[2024-01-24 12:30:05,384] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:30:05,386] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:30:05,386] [INFO] DQC Reference Directory: /var/lib/cwl/stga474bd9f-61e8-4574-a385-e346b0a98ed8/dqc_reference
[2024-01-24 12:30:07,246] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:30:07,247] [INFO] Task started: Prodigal
[2024-01-24 12:30:07,247] [INFO] Running command: gunzip -c /var/lib/cwl/stg9452e830-6ead-4c73-b647-55f1bc157539/GCF_008086615.1_ASM808661v1_genomic.fna.gz | prodigal -d GCF_008086615.1_ASM808661v1_genomic.fna/cds.fna -a GCF_008086615.1_ASM808661v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:30:14,255] [INFO] Task succeeded: Prodigal
[2024-01-24 12:30:14,255] [INFO] Task started: HMMsearch
[2024-01-24 12:30:14,255] [INFO] Running command: hmmsearch --tblout GCF_008086615.1_ASM808661v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga474bd9f-61e8-4574-a385-e346b0a98ed8/dqc_reference/reference_markers.hmm GCF_008086615.1_ASM808661v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:30:14,540] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:30:14,541] [INFO] Found 6/6 markers.
[2024-01-24 12:30:14,580] [INFO] Query marker FASTA was written to GCF_008086615.1_ASM808661v1_genomic.fna/markers.fasta
[2024-01-24 12:30:14,580] [INFO] Task started: Blastn
[2024-01-24 12:30:14,580] [INFO] Running command: blastn -query GCF_008086615.1_ASM808661v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga474bd9f-61e8-4574-a385-e346b0a98ed8/dqc_reference/reference_markers.fasta -out GCF_008086615.1_ASM808661v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:30:15,111] [INFO] Task succeeded: Blastn
[2024-01-24 12:30:15,114] [INFO] Selected 16 target genomes.
[2024-01-24 12:30:15,114] [INFO] Target genome list was writen to GCF_008086615.1_ASM808661v1_genomic.fna/target_genomes.txt
[2024-01-24 12:30:15,152] [INFO] Task started: fastANI
[2024-01-24 12:30:15,152] [INFO] Running command: fastANI --query /var/lib/cwl/stg9452e830-6ead-4c73-b647-55f1bc157539/GCF_008086615.1_ASM808661v1_genomic.fna.gz --refList GCF_008086615.1_ASM808661v1_genomic.fna/target_genomes.txt --output GCF_008086615.1_ASM808661v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:30:23,651] [INFO] Task succeeded: fastANI
[2024-01-24 12:30:23,651] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga474bd9f-61e8-4574-a385-e346b0a98ed8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:30:23,652] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga474bd9f-61e8-4574-a385-e346b0a98ed8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:30:23,659] [INFO] Found 6 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 12:30:23,659] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-24 12:30:23,659] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Acetobacterium paludosum	strain=DSM 8237	GCA_008086595.1	52693	52693	type	True	94.8169	926	1155	95	below_threshold
Acetobacterium woodii	strain=DSM 1030	GCA_000247605.1	33952	33952	type	True	79.0539	355	1155	95	below_threshold
Acetobacterium bakii	strain=DSM 8239	GCA_001263355.1	52689	52689	type	True	78.8252	320	1155	95	below_threshold
Acetobacterium bakii	strain=DSM 8239	GCA_008086625.1	52689	52689	type	True	78.821	330	1155	95	below_threshold
Acetobacterium dehalogenans	strain=DSM 11527	GCA_000472665.1	82116	82116	type	True	78.7471	327	1155	95	below_threshold
Acetobacterium wieringae	strain=DSM 1911	GCA_001766835.1	52694	52694	type	True	78.2186	298	1155	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:30:23,660] [INFO] DFAST Taxonomy check result was written to GCF_008086615.1_ASM808661v1_genomic.fna/tc_result.tsv
[2024-01-24 12:30:23,660] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:30:23,661] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:30:23,661] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga474bd9f-61e8-4574-a385-e346b0a98ed8/dqc_reference/checkm_data
[2024-01-24 12:30:23,662] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:30:23,698] [INFO] Task started: CheckM
[2024-01-24 12:30:23,699] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_008086615.1_ASM808661v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_008086615.1_ASM808661v1_genomic.fna/checkm_input GCF_008086615.1_ASM808661v1_genomic.fna/checkm_result
[2024-01-24 12:30:49,749] [INFO] Task succeeded: CheckM
[2024-01-24 12:30:49,750] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:30:49,814] [INFO] ===== Completeness check finished =====
[2024-01-24 12:30:49,814] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:30:49,814] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_008086615.1_ASM808661v1_genomic.fna/markers.fasta)
[2024-01-24 12:30:49,814] [INFO] Task started: Blastn
[2024-01-24 12:30:49,814] [INFO] Running command: blastn -query GCF_008086615.1_ASM808661v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga474bd9f-61e8-4574-a385-e346b0a98ed8/dqc_reference/reference_markers_gtdb.fasta -out GCF_008086615.1_ASM808661v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:30:50,564] [INFO] Task succeeded: Blastn
[2024-01-24 12:30:50,568] [INFO] Selected 17 target genomes.
[2024-01-24 12:30:50,568] [INFO] Target genome list was writen to GCF_008086615.1_ASM808661v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:30:50,598] [INFO] Task started: fastANI
[2024-01-24 12:30:50,599] [INFO] Running command: fastANI --query /var/lib/cwl/stg9452e830-6ead-4c73-b647-55f1bc157539/GCF_008086615.1_ASM808661v1_genomic.fna.gz --refList GCF_008086615.1_ASM808661v1_genomic.fna/target_genomes_gtdb.txt --output GCF_008086615.1_ASM808661v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:31:00,391] [INFO] Task succeeded: fastANI
[2024-01-24 12:31:00,398] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:31:00,398] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_008086615.1	s__Acetobacterium tundrae	100.0	1153	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	99.99	99.99	0.99	0.99	2	conclusive
GCF_008086595.1	s__Acetobacterium paludosum	94.802	927	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	100.00	100.00	1.00	1.00	2	-
GCF_014284475.1	s__Acetobacterium fimetarium	80.775	530	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016213825.1	s__Acetobacterium woodii_A	79.1686	387	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000247605.1	s__Acetobacterium woodii	79.0732	355	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001263355.1	s__Acetobacterium bakii	78.8176	319	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	99.99	99.99	1.00	1.00	2	-
GCF_000472665.1	s__Acetobacterium dehalogenans	78.7297	328	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	96.75	96.75	0.83	0.83	2	-
GCF_003260995.1	s__Acetobacterium sp003260995	78.4048	306	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	98.25	98.25	0.82	0.82	2	-
GCA_002841405.1	s__Acetobacterium sp002841405	77.7784	259	1155	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:31:00,399] [INFO] GTDB search result was written to GCF_008086615.1_ASM808661v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:31:00,400] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:31:00,402] [INFO] DFAST_QC result json was written to GCF_008086615.1_ASM808661v1_genomic.fna/dqc_result.json
[2024-01-24 12:31:00,402] [INFO] DFAST_QC completed!
[2024-01-24 12:31:00,402] [INFO] Total running time: 0h0m55s