[2024-01-24 11:19:54,413] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:19:54,418] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:19:54,418] [INFO] DQC Reference Directory: /var/lib/cwl/stg51e19ef9-f4f6-4103-9f43-d58e0bf86ecb/dqc_reference
[2024-01-24 11:19:55,815] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:19:55,816] [INFO] Task started: Prodigal
[2024-01-24 11:19:55,816] [INFO] Running command: gunzip -c /var/lib/cwl/stga4c1c545-37ce-46a9-a390-1ce6b0b8ac71/GCF_011801475.1_ASM1180147v1_genomic.fna.gz | prodigal -d GCF_011801475.1_ASM1180147v1_genomic.fna/cds.fna -a GCF_011801475.1_ASM1180147v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:20:04,015] [INFO] Task succeeded: Prodigal
[2024-01-24 11:20:04,016] [INFO] Task started: HMMsearch
[2024-01-24 11:20:04,016] [INFO] Running command: hmmsearch --tblout GCF_011801475.1_ASM1180147v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg51e19ef9-f4f6-4103-9f43-d58e0bf86ecb/dqc_reference/reference_markers.hmm GCF_011801475.1_ASM1180147v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:20:04,255] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:20:04,257] [INFO] Found 6/6 markers.
[2024-01-24 11:20:04,282] [INFO] Query marker FASTA was written to GCF_011801475.1_ASM1180147v1_genomic.fna/markers.fasta
[2024-01-24 11:20:04,282] [INFO] Task started: Blastn
[2024-01-24 11:20:04,282] [INFO] Running command: blastn -query GCF_011801475.1_ASM1180147v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg51e19ef9-f4f6-4103-9f43-d58e0bf86ecb/dqc_reference/reference_markers.fasta -out GCF_011801475.1_ASM1180147v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:20:04,987] [INFO] Task succeeded: Blastn
[2024-01-24 11:20:04,990] [INFO] Selected 10 target genomes.
[2024-01-24 11:20:04,990] [INFO] Target genome list was writen to GCF_011801475.1_ASM1180147v1_genomic.fna/target_genomes.txt
[2024-01-24 11:20:04,995] [INFO] Task started: fastANI
[2024-01-24 11:20:04,995] [INFO] Running command: fastANI --query /var/lib/cwl/stga4c1c545-37ce-46a9-a390-1ce6b0b8ac71/GCF_011801475.1_ASM1180147v1_genomic.fna.gz --refList GCF_011801475.1_ASM1180147v1_genomic.fna/target_genomes.txt --output GCF_011801475.1_ASM1180147v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:20:11,131] [INFO] Task succeeded: fastANI
[2024-01-24 11:20:11,131] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg51e19ef9-f4f6-4103-9f43-d58e0bf86ecb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:20:11,131] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg51e19ef9-f4f6-4103-9f43-d58e0bf86ecb/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:20:11,139] [INFO] Found 6 fastANI hits (4 hits with ANI > threshold)
[2024-01-24 11:20:11,139] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:20:11,139] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Xylella fastidiosa	strain=ATCC 35879	GCA_011801475.1	2371	2371	type	True	100.0	864	868	95	conclusive
Xylella fastidiosa	strain=ATCC 35879	GCA_000767565.1	2371	2371	type	True	99.9563	818	868	95	conclusive
Xylella fastidiosa	strain=DSM 10026	GCA_900129695.1	2371	2371	type	True	99.9096	770	868	95	conclusive
Xylella fastidiosa subsp. multiplex	strain=ATCC 35871	GCA_000428665.1	644357	2371	type	True	97.9963	753	868	95	conclusive
Xylella taiwanensis	strain=PLS229	GCA_013177435.1	1444770	1444770	type	True	82.7712	665	868	95	below_threshold
Xanthomonas translucens	strain=ATCC 19319	GCA_020880735.1	343	343	type	True	76.514	56	868	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:20:11,141] [INFO] DFAST Taxonomy check result was written to GCF_011801475.1_ASM1180147v1_genomic.fna/tc_result.tsv
[2024-01-24 11:20:11,142] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:20:11,142] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:20:11,142] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg51e19ef9-f4f6-4103-9f43-d58e0bf86ecb/dqc_reference/checkm_data
[2024-01-24 11:20:11,144] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:20:11,177] [INFO] Task started: CheckM
[2024-01-24 11:20:11,178] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_011801475.1_ASM1180147v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_011801475.1_ASM1180147v1_genomic.fna/checkm_input GCF_011801475.1_ASM1180147v1_genomic.fna/checkm_result
[2024-01-24 11:20:41,542] [INFO] Task succeeded: CheckM
[2024-01-24 11:20:41,544] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:20:41,571] [INFO] ===== Completeness check finished =====
[2024-01-24 11:20:41,571] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:20:41,572] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_011801475.1_ASM1180147v1_genomic.fna/markers.fasta)
[2024-01-24 11:20:41,572] [INFO] Task started: Blastn
[2024-01-24 11:20:41,572] [INFO] Running command: blastn -query GCF_011801475.1_ASM1180147v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg51e19ef9-f4f6-4103-9f43-d58e0bf86ecb/dqc_reference/reference_markers_gtdb.fasta -out GCF_011801475.1_ASM1180147v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:20:42,758] [INFO] Task succeeded: Blastn
[2024-01-24 11:20:42,763] [INFO] Selected 23 target genomes.
[2024-01-24 11:20:42,763] [INFO] Target genome list was writen to GCF_011801475.1_ASM1180147v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:20:42,785] [INFO] Task started: fastANI
[2024-01-24 11:20:42,786] [INFO] Running command: fastANI --query /var/lib/cwl/stga4c1c545-37ce-46a9-a390-1ce6b0b8ac71/GCF_011801475.1_ASM1180147v1_genomic.fna.gz --refList GCF_011801475.1_ASM1180147v1_genomic.fna/target_genomes_gtdb.txt --output GCF_011801475.1_ASM1180147v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:20:57,293] [INFO] Task succeeded: fastANI
[2024-01-24 11:20:57,310] [INFO] Found 14 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:20:57,311] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_011801475.1	s__Xylella fastidiosa	100.0	865	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xylella	95.0	98.40	96.23	0.96	0.89	130	conclusive
GCF_013177435.1	s__Xylella taiwanensis	82.7828	663	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xylella	95.0	100.00	99.99	1.00	0.99	4	-
GCF_000007145.1	s__Xanthomonas campestris	77.1507	64	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xanthomonas	95.0	98.22	96.56	0.94	0.87	68	-
GCF_013387485.1	s__Stenotrophomonas maltophilia_AA	76.9349	56	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Stenotrophomonas	95.0	97.19	96.54	0.90	0.89	3	-
GCF_001908725.1	s__Xanthomonas vesicatoria	76.8774	70	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xanthomonas	95.0	99.30	98.65	0.97	0.94	15	-
GCF_004216795.1	s__Stenotrophomonas_A sp004216795	76.8271	50	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Stenotrophomonas_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004348115.1	s__Stenotrophomonas sp004348115	76.8259	53	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Stenotrophomonas	95.0	98.00	98.00	0.94	0.94	2	-
GCF_001431465.1	s__Stenotrophomonas terrae	76.6952	56	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Stenotrophomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001423585.1	s__Xanthomonas sp001423585	76.6906	68	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xanthomonas	95.0	98.11	97.14	0.95	0.93	10	-
GCF_900215535.1	s__Pseudoxanthomonas_A wuyuanensis	76.6563	50	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Pseudoxanthomonas_A	95.0	99.99	99.99	0.99	0.99	2	-
GCF_000331775.1	s__Xanthomonas_A translucens	76.5536	55	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xanthomonas_A	95.0	97.09	95.18	0.88	0.77	59	-
GCF_002939865.1	s__Xanthomonas dyei	76.3358	63	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xanthomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002940205.1	s__Xanthomonas arboricola_C	76.2756	67	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xanthomonas	95.0	99.99	99.99	0.99	0.99	2	-
GCF_900183975.1	s__Xanthomonas fragariae	76.1682	61	868	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Xanthomonas	95.0	99.74	99.53	0.99	0.96	62	-
--------------------------------------------------------------------------------
[2024-01-24 11:20:57,312] [INFO] GTDB search result was written to GCF_011801475.1_ASM1180147v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:20:57,313] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:20:57,317] [INFO] DFAST_QC result json was written to GCF_011801475.1_ASM1180147v1_genomic.fna/dqc_result.json
[2024-01-24 11:20:57,318] [INFO] DFAST_QC completed!
[2024-01-24 11:20:57,318] [INFO] Total running time: 0h1m3s