[2024-01-24 13:49:41,080] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:49:41,089] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:49:41,090] [INFO] DQC Reference Directory: /var/lib/cwl/stg842acf9d-a170-4f89-808f-86d2aa071289/dqc_reference
[2024-01-24 13:49:42,502] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:49:42,503] [INFO] Task started: Prodigal
[2024-01-24 13:49:42,504] [INFO] Running command: gunzip -c /var/lib/cwl/stg287d49ca-ba77-4348-b45d-8ca62a42c600/GCF_005925345.1_ASM592534v1_genomic.fna.gz | prodigal -d GCF_005925345.1_ASM592534v1_genomic.fna/cds.fna -a GCF_005925345.1_ASM592534v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:50:02,827] [INFO] Task succeeded: Prodigal
[2024-01-24 13:50:02,827] [INFO] Task started: HMMsearch
[2024-01-24 13:50:02,828] [INFO] Running command: hmmsearch --tblout GCF_005925345.1_ASM592534v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg842acf9d-a170-4f89-808f-86d2aa071289/dqc_reference/reference_markers.hmm GCF_005925345.1_ASM592534v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:50:03,085] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:50:03,086] [INFO] Found 6/6 markers.
[2024-01-24 13:50:03,125] [INFO] Query marker FASTA was written to GCF_005925345.1_ASM592534v1_genomic.fna/markers.fasta
[2024-01-24 13:50:03,125] [INFO] Task started: Blastn
[2024-01-24 13:50:03,125] [INFO] Running command: blastn -query GCF_005925345.1_ASM592534v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg842acf9d-a170-4f89-808f-86d2aa071289/dqc_reference/reference_markers.fasta -out GCF_005925345.1_ASM592534v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:50:03,763] [INFO] Task succeeded: Blastn
[2024-01-24 13:50:03,767] [INFO] Selected 16 target genomes.
[2024-01-24 13:50:03,767] [INFO] Target genome list was writen to GCF_005925345.1_ASM592534v1_genomic.fna/target_genomes.txt
[2024-01-24 13:50:03,776] [INFO] Task started: fastANI
[2024-01-24 13:50:03,776] [INFO] Running command: fastANI --query /var/lib/cwl/stg287d49ca-ba77-4348-b45d-8ca62a42c600/GCF_005925345.1_ASM592534v1_genomic.fna.gz --refList GCF_005925345.1_ASM592534v1_genomic.fna/target_genomes.txt --output GCF_005925345.1_ASM592534v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:50:18,638] [INFO] Task succeeded: fastANI
[2024-01-24 13:50:18,639] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg842acf9d-a170-4f89-808f-86d2aa071289/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:50:18,639] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg842acf9d-a170-4f89-808f-86d2aa071289/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:50:18,650] [INFO] Found 12 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 13:50:18,650] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:50:18,650] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Pedobacter ureilyticus	strain=THG-T11	GCA_005925345.1	1393051	1393051	type	True	100.0	1631	1632	95	conclusive
Pedobacter helvus	strain=P-25	GCA_005925325.1	2563444	2563444	type	True	82.3271	931	1632	95	below_threshold
Pedobacter xixiisoli	strain=CGMCC 1.12803	GCA_900215335.1	1476464	1476464	type	True	81.413	877	1632	95	below_threshold
Pedobacter xixiisoli	strain=S27	GCA_005925365.1	1476464	1476464	type	True	81.4073	884	1632	95	below_threshold
Pedobacter nanyangensis	strain=Q-4	GCA_003313505.1	1562389	1562389	type	True	79.8727	615	1632	95	below_threshold
Pedobacter chitinilyticus	strain=CM134L-2	GCA_003313385.1	2233776	2233776	type	True	79.6496	660	1632	95	below_threshold
Pedobacter chitinilyticus	strain=CM134L-2	GCA_004025525.1	2233776	2233776	type	True	79.5997	660	1632	95	below_threshold
Pedobacter frigiditerrae	strain=RP-1-13	GCA_004331645.1	2530452	2530452	type	True	78.0754	306	1632	95	below_threshold
Pedobacter planticolens	strain=LMG 31464	GCA_014172375.1	2679964	2679964	type	True	77.7432	345	1632	95	below_threshold
Pedobacter frigidisoli	strain=RP-3-11	GCA_004331675.1	2530455	2530455	type	True	77.6497	203	1632	95	below_threshold
Pedobacter kyungheensis	strain=KACC 16221	GCA_000812965.1	1069985	1069985	type	True	77.5855	236	1632	95	below_threshold
Mucilaginibacter gilvus	strain=F01003	GCA_004054195.1	2305909	2305909	type	True	76.9944	54	1632	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:50:18,654] [INFO] DFAST Taxonomy check result was written to GCF_005925345.1_ASM592534v1_genomic.fna/tc_result.tsv
[2024-01-24 13:50:18,654] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:50:18,655] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:50:18,655] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg842acf9d-a170-4f89-808f-86d2aa071289/dqc_reference/checkm_data
[2024-01-24 13:50:18,656] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:50:18,707] [INFO] Task started: CheckM
[2024-01-24 13:50:18,708] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_005925345.1_ASM592534v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_005925345.1_ASM592534v1_genomic.fna/checkm_input GCF_005925345.1_ASM592534v1_genomic.fna/checkm_result
[2024-01-24 13:51:12,830] [INFO] Task succeeded: CheckM
[2024-01-24 13:51:12,831] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:51:12,849] [INFO] ===== Completeness check finished =====
[2024-01-24 13:51:12,849] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:51:12,849] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_005925345.1_ASM592534v1_genomic.fna/markers.fasta)
[2024-01-24 13:51:12,850] [INFO] Task started: Blastn
[2024-01-24 13:51:12,850] [INFO] Running command: blastn -query GCF_005925345.1_ASM592534v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg842acf9d-a170-4f89-808f-86d2aa071289/dqc_reference/reference_markers_gtdb.fasta -out GCF_005925345.1_ASM592534v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:51:13,709] [INFO] Task succeeded: Blastn
[2024-01-24 13:51:13,713] [INFO] Selected 8 target genomes.
[2024-01-24 13:51:13,713] [INFO] Target genome list was writen to GCF_005925345.1_ASM592534v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:51:13,724] [INFO] Task started: fastANI
[2024-01-24 13:51:13,724] [INFO] Running command: fastANI --query /var/lib/cwl/stg287d49ca-ba77-4348-b45d-8ca62a42c600/GCF_005925345.1_ASM592534v1_genomic.fna.gz --refList GCF_005925345.1_ASM592534v1_genomic.fna/target_genomes_gtdb.txt --output GCF_005925345.1_ASM592534v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:51:21,911] [INFO] Task succeeded: fastANI
[2024-01-24 13:51:21,919] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:51:21,919] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_005925345.1	s__Pedobacter ureilyticus	100.0	1631	1632	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Pedobacter	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_005925325.1	s__Pedobacter helvus	82.3258	931	1632	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Pedobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900215335.1	s__Pedobacter xixiisoli	81.4182	876	1632	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Pedobacter	95.0	100.00	100.00	1.00	1.00	2	-
GCA_002399615.1	s__Pedobacter sp002399615	80.4868	614	1632	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Pedobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003313505.1	s__Pedobacter nanyangensis	79.899	612	1632	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Pedobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003313385.1	s__Pedobacter chitinilyticus	79.6464	660	1632	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Pedobacter	95.0	97.94	95.89	0.93	0.87	3	-
GCF_003313335.1	s__Pedobacter zeaxanthinifaciens	79.3791	591	1632	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Pedobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014172375.1	s__Pedobacter planticolens	77.7315	347	1632	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Sphingobacteriales;f__Sphingobacteriaceae;g__Pedobacter	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:51:21,920] [INFO] GTDB search result was written to GCF_005925345.1_ASM592534v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:51:21,921] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:51:21,924] [INFO] DFAST_QC result json was written to GCF_005925345.1_ASM592534v1_genomic.fna/dqc_result.json
[2024-01-24 13:51:21,924] [INFO] DFAST_QC completed!
[2024-01-24 13:51:21,924] [INFO] Total running time: 0h1m41s