[2024-01-24 14:46:57,296] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:46:57,298] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:46:57,298] [INFO] DQC Reference Directory: /var/lib/cwl/stgf8d9d289-97c9-4917-9320-ffba907ca1d6/dqc_reference
[2024-01-24 14:46:58,646] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:46:58,647] [INFO] Task started: Prodigal
[2024-01-24 14:46:58,647] [INFO] Running command: gunzip -c /var/lib/cwl/stg793cf15a-3616-4091-af5f-1a20f7d66208/GCF_010728155.1_ASM1072815v1_genomic.fna.gz | prodigal -d GCF_010728155.1_ASM1072815v1_genomic.fna/cds.fna -a GCF_010728155.1_ASM1072815v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:47:11,916] [INFO] Task succeeded: Prodigal
[2024-01-24 14:47:11,917] [INFO] Task started: HMMsearch
[2024-01-24 14:47:11,917] [INFO] Running command: hmmsearch --tblout GCF_010728155.1_ASM1072815v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf8d9d289-97c9-4917-9320-ffba907ca1d6/dqc_reference/reference_markers.hmm GCF_010728155.1_ASM1072815v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:47:12,221] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:47:12,223] [INFO] Found 6/6 markers.
[2024-01-24 14:47:12,277] [INFO] Query marker FASTA was written to GCF_010728155.1_ASM1072815v1_genomic.fna/markers.fasta
[2024-01-24 14:47:12,278] [INFO] Task started: Blastn
[2024-01-24 14:47:12,278] [INFO] Running command: blastn -query GCF_010728155.1_ASM1072815v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf8d9d289-97c9-4917-9320-ffba907ca1d6/dqc_reference/reference_markers.fasta -out GCF_010728155.1_ASM1072815v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:47:13,545] [INFO] Task succeeded: Blastn
[2024-01-24 14:47:13,548] [INFO] Selected 13 target genomes.
[2024-01-24 14:47:13,548] [INFO] Target genome list was writen to GCF_010728155.1_ASM1072815v1_genomic.fna/target_genomes.txt
[2024-01-24 14:47:13,555] [INFO] Task started: fastANI
[2024-01-24 14:47:13,555] [INFO] Running command: fastANI --query /var/lib/cwl/stg793cf15a-3616-4091-af5f-1a20f7d66208/GCF_010728155.1_ASM1072815v1_genomic.fna.gz --refList GCF_010728155.1_ASM1072815v1_genomic.fna/target_genomes.txt --output GCF_010728155.1_ASM1072815v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:47:29,431] [INFO] Task succeeded: fastANI
[2024-01-24 14:47:29,432] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf8d9d289-97c9-4917-9320-ffba907ca1d6/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:47:29,432] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf8d9d289-97c9-4917-9320-ffba907ca1d6/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:47:29,444] [INFO] Found 13 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 14:47:29,444] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:47:29,444] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Mycobacterium doricum	strain=JCM 12405	GCA_010728155.1	126673	126673	type	True	100.0	1343	1343	95	conclusive
Mycobacterium doricum	strain=DSM 44339	GCA_002102115.1	126673	126673	type	True	99.9423	1277	1343	95	conclusive
Mycolicibacterium monacense	strain=DSM 44395	GCA_002086655.1	85693	85693	type	True	87.9235	1012	1343	95	below_threshold
Mycolicibacterium monacense	strain=DSM 44395	GCA_009931355.1	85693	85693	type	True	87.9118	1026	1343	95	below_threshold
Mycolicibacterium monacense	strain=JCM 15658	GCA_010731575.1	85693	85693	type	True	87.9059	1037	1343	95	below_threshold
Mycolicibacterium litorale	strain=CGMCC 4.5724	GCA_004366555.1	758802	758802	type	True	85.4012	978	1343	95	below_threshold
Mycolicibacterium litorale	strain=JCM 17423	GCA_010731695.1	758802	758802	type	True	85.3736	992	1343	95	below_threshold
Mycolicibacterium baixiangningiae	strain=LJ126	GCA_016313185.1	2761578	2761578	type	True	85.1834	973	1343	95	below_threshold
Mycolicibacterium baixiangningiae	strain=LJ126	GCA_014893035.1	2761578	2761578	type	True	85.1518	979	1343	95	below_threshold
Mycolicibacterium komanii	strain=GPK 1020	GCA_001050035.1	1566885	1566885	type	True	80.4608	707	1343	95	below_threshold
Mycolicibacterium malmesburyense	strain=WCM 7299	GCA_001050015.1	1566886	1566886	type	True	80.4171	725	1343	95	below_threshold
Mycolicibacterium alvei	strain=JCM 12272	GCA_010727325.1	67081	67081	type	True	79.9067	646	1343	95	below_threshold
Mycolicibacterium mageritense	strain=CIP 104973	GCA_000612825.1	53462	53462	type	True	79.8739	734	1343	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:47:29,446] [INFO] DFAST Taxonomy check result was written to GCF_010728155.1_ASM1072815v1_genomic.fna/tc_result.tsv
[2024-01-24 14:47:29,447] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:47:29,447] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:47:29,447] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf8d9d289-97c9-4917-9320-ffba907ca1d6/dqc_reference/checkm_data
[2024-01-24 14:47:29,449] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:47:29,489] [INFO] Task started: CheckM
[2024-01-24 14:47:29,490] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_010728155.1_ASM1072815v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_010728155.1_ASM1072815v1_genomic.fna/checkm_input GCF_010728155.1_ASM1072815v1_genomic.fna/checkm_result
[2024-01-24 14:48:14,142] [INFO] Task succeeded: CheckM
[2024-01-24 14:48:14,143] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:48:14,163] [INFO] ===== Completeness check finished =====
[2024-01-24 14:48:14,163] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:48:14,164] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_010728155.1_ASM1072815v1_genomic.fna/markers.fasta)
[2024-01-24 14:48:14,164] [INFO] Task started: Blastn
[2024-01-24 14:48:14,164] [INFO] Running command: blastn -query GCF_010728155.1_ASM1072815v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf8d9d289-97c9-4917-9320-ffba907ca1d6/dqc_reference/reference_markers_gtdb.fasta -out GCF_010728155.1_ASM1072815v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:48:15,862] [INFO] Task succeeded: Blastn
[2024-01-24 14:48:15,867] [INFO] Selected 12 target genomes.
[2024-01-24 14:48:15,867] [INFO] Target genome list was writen to GCF_010728155.1_ASM1072815v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:48:15,896] [INFO] Task started: fastANI
[2024-01-24 14:48:15,896] [INFO] Running command: fastANI --query /var/lib/cwl/stg793cf15a-3616-4091-af5f-1a20f7d66208/GCF_010728155.1_ASM1072815v1_genomic.fna.gz --refList GCF_010728155.1_ASM1072815v1_genomic.fna/target_genomes_gtdb.txt --output GCF_010728155.1_ASM1072815v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:48:30,504] [INFO] Task succeeded: fastANI
[2024-01-24 14:48:30,520] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:48:30,520] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_010728155.1	s__Mycobacterium doricum	100.0	1343	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	100.00	100.00	1.00	1.00	2	conclusive
GCF_001499995.1	s__Mycobacterium sp001499995	88.0047	965	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_010731575.1	s__Mycobacterium monacense	87.8997	1037	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.29	98.82	0.95	0.89	9	-
GCF_001500125.1	s__Mycobacterium sp001500125	87.4216	972	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_010731695.1	s__Mycobacterium litorale	85.3567	993	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	100.00	100.00	1.00	1.00	2	-
GCF_014218295.1	s__Mycobacterium litorale_B	85.277	966	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016313185.1	s__Mycobacterium sp016313185	85.178	974	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	98.68	97.36	0.93	0.85	3	-
GCF_001050035.1	s__Mycobacterium komanii	80.4527	708	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001050015.1	s__Mycobacterium malmesburyense	80.4289	723	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_010726645.1	s__Mycobacterium duvalii	80.3672	713	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	100.00	100.00	1.00	1.00	2	-
GCF_010723735.1	s__Mycobacterium hippocampi_B	80.2943	735	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_010727325.1	s__Mycobacterium alvei	79.8979	647	1343	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 14:48:30,522] [INFO] GTDB search result was written to GCF_010728155.1_ASM1072815v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:48:30,522] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:48:30,528] [INFO] DFAST_QC result json was written to GCF_010728155.1_ASM1072815v1_genomic.fna/dqc_result.json
[2024-01-24 14:48:30,528] [INFO] DFAST_QC completed!
[2024-01-24 14:48:30,528] [INFO] Total running time: 0h1m33s