[2024-01-24 14:05:59,954] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:05:59,956] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:05:59,956] [INFO] DQC Reference Directory: /var/lib/cwl/stg1106c5ad-5893-4503-bd11-ae15c28888eb/dqc_reference
[2024-01-24 14:06:01,217] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:06:01,218] [INFO] Task started: Prodigal
[2024-01-24 14:06:01,218] [INFO] Running command: gunzip -c /var/lib/cwl/stgeaeb9295-fe1d-47bc-bc8a-3ffa07dd003d/GCF_006539405.1_ASM653940v1_genomic.fna.gz | prodigal -d GCF_006539405.1_ASM653940v1_genomic.fna/cds.fna -a GCF_006539405.1_ASM653940v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:06:05,416] [INFO] Task succeeded: Prodigal
[2024-01-24 14:06:05,417] [INFO] Task started: HMMsearch
[2024-01-24 14:06:05,417] [INFO] Running command: hmmsearch --tblout GCF_006539405.1_ASM653940v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1106c5ad-5893-4503-bd11-ae15c28888eb/dqc_reference/reference_markers.hmm GCF_006539405.1_ASM653940v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:06:05,670] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:06:05,672] [INFO] Found 6/6 markers.
[2024-01-24 14:06:05,692] [INFO] Query marker FASTA was written to GCF_006539405.1_ASM653940v1_genomic.fna/markers.fasta
[2024-01-24 14:06:05,692] [INFO] Task started: Blastn
[2024-01-24 14:06:05,692] [INFO] Running command: blastn -query GCF_006539405.1_ASM653940v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg1106c5ad-5893-4503-bd11-ae15c28888eb/dqc_reference/reference_markers.fasta -out GCF_006539405.1_ASM653940v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:06:06,275] [INFO] Task succeeded: Blastn
[2024-01-24 14:06:06,278] [INFO] Selected 13 target genomes.
[2024-01-24 14:06:06,279] [INFO] Target genome list was writen to GCF_006539405.1_ASM653940v1_genomic.fna/target_genomes.txt
[2024-01-24 14:06:06,285] [INFO] Task started: fastANI
[2024-01-24 14:06:06,285] [INFO] Running command: fastANI --query /var/lib/cwl/stgeaeb9295-fe1d-47bc-bc8a-3ffa07dd003d/GCF_006539405.1_ASM653940v1_genomic.fna.gz --refList GCF_006539405.1_ASM653940v1_genomic.fna/target_genomes.txt --output GCF_006539405.1_ASM653940v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:06:11,398] [INFO] Task succeeded: fastANI
[2024-01-24 14:06:11,398] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1106c5ad-5893-4503-bd11-ae15c28888eb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:06:11,399] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1106c5ad-5893-4503-bd11-ae15c28888eb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:06:11,416] [INFO] Found 12 fastANI hits (11 hits with ANI > threshold)
[2024-01-24 14:06:11,417] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:06:11,417] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Lactobacillus delbrueckii subsp. bulgaricus	strain=NBRC 13953	GCA_006539405.1	1585	1584	type	True	99.9999	532	535	95	conclusive
Lactobacillus delbrueckii subsp. bulgaricus	strain=ATCC 11842	GCA_000056065.1	1585	1584	type	True	99.9957	534	535	95	conclusive
Lactobacillus delbrueckii subsp. bulgaricus	strain=DSM 20081	GCA_001437195.1	1585	1584	type	True	99.9771	532	535	95	conclusive
Lactobacillus delbrueckii subsp. sunkii	strain=JCM 17838	GCA_001190005.1	1050107	1584	suspected-type	True	97.48	481	535	95	conclusive
Lactobacillus delbrueckii subsp. sunkii	strain=JCM 17838	GCA_001888965.1	1050107	1584	suspected-type	True	97.4224	492	535	95	conclusive
Lactobacillus delbrueckii subsp. jakobsenii	strain=DSM 26046	GCA_004354615.1	1537158	1584	suspected-type	True	97.1989	461	535	95	conclusive
Lactobacillus delbrueckii subsp. delbrueckii	strain=DSM 20074	GCA_001433875.1	83684	1584	suspected-type	True	97.1163	436	535	95	conclusive
Lactobacillus delbrueckii subsp. indicus	strain=JCM 15610	GCA_001189855.1	249265	1584	type	True	97.062	486	535	95	conclusive
Lactobacillus delbrueckii subsp. jakobsenii	strain=DSM 26046	GCA_001888925.1	1537158	1584	suspected-type	True	97.0451	472	535	95	conclusive
Lactobacillus delbrueckii subsp. indicus	strain=DSM 15996	GCA_001435795.1	249265	1584	type	True	97.0392	479	535	95	conclusive
Lactobacillus delbrueckii subsp. indicus	strain=JCM 15610	GCA_001908415.1	249265	1584	type	True	97.019	494	535	95	conclusive
Lactobacillus equicursoris	strain=JCM 14600	GCA_001311765.1	420645	420645	type	True	82.311	316	535	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:06:11,419] [INFO] DFAST Taxonomy check result was written to GCF_006539405.1_ASM653940v1_genomic.fna/tc_result.tsv
[2024-01-24 14:06:11,419] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:06:11,420] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:06:11,420] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1106c5ad-5893-4503-bd11-ae15c28888eb/dqc_reference/checkm_data
[2024-01-24 14:06:11,422] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:06:11,448] [INFO] Task started: CheckM
[2024-01-24 14:06:11,448] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_006539405.1_ASM653940v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_006539405.1_ASM653940v1_genomic.fna/checkm_input GCF_006539405.1_ASM653940v1_genomic.fna/checkm_result
[2024-01-24 14:06:31,970] [INFO] Task succeeded: CheckM
[2024-01-24 14:06:31,972] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:06:31,991] [INFO] ===== Completeness check finished =====
[2024-01-24 14:06:31,991] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:06:31,992] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_006539405.1_ASM653940v1_genomic.fna/markers.fasta)
[2024-01-24 14:06:31,992] [INFO] Task started: Blastn
[2024-01-24 14:06:31,992] [INFO] Running command: blastn -query GCF_006539405.1_ASM653940v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg1106c5ad-5893-4503-bd11-ae15c28888eb/dqc_reference/reference_markers_gtdb.fasta -out GCF_006539405.1_ASM653940v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:06:32,808] [INFO] Task succeeded: Blastn
[2024-01-24 14:06:32,812] [INFO] Selected 9 target genomes.
[2024-01-24 14:06:32,812] [INFO] Target genome list was writen to GCF_006539405.1_ASM653940v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:06:32,826] [INFO] Task started: fastANI
[2024-01-24 14:06:32,826] [INFO] Running command: fastANI --query /var/lib/cwl/stgeaeb9295-fe1d-47bc-bc8a-3ffa07dd003d/GCF_006539405.1_ASM653940v1_genomic.fna.gz --refList GCF_006539405.1_ASM653940v1_genomic.fna/target_genomes_gtdb.txt --output GCF_006539405.1_ASM653940v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:06:35,993] [INFO] Task succeeded: fastANI
[2024-01-24 14:06:36,001] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:06:36,001] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001433875.1	s__Lactobacillus delbrueckii	97.1163	436	535	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	97.56	96.59	0.89	0.81	103	conclusive
GCA_004556255.1	s__Lactobacillus delbrueckii_B	94.5454	323	535	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016587775.1	s__Lactobacillus nasalidis	86.8069	325	535	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	99.92	99.91	0.92	0.89	3	-
GCF_009695985.1	s__Lactobacillus porci	82.2328	384	535	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	97.72	97.20	0.91	0.90	4	-
GCF_001434815.1	s__Lactobacillus equicursoris	82.2059	319	535	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	98.84	97.67	0.88	0.82	5	-
GCF_000615445.1	s__Lactobacillus hamsteri	78.0442	53	535	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	99.97	99.97	0.99	0.99	2	-
GCA_905214545.1	s__Lactobacillus sp905214545	77.1832	101	535	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 14:06:36,003] [INFO] GTDB search result was written to GCF_006539405.1_ASM653940v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:06:36,004] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:06:36,007] [INFO] DFAST_QC result json was written to GCF_006539405.1_ASM653940v1_genomic.fna/dqc_result.json
[2024-01-24 14:06:36,007] [INFO] DFAST_QC completed!
[2024-01-24 14:06:36,007] [INFO] Total running time: 0h0m36s