[2024-01-24 14:12:35,072] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:12:35,074] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:12:35,074] [INFO] DQC Reference Directory: /var/lib/cwl/stg99a2d9d2-8f9f-4c9c-9d17-b225a554ae80/dqc_reference
[2024-01-24 14:12:36,556] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:12:36,557] [INFO] Task started: Prodigal
[2024-01-24 14:12:36,557] [INFO] Running command: gunzip -c /var/lib/cwl/stg219d9b7c-46ef-42aa-ba2e-6b3c0adc2f27/GCF_008801525.1_ASM880152v1_genomic.fna.gz | prodigal -d GCF_008801525.1_ASM880152v1_genomic.fna/cds.fna -a GCF_008801525.1_ASM880152v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:12:59,373] [INFO] Task succeeded: Prodigal
[2024-01-24 14:12:59,373] [INFO] Task started: HMMsearch
[2024-01-24 14:12:59,373] [INFO] Running command: hmmsearch --tblout GCF_008801525.1_ASM880152v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg99a2d9d2-8f9f-4c9c-9d17-b225a554ae80/dqc_reference/reference_markers.hmm GCF_008801525.1_ASM880152v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:12:59,743] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:12:59,744] [INFO] Found 6/6 markers.
[2024-01-24 14:12:59,798] [INFO] Query marker FASTA was written to GCF_008801525.1_ASM880152v1_genomic.fna/markers.fasta
[2024-01-24 14:12:59,798] [INFO] Task started: Blastn
[2024-01-24 14:12:59,799] [INFO] Running command: blastn -query GCF_008801525.1_ASM880152v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg99a2d9d2-8f9f-4c9c-9d17-b225a554ae80/dqc_reference/reference_markers.fasta -out GCF_008801525.1_ASM880152v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:13:00,846] [INFO] Task succeeded: Blastn
[2024-01-24 14:13:00,850] [INFO] Selected 12 target genomes.
[2024-01-24 14:13:00,850] [INFO] Target genome list was writen to GCF_008801525.1_ASM880152v1_genomic.fna/target_genomes.txt
[2024-01-24 14:13:00,857] [INFO] Task started: fastANI
[2024-01-24 14:13:00,858] [INFO] Running command: fastANI --query /var/lib/cwl/stg219d9b7c-46ef-42aa-ba2e-6b3c0adc2f27/GCF_008801525.1_ASM880152v1_genomic.fna.gz --refList GCF_008801525.1_ASM880152v1_genomic.fna/target_genomes.txt --output GCF_008801525.1_ASM880152v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:13:22,417] [INFO] Task succeeded: fastANI
[2024-01-24 14:13:22,418] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg99a2d9d2-8f9f-4c9c-9d17-b225a554ae80/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:13:22,419] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg99a2d9d2-8f9f-4c9c-9d17-b225a554ae80/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:13:22,436] [INFO] Found 12 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 14:13:22,436] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:13:22,437] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Pseudomonas lini	strain=CCUG 51522	GCA_008801525.1	163011	163011	type	True	100.0	2149	2150	95	conclusive
Pseudomonas lini	strain=DSM 16768	GCA_001042905.1	163011	163011	type	True	99.9991	2143	2150	95	conclusive
Pseudomonas farris	strain=SWRI79	GCA_019145235.1	2841207	2841207	type	True	94.9213	1809	2150	95	below_threshold
Pseudomonas mandelii	strain=NBRC 103147	GCA_002091695.1	75612	75612	suspected-type	True	88.2872	1581	2150	95	below_threshold
Pseudomonas mandelii	strain=DSM 17967	GCA_007858265.1	75612	75612	suspected-type	True	88.2852	1614	2150	95	below_threshold
Pseudomonas laurylsulfatiphila	strain=AP3_16	GCA_002934665.1	2011015	2011015	type	True	86.7751	1447	2150	95	below_threshold
Pseudomonas laurylsulfativorans	strain=AP3_22	GCA_002906155.1	1943631	1943631	type	True	86.6336	1414	2150	95	below_threshold
Pseudomonas izuensis	strain=IzPS43_3003	GCA_009861505.1	2684212	2684212	type	True	86.6115	1426	2150	95	below_threshold
Pseudomonas pharyngis	strain=BML-PP036	GCA_021602345.1	2892333	2892333	type	True	86.1414	1442	2150	95	below_threshold
Pseudomonas sputi	strain=BML-PP014	GCA_021603585.1	2892325	2892325	type	True	86.0081	1432	2150	95	below_threshold
Pseudomonas germanica	strain=FIT28	GCA_019614655.1	2815720	2815720	type	True	85.3696	1506	2150	95	below_threshold
Pseudomonas rhizophila	strain=S211	GCA_003033885.1	2045200	2045200	type	True	84.1218	1381	2150	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:13:22,438] [INFO] DFAST Taxonomy check result was written to GCF_008801525.1_ASM880152v1_genomic.fna/tc_result.tsv
[2024-01-24 14:13:22,439] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:13:22,439] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:13:22,440] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg99a2d9d2-8f9f-4c9c-9d17-b225a554ae80/dqc_reference/checkm_data
[2024-01-24 14:13:22,441] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:13:22,499] [INFO] Task started: CheckM
[2024-01-24 14:13:22,499] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_008801525.1_ASM880152v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_008801525.1_ASM880152v1_genomic.fna/checkm_input GCF_008801525.1_ASM880152v1_genomic.fna/checkm_result
[2024-01-24 14:14:24,556] [INFO] Task succeeded: CheckM
[2024-01-24 14:14:24,561] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:14:24,585] [INFO] ===== Completeness check finished =====
[2024-01-24 14:14:24,586] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:14:24,586] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_008801525.1_ASM880152v1_genomic.fna/markers.fasta)
[2024-01-24 14:14:24,586] [INFO] Task started: Blastn
[2024-01-24 14:14:24,587] [INFO] Running command: blastn -query GCF_008801525.1_ASM880152v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg99a2d9d2-8f9f-4c9c-9d17-b225a554ae80/dqc_reference/reference_markers_gtdb.fasta -out GCF_008801525.1_ASM880152v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:14:26,253] [INFO] Task succeeded: Blastn
[2024-01-24 14:14:26,258] [INFO] Selected 7 target genomes.
[2024-01-24 14:14:26,258] [INFO] Target genome list was writen to GCF_008801525.1_ASM880152v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:14:26,282] [INFO] Task started: fastANI
[2024-01-24 14:14:26,282] [INFO] Running command: fastANI --query /var/lib/cwl/stg219d9b7c-46ef-42aa-ba2e-6b3c0adc2f27/GCF_008801525.1_ASM880152v1_genomic.fna.gz --refList GCF_008801525.1_ASM880152v1_genomic.fna/target_genomes_gtdb.txt --output GCF_008801525.1_ASM880152v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:14:41,990] [INFO] Task succeeded: fastANI
[2024-01-24 14:14:41,998] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:14:41,998] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001042905.1	s__Pseudomonas_E lini	99.9991	2143	2150	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	99.33	98.85	0.97	0.92	6	conclusive
GCF_001238485.1	s__Pseudomonas_E syringae_E	94.6231	1766	2150	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	95.97	95.90	0.85	0.84	15	-
GCF_003732275.1	s__Pseudomonas_E frederiksbergensis_H	94.447	1788	2150	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000282375.1	s__Pseudomonas_E sp000282375	94.0407	1734	2150	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	96.36	96.36	0.86	0.86	2	-
GCF_000282495.1	s__Pseudomonas_E sp000282495	93.8495	1756	2150	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	96.86	95.96	0.87	0.86	6	-
GCF_000512695.2	s__Pseudomonas_E sp000512695	93.7508	1600	2150	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900187445.1	s__Pseudomonas_E sp900187445	93.5229	1666	2150	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 14:14:42,000] [INFO] GTDB search result was written to GCF_008801525.1_ASM880152v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:14:42,000] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:14:42,003] [INFO] DFAST_QC result json was written to GCF_008801525.1_ASM880152v1_genomic.fna/dqc_result.json
[2024-01-24 14:14:42,003] [INFO] DFAST_QC completed!
[2024-01-24 14:14:42,003] [INFO] Total running time: 0h2m7s