[2024-01-24 12:28:34,759] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:28:34,761] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:28:34,761] [INFO] DQC Reference Directory: /var/lib/cwl/stg124f6177-41d7-4ae0-bfb8-8552415d49ab/dqc_reference
[2024-01-24 12:28:36,000] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:28:36,001] [INFO] Task started: Prodigal
[2024-01-24 12:28:36,001] [INFO] Running command: gunzip -c /var/lib/cwl/stg076591d3-4b24-4140-9670-70be6ad40b7a/GCF_011170085.1_ASM1117008v1_genomic.fna.gz | prodigal -d GCF_011170085.1_ASM1117008v1_genomic.fna/cds.fna -a GCF_011170085.1_ASM1117008v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:28:41,207] [INFO] Task succeeded: Prodigal
[2024-01-24 12:28:41,207] [INFO] Task started: HMMsearch
[2024-01-24 12:28:41,207] [INFO] Running command: hmmsearch --tblout GCF_011170085.1_ASM1117008v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg124f6177-41d7-4ae0-bfb8-8552415d49ab/dqc_reference/reference_markers.hmm GCF_011170085.1_ASM1117008v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:28:41,495] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:28:41,497] [INFO] Found 6/6 markers.
[2024-01-24 12:28:41,520] [INFO] Query marker FASTA was written to GCF_011170085.1_ASM1117008v1_genomic.fna/markers.fasta
[2024-01-24 12:28:41,520] [INFO] Task started: Blastn
[2024-01-24 12:28:41,520] [INFO] Running command: blastn -query GCF_011170085.1_ASM1117008v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg124f6177-41d7-4ae0-bfb8-8552415d49ab/dqc_reference/reference_markers.fasta -out GCF_011170085.1_ASM1117008v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:28:42,107] [INFO] Task succeeded: Blastn
[2024-01-24 12:28:42,112] [INFO] Selected 19 target genomes.
[2024-01-24 12:28:42,112] [INFO] Target genome list was writen to GCF_011170085.1_ASM1117008v1_genomic.fna/target_genomes.txt
[2024-01-24 12:28:42,144] [INFO] Task started: fastANI
[2024-01-24 12:28:42,144] [INFO] Running command: fastANI --query /var/lib/cwl/stg076591d3-4b24-4140-9670-70be6ad40b7a/GCF_011170085.1_ASM1117008v1_genomic.fna.gz --refList GCF_011170085.1_ASM1117008v1_genomic.fna/target_genomes.txt --output GCF_011170085.1_ASM1117008v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:28:49,808] [INFO] Task succeeded: fastANI
[2024-01-24 12:28:49,808] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg124f6177-41d7-4ae0-bfb8-8552415d49ab/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:28:49,809] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg124f6177-41d7-4ae0-bfb8-8552415d49ab/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:28:49,822] [INFO] Found 11 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 12:28:49,822] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:28:49,823] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Lactococcus hodotermopsidis	strain=Hs30E4-3	GCA_011170085.1	2709157	2709157	type	True	100.0	693	696	95	conclusive
Lactococcus insecticola	strain=Hs20B0-1	GCA_011170065.1	2709158	2709158	type	True	79.1398	198	696	95	below_threshold
Lactococcus reticulitermitis	strain=Rs-Y01	GCA_002260845.1	2025039	2025039	type	True	78.7071	222	696	95	below_threshold
Lactococcus laudensis	strain=DSM 28961	GCA_013449735.1	1494461	1494461	type	True	78.1416	209	696	95	below_threshold
Lactococcus chungangensis	strain=DSM 22330	GCA_900119835.1	451457	451457	type	True	78.1372	185	696	95	below_threshold
Lactococcus laudensis	strain=LMG 28353	GCA_019586025.1	1494461	1494461	type	True	78.0666	204	696	95	below_threshold
Lactococcus raffinolactis	strain=NBRC 100932	GCA_001591765.1	1366	1366	type	True	78.0593	202	696	95	below_threshold
Lactococcus raffinolactis	strain=ATCC 43920	GCA_002441745.1	1366	1366	type	True	78.0519	200	696	95	below_threshold
Lactococcus carnosus	strain=TMW 2.1612	GCA_006770265.1	2749961	2749961	type	True	78.0299	138	696	95	below_threshold
Lactococcus chungangensis	strain=DSM 22330	GCA_002441885.1	451457	451457	type	True	78.0041	185	696	95	below_threshold
Lactococcus paracarnosus	strain=TMW 2.1615	GCA_006770285.1	2749962	2749962	type	True	77.7672	143	696	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:28:49,824] [INFO] DFAST Taxonomy check result was written to GCF_011170085.1_ASM1117008v1_genomic.fna/tc_result.tsv
[2024-01-24 12:28:49,825] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:28:49,825] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:28:49,825] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg124f6177-41d7-4ae0-bfb8-8552415d49ab/dqc_reference/checkm_data
[2024-01-24 12:28:49,826] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:28:49,851] [INFO] Task started: CheckM
[2024-01-24 12:28:49,851] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_011170085.1_ASM1117008v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_011170085.1_ASM1117008v1_genomic.fna/checkm_input GCF_011170085.1_ASM1117008v1_genomic.fna/checkm_result
[2024-01-24 12:29:12,679] [INFO] Task succeeded: CheckM
[2024-01-24 12:29:12,680] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:29:12,703] [INFO] ===== Completeness check finished =====
[2024-01-24 12:29:12,703] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:29:12,704] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_011170085.1_ASM1117008v1_genomic.fna/markers.fasta)
[2024-01-24 12:29:12,704] [INFO] Task started: Blastn
[2024-01-24 12:29:12,704] [INFO] Running command: blastn -query GCF_011170085.1_ASM1117008v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg124f6177-41d7-4ae0-bfb8-8552415d49ab/dqc_reference/reference_markers_gtdb.fasta -out GCF_011170085.1_ASM1117008v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:29:13,447] [INFO] Task succeeded: Blastn
[2024-01-24 12:29:13,451] [INFO] Selected 19 target genomes.
[2024-01-24 12:29:13,451] [INFO] Target genome list was writen to GCF_011170085.1_ASM1117008v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:29:13,496] [INFO] Task started: fastANI
[2024-01-24 12:29:13,496] [INFO] Running command: fastANI --query /var/lib/cwl/stg076591d3-4b24-4140-9670-70be6ad40b7a/GCF_011170085.1_ASM1117008v1_genomic.fna.gz --refList GCF_011170085.1_ASM1117008v1_genomic.fna/target_genomes_gtdb.txt --output GCF_011170085.1_ASM1117008v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:29:20,851] [INFO] Task succeeded: fastANI
[2024-01-24 12:29:20,863] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:29:20,863] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_011170085.1	s__Lactococcus_A hodotermopsidis	100.0	693	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_011170065.1	s__Lactococcus_A insecticola	79.1586	197	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002260845.1	s__Lactococcus_A reticulitermitis	78.7256	221	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013449735.1	s__Lactococcus_A laudensis	78.1416	209	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	97.98	97.98	0.86	0.86	2	-
GCF_001591765.1	s__Lactococcus_A raffinolactis	78.0421	203	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	99.11	98.57	0.92	0.88	14	-
GCF_006770265.1	s__Lactococcus_A carnosus	78.0299	138	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	97.28	95.96	0.91	0.89	5	-
GCF_002441885.1	s__Lactococcus_A chungangensis	78.003	185	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	99.24	98.46	0.92	0.88	4	-
GCF_002441695.1	s__Lactococcus_A piscium	77.9808	134	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_006770285.1	s__Lactococcus_A paracarnosus	77.7043	142	696	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	99.90	99.90	0.97	0.97	2	-
--------------------------------------------------------------------------------
[2024-01-24 12:29:20,865] [INFO] GTDB search result was written to GCF_011170085.1_ASM1117008v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:29:20,865] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:29:20,870] [INFO] DFAST_QC result json was written to GCF_011170085.1_ASM1117008v1_genomic.fna/dqc_result.json
[2024-01-24 12:29:20,870] [INFO] DFAST_QC completed!
[2024-01-24 12:29:20,870] [INFO] Total running time: 0h0m46s