[2024-01-24 13:17:30,991] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:17:30,993] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:17:30,993] [INFO] DQC Reference Directory: /var/lib/cwl/stgb0f08daf-7f0c-468d-ad81-02bb2c3b0683/dqc_reference
[2024-01-24 13:17:32,242] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:17:32,243] [INFO] Task started: Prodigal
[2024-01-24 13:17:32,244] [INFO] Running command: gunzip -c /var/lib/cwl/stg936f2361-0a77-48fa-ab44-933a4e9bcbcf/GCF_010586825.1_ASM1058682v1_genomic.fna.gz | prodigal -d GCF_010586825.1_ASM1058682v1_genomic.fna/cds.fna -a GCF_010586825.1_ASM1058682v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:18:00,132] [INFO] Task succeeded: Prodigal
[2024-01-24 13:18:00,132] [INFO] Task started: HMMsearch
[2024-01-24 13:18:00,132] [INFO] Running command: hmmsearch --tblout GCF_010586825.1_ASM1058682v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb0f08daf-7f0c-468d-ad81-02bb2c3b0683/dqc_reference/reference_markers.hmm GCF_010586825.1_ASM1058682v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:18:00,483] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:18:00,484] [INFO] Found 6/6 markers.
[2024-01-24 13:18:00,535] [INFO] Query marker FASTA was written to GCF_010586825.1_ASM1058682v1_genomic.fna/markers.fasta
[2024-01-24 13:18:00,535] [INFO] Task started: Blastn
[2024-01-24 13:18:00,536] [INFO] Running command: blastn -query GCF_010586825.1_ASM1058682v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb0f08daf-7f0c-468d-ad81-02bb2c3b0683/dqc_reference/reference_markers.fasta -out GCF_010586825.1_ASM1058682v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:18:01,191] [INFO] Task succeeded: Blastn
[2024-01-24 13:18:01,196] [INFO] Selected 10 target genomes.
[2024-01-24 13:18:01,197] [INFO] Target genome list was writen to GCF_010586825.1_ASM1058682v1_genomic.fna/target_genomes.txt
[2024-01-24 13:18:01,201] [INFO] Task started: fastANI
[2024-01-24 13:18:01,202] [INFO] Running command: fastANI --query /var/lib/cwl/stg936f2361-0a77-48fa-ab44-933a4e9bcbcf/GCF_010586825.1_ASM1058682v1_genomic.fna.gz --refList GCF_010586825.1_ASM1058682v1_genomic.fna/target_genomes.txt --output GCF_010586825.1_ASM1058682v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:18:12,379] [INFO] Task succeeded: fastANI
[2024-01-24 13:18:12,380] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb0f08daf-7f0c-468d-ad81-02bb2c3b0683/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:18:12,380] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb0f08daf-7f0c-468d-ad81-02bb2c3b0683/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:18:12,388] [INFO] Found 7 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 13:18:12,388] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:18:12,388] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Draconibacterium mangrovi	strain=GM2-18	GCA_010586825.1	2697469	2697469	type	True	100.0	1952	1954	95	conclusive
Draconibacterium sediminis	strain=JN14CK-3	GCA_000949475.1	1544798	1544798	type	True	93.39	1518	1954	95	below_threshold
Draconibacterium orientale	strain=DSM 25947	GCA_900111425.1	1168034	1168034	type	True	87.3657	1288	1954	95	below_threshold
Draconibacterium orientale	strain=FH5	GCA_000626635.1	1168034	1168034	type	True	87.3603	1307	1954	95	below_threshold
Draconibacterium halophilum	strain=M1	GCA_010448835.1	2706887	2706887	type	True	84.1739	1163	1954	95	below_threshold
Maribellus sediminis	strain=Y2-1-60	GCA_011799805.1	2696285	2696285	type	True	79.223	545	1954	95	below_threshold
Mangrovibacterium lignilyticum	strain=BM_7	GCA_010882175.1	2668052	2668052	type	True	76.6704	149	1954	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:18:12,390] [INFO] DFAST Taxonomy check result was written to GCF_010586825.1_ASM1058682v1_genomic.fna/tc_result.tsv
[2024-01-24 13:18:12,390] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:18:12,390] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:18:12,391] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb0f08daf-7f0c-468d-ad81-02bb2c3b0683/dqc_reference/checkm_data
[2024-01-24 13:18:12,392] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:18:12,448] [INFO] Task started: CheckM
[2024-01-24 13:18:12,448] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_010586825.1_ASM1058682v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_010586825.1_ASM1058682v1_genomic.fna/checkm_input GCF_010586825.1_ASM1058682v1_genomic.fna/checkm_result
[2024-01-24 13:19:26,499] [INFO] Task succeeded: CheckM
[2024-01-24 13:19:26,500] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:19:26,520] [INFO] ===== Completeness check finished =====
[2024-01-24 13:19:26,520] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:19:26,520] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_010586825.1_ASM1058682v1_genomic.fna/markers.fasta)
[2024-01-24 13:19:26,521] [INFO] Task started: Blastn
[2024-01-24 13:19:26,521] [INFO] Running command: blastn -query GCF_010586825.1_ASM1058682v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgb0f08daf-7f0c-468d-ad81-02bb2c3b0683/dqc_reference/reference_markers_gtdb.fasta -out GCF_010586825.1_ASM1058682v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:19:27,377] [INFO] Task succeeded: Blastn
[2024-01-24 13:19:27,380] [INFO] Selected 7 target genomes.
[2024-01-24 13:19:27,381] [INFO] Target genome list was writen to GCF_010586825.1_ASM1058682v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:19:27,388] [INFO] Task started: fastANI
[2024-01-24 13:19:27,389] [INFO] Running command: fastANI --query /var/lib/cwl/stg936f2361-0a77-48fa-ab44-933a4e9bcbcf/GCF_010586825.1_ASM1058682v1_genomic.fna.gz --refList GCF_010586825.1_ASM1058682v1_genomic.fna/target_genomes_gtdb.txt --output GCF_010586825.1_ASM1058682v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:19:35,558] [INFO] Task succeeded: fastANI
[2024-01-24 13:19:35,565] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:19:35,565] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_010586825.1	s__Draconibacterium mangrovi	100.0	1952	1954	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Prolixibacteraceae;g__Draconibacterium	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_000949475.1	s__Draconibacterium sediminis	93.4128	1516	1954	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Prolixibacteraceae;g__Draconibacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000626635.1	s__Draconibacterium orientale	87.3502	1308	1954	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Prolixibacteraceae;g__Draconibacterium	95.0	99.98	99.98	1.00	1.00	2	-
GCF_010448835.1	s__Draconibacterium halophilum	84.1656	1164	1954	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Prolixibacteraceae;g__Draconibacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011799805.1	s__Draconibacterium sp011799805	79.2272	544	1954	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Prolixibacteraceae;g__Draconibacterium	95.0	96.71	96.71	0.85	0.85	2	-
GCA_011620585.1	s__Draconibacterium sp011620585	77.3973	299	1954	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Prolixibacteraceae;g__Draconibacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015493625.1	s__Draconibacterium sp015493625	76.3622	152	1954	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Prolixibacteraceae;g__Draconibacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:19:35,567] [INFO] GTDB search result was written to GCF_010586825.1_ASM1058682v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:19:35,568] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:19:35,570] [INFO] DFAST_QC result json was written to GCF_010586825.1_ASM1058682v1_genomic.fna/dqc_result.json
[2024-01-24 13:19:35,571] [INFO] DFAST_QC completed!
[2024-01-24 13:19:35,571] [INFO] Total running time: 0h2m5s