[2024-01-25 20:15:05,801] [INFO] DFAST_QC pipeline started.
[2024-01-25 20:15:05,804] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 20:15:05,804] [INFO] DQC Reference Directory: /var/lib/cwl/stg4dfc29b4-df1d-4352-bcb4-0bc790f8fc41/dqc_reference
[2024-01-25 20:15:06,930] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 20:15:06,933] [INFO] Task started: Prodigal
[2024-01-25 20:15:06,934] [INFO] Running command: gunzip -c /var/lib/cwl/stg7365ffb3-4cba-42d0-9154-45b6a7a11257/GCF_013393365.1_ASM1339336v1_genomic.fna.gz | prodigal -d GCF_013393365.1_ASM1339336v1_genomic.fna/cds.fna -a GCF_013393365.1_ASM1339336v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 20:15:10,952] [INFO] Task succeeded: Prodigal
[2024-01-25 20:15:10,952] [INFO] Task started: HMMsearch
[2024-01-25 20:15:10,953] [INFO] Running command: hmmsearch --tblout GCF_013393365.1_ASM1339336v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg4dfc29b4-df1d-4352-bcb4-0bc790f8fc41/dqc_reference/reference_markers.hmm GCF_013393365.1_ASM1339336v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 20:15:11,203] [INFO] Task succeeded: HMMsearch
[2024-01-25 20:15:11,204] [INFO] Found 6/6 markers.
[2024-01-25 20:15:11,225] [INFO] Query marker FASTA was written to GCF_013393365.1_ASM1339336v1_genomic.fna/markers.fasta
[2024-01-25 20:15:11,225] [INFO] Task started: Blastn
[2024-01-25 20:15:11,225] [INFO] Running command: blastn -query GCF_013393365.1_ASM1339336v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg4dfc29b4-df1d-4352-bcb4-0bc790f8fc41/dqc_reference/reference_markers.fasta -out GCF_013393365.1_ASM1339336v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:15:11,773] [INFO] Task succeeded: Blastn
[2024-01-25 20:15:11,776] [INFO] Selected 6 target genomes.
[2024-01-25 20:15:11,776] [INFO] Target genome list was writen to GCF_013393365.1_ASM1339336v1_genomic.fna/target_genomes.txt
[2024-01-25 20:15:11,780] [INFO] Task started: fastANI
[2024-01-25 20:15:11,780] [INFO] Running command: fastANI --query /var/lib/cwl/stg7365ffb3-4cba-42d0-9154-45b6a7a11257/GCF_013393365.1_ASM1339336v1_genomic.fna.gz --refList GCF_013393365.1_ASM1339336v1_genomic.fna/target_genomes.txt --output GCF_013393365.1_ASM1339336v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 20:15:14,890] [INFO] Task succeeded: fastANI
[2024-01-25 20:15:14,890] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg4dfc29b4-df1d-4352-bcb4-0bc790f8fc41/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 20:15:14,890] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg4dfc29b4-df1d-4352-bcb4-0bc790f8fc41/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 20:15:14,903] [INFO] Found 6 fastANI hits (1 hits with ANI > threshold)
[2024-01-25 20:15:14,903] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 20:15:14,903] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Veillonella nakazawae	strain=T1-7	GCA_013393365.1	2682456	2682456	type	True	100.0	699	699	95	conclusive
Veillonella infantium	strain=T11011-4	GCA_002959895.1	1911679	1911679	type	True	94.786	625	699	95	below_threshold
Veillonella dispar	strain=NCTC11831	GCA_900637515.1	39778	39778	suspected-type	True	93.5269	631	699	95	below_threshold
Veillonella dispar	strain=ATCC 17748	GCA_000160015.1	39778	39778	suspected-type	True	93.4688	632	699	95	below_threshold
Veillonella rogosae	strain=JCM 15642	GCA_002959775.1	423477	423477	type	True	90.1949	613	699	95	below_threshold
Veillonella rogosae	strain=JCM 15642	GCA_001312485.1	423477	423477	type	True	90.0367	585	699	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 20:15:14,904] [INFO] DFAST Taxonomy check result was written to GCF_013393365.1_ASM1339336v1_genomic.fna/tc_result.tsv
[2024-01-25 20:15:14,905] [INFO] ===== Taxonomy check completed =====
[2024-01-25 20:15:14,905] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 20:15:14,905] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg4dfc29b4-df1d-4352-bcb4-0bc790f8fc41/dqc_reference/checkm_data
[2024-01-25 20:15:14,906] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 20:15:14,930] [INFO] Task started: CheckM
[2024-01-25 20:15:14,930] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_013393365.1_ASM1339336v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_013393365.1_ASM1339336v1_genomic.fna/checkm_input GCF_013393365.1_ASM1339336v1_genomic.fna/checkm_result
[2024-01-25 20:15:32,402] [INFO] Task succeeded: CheckM
[2024-01-25 20:15:32,403] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 20:15:32,420] [INFO] ===== Completeness check finished =====
[2024-01-25 20:15:32,420] [INFO] ===== Start GTDB Search =====
[2024-01-25 20:15:32,420] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_013393365.1_ASM1339336v1_genomic.fna/markers.fasta)
[2024-01-25 20:15:32,421] [INFO] Task started: Blastn
[2024-01-25 20:15:32,421] [INFO] Running command: blastn -query GCF_013393365.1_ASM1339336v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg4dfc29b4-df1d-4352-bcb4-0bc790f8fc41/dqc_reference/reference_markers_gtdb.fasta -out GCF_013393365.1_ASM1339336v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:15:33,170] [INFO] Task succeeded: Blastn
[2024-01-25 20:15:33,173] [INFO] Selected 12 target genomes.
[2024-01-25 20:15:33,173] [INFO] Target genome list was writen to GCF_013393365.1_ASM1339336v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 20:15:33,179] [INFO] Task started: fastANI
[2024-01-25 20:15:33,179] [INFO] Running command: fastANI --query /var/lib/cwl/stg7365ffb3-4cba-42d0-9154-45b6a7a11257/GCF_013393365.1_ASM1339336v1_genomic.fna.gz --refList GCF_013393365.1_ASM1339336v1_genomic.fna/target_genomes_gtdb.txt --output GCF_013393365.1_ASM1339336v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 20:15:38,878] [INFO] Task succeeded: fastANI
[2024-01-25 20:15:38,886] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 20:15:38,886] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_013393365.1	s__Veillonella nakazawae	100.0	699	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	96.23	95.05	0.93	0.87	33	conclusive
GCF_002959895.1	s__Veillonella infantium	94.7925	624	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	95.95	95.95	0.93	0.93	2	-
GCF_003463825.1	s__Veillonella sp003463825	94.6843	569	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	97.70	95.41	0.92	0.86	3	-
GCA_900766125.1	s__Veillonella sp900766125	94.4227	326	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900637515.1	s__Veillonella dispar	93.5269	631	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	96.54	95.48	0.96	0.91	7	-
GCF_002959755.1	s__Veillonella sp002959755	93.4403	627	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900545205.1	s__Veillonella sp900545205	93.1377	508	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	97.54	95.18	0.86	0.80	3	-
GCA_900550175.1	s__Veillonella sp900550175	92.8314	431	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	98.03	98.03	0.91	0.91	2	-
GCA_905209685.1	s__Veillonella sp905209685	92.3505	478	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	95.07	95.07	0.78	0.78	2	-
GCA_902834965.1	s__Veillonella sp902834965	92.0092	288	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018367495.1	s__Veillonella sp018367495	91.6795	491	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002959775.1	s__Veillonella rogosae	90.1936	613	699	d__Bacteria;p__Firmicutes_C;c__Negativicutes;o__Veillonellales;f__Veillonellaceae;g__Veillonella	95.0	96.92	95.91	0.92	0.87	12	-
--------------------------------------------------------------------------------
[2024-01-25 20:15:38,887] [INFO] GTDB search result was written to GCF_013393365.1_ASM1339336v1_genomic.fna/result_gtdb.tsv
[2024-01-25 20:15:38,888] [INFO] ===== GTDB Search completed =====
[2024-01-25 20:15:38,890] [INFO] DFAST_QC result json was written to GCF_013393365.1_ASM1339336v1_genomic.fna/dqc_result.json
[2024-01-25 20:15:38,890] [INFO] DFAST_QC completed!
[2024-01-25 20:15:38,890] [INFO] Total running time: 0h0m33s