/reference_markers.hmm GCF_004765785.1_ASM476578v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:06:27,565] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:06:27,566] [WARNING] Found 3/6 markers. [/var/lib/cwl/stga7eab8b0-f1da-4b2a-867e-0c044dda4b51/GCF_004765785.1_ASM476578v1_genomic.fna.gz]
[2024-01-24 11:06:27,602] [INFO] Query marker FASTA was written to GCF_004765785.1_ASM476578v1_genomic.fna/markers.fasta
[2024-01-24 11:06:27,602] [INFO] Task started: Blastn
[2024-01-24 11:06:27,602] [INFO] Running command: blastn -query GCF_004765785.1_ASM476578v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6f56a702-4fa0-47b6-841b-21e0715e208f/dqc_reference/reference_markers.fasta -out GCF_004765785.1_ASM476578v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:06:28,128] [INFO] Task succeeded: Blastn
[2024-01-24 11:06:28,132] [INFO] Selected 7 target genomes.
[2024-01-24 11:06:28,132] [INFO] Target genome list was writen to GCF_004765785.1_ASM476578v1_genomic.fna/target_genomes.txt
[2024-01-24 11:06:28,136] [INFO] Task started: fastANI
[2024-01-24 11:06:28,136] [INFO] Running command: fastANI --query /var/lib/cwl/stga7eab8b0-f1da-4b2a-867e-0c044dda4b51/GCF_004765785.1_ASM476578v1_genomic.fna.gz --refList GCF_004765785.1_ASM476578v1_genomic.fna/target_genomes.txt --output GCF_004765785.1_ASM476578v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:06:36,425] [INFO] Task succeeded: fastANI
[2024-01-24 11:06:36,426] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6f56a702-4fa0-47b6-841b-21e0715e208f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:06:36,426] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6f56a702-4fa0-47b6-841b-21e0715e208f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:06:36,437] [INFO] Found 7 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 11:06:36,437] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:06:36,438] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Halorussus ruber	strain=YC25	GCA_004765785.1	1126238	1126238	type	True	100.0	1420	1423	95	conclusive
Halorussus salinus	strain=YJ-37-H	GCA_004765815.2	1364935	1364935	type	True	85.0266	1063	1423	95	below_threshold
Halorussus pelagicus	strain=RC-68	GCA_004087835.1	2505977	2505977	type	True	84.278	890	1423	95	below_threshold
Halorussus rarus	strain=TBN4	GCA_003369835.1	660515	660515	type	True	83.0903	944	1423	95	below_threshold
Halorussus amylolyticus	strain=YC93	GCA_004143485.2	1126242	1126242	type	True	81.9597	789	1423	95	below_threshold
Halorussus halobius	strain=HD8-83	GCA_004765805.1	1710537	1710537	type	True	81.6545	806	1423	95	below_threshold
Halorussus marinus	strain=LYG-36	GCA_004765795.1	2505976	2505976	type	True	81.0603	738	1423	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:06:36,439] [INFO] DFAST Taxonomy check result was written to GCF_004765785.1_ASM476578v1_genomic.fna/tc_result.tsv
[2024-01-24 11:06:36,440] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:06:36,440] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:06:36,440] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6f56a702-4fa0-47b6-841b-21e0715e208f/dqc_reference/checkm_data
[2024-01-24 11:06:36,441] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:06:36,482] [INFO] Task started: CheckM
[2024-01-24 11:06:36,482] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_004765785.1_ASM476578v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_004765785.1_ASM476578v1_genomic.fna/checkm_input GCF_004765785.1_ASM476578v1_genomic.fna/checkm_result
[2024-01-24 11:07:31,066] [INFO] Task succeeded: CheckM
[2024-01-24 11:07:31,068] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:07:31,087] [INFO] ===== Completeness check finished =====
[2024-01-24 11:07:31,088] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:07:31,088] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_004765785.1_ASM476578v1_genomic.fna/markers.fasta)
[2024-01-24 11:07:31,089] [INFO] Task started: Blastn
[2024-01-24 11:07:31,089] [INFO] Running command: blastn -query GCF_004765785.1_ASM476578v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6f56a702-4fa0-47b6-841b-21e0715e208f/dqc_reference/reference_markers_gtdb.fasta -out GCF_004765785.1_ASM476578v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:07:31,608] [INFO] Task succeeded: Blastn
[2024-01-24 11:07:31,610] [INFO] Selected 7 target genomes.
[2024-01-24 11:07:31,610] [INFO] Target genome list was writen to GCF_004765785.1_ASM476578v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:07:31,616] [INFO] Task started: fastANI
[2024-01-24 11:07:31,616] [INFO] Running command: fastANI --query /var/lib/cwl/stga7eab8b0-f1da-4b2a-867e-0c044dda4b51/GCF_004765785.1_ASM476578v1_genomic.fna.gz --refList GCF_004765785.1_ASM476578v1_genomic.fna/target_genomes_gtdb.txt --output GCF_004765785.1_ASM476578v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:07:41,408] [INFO] Task succeeded: fastANI
[2024-01-24 11:07:41,420] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:07:41,421] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_004765785.1	s__Halorussus ruber	100.0	1420	1423	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Halorussus	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_004765815.2	s__Halorussus salinus	85.0165	1064	1423	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Halorussus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_005239435.1	s__Halorussus sp005239435	84.7027	1040	1423	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Halorussus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_010747475.1	s__Halorussus sp010747475	84.4123	993	1423	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Halorussus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004087835.1	s__Halorussus sp004087835	84.2617	891	1423	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Halorussus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003369835.1	s__Halorussus rarus	83.0676	948	1423	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Halorussus	95.0	100.00	100.00	1.00	1.00	2	-
GCF_004765805.1	s__Halorussus sp004765805	81.6469	809	1423	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Haladaptataceae;g__Halorussus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:07:41,422] [INFO] GTDB search result was written to GCF_004765785.1_ASM476578v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:07:41,422] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:07:41,425] [INFO] DFAST_QC result json was written to GCF_004765785.1_ASM476578v1_genomic.fna/dqc_result.json
[2024-01-24 11:07:41,425] [INFO] DFAST_QC completed!
[2024-01-24 11:07:41,425] [INFO] Total running time: 0h1m37s