[2024-01-24 15:26:39,756] [INFO] DFAST_QC pipeline started.
[2024-01-24 15:26:39,759] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 15:26:39,759] [INFO] DQC Reference Directory: /var/lib/cwl/stg94f9daf4-b86b-4808-9822-89d03c58ba69/dqc_reference
[2024-01-24 15:26:41,070] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 15:26:41,070] [INFO] Task started: Prodigal
[2024-01-24 15:26:41,071] [INFO] Running command: gunzip -c /var/lib/cwl/stg224fff12-986f-4be3-bf50-c223b2178c1f/GCF_013416295.1_ASM1341629v1_genomic.fna.gz | prodigal -d GCF_013416295.1_ASM1341629v1_genomic.fna/cds.fna -a GCF_013416295.1_ASM1341629v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 15:26:54,733] [INFO] Task succeeded: Prodigal
[2024-01-24 15:26:54,733] [INFO] Task started: HMMsearch
[2024-01-24 15:26:54,734] [INFO] Running command: hmmsearch --tblout GCF_013416295.1_ASM1341629v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg94f9daf4-b86b-4808-9822-89d03c58ba69/dqc_reference/reference_markers.hmm GCF_013416295.1_ASM1341629v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 15:26:55,057] [INFO] Task succeeded: HMMsearch
[2024-01-24 15:26:55,058] [INFO] Found 6/6 markers.
[2024-01-24 15:26:55,094] [INFO] Query marker FASTA was written to GCF_013416295.1_ASM1341629v1_genomic.fna/markers.fasta
[2024-01-24 15:26:55,094] [INFO] Task started: Blastn
[2024-01-24 15:26:55,094] [INFO] Running command: blastn -query GCF_013416295.1_ASM1341629v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg94f9daf4-b86b-4808-9822-89d03c58ba69/dqc_reference/reference_markers.fasta -out GCF_013416295.1_ASM1341629v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 15:26:56,065] [INFO] Task succeeded: Blastn
[2024-01-24 15:26:56,068] [INFO] Selected 18 target genomes.
[2024-01-24 15:26:56,068] [INFO] Target genome list was writen to GCF_013416295.1_ASM1341629v1_genomic.fna/target_genomes.txt
[2024-01-24 15:26:56,075] [INFO] Task started: fastANI
[2024-01-24 15:26:56,075] [INFO] Running command: fastANI --query /var/lib/cwl/stg224fff12-986f-4be3-bf50-c223b2178c1f/GCF_013416295.1_ASM1341629v1_genomic.fna.gz --refList GCF_013416295.1_ASM1341629v1_genomic.fna/target_genomes.txt --output GCF_013416295.1_ASM1341629v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 15:27:12,646] [INFO] Task succeeded: fastANI
[2024-01-24 15:27:12,646] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg94f9daf4-b86b-4808-9822-89d03c58ba69/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 15:27:12,647] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg94f9daf4-b86b-4808-9822-89d03c58ba69/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 15:27:12,659] [INFO] Found 15 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 15:27:12,659] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 15:27:12,659] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Pusillimonas noertemannii	strain=DSM 10065	GCA_013416295.1	305977	305977	type	True	100.0	1394	1395	95	conclusive
Pusillimonas noertemannii	strain=DSM 10065	GCA_003545825.1	305977	305977	type	True	99.9994	1388	1395	95	conclusive
Pusillimonas noertemannii	strain=DSM 10065	GCA_003096595.1	305977	305977	type	True	99.9959	1384	1395	95	conclusive
Pusillimonas caeni	strain=KCTC 42353	GCA_003545815.1	1348472	1348472	type	True	86.715	1010	1395	95	below_threshold
Candidimonas nitroreducens	strain=SC-089	GCA_002209565.1	683354	683354	type	True	79.8142	470	1395	95	below_threshold
Parapusillimonas granuli	strain=LMG 24012	GCA_013416525.1	380911	380911	type	True	79.7835	466	1395	95	below_threshold
Candidimonas humi	strain=DSM 25336	GCA_019166065.1	683355	683355	type	True	79.5796	452	1395	95	below_threshold
Eoetvoesia caeni	strain=PB3-7B	GCA_022688825.1	645616	645616	type	True	79.1211	508	1395	95	below_threshold
Bordetella pertussis	strain=18323	GCA_000306945.1	520	520	type	True	79.005	428	1395	95	below_threshold
Bordetella bronchiseptica	strain=CCUG 219	GCA_021391275.1	518	518	suspected-type	True	78.9863	428	1395	95	below_threshold
Achromobacter xylosoxidans	strain=PartM-Axylosoxidans-RM8376	GCA_022870085.1	85698	85698	type	True	78.9335	460	1395	95	below_threshold
Bordetella pseudohinzii	strain=8-296-03	GCA_000657795.2	1331258	1331258	type	True	78.6711	423	1395	95	below_threshold
Achromobacter insolitus	strain=NCTC 13520	GCA_024168865.1	217204	217204	type	True	78.3819	381	1395	95	below_threshold
Ralstonia pseudosolanacearum	strain=LMG 9673	GCA_919586305.1	1310165	1310165	type	True	77.1234	240	1395	95	below_threshold
Thauera humireducens	strain=SgZ-1	GCA_001051995.2	1134435	1134435	type	True	76.5025	172	1395	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 15:27:12,661] [INFO] DFAST Taxonomy check result was written to GCF_013416295.1_ASM1341629v1_genomic.fna/tc_result.tsv
[2024-01-24 15:27:12,662] [INFO] ===== Taxonomy check completed =====
[2024-01-24 15:27:12,662] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 15:27:12,662] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg94f9daf4-b86b-4808-9822-89d03c58ba69/dqc_reference/checkm_data
[2024-01-24 15:27:12,663] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 15:27:12,708] [INFO] Task started: CheckM
[2024-01-24 15:27:12,708] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_013416295.1_ASM1341629v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_013416295.1_ASM1341629v1_genomic.fna/checkm_input GCF_013416295.1_ASM1341629v1_genomic.fna/checkm_result
[2024-01-24 15:27:54,346] [INFO] Task succeeded: CheckM
[2024-01-24 15:27:54,349] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 15:27:54,376] [INFO] ===== Completeness check finished =====
[2024-01-24 15:27:54,377] [INFO] ===== Start GTDB Search =====
[2024-01-24 15:27:54,377] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_013416295.1_ASM1341629v1_genomic.fna/markers.fasta)
[2024-01-24 15:27:54,378] [INFO] Task started: Blastn
[2024-01-24 15:27:54,378] [INFO] Running command: blastn -query GCF_013416295.1_ASM1341629v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg94f9daf4-b86b-4808-9822-89d03c58ba69/dqc_reference/reference_markers_gtdb.fasta -out GCF_013416295.1_ASM1341629v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 15:27:56,031] [INFO] Task succeeded: Blastn
[2024-01-24 15:27:56,035] [INFO] Selected 18 target genomes.
[2024-01-24 15:27:56,036] [INFO] Target genome list was writen to GCF_013416295.1_ASM1341629v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 15:27:56,050] [INFO] Task started: fastANI
[2024-01-24 15:27:56,050] [INFO] Running command: fastANI --query /var/lib/cwl/stg224fff12-986f-4be3-bf50-c223b2178c1f/GCF_013416295.1_ASM1341629v1_genomic.fna.gz --refList GCF_013416295.1_ASM1341629v1_genomic.fna/target_genomes_gtdb.txt --output GCF_013416295.1_ASM1341629v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 15:28:12,079] [INFO] Task succeeded: fastANI
[2024-01-24 15:28:12,098] [INFO] Found 17 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 15:28:12,098] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003096595.1	s__Pusillimonas noertemannii	99.9959	1384	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Pusillimonas	95.0	99.25	97.76	0.97	0.91	4	conclusive
GCF_003545815.1	s__Pusillimonas caeni	86.6828	1013	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Pusillimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002209565.1	s__Candidimonas nitroreducens	79.8398	467	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Candidimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013416525.1	s__Parapusillimonas granuli	79.7835	466	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Parapusillimonas	95.0	100.00	100.00	0.99	0.99	2	-
GCF_019166065.1	s__Candidimonas humi	79.5901	451	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Candidimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013416435.1	s__Parapusillimonas sp013416435	79.2911	396	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Parapusillimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001270295.1	s__Achromobacter sp001270295	79.2877	375	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Achromobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_017356245.1	s__Bordetella_A petrii_D	79.2871	476	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Bordetella_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002261345.1	s__Bordetella_A sp002261345	79.2398	446	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Bordetella_A	95.0	99.26	99.26	0.94	0.94	2	-
GCF_017745595.1	s__Bordetella_A petrii_C	79.2287	475	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Bordetella_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900129885.1	s__Candidimonas_A bauzanensis	79.0025	460	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Candidimonas_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002261225.1	s__Bordetella_C sp002261225	78.7137	420	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Bordetella_C	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002261265.1	s__Bordetella_A sp002261265	78.639	388	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Bordetella_A	95.0	99.22	99.22	0.95	0.94	3	-
GCF_002188635.1	s__Pigmentiphaga sp002188635	78.0373	389	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Burkholderiaceae;g__Pigmentiphaga	95.0	99.65	99.31	0.97	0.93	3	-
GCF_001051995.2	s__Thauera humireducens	76.503	172	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Rhodocyclaceae;g__Thauera	95.0	97.91	97.91	0.83	0.83	2	-
GCA_001577345.1	s__Gallionella acididurans	76.2949	60	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Gallionellaceae;g__Gallionella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003702045.1	s__Pseudomonas_E viridiflava_C	75.7493	56	1395	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Pseudomonadaceae;g__Pseudomonas_E	95.0	99.39	99.39	0.95	0.95	2	-
--------------------------------------------------------------------------------
[2024-01-24 15:28:12,099] [INFO] GTDB search result was written to GCF_013416295.1_ASM1341629v1_genomic.fna/result_gtdb.tsv
[2024-01-24 15:28:12,100] [INFO] ===== GTDB Search completed =====
[2024-01-24 15:28:12,103] [INFO] DFAST_QC result json was written to GCF_013416295.1_ASM1341629v1_genomic.fna/dqc_result.json
[2024-01-24 15:28:12,104] [INFO] DFAST_QC completed!
[2024-01-24 15:28:12,104] [INFO] Total running time: 0h1m32s