[2024-01-25 18:11:20,526] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:11:20,528] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:11:20,528] [INFO] DQC Reference Directory: /var/lib/cwl/stg8367f04b-157f-4f5f-98b0-9ed88c8d2151/dqc_reference
[2024-01-25 18:11:21,652] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:11:21,653] [INFO] Task started: Prodigal
[2024-01-25 18:11:21,653] [INFO] Running command: gunzip -c /var/lib/cwl/stg39663429-e69c-4989-a5b3-f6ea25717377/GCF_013449735.1_ASM1344973v1_genomic.fna.gz | prodigal -d GCF_013449735.1_ASM1344973v1_genomic.fna/cds.fna -a GCF_013449735.1_ASM1344973v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:11:25,966] [INFO] Task succeeded: Prodigal
[2024-01-25 18:11:25,967] [INFO] Task started: HMMsearch
[2024-01-25 18:11:25,967] [INFO] Running command: hmmsearch --tblout GCF_013449735.1_ASM1344973v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8367f04b-157f-4f5f-98b0-9ed88c8d2151/dqc_reference/reference_markers.hmm GCF_013449735.1_ASM1344973v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:11:26,161] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:11:26,162] [INFO] Found 6/6 markers.
[2024-01-25 18:11:26,187] [INFO] Query marker FASTA was written to GCF_013449735.1_ASM1344973v1_genomic.fna/markers.fasta
[2024-01-25 18:11:26,187] [INFO] Task started: Blastn
[2024-01-25 18:11:26,187] [INFO] Running command: blastn -query GCF_013449735.1_ASM1344973v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg8367f04b-157f-4f5f-98b0-9ed88c8d2151/dqc_reference/reference_markers.fasta -out GCF_013449735.1_ASM1344973v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:11:26,719] [INFO] Task succeeded: Blastn
[2024-01-25 18:11:26,722] [INFO] Selected 11 target genomes.
[2024-01-25 18:11:26,722] [INFO] Target genome list was writen to GCF_013449735.1_ASM1344973v1_genomic.fna/target_genomes.txt
[2024-01-25 18:11:26,736] [INFO] Task started: fastANI
[2024-01-25 18:11:26,736] [INFO] Running command: fastANI --query /var/lib/cwl/stg39663429-e69c-4989-a5b3-f6ea25717377/GCF_013449735.1_ASM1344973v1_genomic.fna.gz --refList GCF_013449735.1_ASM1344973v1_genomic.fna/target_genomes.txt --output GCF_013449735.1_ASM1344973v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:11:32,129] [INFO] Task succeeded: fastANI
[2024-01-25 18:11:32,130] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8367f04b-157f-4f5f-98b0-9ed88c8d2151/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:11:32,130] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8367f04b-157f-4f5f-98b0-9ed88c8d2151/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:11:32,136] [INFO] Found 7 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 18:11:32,136] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:11:32,136] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Lactococcus laudensis	strain=DSM 28961	GCA_013449735.1	1494461	1494461	type	True	100.0	727	729	95	conclusive
Lactococcus laudensis	strain=LMG 28353	GCA_019586025.1	1494461	1494461	type	True	99.9778	701	729	95	conclusive
Lactococcus chungangensis	strain=DSM 22330	GCA_002441885.1	451457	451457	type	True	91.8185	536	729	95	below_threshold
Lactococcus chungangensis	strain=DSM 22330	GCA_900119835.1	451457	451457	type	True	91.7688	546	729	95	below_threshold
Lactococcus raffinolactis	strain=ATCC 43920	GCA_002441745.1	1366	1366	type	True	87.9392	529	729	95	below_threshold
Lactococcus raffinolactis	strain=NBRC 100932	GCA_001591765.1	1366	1366	type	True	87.8557	526	729	95	below_threshold
Lactococcus paracarnosus	strain=TMW 2.1615	GCA_006770285.1	2749962	2749962	type	True	79.6678	258	729	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:11:32,137] [INFO] DFAST Taxonomy check result was written to GCF_013449735.1_ASM1344973v1_genomic.fna/tc_result.tsv
[2024-01-25 18:11:32,138] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:11:32,138] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:11:32,138] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8367f04b-157f-4f5f-98b0-9ed88c8d2151/dqc_reference/checkm_data
[2024-01-25 18:11:32,139] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:11:32,167] [INFO] Task started: CheckM
[2024-01-25 18:11:32,167] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_013449735.1_ASM1344973v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_013449735.1_ASM1344973v1_genomic.fna/checkm_input GCF_013449735.1_ASM1344973v1_genomic.fna/checkm_result
[2024-01-25 18:11:51,371] [INFO] Task succeeded: CheckM
[2024-01-25 18:11:51,373] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:11:51,395] [INFO] ===== Completeness check finished =====
[2024-01-25 18:11:51,395] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:11:51,395] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_013449735.1_ASM1344973v1_genomic.fna/markers.fasta)
[2024-01-25 18:11:51,396] [INFO] Task started: Blastn
[2024-01-25 18:11:51,396] [INFO] Running command: blastn -query GCF_013449735.1_ASM1344973v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg8367f04b-157f-4f5f-98b0-9ed88c8d2151/dqc_reference/reference_markers_gtdb.fasta -out GCF_013449735.1_ASM1344973v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:11:52,192] [INFO] Task succeeded: Blastn
[2024-01-25 18:11:52,195] [INFO] Selected 10 target genomes.
[2024-01-25 18:11:52,195] [INFO] Target genome list was writen to GCF_013449735.1_ASM1344973v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:11:52,222] [INFO] Task started: fastANI
[2024-01-25 18:11:52,222] [INFO] Running command: fastANI --query /var/lib/cwl/stg39663429-e69c-4989-a5b3-f6ea25717377/GCF_013449735.1_ASM1344973v1_genomic.fna.gz --refList GCF_013449735.1_ASM1344973v1_genomic.fna/target_genomes_gtdb.txt --output GCF_013449735.1_ASM1344973v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:11:57,124] [INFO] Task succeeded: fastANI
[2024-01-25 18:11:57,131] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:11:57,131] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_013449735.1	s__Lactococcus_A laudensis	100.0	727	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	97.98	97.98	0.86	0.86	2	conclusive
GCF_002441885.1	s__Lactococcus_A chungangensis	91.8007	537	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	99.24	98.46	0.92	0.88	4	-
GCF_001591765.1	s__Lactococcus_A raffinolactis	87.8859	525	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	99.11	98.57	0.92	0.88	14	-
GCF_002260845.1	s__Lactococcus_A reticulitermitis	84.9381	486	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002441695.1	s__Lactococcus_A piscium	79.9307	254	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_006770285.1	s__Lactococcus_A paracarnosus	79.6793	257	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	99.90	99.90	0.97	0.97	2	-
GCF_006770265.1	s__Lactococcus_A carnosus	79.4749	241	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	97.28	95.96	0.91	0.89	5	-
GCF_011170065.1	s__Lactococcus_A insecticola	78.9947	208	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011170085.1	s__Lactococcus_A hodotermopsidis	78.4212	201	729	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus_A	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 18:11:57,133] [INFO] GTDB search result was written to GCF_013449735.1_ASM1344973v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:11:57,133] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:11:57,135] [INFO] DFAST_QC result json was written to GCF_013449735.1_ASM1344973v1_genomic.fna/dqc_result.json
[2024-01-25 18:11:57,136] [INFO] DFAST_QC completed!
[2024-01-25 18:11:57,136] [INFO] Total running time: 0h0m37s