[2024-01-24 13:19:01,336] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:19:01,337] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:19:01,337] [INFO] DQC Reference Directory: /var/lib/cwl/stgb5930ce1-3573-4a18-aa4a-31c201842d0a/dqc_reference
[2024-01-24 13:19:02,658] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:19:02,659] [INFO] Task started: Prodigal
[2024-01-24 13:19:02,659] [INFO] Running command: gunzip -c /var/lib/cwl/stg30417e0c-c7cc-46ab-b294-1d8a21de6aca/GCF_008086625.1_ASM808662v1_genomic.fna.gz | prodigal -d GCF_008086625.1_ASM808662v1_genomic.fna/cds.fna -a GCF_008086625.1_ASM808662v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:19:13,177] [INFO] Task succeeded: Prodigal
[2024-01-24 13:19:13,177] [INFO] Task started: HMMsearch
[2024-01-24 13:19:13,177] [INFO] Running command: hmmsearch --tblout GCF_008086625.1_ASM808662v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb5930ce1-3573-4a18-aa4a-31c201842d0a/dqc_reference/reference_markers.hmm GCF_008086625.1_ASM808662v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:19:13,492] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:19:13,493] [INFO] Found 6/6 markers.
[2024-01-24 13:19:13,542] [INFO] Query marker FASTA was written to GCF_008086625.1_ASM808662v1_genomic.fna/markers.fasta
[2024-01-24 13:19:13,543] [INFO] Task started: Blastn
[2024-01-24 13:19:13,543] [INFO] Running command: blastn -query GCF_008086625.1_ASM808662v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb5930ce1-3573-4a18-aa4a-31c201842d0a/dqc_reference/reference_markers.fasta -out GCF_008086625.1_ASM808662v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:19:14,151] [INFO] Task succeeded: Blastn
[2024-01-24 13:19:14,155] [INFO] Selected 14 target genomes.
[2024-01-24 13:19:14,155] [INFO] Target genome list was writen to GCF_008086625.1_ASM808662v1_genomic.fna/target_genomes.txt
[2024-01-24 13:19:14,168] [INFO] Task started: fastANI
[2024-01-24 13:19:14,169] [INFO] Running command: fastANI --query /var/lib/cwl/stg30417e0c-c7cc-46ab-b294-1d8a21de6aca/GCF_008086625.1_ASM808662v1_genomic.fna.gz --refList GCF_008086625.1_ASM808662v1_genomic.fna/target_genomes.txt --output GCF_008086625.1_ASM808662v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:19:24,733] [INFO] Task succeeded: fastANI
[2024-01-24 13:19:24,733] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb5930ce1-3573-4a18-aa4a-31c201842d0a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:19:24,733] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb5930ce1-3573-4a18-aa4a-31c201842d0a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:19:24,741] [INFO] Found 8 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 13:19:24,741] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:19:24,741] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Acetobacterium bakii	strain=DSM 8239	GCA_008086625.1	52689	52689	type	True	100.0	1367	1369	95	conclusive
Acetobacterium bakii	strain=DSM 8239	GCA_001263355.1	52689	52689	type	True	99.9659	1320	1369	95	conclusive
Acetobacterium paludosum	strain=DSM 8237	GCA_008086595.1	52693	52693	type	True	78.7704	372	1369	95	below_threshold
Acetobacterium dehalogenans	strain=DSM 11527	GCA_000472665.1	82116	82116	type	True	78.6868	354	1369	95	below_threshold
Acetobacterium wieringae	strain=DSM 1911	GCA_001766835.1	52694	52694	type	True	78.1631	328	1369	95	below_threshold
Acetobacterium woodii	strain=DSM 1030	GCA_000247605.1	33952	33952	type	True	77.9711	338	1369	95	below_threshold
Eubacterium limosum	strain=ATCC 8486	GCA_000807675.2	1736	1736	type	True	76.4246	50	1369	95	below_threshold
Eubacterium callanderi	strain=FD	GCA_900142645.1	53442	53442	type	True	76.0337	54	1369	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:19:24,745] [INFO] DFAST Taxonomy check result was written to GCF_008086625.1_ASM808662v1_genomic.fna/tc_result.tsv
[2024-01-24 13:19:24,747] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:19:24,747] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:19:24,747] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb5930ce1-3573-4a18-aa4a-31c201842d0a/dqc_reference/checkm_data
[2024-01-24 13:19:24,748] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:19:24,791] [INFO] Task started: CheckM
[2024-01-24 13:19:24,791] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_008086625.1_ASM808662v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_008086625.1_ASM808662v1_genomic.fna/checkm_input GCF_008086625.1_ASM808662v1_genomic.fna/checkm_result
[2024-01-24 13:20:00,792] [INFO] Task succeeded: CheckM
[2024-01-24 13:20:00,793] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:20:00,810] [INFO] ===== Completeness check finished =====
[2024-01-24 13:20:00,810] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:20:00,811] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_008086625.1_ASM808662v1_genomic.fna/markers.fasta)
[2024-01-24 13:20:00,812] [INFO] Task started: Blastn
[2024-01-24 13:20:00,812] [INFO] Running command: blastn -query GCF_008086625.1_ASM808662v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb5930ce1-3573-4a18-aa4a-31c201842d0a/dqc_reference/reference_markers_gtdb.fasta -out GCF_008086625.1_ASM808662v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:20:01,670] [INFO] Task succeeded: Blastn
[2024-01-24 13:20:01,673] [INFO] Selected 16 target genomes.
[2024-01-24 13:20:01,674] [INFO] Target genome list was writen to GCF_008086625.1_ASM808662v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:20:01,713] [INFO] Task started: fastANI
[2024-01-24 13:20:01,714] [INFO] Running command: fastANI --query /var/lib/cwl/stg30417e0c-c7cc-46ab-b294-1d8a21de6aca/GCF_008086625.1_ASM808662v1_genomic.fna.gz --refList GCF_008086625.1_ASM808662v1_genomic.fna/target_genomes_gtdb.txt --output GCF_008086625.1_ASM808662v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:20:12,209] [INFO] Task succeeded: fastANI
[2024-01-24 13:20:12,223] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:20:12,223] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001263355.1	s__Acetobacterium bakii	99.9659	1320	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	99.99	99.99	1.00	1.00	2	conclusive
GCA_016650045.1	s__Acetobacterium sp016650045	80.6628	371	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003260995.1	s__Acetobacterium sp003260995	79.0385	363	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	98.25	98.25	0.82	0.82	2	-
GCF_008086615.1	s__Acetobacterium tundrae	78.9351	318	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	99.99	99.99	0.99	0.99	2	-
GCF_008086595.1	s__Acetobacterium paludosum	78.7707	372	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	100.00	100.00	1.00	1.00	2	-
GCF_000472665.1	s__Acetobacterium dehalogenans	78.6639	356	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	96.75	96.75	0.83	0.83	2	-
GCF_014284475.1	s__Acetobacterium fimetarium	78.6318	307	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002841405.1	s__Acetobacterium sp002841405	78.5566	304	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016213825.1	s__Acetobacterium woodii_A	78.5014	353	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001766835.1	s__Acetobacterium wieringae	78.1577	328	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	97.41	97.27	0.88	0.86	5	-
GCA_018903255.1	s__Acetobacterium sp018903255	77.8536	242	1369	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Eubacteriales;f__Eubacteriaceae;g__Acetobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:20:12,225] [INFO] GTDB search result was written to GCF_008086625.1_ASM808662v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:20:12,225] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:20:12,228] [INFO] DFAST_QC result json was written to GCF_008086625.1_ASM808662v1_genomic.fna/dqc_result.json
[2024-01-24 13:20:12,228] [INFO] DFAST_QC completed!
[2024-01-24 13:20:12,228] [INFO] Total running time: 0h1m11s