[2024-01-24 12:14:35,598] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:14:35,600] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:14:35,600] [INFO] DQC Reference Directory: /var/lib/cwl/stgc7965199-72b0-44e8-9d37-1b67f345ad95/dqc_reference
[2024-01-24 12:14:36,906] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:14:36,907] [INFO] Task started: Prodigal
[2024-01-24 12:14:36,907] [INFO] Running command: gunzip -c /var/lib/cwl/stg52338cc0-6a35-4cfb-9bfb-3d07839a1738/GCF_013373895.1_ASM1337389v1_genomic.fna.gz | prodigal -d GCF_013373895.1_ASM1337389v1_genomic.fna/cds.fna -a GCF_013373895.1_ASM1337389v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:14:57,728] [INFO] Task succeeded: Prodigal
[2024-01-24 12:14:57,728] [INFO] Task started: HMMsearch
[2024-01-24 12:14:57,729] [INFO] Running command: hmmsearch --tblout GCF_013373895.1_ASM1337389v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc7965199-72b0-44e8-9d37-1b67f345ad95/dqc_reference/reference_markers.hmm GCF_013373895.1_ASM1337389v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:14:58,065] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:14:58,066] [INFO] Found 6/6 markers.
[2024-01-24 12:14:58,115] [INFO] Query marker FASTA was written to GCF_013373895.1_ASM1337389v1_genomic.fna/markers.fasta
[2024-01-24 12:14:58,115] [INFO] Task started: Blastn
[2024-01-24 12:14:58,115] [INFO] Running command: blastn -query GCF_013373895.1_ASM1337389v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc7965199-72b0-44e8-9d37-1b67f345ad95/dqc_reference/reference_markers.fasta -out GCF_013373895.1_ASM1337389v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:14:59,090] [INFO] Task succeeded: Blastn
[2024-01-24 12:14:59,093] [INFO] Selected 18 target genomes.
[2024-01-24 12:14:59,093] [INFO] Target genome list was writen to GCF_013373895.1_ASM1337389v1_genomic.fna/target_genomes.txt
[2024-01-24 12:14:59,098] [INFO] Task started: fastANI
[2024-01-24 12:14:59,098] [INFO] Running command: fastANI --query /var/lib/cwl/stg52338cc0-6a35-4cfb-9bfb-3d07839a1738/GCF_013373895.1_ASM1337389v1_genomic.fna.gz --refList GCF_013373895.1_ASM1337389v1_genomic.fna/target_genomes.txt --output GCF_013373895.1_ASM1337389v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:15:22,549] [INFO] Task succeeded: fastANI
[2024-01-24 12:15:22,550] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc7965199-72b0-44e8-9d37-1b67f345ad95/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:15:22,550] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc7965199-72b0-44e8-9d37-1b67f345ad95/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:15:22,565] [INFO] Found 18 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 12:15:22,565] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:15:22,566] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Pseudomonas ceruminis	strain=BML-PP028	GCA_013373895.1	2740516	2740516	type	True	100.0	2037	2042	95	conclusive
Pseudomonas ceruminis	strain=BML-PP028	GCA_021602025.1	2740516	2740516	type	True	99.9983	2038	2042	95	conclusive
Pseudomonas faucium	strain=BML-PP048	GCA_021602585.1	2740518	2740518	type	True	88.2645	1397	2042	95	below_threshold
Pseudomonas faucium	strain=BML-PP048	GCA_013373935.1	2740518	2740518	type	True	88.2561	1309	2042	95	below_threshold
Pseudomonas plecoglossicida	strain=NBRC 103162	GCA_000730665.1	70775	70775	suspected-type	True	87.6237	1287	2042	95	below_threshold
Pseudomonas plecoglossicida	strain=DSM 15088	GCA_000688275.1	70775	70775	suspected-type	True	87.5947	1312	2042	95	below_threshold
Pseudomonas urethralis	strain=BML-PP042	GCA_013373915.1	2740517	2740517	suspected-type	True	87.0676	1344	2042	95	below_threshold
Pseudomonas urethralis	strain=BML-PP042	GCA_021602465.1	2740517	2740517	suspected-type	True	87.0521	1377	2042	95	below_threshold
Pseudomonas capeferrum	strain=WCS358	GCA_022548835.1	1495066	1495066	type	True	86.8054	1245	2042	95	below_threshold
Pseudomonas capeferrum	strain=WCS358	GCA_000731675.1	1495066	1495066	type	True	86.7851	1363	2042	95	below_threshold
Pseudomonas anuradhapurensis	strain=RD8MR3	GCA_014269225.2	485870	485870	type	True	86.633	1272	2042	95	below_threshold
Pseudomonas kermanshahensis	strain=SWRI100	GCA_014269205.2	2745482	2745482	type	True	86.5306	1351	2042	95	below_threshold
Pseudomonas oryzicola	strain=RD9SR1	GCA_014269185.2	485876	485876	type	True	86.3771	1279	2042	95	below_threshold
Pseudomonas muyukensis	strain=COW39	GCA_019139535.1	2842357	2842357	type	True	85.9383	1253	2042	95	below_threshold
Pseudomonas defluvii	strain=WCHP16	GCA_001695625.1	1876757	1876757	type	True	82.1526	832	2042	95	below_threshold
Pseudomonas delhiensis	strain=RLD-1	GCA_900187975.1	366289	366289	type	True	80.7624	905	2042	95	below_threshold
Pseudomonas delhiensis	strain=CCM 7361	GCA_900099945.1	366289	366289	type	True	80.7429	893	2042	95	below_threshold
Pseudomonas rhizoryzae	strain=RY24	GCA_005250615.1	2571129	2571129	type	True	79.1717	540	2042	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:15:22,568] [INFO] DFAST Taxonomy check result was written to GCF_013373895.1_ASM1337389v1_genomic.fna/tc_result.tsv
[2024-01-24 12:15:22,569] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:15:22,569] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:15:22,569] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc7965199-72b0-44e8-9d37-1b67f345ad95/dqc_reference/checkm_data
[2024-01-24 12:15:22,570] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:15:22,636] [INFO] Task started: CheckM
[2024-01-24 12:15:22,637] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_013373895.1_ASM1337389v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_013373895.1_ASM1337389v1_genomic.fna/checkm_input GCF_013373895.1_ASM1337389v1_genomic.fna/checkm_result
[2024-01-24 12:16:22,334] [INFO] Task succeeded: CheckM
[2024-01-24 12:16:22,335] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:16:22,356] [INFO] ===== Completeness check finished =====
[2024-01-24 12:16:22,356] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:16:22,357] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_013373895.1_ASM1337389v1_genomic.fna/markers.fasta)
[2024-01-24 12:16:22,357] [INFO] Task started: Blastn
[2024-01-24 12:16:22,357] [INFO] Running command: blastn -query GCF_013373895.1_ASM1337389v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc7965199-72b0-44e8-9d37-1b67f345ad95/dqc_reference/reference_markers_gtdb.fasta -out GCF_013373895.1_ASM1337389v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:16:23,992] [INFO] Task succeeded: Blastn
[2024-01-24 12:16:23,995] [INFO] Selected 22 target genomes.
[2024-01-24 12:16:23,995] [INFO] Target genome list was writen to GCF_013373895.1_ASM1337389v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:16:24,012] [INFO] Task started: fastANI
[2024-01-24 12:16:24,013] [INFO] Running command: fastANI --query /var/lib/cwl/stg52338cc0-6a35-4cfb-9bfb-3d07839a1738/GCF_013373895.1_ASM1337389v1_genomic.fna.gz --refList GCF_013373895.1_ASM1337389v1_genomic.fna/target_genomes_gtdb.txt --output GCF_013373895.1_ASM1337389v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:16:53,748] [INFO] Task succeeded: fastANI
[2024-01-24 12:16:53,767] [INFO] Found 22 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:16:53,767] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001320525.1	s__Pseudomonas_E sp001320525	98.7757	1777	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	99.10	98.78	0.89	0.86	10	conclusive
GCF_013373935.1	s__Pseudomonas_E sp002438125	88.2772	1307	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	98.14	98.14	0.84	0.84	2	-
GCF_000282535.1	s__Pseudomonas_E sp000282535	87.7656	1247	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000730665.1	s__Pseudomonas_E plecoglossicida	87.6338	1286	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	99.81	99.74	0.97	0.95	6	-
GCF_002112505.1	s__Pseudomonas_E sp002112505	87.5578	1400	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	100.00	100.00	1.00	0.99	6	-
GCA_003428805.1	s__Pseudomonas_E sp003428805	87.4844	1467	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	98.29	96.05	0.90	0.83	9	-
GCF_000019445.1	s__Pseudomonas_E putida_E	87.4571	1383	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	96.71	96.19	0.87	0.82	12	-
GCF_000935045.1	s__Pseudomonas_E sp000935045	87.0436	1345	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002165135.1	s__Pseudomonas_E sp002165135	86.7857	1274	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000731675.1	s__Pseudomonas_E capeferrum	86.7778	1364	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	99.03	98.55	0.93	0.90	7	-
GCF_002736125.1	s__Pseudomonas_E putida_M	86.6298	1374	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	97.41	97.25	0.91	0.90	8	-
GCF_000730605.1	s__Pseudomonas_E monteilii	86.4725	1381	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	97.80	95.46	0.86	0.79	45	-
GCF_016648105.1	s__Pseudomonas_E sp016648105	86.4017	1249	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002356095.1	s__Pseudomonas_E putida_H	86.1421	1475	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	98.82	98.35	0.91	0.82	20	-
GCF_003671955.1	s__Pseudomonas_E hunanensis	85.9941	1312	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	97.33	95.26	0.86	0.79	88	-
GCF_019139535.1	s__Pseudomonas_E sp019139535	85.9519	1252	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004519305.1	s__Pseudomonas_E sp004519305	85.8089	1193	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009932375.1	s__Pseudomonas_E juntendi	85.7585	1201	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	98.06	97.13	0.84	0.79	41	-
GCF_002741075.1	s__Pseudomonas_E putida_T	85.4266	1244	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	98.21	95.06	0.91	0.90	4	-
GCF_004357765.1	s__Pseudomonas_E putida_U	85.3386	1211	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	99.43	99.43	0.90	0.90	2	-
GCF_013523055.1	s__Pseudomonas_E capeferrum_A	83.2703	1111	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002806685.1	s__Pseudomonas_E qingdaonensis	83.246	1156	2042	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	99.05	98.91	0.93	0.89	13	-
--------------------------------------------------------------------------------
[2024-01-24 12:16:53,770] [INFO] GTDB search result was written to GCF_013373895.1_ASM1337389v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:16:53,771] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:16:53,777] [INFO] DFAST_QC result json was written to GCF_013373895.1_ASM1337389v1_genomic.fna/dqc_result.json
[2024-01-24 12:16:53,777] [INFO] DFAST_QC completed!
[2024-01-24 12:16:53,777] [INFO] Total running time: 0h2m18s