[2024-01-24 13:35:27,521] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:35:27,523] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:35:27,523] [INFO] DQC Reference Directory: /var/lib/cwl/stg24590a6f-b3e0-4218-aed9-9dd05f317336/dqc_reference
[2024-01-24 13:35:28,766] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:35:28,767] [INFO] Task started: Prodigal
[2024-01-24 13:35:28,767] [INFO] Running command: gunzip -c /var/lib/cwl/stg7cbcd4fb-7099-44e3-97fa-9f27ed09d330/GCF_007996985.1_ASM799698v1_genomic.fna.gz | prodigal -d GCF_007996985.1_ASM799698v1_genomic.fna/cds.fna -a GCF_007996985.1_ASM799698v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:35:39,334] [INFO] Task succeeded: Prodigal
[2024-01-24 13:35:39,334] [INFO] Task started: HMMsearch
[2024-01-24 13:35:39,335] [INFO] Running command: hmmsearch --tblout GCF_007996985.1_ASM799698v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg24590a6f-b3e0-4218-aed9-9dd05f317336/dqc_reference/reference_markers.hmm GCF_007996985.1_ASM799698v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:35:39,637] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:35:39,639] [INFO] Found 6/6 markers.
[2024-01-24 13:35:39,671] [INFO] Query marker FASTA was written to GCF_007996985.1_ASM799698v1_genomic.fna/markers.fasta
[2024-01-24 13:35:39,671] [INFO] Task started: Blastn
[2024-01-24 13:35:39,672] [INFO] Running command: blastn -query GCF_007996985.1_ASM799698v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg24590a6f-b3e0-4218-aed9-9dd05f317336/dqc_reference/reference_markers.fasta -out GCF_007996985.1_ASM799698v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:35:40,656] [INFO] Task succeeded: Blastn
[2024-01-24 13:35:40,660] [INFO] Selected 14 target genomes.
[2024-01-24 13:35:40,660] [INFO] Target genome list was writen to GCF_007996985.1_ASM799698v1_genomic.fna/target_genomes.txt
[2024-01-24 13:35:40,684] [INFO] Task started: fastANI
[2024-01-24 13:35:40,685] [INFO] Running command: fastANI --query /var/lib/cwl/stg7cbcd4fb-7099-44e3-97fa-9f27ed09d330/GCF_007996985.1_ASM799698v1_genomic.fna.gz --refList GCF_007996985.1_ASM799698v1_genomic.fna/target_genomes.txt --output GCF_007996985.1_ASM799698v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:35:55,767] [INFO] Task succeeded: fastANI
[2024-01-24 13:35:55,768] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg24590a6f-b3e0-4218-aed9-9dd05f317336/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:35:55,768] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg24590a6f-b3e0-4218-aed9-9dd05f317336/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:35:55,785] [INFO] Found 14 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 13:35:55,785] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:35:55,785] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Extensimonas vulgaris	strain=S4	GCA_007996985.1	1031594	1031594	type	True	100.0	1009	1012	95	conclusive
Extensimonas vulgaris	strain=DSM 100911	GCA_003337425.1	1031594	1031594	type	True	99.9967	1008	1012	95	conclusive
Extensimonas vulgaris	strain=CGMCC 1.10977	GCA_007830535.1	1031594	1031594	type	True	99.9813	1008	1012	95	conclusive
Extensimonas perlucida	strain=HX2-24	GCA_007655255.1	2590786	2590786	type	True	91.2744	810	1012	95	below_threshold
Acidovorax wautersii	strain=DSM 27981	GCA_900113035.1	1177982	1177982	type	True	80.6928	574	1012	95	below_threshold
Hydrogenophaga crocea	strain=BA0156	GCA_011388215.1	2716225	2716225	type	True	80.2664	499	1012	95	below_threshold
Acidovorax kalamii	strain=KNDSW-TSA6	GCA_002245625.1	2004485	2004485	type	True	79.9598	539	1012	95	below_threshold
Verminephrobacter eiseniae	strain=EF01-2	GCA_000015565.1	364317	364317	type	True	79.599	459	1012	95	below_threshold
Simplicispira hankyongi	strain=NY-02	GCA_003570885.1	2315688	2315688	type	True	79.5125	475	1012	95	below_threshold
Variovorax beijingensis	strain=502	GCA_003951285.1	2496117	2496117	type	True	79.4923	500	1012	95	below_threshold
Acidovorax facilis	strain=DSM 649	GCA_023913775.1	12917	12917	type	True	79.4785	514	1012	95	below_threshold
Ottowia testudinis	strain=27C	GCA_017498525.1	2816950	2816950	type	True	79.3758	472	1012	95	below_threshold
Ramlibacter pinisoli	strain=MAH-25	GCA_009758015.1	2682844	2682844	type	True	78.9592	455	1012	95	below_threshold
Rhodoferax lacus	strain=IMCC26218	GCA_003415675.1	2184758	2184758	type	True	78.3157	346	1012	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:35:55,787] [INFO] DFAST Taxonomy check result was written to GCF_007996985.1_ASM799698v1_genomic.fna/tc_result.tsv
[2024-01-24 13:35:55,787] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:35:55,787] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:35:55,788] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg24590a6f-b3e0-4218-aed9-9dd05f317336/dqc_reference/checkm_data
[2024-01-24 13:35:55,789] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:35:55,819] [INFO] Task started: CheckM
[2024-01-24 13:35:55,820] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_007996985.1_ASM799698v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_007996985.1_ASM799698v1_genomic.fna/checkm_input GCF_007996985.1_ASM799698v1_genomic.fna/checkm_result
[2024-01-24 13:36:30,637] [INFO] Task succeeded: CheckM
[2024-01-24 13:36:30,638] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 97.92%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:36:30,662] [INFO] ===== Completeness check finished =====
[2024-01-24 13:36:30,663] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:36:30,663] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_007996985.1_ASM799698v1_genomic.fna/markers.fasta)
[2024-01-24 13:36:30,664] [INFO] Task started: Blastn
[2024-01-24 13:36:30,664] [INFO] Running command: blastn -query GCF_007996985.1_ASM799698v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg24590a6f-b3e0-4218-aed9-9dd05f317336/dqc_reference/reference_markers_gtdb.fasta -out GCF_007996985.1_ASM799698v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:36:32,692] [INFO] Task succeeded: Blastn
[2024-01-24 13:36:32,697] [INFO] Selected 19 target genomes.
[2024-01-24 13:36:32,697] [INFO] Target genome list was writen to GCF_007996985.1_ASM799698v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:36:32,736] [INFO] Task started: fastANI
[2024-01-24 13:36:32,737] [INFO] Running command: fastANI --query /var/lib/cwl/stg7cbcd4fb-7099-44e3-97fa-9f27ed09d330/GCF_007996985.1_ASM799698v1_genomic.fna.gz --refList GCF_007996985.1_ASM799698v1_genomic.fna/target_genomes_gtdb.txt --output GCF_007996985.1_ASM799698v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:36:49,854] [INFO] Task succeeded: fastANI
[2024-01-24 13:36:49,871] [INFO] Found 19 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:36:49,872] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003337425.1	s__Extensimonas vulgaris	99.9967	1008	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Extensimonas	95.0	99.99	99.99	0.99	0.99	3	conclusive
GCF_007655255.1	s__Extensimonas perlucida	91.2807	809	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Extensimonas	95.0	97.84	97.67	0.89	0.88	3	-
GCF_000204645.1	s__Alicycliphilus denitrificans	81.3087	604	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Alicycliphilus	95.0	97.26	95.80	0.83	0.76	8	-
GCF_000739995.1	s__Giesbergeria granuli	81.2708	545	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Giesbergeria	95.0	95.66	95.66	0.85	0.85	2	-
GCF_003604195.1	s__Giesbergeria lacusdiani	80.9665	562	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Giesbergeria	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003053545.1	s__Acidovorax sp003053545	80.5981	577	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Acidovorax	95.0	98.66	97.32	0.95	0.90	3	-
GCF_002176815.1	s__Acidovorax sp002176815	80.4159	525	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Acidovorax	95.0	98.23	97.20	0.91	0.82	4	-
GCA_001770955.1	s__Giesbergeria sp001770955	80.1996	486	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Giesbergeria	95.0	97.11	95.02	0.83	0.80	5	-
GCA_001724785.1	s__Giesbergeria sp001724785	80.089	402	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Giesbergeria	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013693755.1	s__Tibeticola sp013693755	80.059	488	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Tibeticola	95.0	96.98	96.98	0.89	0.89	2	-
GCF_001633105.1	s__Acidovorax sp001633105	80.0227	557	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Acidovorax	95.0	N/A	N/A	N/A	N/A	1	-
GCA_011620245.1	s__Acidovorax sp011620245	79.938	334	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Acidovorax	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002245625.1	s__Acidovorax kalamii	79.9317	543	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Acidovorax	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001714725.1	s__Acidovorax sp001714725	79.6482	507	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Acidovorax	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003951285.1	s__Variovorax beijingensis	79.5037	498	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Variovorax	95.0	99.27	99.27	0.92	0.92	2	-
GCF_000463015.1	s__Variovorax paradoxus_E	79.3348	496	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Variovorax	95.0	98.39	98.39	0.87	0.87	2	-
GCF_004363315.1	s__Tepidicella xavieri	79.1137	343	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Tepidicella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013432195.1	s__Tepidicella baoligensis	79.113	338	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Tepidicella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007556755.1	s__Tepidimonas fonticaldi	79.0597	399	1012	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Tepidimonas	95.0	97.94	97.94	0.88	0.88	2	-
--------------------------------------------------------------------------------
[2024-01-24 13:36:49,874] [INFO] GTDB search result was written to GCF_007996985.1_ASM799698v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:36:49,875] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:36:49,880] [INFO] DFAST_QC result json was written to GCF_007996985.1_ASM799698v1_genomic.fna/dqc_result.json
[2024-01-24 13:36:49,880] [INFO] DFAST_QC completed!
[2024-01-24 13:36:49,881] [INFO] Total running time: 0h1m22s