[2024-01-24 14:31:01,565] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:31:01,567] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:31:01,567] [INFO] DQC Reference Directory: /var/lib/cwl/stg5a0b2686-8b8d-4f40-94c6-87846a77ab86/dqc_reference
[2024-01-24 14:31:04,071] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:31:04,073] [INFO] Task started: Prodigal
[2024-01-24 14:31:04,073] [INFO] Running command: gunzip -c /var/lib/cwl/stg191138ca-d379-4c66-a525-1a44e166d5c9/GCF_006094355.1_ASM609435v1_genomic.fna.gz | prodigal -d GCF_006094355.1_ASM609435v1_genomic.fna/cds.fna -a GCF_006094355.1_ASM609435v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:31:09,438] [INFO] Task succeeded: Prodigal
[2024-01-24 14:31:09,438] [INFO] Task started: HMMsearch
[2024-01-24 14:31:09,438] [INFO] Running command: hmmsearch --tblout GCF_006094355.1_ASM609435v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg5a0b2686-8b8d-4f40-94c6-87846a77ab86/dqc_reference/reference_markers.hmm GCF_006094355.1_ASM609435v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:31:09,703] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:31:09,704] [INFO] Found 6/6 markers.
[2024-01-24 14:31:09,733] [INFO] Query marker FASTA was written to GCF_006094355.1_ASM609435v1_genomic.fna/markers.fasta
[2024-01-24 14:31:09,734] [INFO] Task started: Blastn
[2024-01-24 14:31:09,734] [INFO] Running command: blastn -query GCF_006094355.1_ASM609435v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg5a0b2686-8b8d-4f40-94c6-87846a77ab86/dqc_reference/reference_markers.fasta -out GCF_006094355.1_ASM609435v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:31:11,256] [INFO] Task succeeded: Blastn
[2024-01-24 14:31:11,259] [INFO] Selected 10 target genomes.
[2024-01-24 14:31:11,259] [INFO] Target genome list was writen to GCF_006094355.1_ASM609435v1_genomic.fna/target_genomes.txt
[2024-01-24 14:31:11,299] [INFO] Task started: fastANI
[2024-01-24 14:31:11,299] [INFO] Running command: fastANI --query /var/lib/cwl/stg191138ca-d379-4c66-a525-1a44e166d5c9/GCF_006094355.1_ASM609435v1_genomic.fna.gz --refList GCF_006094355.1_ASM609435v1_genomic.fna/target_genomes.txt --output GCF_006094355.1_ASM609435v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:31:18,221] [INFO] Task succeeded: fastANI
[2024-01-24 14:31:18,221] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg5a0b2686-8b8d-4f40-94c6-87846a77ab86/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:31:18,222] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg5a0b2686-8b8d-4f40-94c6-87846a77ab86/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:31:18,229] [INFO] Found 7 fastANI hits (5 hits with ANI > threshold)
[2024-01-24 14:31:18,230] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:31:18,230] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Staphylococcus saprophyticus	strain=ATCC 15305	GCA_006094355.1	29385	29385	type	True	100.0	862	864	95	conclusive
Staphylococcus saprophyticus	strain=NCTC7292	GCA_900458885.1	29385	29385	type	True	99.9973	860	864	95	conclusive
Staphylococcus saprophyticus	strain=ATCC 15305	GCA_000010125.1	29385	29385	type	True	99.9946	850	864	95	conclusive
Staphylococcus saprophyticus	strain=ATCC 15305	GCA_001997165.1	29385	29385	type	True	99.9629	838	864	95	conclusive
Staphylococcus saprophyticus	strain=NCTC 7292	GCA_002902025.2	29385	29385	type	True	99.9147	799	864	95	conclusive
Staphylococcus shinii	strain=K22-5M	GCA_017583065.1	2912228	2912228	type	True	82.289	611	864	95	below_threshold
Staphylococcus capitis subsp. capitis	strain=DSM 20326	GCA_025272975.1	72758	29388	type	True	79.2773	283	864	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:31:18,232] [INFO] DFAST Taxonomy check result was written to GCF_006094355.1_ASM609435v1_genomic.fna/tc_result.tsv
[2024-01-24 14:31:18,232] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:31:18,232] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:31:18,233] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg5a0b2686-8b8d-4f40-94c6-87846a77ab86/dqc_reference/checkm_data
[2024-01-24 14:31:18,234] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:31:18,265] [INFO] Task started: CheckM
[2024-01-24 14:31:18,266] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_006094355.1_ASM609435v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_006094355.1_ASM609435v1_genomic.fna/checkm_input GCF_006094355.1_ASM609435v1_genomic.fna/checkm_result
[2024-01-24 14:31:41,772] [INFO] Task succeeded: CheckM
[2024-01-24 14:31:41,774] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:31:41,792] [INFO] ===== Completeness check finished =====
[2024-01-24 14:31:41,792] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:31:41,792] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_006094355.1_ASM609435v1_genomic.fna/markers.fasta)
[2024-01-24 14:31:41,793] [INFO] Task started: Blastn
[2024-01-24 14:31:41,793] [INFO] Running command: blastn -query GCF_006094355.1_ASM609435v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg5a0b2686-8b8d-4f40-94c6-87846a77ab86/dqc_reference/reference_markers_gtdb.fasta -out GCF_006094355.1_ASM609435v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:31:42,605] [INFO] Task succeeded: Blastn
[2024-01-24 14:31:42,608] [INFO] Selected 10 target genomes.
[2024-01-24 14:31:42,608] [INFO] Target genome list was writen to GCF_006094355.1_ASM609435v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:31:42,622] [INFO] Task started: fastANI
[2024-01-24 14:31:42,622] [INFO] Running command: fastANI --query /var/lib/cwl/stg191138ca-d379-4c66-a525-1a44e166d5c9/GCF_006094355.1_ASM609435v1_genomic.fna.gz --refList GCF_006094355.1_ASM609435v1_genomic.fna/target_genomes_gtdb.txt --output GCF_006094355.1_ASM609435v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:31:49,896] [INFO] Task succeeded: fastANI
[2024-01-24 14:31:49,912] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:31:49,912] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000010125.1	s__Staphylococcus saprophyticus	99.9947	850	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.41	98.75	0.94	0.91	167	conclusive
GCF_013391405.1	s__Staphylococcus sp013391405	89.8407	739	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002614725.1	s__Staphylococcus edaphicus	85.4644	691	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016774515.1	s__Staphylococcus xylosus_A	82.5511	621	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.34	99.08	0.95	0.91	11	-
GCF_900097965.1	s__Staphylococcus caeli	82.2934	571	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.99	99.99	1.00	1.00	2	-
GCF_003697915.1	s__Staphylococcus pseudoxylosus	82.17	589	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.50	97.12	0.92	0.87	9	-
GCF_002732165.1	s__Staphylococcus xylosus	82.1075	579	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.41	97.01	0.95	0.90	48	-
GCF_000338275.1	s__Staphylococcus xylosus_B	81.9119	618	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.09	97.23	0.94	0.90	8	-
GCF_900458565.1	s__Staphylococcus equorum	81.8452	567	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.19	97.35	0.94	0.89	49	-
GCF_001006765.1	s__Staphylococcus succinus	81.1354	506	864	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	97.99	95.53	0.94	0.89	24	-
--------------------------------------------------------------------------------
[2024-01-24 14:31:49,914] [INFO] GTDB search result was written to GCF_006094355.1_ASM609435v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:31:49,914] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:31:49,917] [INFO] DFAST_QC result json was written to GCF_006094355.1_ASM609435v1_genomic.fna/dqc_result.json
[2024-01-24 14:31:49,917] [INFO] DFAST_QC completed!
[2024-01-24 14:31:49,917] [INFO] Total running time: 0h0m48s