[2024-01-24 15:03:29,541] [INFO] DFAST_QC pipeline started.
[2024-01-24 15:03:29,542] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 15:03:29,543] [INFO] DQC Reference Directory: /var/lib/cwl/stg20d041d8-8f35-4906-9a49-d46b58790cdc/dqc_reference
[2024-01-24 15:03:30,768] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 15:03:30,769] [INFO] Task started: Prodigal
[2024-01-24 15:03:30,769] [INFO] Running command: gunzip -c /var/lib/cwl/stg46c3c2e2-59b2-4ebb-acde-ec0c6ff53a1b/GCF_007992795.1_ASM799279v1_genomic.fna.gz | prodigal -d GCF_007992795.1_ASM799279v1_genomic.fna/cds.fna -a GCF_007992795.1_ASM799279v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 15:03:34,876] [INFO] Task succeeded: Prodigal
[2024-01-24 15:03:34,876] [INFO] Task started: HMMsearch
[2024-01-24 15:03:34,877] [INFO] Running command: hmmsearch --tblout GCF_007992795.1_ASM799279v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg20d041d8-8f35-4906-9a49-d46b58790cdc/dqc_reference/reference_markers.hmm GCF_007992795.1_ASM799279v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 15:03:35,171] [INFO] Task succeeded: HMMsearch
[2024-01-24 15:03:35,173] [INFO] Found 6/6 markers.
[2024-01-24 15:03:35,201] [INFO] Query marker FASTA was written to GCF_007992795.1_ASM799279v1_genomic.fna/markers.fasta
[2024-01-24 15:03:35,201] [INFO] Task started: Blastn
[2024-01-24 15:03:35,202] [INFO] Running command: blastn -query GCF_007992795.1_ASM799279v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg20d041d8-8f35-4906-9a49-d46b58790cdc/dqc_reference/reference_markers.fasta -out GCF_007992795.1_ASM799279v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 15:03:35,864] [INFO] Task succeeded: Blastn
[2024-01-24 15:03:35,868] [INFO] Selected 10 target genomes.
[2024-01-24 15:03:35,869] [INFO] Target genome list was writen to GCF_007992795.1_ASM799279v1_genomic.fna/target_genomes.txt
[2024-01-24 15:03:35,875] [INFO] Task started: fastANI
[2024-01-24 15:03:35,875] [INFO] Running command: fastANI --query /var/lib/cwl/stg46c3c2e2-59b2-4ebb-acde-ec0c6ff53a1b/GCF_007992795.1_ASM799279v1_genomic.fna.gz --refList GCF_007992795.1_ASM799279v1_genomic.fna/target_genomes.txt --output GCF_007992795.1_ASM799279v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 15:03:41,972] [INFO] Task succeeded: fastANI
[2024-01-24 15:03:41,973] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg20d041d8-8f35-4906-9a49-d46b58790cdc/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 15:03:41,974] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg20d041d8-8f35-4906-9a49-d46b58790cdc/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 15:03:41,990] [INFO] Found 8 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 15:03:41,990] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 15:03:41,991] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Staphylococcus haemolyticus	strain=NCTC11042	GCA_900458595.1	1283	1283	type	True	99.9943	771	772	95	conclusive
Staphylococcus haemolyticus	strain=ATCC 29970	GCA_006094395.1	1283	1283	type	True	99.9932	771	772	95	conclusive
Staphylococcus haemolyticus	strain=NCTC 11042	GCA_002901805.1	1283	1283	type	True	99.9854	771	772	95	conclusive
Staphylococcus borealis	strain=51-48	GCA_013345165.1	2742203	2742203	type	True	88.1008	714	772	95	below_threshold
Staphylococcus borealis	strain=51-48	GCA_001224225.1	2742203	2742203	type	True	88.099	713	772	95	below_threshold
Staphylococcus taiwanensis	strain=NTUH-S172	GCA_020544305.1	2750012	2750012	type	True	83.6672	612	772	95	below_threshold
Staphylococcus aureus	strain=PartF-Saureus-RM8376	GCA_022869625.1	1280	1280	type	True	79.2482	292	772	95	below_threshold
Staphylococcus aureus	strain=NCTC 08532	GCA_002902205.1	1280	1280	type	True	79.1964	290	772	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 15:03:41,993] [INFO] DFAST Taxonomy check result was written to GCF_007992795.1_ASM799279v1_genomic.fna/tc_result.tsv
[2024-01-24 15:03:41,994] [INFO] ===== Taxonomy check completed =====
[2024-01-24 15:03:41,994] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 15:03:41,994] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg20d041d8-8f35-4906-9a49-d46b58790cdc/dqc_reference/checkm_data
[2024-01-24 15:03:41,996] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 15:03:42,023] [INFO] Task started: CheckM
[2024-01-24 15:03:42,023] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_007992795.1_ASM799279v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_007992795.1_ASM799279v1_genomic.fna/checkm_input GCF_007992795.1_ASM799279v1_genomic.fna/checkm_result
[2024-01-24 15:04:02,710] [INFO] Task succeeded: CheckM
[2024-01-24 15:04:02,711] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 15:04:02,733] [INFO] ===== Completeness check finished =====
[2024-01-24 15:04:02,734] [INFO] ===== Start GTDB Search =====
[2024-01-24 15:04:02,734] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_007992795.1_ASM799279v1_genomic.fna/markers.fasta)
[2024-01-24 15:04:02,734] [INFO] Task started: Blastn
[2024-01-24 15:04:02,735] [INFO] Running command: blastn -query GCF_007992795.1_ASM799279v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg20d041d8-8f35-4906-9a49-d46b58790cdc/dqc_reference/reference_markers_gtdb.fasta -out GCF_007992795.1_ASM799279v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 15:04:03,587] [INFO] Task succeeded: Blastn
[2024-01-24 15:04:03,590] [INFO] Selected 7 target genomes.
[2024-01-24 15:04:03,590] [INFO] Target genome list was writen to GCF_007992795.1_ASM799279v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 15:04:03,597] [INFO] Task started: fastANI
[2024-01-24 15:04:03,597] [INFO] Running command: fastANI --query /var/lib/cwl/stg46c3c2e2-59b2-4ebb-acde-ec0c6ff53a1b/GCF_007992795.1_ASM799279v1_genomic.fna.gz --refList GCF_007992795.1_ASM799279v1_genomic.fna/target_genomes_gtdb.txt --output GCF_007992795.1_ASM799279v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 15:04:07,857] [INFO] Task succeeded: fastANI
[2024-01-24 15:04:07,869] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 15:04:07,869] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_006094395.1	s__Staphylococcus haemolyticus	99.9932	771	772	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.59	96.62	0.93	0.87	385	conclusive
GCF_001224225.1	s__Staphylococcus borealis	88.099	713	772	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.37	97.44	0.93	0.89	22	-
GCF_004785665.1	s__Staphylococcus pragensis	82.0665	546	772	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.69	99.09	0.98	0.94	4	-
GCF_002902565.1	s__Staphylococcus petrasii	82.056	530	772	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.66	97.62	0.95	0.85	8	-
GCA_002902575.1	s__Staphylococcus croceilyticus	82.0331	525	772	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.95	99.95	1.00	1.00	2	-
GCF_003035445.1	s__Staphylococcus devriesei_A	81.8134	532	772	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	97.92	97.92	0.95	0.95	2	-
GCF_002902625.1	s__Staphylococcus devriesei	81.3306	505	772	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.59	98.61	0.94	0.91	9	-
--------------------------------------------------------------------------------
[2024-01-24 15:04:07,871] [INFO] GTDB search result was written to GCF_007992795.1_ASM799279v1_genomic.fna/result_gtdb.tsv
[2024-01-24 15:04:07,872] [INFO] ===== GTDB Search completed =====
[2024-01-24 15:04:07,875] [INFO] DFAST_QC result json was written to GCF_007992795.1_ASM799279v1_genomic.fna/dqc_result.json
[2024-01-24 15:04:07,876] [INFO] DFAST_QC completed!
[2024-01-24 15:04:07,876] [INFO] Total running time: 0h0m38s