[2024-01-25 20:20:05,838] [INFO] DFAST_QC pipeline started.
[2024-01-25 20:20:05,839] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 20:20:05,840] [INFO] DQC Reference Directory: /var/lib/cwl/stgbe3e24b3-ccc9-4c25-8d06-eb5cd11c7dc3/dqc_reference
[2024-01-25 20:20:06,940] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 20:20:06,941] [INFO] Task started: Prodigal
[2024-01-25 20:20:06,941] [INFO] Running command: gunzip -c /var/lib/cwl/stga2a7fadf-6245-44d9-9d06-6bceee0a8bf4/GCF_013697085.1_ASM1369708v1_genomic.fna.gz | prodigal -d GCF_013697085.1_ASM1369708v1_genomic.fna/cds.fna -a GCF_013697085.1_ASM1369708v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 20:20:18,483] [INFO] Task succeeded: Prodigal
[2024-01-25 20:20:18,483] [INFO] Task started: HMMsearch
[2024-01-25 20:20:18,484] [INFO] Running command: hmmsearch --tblout GCF_013697085.1_ASM1369708v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgbe3e24b3-ccc9-4c25-8d06-eb5cd11c7dc3/dqc_reference/reference_markers.hmm GCF_013697085.1_ASM1369708v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 20:20:18,793] [INFO] Task succeeded: HMMsearch
[2024-01-25 20:20:18,796] [INFO] Found 6/6 markers.
[2024-01-25 20:20:18,838] [INFO] Query marker FASTA was written to GCF_013697085.1_ASM1369708v1_genomic.fna/markers.fasta
[2024-01-25 20:20:18,838] [INFO] Task started: Blastn
[2024-01-25 20:20:18,839] [INFO] Running command: blastn -query GCF_013697085.1_ASM1369708v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbe3e24b3-ccc9-4c25-8d06-eb5cd11c7dc3/dqc_reference/reference_markers.fasta -out GCF_013697085.1_ASM1369708v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:20:19,832] [INFO] Task succeeded: Blastn
[2024-01-25 20:20:19,835] [INFO] Selected 11 target genomes.
[2024-01-25 20:20:19,836] [INFO] Target genome list was writen to GCF_013697085.1_ASM1369708v1_genomic.fna/target_genomes.txt
[2024-01-25 20:20:19,853] [INFO] Task started: fastANI
[2024-01-25 20:20:19,853] [INFO] Running command: fastANI --query /var/lib/cwl/stga2a7fadf-6245-44d9-9d06-6bceee0a8bf4/GCF_013697085.1_ASM1369708v1_genomic.fna.gz --refList GCF_013697085.1_ASM1369708v1_genomic.fna/target_genomes.txt --output GCF_013697085.1_ASM1369708v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 20:20:31,651] [INFO] Task succeeded: fastANI
[2024-01-25 20:20:31,652] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgbe3e24b3-ccc9-4c25-8d06-eb5cd11c7dc3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 20:20:31,652] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgbe3e24b3-ccc9-4c25-8d06-eb5cd11c7dc3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 20:20:31,659] [INFO] Found 11 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 20:20:31,659] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 20:20:31,660] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Halomonas kenyensis	strain=DSM 17331	GCA_013697085.1	321266	321266	type	True	100.0	1421	1426	95	conclusive
Halomonas kenyensis	strain=DSM 17331	GCA_022341445.1	321266	321266	type	True	99.9993	1426	1426	95	conclusive
Halomonas aerodenitrificans	strain=MCCC 1A11058	GCA_021404405.1	2733483	2733483	type	True	87.0383	1141	1426	95	below_threshold
Halomonas ethanolica	strain=MCCC 1A11081	GCA_021404305.1	2733486	2733486	type	True	86.7815	1117	1426	95	below_threshold
Halomonas lactosivorans	strain=KCTC 52281	GCA_003254665.1	2185141	2185141	type	True	85.4555	1023	1426	95	below_threshold
Halomonas sulfidoxydans	strain=MCCC 1A11059	GCA_017868775.1	2733484	2733484	type	True	85.1856	1046	1426	95	below_threshold
Halomonas tianxiuensis	strain=BC-M4-5	GCA_009834345.1	2497861	2497861	type	True	85.0589	1075	1426	95	below_threshold
Halomonas sulfidivorans	strain=MCCC 1A13718	GCA_017868935.1	2733488	2733488	type	True	84.4025	1000	1426	95	below_threshold
Halomonas antri	strain=Y3S6	GCA_019430905.1	2846777	2846777	type	True	84.2452	999	1426	95	below_threshold
Halomonas bachuensis	strain=DX6	GCA_011742165.1	2717286	2717286	type	True	84.1804	975	1426	95	below_threshold
Halomonas campaniensis	strain=5AG	GCA_014193375.1	213554	213554	type	True	82.7239	807	1426	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 20:20:31,661] [INFO] DFAST Taxonomy check result was written to GCF_013697085.1_ASM1369708v1_genomic.fna/tc_result.tsv
[2024-01-25 20:20:31,661] [INFO] ===== Taxonomy check completed =====
[2024-01-25 20:20:31,661] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 20:20:31,662] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgbe3e24b3-ccc9-4c25-8d06-eb5cd11c7dc3/dqc_reference/checkm_data
[2024-01-25 20:20:31,662] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 20:20:31,705] [INFO] Task started: CheckM
[2024-01-25 20:20:31,705] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_013697085.1_ASM1369708v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_013697085.1_ASM1369708v1_genomic.fna/checkm_input GCF_013697085.1_ASM1369708v1_genomic.fna/checkm_result
[2024-01-25 20:21:08,084] [INFO] Task succeeded: CheckM
[2024-01-25 20:21:08,085] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 20:21:08,134] [INFO] ===== Completeness check finished =====
[2024-01-25 20:21:08,134] [INFO] ===== Start GTDB Search =====
[2024-01-25 20:21:08,134] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_013697085.1_ASM1369708v1_genomic.fna/markers.fasta)
[2024-01-25 20:21:08,134] [INFO] Task started: Blastn
[2024-01-25 20:21:08,134] [INFO] Running command: blastn -query GCF_013697085.1_ASM1369708v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbe3e24b3-ccc9-4c25-8d06-eb5cd11c7dc3/dqc_reference/reference_markers_gtdb.fasta -out GCF_013697085.1_ASM1369708v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:21:09,987] [INFO] Task succeeded: Blastn
[2024-01-25 20:21:09,990] [INFO] Selected 9 target genomes.
[2024-01-25 20:21:09,990] [INFO] Target genome list was writen to GCF_013697085.1_ASM1369708v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 20:21:10,012] [INFO] Task started: fastANI
[2024-01-25 20:21:10,013] [INFO] Running command: fastANI --query /var/lib/cwl/stga2a7fadf-6245-44d9-9d06-6bceee0a8bf4/GCF_013697085.1_ASM1369708v1_genomic.fna.gz --refList GCF_013697085.1_ASM1369708v1_genomic.fna/target_genomes_gtdb.txt --output GCF_013697085.1_ASM1369708v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 20:21:19,808] [INFO] Task succeeded: fastANI
[2024-01-25 20:21:19,815] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 20:21:19,816] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_013697085.1	s__Halomonas kenyensis	100.0	1421	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_011742915.1	s__Halomonas desiderata	87.1581	1123	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	98.30	98.24	0.88	0.88	3	-
GCF_000246875.1	s__Halomonas sp000246875	86.8663	1087	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003254665.1	s__Halomonas lactosivorans	85.449	1024	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_015992245.1	s__Halomonas sp015992245	85.3329	1021	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	99.35	99.35	0.94	0.94	2	-
GCF_009834345.1	s__Halomonas sp009834345	85.0397	1078	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_017868935.1	s__Halomonas sp017868935	84.3917	1000	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011742165.1	s__Halomonas bachuensis	84.168	978	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014193375.1	s__Halomonas campaniensis	82.73	807	1426	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Halomonadaceae;g__Halomonas	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 20:21:19,817] [INFO] GTDB search result was written to GCF_013697085.1_ASM1369708v1_genomic.fna/result_gtdb.tsv
[2024-01-25 20:21:19,817] [INFO] ===== GTDB Search completed =====
[2024-01-25 20:21:19,820] [INFO] DFAST_QC result json was written to GCF_013697085.1_ASM1369708v1_genomic.fna/dqc_result.json
[2024-01-25 20:21:19,820] [INFO] DFAST_QC completed!
[2024-01-25 20:21:19,820] [INFO] Total running time: 0h1m14s