[2024-01-24 14:12:46,576] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:12:46,578] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:12:46,578] [INFO] DQC Reference Directory: /var/lib/cwl/stg9dd8415f-dca5-467f-88d8-701304f2d1e0/dqc_reference
[2024-01-24 14:12:47,877] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:12:47,878] [INFO] Task started: Prodigal
[2024-01-24 14:12:47,879] [INFO] Running command: gunzip -c /var/lib/cwl/stgda64ad3a-14d9-42c0-a92b-93d33fa828d6/GCF_006385365.1_ASM638536v1_genomic.fna.gz | prodigal -d GCF_006385365.1_ASM638536v1_genomic.fna/cds.fna -a GCF_006385365.1_ASM638536v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:13:05,188] [INFO] Task succeeded: Prodigal
[2024-01-24 14:13:05,188] [INFO] Task started: HMMsearch
[2024-01-24 14:13:05,189] [INFO] Running command: hmmsearch --tblout GCF_006385365.1_ASM638536v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9dd8415f-dca5-467f-88d8-701304f2d1e0/dqc_reference/reference_markers.hmm GCF_006385365.1_ASM638536v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:13:05,451] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:13:05,453] [INFO] Found 6/6 markers.
[2024-01-24 14:13:05,486] [INFO] Query marker FASTA was written to GCF_006385365.1_ASM638536v1_genomic.fna/markers.fasta
[2024-01-24 14:13:05,487] [INFO] Task started: Blastn
[2024-01-24 14:13:05,487] [INFO] Running command: blastn -query GCF_006385365.1_ASM638536v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9dd8415f-dca5-467f-88d8-701304f2d1e0/dqc_reference/reference_markers.fasta -out GCF_006385365.1_ASM638536v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:13:06,098] [INFO] Task succeeded: Blastn
[2024-01-24 14:13:06,102] [INFO] Selected 23 target genomes.
[2024-01-24 14:13:06,103] [INFO] Target genome list was writen to GCF_006385365.1_ASM638536v1_genomic.fna/target_genomes.txt
[2024-01-24 14:13:06,114] [INFO] Task started: fastANI
[2024-01-24 14:13:06,114] [INFO] Running command: fastANI --query /var/lib/cwl/stgda64ad3a-14d9-42c0-a92b-93d33fa828d6/GCF_006385365.1_ASM638536v1_genomic.fna.gz --refList GCF_006385365.1_ASM638536v1_genomic.fna/target_genomes.txt --output GCF_006385365.1_ASM638536v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:13:19,642] [INFO] Task succeeded: fastANI
[2024-01-24 14:13:19,643] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9dd8415f-dca5-467f-88d8-701304f2d1e0/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:13:19,643] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9dd8415f-dca5-467f-88d8-701304f2d1e0/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:13:19,652] [INFO] Found 7 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 14:13:19,652] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:13:19,652] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Tannerella forsythia	strain=ATCC 43037	GCA_001006485.1	28112	28112	type	True	99.9649	1049	1082	95	conclusive
Parabacteroides distasonis	strain=FDAARGOS_1565	GCA_020735945.1	823	823	type	True	76.8523	50	1082	95	below_threshold
Parabacteroides distasonis	strain=NCTC11152	GCA_900445525.1	823	823	type	True	76.8277	51	1082	95	below_threshold
Parabacteroides merdae	strain=NCTC13052	GCA_900445495.1	46503	46503	type	True	76.7779	70	1082	95	below_threshold
Parabacteroides merdae	strain=ATCC 43184	GCA_000154105.1	46503	46503	type	True	76.6539	68	1082	95	below_threshold
Parabacteroides merdae	strain=ATCC 43184	GCA_025151215.1	46503	46503	type	True	76.6395	69	1082	95	below_threshold
Parabacteroides goldsteinii	strain=DSM 19448	GCA_000969835.1	328812	328812	type	True	76.4804	63	1082	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:13:19,654] [INFO] DFAST Taxonomy check result was written to GCF_006385365.1_ASM638536v1_genomic.fna/tc_result.tsv
[2024-01-24 14:13:19,655] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:13:19,655] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:13:19,655] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9dd8415f-dca5-467f-88d8-701304f2d1e0/dqc_reference/checkm_data
[2024-01-24 14:13:19,656] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:13:19,693] [INFO] Task started: CheckM
[2024-01-24 14:13:19,693] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_006385365.1_ASM638536v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_006385365.1_ASM638536v1_genomic.fna/checkm_input GCF_006385365.1_ASM638536v1_genomic.fna/checkm_result
[2024-01-24 14:14:11,638] [INFO] Task succeeded: CheckM
[2024-01-24 14:14:11,639] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:14:11,660] [INFO] ===== Completeness check finished =====
[2024-01-24 14:14:11,661] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:14:11,661] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_006385365.1_ASM638536v1_genomic.fna/markers.fasta)
[2024-01-24 14:14:11,661] [INFO] Task started: Blastn
[2024-01-24 14:14:11,662] [INFO] Running command: blastn -query GCF_006385365.1_ASM638536v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9dd8415f-dca5-467f-88d8-701304f2d1e0/dqc_reference/reference_markers_gtdb.fasta -out GCF_006385365.1_ASM638536v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:14:12,514] [INFO] Task succeeded: Blastn
[2024-01-24 14:14:12,519] [INFO] Selected 23 target genomes.
[2024-01-24 14:14:12,519] [INFO] Target genome list was writen to GCF_006385365.1_ASM638536v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:14:12,542] [INFO] Task started: fastANI
[2024-01-24 14:14:12,543] [INFO] Running command: fastANI --query /var/lib/cwl/stgda64ad3a-14d9-42c0-a92b-93d33fa828d6/GCF_006385365.1_ASM638536v1_genomic.fna.gz --refList GCF_006385365.1_ASM638536v1_genomic.fna/target_genomes_gtdb.txt --output GCF_006385365.1_ASM638536v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:14:27,272] [INFO] Task succeeded: fastANI
[2024-01-24 14:14:27,285] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:14:27,286] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001006485.1	s__Tannerella forsythia	99.9649	1049	1082	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Tannerella	95.0	98.78	98.50	0.94	0.91	12	conclusive
GCF_003860135.1	s__Tannerella forsythia_A	88.2848	756	1082	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Tannerella	95.0	97.92	97.92	0.85	0.85	2	-
GCF_000154105.1	s__Parabacteroides merdae	76.6721	67	1082	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	98.97	97.44	0.89	0.77	85	-
GCF_004793765.1	s__Parabacteroides distasonis_A	76.5527	50	1082	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	99.06	98.88	0.88	0.86	4	-
GCF_900162725.1	s__Marseille-P3160 sp900162725	76.4401	51	1082	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Marseille-P3160	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014287585.1	s__Parabacteroides sp014287585	76.3876	71	1082	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	99.33	98.97	0.95	0.91	4	-
GCA_017937385.1	s__Parabacteroides sp017937385	75.9252	62	1082	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900552465.1	s__Parabacteroides sp900552465	75.6759	56	1082	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	99.92	99.92	0.93	0.93	2	-
--------------------------------------------------------------------------------
[2024-01-24 14:14:27,288] [INFO] GTDB search result was written to GCF_006385365.1_ASM638536v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:14:27,289] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:14:27,291] [INFO] DFAST_QC result json was written to GCF_006385365.1_ASM638536v1_genomic.fna/dqc_result.json
[2024-01-24 14:14:27,291] [INFO] DFAST_QC completed!
[2024-01-24 14:14:27,291] [INFO] Total running time: 0h1m41s