[2024-01-24 13:01:52,503] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:01:52,505] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:01:52,505] [INFO] DQC Reference Directory: /var/lib/cwl/stgf0ea15c9-095d-4568-88a9-f0c8ad797782/dqc_reference
[2024-01-24 13:01:53,838] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:01:53,839] [INFO] Task started: Prodigal
[2024-01-24 13:01:53,839] [INFO] Running command: gunzip -c /var/lib/cwl/stg41040610-078e-4818-91a1-ef826cd2a40b/GCF_009806955.1_ASM980695v1_genomic.fna.gz | prodigal -d GCF_009806955.1_ASM980695v1_genomic.fna/cds.fna -a GCF_009806955.1_ASM980695v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:02:03,105] [INFO] Task succeeded: Prodigal
[2024-01-24 13:02:03,105] [INFO] Task started: HMMsearch
[2024-01-24 13:02:03,105] [INFO] Running command: hmmsearch --tblout GCF_009806955.1_ASM980695v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf0ea15c9-095d-4568-88a9-f0c8ad797782/dqc_reference/reference_markers.hmm GCF_009806955.1_ASM980695v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:02:03,418] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:02:03,420] [INFO] Found 6/6 markers.
[2024-01-24 13:02:03,464] [INFO] Query marker FASTA was written to GCF_009806955.1_ASM980695v1_genomic.fna/markers.fasta
[2024-01-24 13:02:03,464] [INFO] Task started: Blastn
[2024-01-24 13:02:03,464] [INFO] Running command: blastn -query GCF_009806955.1_ASM980695v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf0ea15c9-095d-4568-88a9-f0c8ad797782/dqc_reference/reference_markers.fasta -out GCF_009806955.1_ASM980695v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:02:04,522] [INFO] Task succeeded: Blastn
[2024-01-24 13:02:04,526] [INFO] Selected 13 target genomes.
[2024-01-24 13:02:04,526] [INFO] Target genome list was writen to GCF_009806955.1_ASM980695v1_genomic.fna/target_genomes.txt
[2024-01-24 13:02:04,533] [INFO] Task started: fastANI
[2024-01-24 13:02:04,533] [INFO] Running command: fastANI --query /var/lib/cwl/stg41040610-078e-4818-91a1-ef826cd2a40b/GCF_009806955.1_ASM980695v1_genomic.fna.gz --refList GCF_009806955.1_ASM980695v1_genomic.fna/target_genomes.txt --output GCF_009806955.1_ASM980695v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:02:14,128] [INFO] Task succeeded: fastANI
[2024-01-24 13:02:14,129] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf0ea15c9-095d-4568-88a9-f0c8ad797782/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:02:14,129] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf0ea15c9-095d-4568-88a9-f0c8ad797782/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:02:14,146] [INFO] Found 13 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 13:02:14,146] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:02:14,146] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Rathayibacter iranicus	strain=VKM Ac-1602	GCA_009806955.1	59737	59737	type	True	100.0	1099	1102	95	conclusive
Rathayibacter iranicus	strain=VKM Ac-1602	GCA_003149025.1	59737	59737	type	True	99.9924	1092	1102	95	conclusive
Rathayibacter iranicus	strain=DSM 7484	GCA_002933035.1	59737	59737	type	True	99.9794	1082	1102	95	conclusive
Rathayibacter agropyri	strain=VKM Ac-2828	GCA_013249015.1	1634927	1634927	type	True	90.061	883	1102	95	below_threshold
Rathayibacter tritici	strain=DSM 7486	GCA_002932875.1	33888	33888	type	True	84.6488	756	1102	95	below_threshold
Rathayibacter tanaceti	strain=VKM Ac-2596	GCA_001634385.1	1671680	1671680	type	True	84.3499	652	1102	95	below_threshold
Rathayibacter tanaceti	strain=VKM Ac-2596	GCA_004340625.1	1671680	1671680	type	True	84.2797	751	1102	95	below_threshold
Rathayibacter rathayi	strain=VKM Ac-1601	GCA_900215645.1	33887	33887	type	True	84.2599	780	1102	95	below_threshold
Rathayibacter oskolensis	strain=VKM Ac-2121	GCA_900177245.1	1891671	1891671	type	True	82.9278	698	1102	95	below_threshold
Agromyces archimandritae	strain=G127AT	GCA_018024495.1	2781962	2781962	type	True	78.3235	284	1102	95	below_threshold
Agromyces mariniharenae	strain=NEAU-184	GCA_008122505.1	2604423	2604423	type	True	78.3141	328	1102	95	below_threshold
Agromyces marinus	strain=DSM 26151	GCA_021442325.1	1389020	1389020	type	True	78.1111	288	1102	95	below_threshold
Agromyces humatus	strain=JCM 14319	GCA_021228295.1	279573	279573	type	True	78.0386	291	1102	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:02:14,148] [INFO] DFAST Taxonomy check result was written to GCF_009806955.1_ASM980695v1_genomic.fna/tc_result.tsv
[2024-01-24 13:02:14,148] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:02:14,148] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:02:14,149] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf0ea15c9-095d-4568-88a9-f0c8ad797782/dqc_reference/checkm_data
[2024-01-24 13:02:14,150] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:02:14,185] [INFO] Task started: CheckM
[2024-01-24 13:02:14,186] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_009806955.1_ASM980695v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_009806955.1_ASM980695v1_genomic.fna/checkm_input GCF_009806955.1_ASM980695v1_genomic.fna/checkm_result
[2024-01-24 13:02:45,945] [INFO] Task succeeded: CheckM
[2024-01-24 13:02:45,946] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:02:45,968] [INFO] ===== Completeness check finished =====
[2024-01-24 13:02:45,969] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:02:45,969] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_009806955.1_ASM980695v1_genomic.fna/markers.fasta)
[2024-01-24 13:02:45,970] [INFO] Task started: Blastn
[2024-01-24 13:02:45,970] [INFO] Running command: blastn -query GCF_009806955.1_ASM980695v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf0ea15c9-095d-4568-88a9-f0c8ad797782/dqc_reference/reference_markers_gtdb.fasta -out GCF_009806955.1_ASM980695v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:02:47,498] [INFO] Task succeeded: Blastn
[2024-01-24 13:02:47,505] [INFO] Selected 11 target genomes.
[2024-01-24 13:02:47,506] [INFO] Target genome list was writen to GCF_009806955.1_ASM980695v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:02:47,515] [INFO] Task started: fastANI
[2024-01-24 13:02:47,516] [INFO] Running command: fastANI --query /var/lib/cwl/stg41040610-078e-4818-91a1-ef826cd2a40b/GCF_009806955.1_ASM980695v1_genomic.fna.gz --refList GCF_009806955.1_ASM980695v1_genomic.fna/target_genomes_gtdb.txt --output GCF_009806955.1_ASM980695v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:02:56,475] [INFO] Task succeeded: fastANI
[2024-01-24 13:02:56,485] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:02:56,485] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003149025.1	s__Rathayibacter iranicus	99.9924	1092	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	99.96	99.91	0.99	0.99	6	conclusive
GCF_013249015.1	s__Rathayibacter agropyri	90.016	886	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009866585.1	s__Rathayibacter sp002930885	85.1627	765	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	98.23	95.98	0.89	0.85	55	-
GCF_002932875.1	s__Rathayibacter tritici	84.6861	754	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	99.66	99.47	0.96	0.94	6	-
GCF_001634385.1	s__Rathayibacter tanaceti	84.3455	652	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	99.97	99.97	1.00	1.00	3	-
GCF_004011095.1	s__Rathayibacter rathayi	84.2907	774	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	99.62	99.25	0.95	0.91	27	-
GCF_009866655.1	s__Rathayibacter sp009866655	83.4302	714	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	98.89	98.89	0.92	0.92	2	-
GCF_013205025.1	s__Rathayibacter sp013205025	83.4283	720	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009834225.1	s__Rathayibacter sp009834225	83.3881	716	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009834185.1	s__Rathayibacter sp009834185	83.3058	735	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009807095.1	s__Rathayibacter sp009807095	83.2852	705	1102	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Rathayibacter	95.0	99.31	99.31	0.97	0.97	2	-
--------------------------------------------------------------------------------
[2024-01-24 13:02:56,487] [INFO] GTDB search result was written to GCF_009806955.1_ASM980695v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:02:56,487] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:02:56,491] [INFO] DFAST_QC result json was written to GCF_009806955.1_ASM980695v1_genomic.fna/dqc_result.json
[2024-01-24 13:02:56,491] [INFO] DFAST_QC completed!
[2024-01-24 13:02:56,491] [INFO] Total running time: 0h1m4s