[2024-01-24 12:34:20,637] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:34:20,640] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:34:20,640] [INFO] DQC Reference Directory: /var/lib/cwl/stgd8387d97-5142-48b6-b2b4-1745f8952d99/dqc_reference
[2024-01-24 12:34:21,868] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:34:21,868] [INFO] Task started: Prodigal
[2024-01-24 12:34:21,869] [INFO] Running command: gunzip -c /var/lib/cwl/stg78a274bd-35e8-4c8a-9240-9fe0396b4c5f/GCF_005217845.1_ASM521784v1_genomic.fna.gz | prodigal -d GCF_005217845.1_ASM521784v1_genomic.fna/cds.fna -a GCF_005217845.1_ASM521784v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:34:32,031] [INFO] Task succeeded: Prodigal
[2024-01-24 12:34:32,031] [INFO] Task started: HMMsearch
[2024-01-24 12:34:32,032] [INFO] Running command: hmmsearch --tblout GCF_005217845.1_ASM521784v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd8387d97-5142-48b6-b2b4-1745f8952d99/dqc_reference/reference_markers.hmm GCF_005217845.1_ASM521784v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:34:32,339] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:34:32,341] [INFO] Found 6/6 markers.
[2024-01-24 12:34:32,397] [INFO] Query marker FASTA was written to GCF_005217845.1_ASM521784v1_genomic.fna/markers.fasta
[2024-01-24 12:34:32,398] [INFO] Task started: Blastn
[2024-01-24 12:34:32,398] [INFO] Running command: blastn -query GCF_005217845.1_ASM521784v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd8387d97-5142-48b6-b2b4-1745f8952d99/dqc_reference/reference_markers.fasta -out GCF_005217845.1_ASM521784v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:34:32,986] [INFO] Task succeeded: Blastn
[2024-01-24 12:34:32,990] [INFO] Selected 14 target genomes.
[2024-01-24 12:34:32,990] [INFO] Target genome list was writen to GCF_005217845.1_ASM521784v1_genomic.fna/target_genomes.txt
[2024-01-24 12:34:32,997] [INFO] Task started: fastANI
[2024-01-24 12:34:32,997] [INFO] Running command: fastANI --query /var/lib/cwl/stg78a274bd-35e8-4c8a-9240-9fe0396b4c5f/GCF_005217845.1_ASM521784v1_genomic.fna.gz --refList GCF_005217845.1_ASM521784v1_genomic.fna/target_genomes.txt --output GCF_005217845.1_ASM521784v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:34:47,779] [INFO] Task succeeded: fastANI
[2024-01-24 12:34:47,780] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd8387d97-5142-48b6-b2b4-1745f8952d99/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:34:47,781] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd8387d97-5142-48b6-b2b4-1745f8952d99/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:34:47,798] [INFO] Found 14 fastANI hits (9 hits with ANI > threshold)
[2024-01-24 12:34:47,798] [INFO] The taxonomy check result is classified as 'inconclusive'.
[2024-01-24 12:34:47,798] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Lysinibacillus mangiferihumi	strain=CCTCC AB 2010389	GCA_005217845.1	1130819	1130819	type	True	100.0	1664	1669	95	inconclusive
Lysinibacillus mangiferihumi	strain=M-GX18	GCA_003049665.1	1130819	1130819	type	True	99.9751	1642	1669	95	inconclusive
Lysinibacillus tabacifolii	strain=KCTC 33042	GCA_005217585.1	1173107	1173107	suspected-type	True	96.8578	1297	1669	95	inconclusive
Lysinibacillus sphaericus	strain=NBRC 15095	GCA_006539805.1	1421	1421	suspected-type	True	96.8484	1273	1669	95	inconclusive
Lysinibacillus varians	strain=NBRC 109424	GCA_005217815.1	1145276	1145276	suspected-type	True	96.8462	1313	1669	95	inconclusive
Lysinibacillus varians	strain=GY32	GCA_000600105.1	1145276	1145276	suspected-type	True	96.8297	1323	1669	95	inconclusive
Lysinibacillus tabacifolii	strain=K3514	GCA_003049565.1	1173107	1173107	suspected-type	True	96.8077	1300	1669	95	inconclusive
Lysinibacillus sphaericus	strain=NRRL B-23268	GCA_005217555.1	1421	1421	suspected-type	True	96.7985	1280	1669	95	inconclusive
Lysinibacillus sphaericus	strain=NCTC10338	GCA_900445465.1	1421	1421	suspected-type	True	96.726	1323	1669	95	inconclusive
Lysinibacillus cavernae	strain=SYSU K30005	GCA_009724685.1	2666135	2666135	type	True	81.539	745	1669	95	below_threshold
Lysinibacillus contaminans	strain=DSM 25560	GCA_001278945.1	1293441	1293441	type	True	80.3379	600	1669	95	below_threshold
Paenisporosarcina cavernae	strain=K2R23-3	GCA_003595195.1	2320858	2320858	type	True	77.8567	83	1669	95	below_threshold
Bacillus mycoides	strain=DSM 2048	GCA_022630575.1	1405	1405	type	True	76.407	83	1669	95	below_threshold
Lederbergia wuyishanensis	strain=CGMCC 1.12709	GCA_022900255.1	1347903	1347903	type	True	75.5967	53	1669	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:34:47,799] [INFO] DFAST Taxonomy check result was written to GCF_005217845.1_ASM521784v1_genomic.fna/tc_result.tsv
[2024-01-24 12:34:47,801] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:34:47,801] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:34:47,801] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd8387d97-5142-48b6-b2b4-1745f8952d99/dqc_reference/checkm_data
[2024-01-24 12:34:47,802] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:34:47,851] [INFO] Task started: CheckM
[2024-01-24 12:34:47,851] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_005217845.1_ASM521784v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_005217845.1_ASM521784v1_genomic.fna/checkm_input GCF_005217845.1_ASM521784v1_genomic.fna/checkm_result
[2024-01-24 12:35:22,400] [INFO] Task succeeded: CheckM
[2024-01-24 12:35:22,401] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:35:22,423] [INFO] ===== Completeness check finished =====
[2024-01-24 12:35:22,424] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:35:22,424] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_005217845.1_ASM521784v1_genomic.fna/markers.fasta)
[2024-01-24 12:35:22,425] [INFO] Task started: Blastn
[2024-01-24 12:35:22,425] [INFO] Running command: blastn -query GCF_005217845.1_ASM521784v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd8387d97-5142-48b6-b2b4-1745f8952d99/dqc_reference/reference_markers_gtdb.fasta -out GCF_005217845.1_ASM521784v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:35:23,249] [INFO] Task succeeded: Blastn
[2024-01-24 12:35:23,253] [INFO] Selected 9 target genomes.
[2024-01-24 12:35:23,254] [INFO] Target genome list was writen to GCF_005217845.1_ASM521784v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:35:23,262] [INFO] Task started: fastANI
[2024-01-24 12:35:23,262] [INFO] Running command: fastANI --query /var/lib/cwl/stg78a274bd-35e8-4c8a-9240-9fe0396b4c5f/GCF_005217845.1_ASM521784v1_genomic.fna.gz --refList GCF_005217845.1_ASM521784v1_genomic.fna/target_genomes_gtdb.txt --output GCF_005217845.1_ASM521784v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:35:34,308] [INFO] Task succeeded: fastANI
[2024-01-24 12:35:34,318] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:35:34,319] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003049665.1	s__Lysinibacillus mangiferihumi	99.9751	1642	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	96.9114	99.98	99.98	0.98	0.98	2	conclusive
GCF_002982115.1	s__Lysinibacillus sphaericus	96.8399	1314	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	96.9114	99.42	98.91	0.95	0.90	10	-
GCF_001728805.1	s__Lysinibacillus sphaericus_D	95.9389	1269	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	96.1498	99.28	99.28	0.92	0.92	2	-
GCA_015999545.1	s__Lysinibacillus sp002340205	86.3396	1133	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	95.0	98.79	97.71	0.94	0.94	5	-
GCA_007362955.1	s__Lysinibacillus fusiformis_E	82.5633	866	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003049575.1	s__Lysinibacillus parviboronicapiens	82.4861	860	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	95.0	99.01	98.61	0.95	0.92	4	-
GCF_000392615.1	s__Lysinibacillus sphaericus_B	82.2727	883	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001708185.1	s__Lysinibacillus xylanilyticus_A	82.0085	828	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016638705.1	s__Lysinibacillus sp001278875	81.904	809	1669	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_A;f__Planococcaceae;g__Lysinibacillus	95.0	99.95	99.95	0.96	0.96	2	-
--------------------------------------------------------------------------------
[2024-01-24 12:35:34,320] [INFO] GTDB search result was written to GCF_005217845.1_ASM521784v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:35:34,322] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:35:34,326] [INFO] DFAST_QC result json was written to GCF_005217845.1_ASM521784v1_genomic.fna/dqc_result.json
[2024-01-24 12:35:34,326] [INFO] DFAST_QC completed!
[2024-01-24 12:35:34,326] [INFO] Total running time: 0h1m14s