[2024-01-24 13:33:40,904] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:33:40,908] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:33:40,908] [INFO] DQC Reference Directory: /var/lib/cwl/stgfe331fbf-4971-433e-b4d4-1b44af241a77/dqc_reference
[2024-01-24 13:33:42,140] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:33:42,141] [INFO] Task started: Prodigal
[2024-01-24 13:33:42,142] [INFO] Running command: gunzip -c /var/lib/cwl/stgac655f56-a646-435c-962a-257d3ddfac9c/GCF_010667685.1_ASM1066768v1_genomic.fna.gz | prodigal -d GCF_010667685.1_ASM1066768v1_genomic.fna/cds.fna -a GCF_010667685.1_ASM1066768v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:33:51,461] [INFO] Task succeeded: Prodigal
[2024-01-24 13:33:51,462] [INFO] Task started: HMMsearch
[2024-01-24 13:33:51,462] [INFO] Running command: hmmsearch --tblout GCF_010667685.1_ASM1066768v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfe331fbf-4971-433e-b4d4-1b44af241a77/dqc_reference/reference_markers.hmm GCF_010667685.1_ASM1066768v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:33:51,722] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:33:51,724] [INFO] Found 6/6 markers.
[2024-01-24 13:33:51,749] [INFO] Query marker FASTA was written to GCF_010667685.1_ASM1066768v1_genomic.fna/markers.fasta
[2024-01-24 13:33:51,750] [INFO] Task started: Blastn
[2024-01-24 13:33:51,750] [INFO] Running command: blastn -query GCF_010667685.1_ASM1066768v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgfe331fbf-4971-433e-b4d4-1b44af241a77/dqc_reference/reference_markers.fasta -out GCF_010667685.1_ASM1066768v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:33:52,784] [INFO] Task succeeded: Blastn
[2024-01-24 13:33:52,790] [INFO] Selected 14 target genomes.
[2024-01-24 13:33:52,791] [INFO] Target genome list was writen to GCF_010667685.1_ASM1066768v1_genomic.fna/target_genomes.txt
[2024-01-24 13:33:53,036] [INFO] Task started: fastANI
[2024-01-24 13:33:53,036] [INFO] Running command: fastANI --query /var/lib/cwl/stgac655f56-a646-435c-962a-257d3ddfac9c/GCF_010667685.1_ASM1066768v1_genomic.fna.gz --refList GCF_010667685.1_ASM1066768v1_genomic.fna/target_genomes.txt --output GCF_010667685.1_ASM1066768v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:34:02,492] [INFO] Task succeeded: fastANI
[2024-01-24 13:34:02,492] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgfe331fbf-4971-433e-b4d4-1b44af241a77/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:34:02,493] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgfe331fbf-4971-433e-b4d4-1b44af241a77/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:34:02,508] [INFO] Found 14 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 13:34:02,508] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:34:02,509] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Bifidobacterium aerophilum	strain=TRE17	GCA_010667685.1	1798155	1798155	type	True	100.0	969	970	95	conclusive
Bifidobacterium simiiventris	strain=81T8	GCA_019331715.1	2834434	2834434	type	True	89.2181	675	970	95	below_threshold
Bifidobacterium ramosum	strain=DSM 100688	GCA_009299505.1	1798158	1798158	type	True	88.1882	683	970	95	below_threshold
Bifidobacterium ramosum	strain=TREM	GCA_010667665.1	1798158	1798158	type	True	87.9866	700	970	95	below_threshold
Bifidobacterium miconis	strain=82T10	GCA_019331735.1	2834435	2834435	type	True	87.1417	620	970	95	below_threshold
Bifidobacterium biavatii	strain=DSM 23969	GCA_000741165.1	762212	762212	type	True	86.7392	611	970	95	below_threshold
Bifidobacterium amazonense	strain=MA1	GCA_018555435.2	2809027	2809027	type	True	86.6584	613	970	95	below_threshold
Bifidobacterium scardovii	strain=DSM 13734	GCA_000770985.1	158787	158787	type	True	84.3014	542	970	95	below_threshold
Bifidobacterium samirii	strain=2033B	GCA_003952945.1	2306974	2306974	type	True	84.2354	522	970	95	below_threshold
Bifidobacterium aesculapii	strain=DSM 26737	GCA_001417815.1	1329411	1329411	type	True	83.5339	466	970	95	below_threshold
Bifidobacterium miconisargentati	strain=82T25	GCA_019331675.1	2834437	2834437	type	True	82.7524	503	970	95	below_threshold
Bifidobacterium pseudolongum subsp. pseudolongum	strain=ATCC 25526	GCA_022691205.1	31954	1694	type	True	80.2812	298	970	95	below_threshold
Cellulomonas avistercoris	strain=Sa3CUA2	GCA_014836445.1	2762242	2762242	type	True	76.2425	122	970	95	below_threshold
Occultella glacieicola	strain=T3246-1	GCA_004353825.1	2518684	2518684	type	True	75.7625	92	970	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:34:02,510] [INFO] DFAST Taxonomy check result was written to GCF_010667685.1_ASM1066768v1_genomic.fna/tc_result.tsv
[2024-01-24 13:34:02,511] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:34:02,511] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:34:02,511] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfe331fbf-4971-433e-b4d4-1b44af241a77/dqc_reference/checkm_data
[2024-01-24 13:34:02,513] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:34:02,542] [INFO] Task started: CheckM
[2024-01-24 13:34:02,542] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_010667685.1_ASM1066768v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_010667685.1_ASM1066768v1_genomic.fna/checkm_input GCF_010667685.1_ASM1066768v1_genomic.fna/checkm_result
[2024-01-24 13:34:34,112] [INFO] Task succeeded: CheckM
[2024-01-24 13:34:34,113] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:34:34,139] [INFO] ===== Completeness check finished =====
[2024-01-24 13:34:34,140] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:34:34,140] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_010667685.1_ASM1066768v1_genomic.fna/markers.fasta)
[2024-01-24 13:34:34,141] [INFO] Task started: Blastn
[2024-01-24 13:34:34,141] [INFO] Running command: blastn -query GCF_010667685.1_ASM1066768v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgfe331fbf-4971-433e-b4d4-1b44af241a77/dqc_reference/reference_markers_gtdb.fasta -out GCF_010667685.1_ASM1066768v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:34:35,635] [INFO] Task succeeded: Blastn
[2024-01-24 13:34:35,638] [INFO] Selected 8 target genomes.
[2024-01-24 13:34:35,638] [INFO] Target genome list was writen to GCF_010667685.1_ASM1066768v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:34:36,051] [INFO] Task started: fastANI
[2024-01-24 13:34:36,051] [INFO] Running command: fastANI --query /var/lib/cwl/stgac655f56-a646-435c-962a-257d3ddfac9c/GCF_010667685.1_ASM1066768v1_genomic.fna.gz --refList GCF_010667685.1_ASM1066768v1_genomic.fna/target_genomes_gtdb.txt --output GCF_010667685.1_ASM1066768v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:34:41,763] [INFO] Task succeeded: fastANI
[2024-01-24 13:34:41,772] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:34:41,772] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_010667685.1	s__Bifidobacterium aerophilum	100.0	969	970	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_018555335.1	s__Bifidobacterium sp018555335	89.0513	671	970	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009299505.1	s__Bifidobacterium ramosum	88.1665	685	970	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	99.99	99.99	0.99	0.99	2	-
GCF_000741165.1	s__Bifidobacterium biavatii	86.7392	611	970	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	99.97	99.97	0.98	0.98	2	-
GCF_018555385.1	s__Bifidobacterium sp018555385	84.2565	543	970	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003952945.1	s__Bifidobacterium samirii	84.2084	524	970	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001042635.1	s__Bifidobacterium scardovii	84.1495	565	970	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	99.51	98.97	0.97	0.93	7	-
GCF_001417815.1	s__Bifidobacterium aesculapii	83.4867	469	970	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:34:41,774] [INFO] GTDB search result was written to GCF_010667685.1_ASM1066768v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:34:41,775] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:34:41,778] [INFO] DFAST_QC result json was written to GCF_010667685.1_ASM1066768v1_genomic.fna/dqc_result.json
[2024-01-24 13:34:41,778] [INFO] DFAST_QC completed!
[2024-01-24 13:34:41,779] [INFO] Total running time: 0h1m1s