[2024-01-24 15:10:23,019] [INFO] DFAST_QC pipeline started.
[2024-01-24 15:10:23,022] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 15:10:23,022] [INFO] DQC Reference Directory: /var/lib/cwl/stgd1509dd6-d2cf-4e7d-983e-0fc76db76710/dqc_reference
[2024-01-24 15:10:24,567] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 15:10:24,568] [INFO] Task started: Prodigal
[2024-01-24 15:10:24,568] [INFO] Running command: gunzip -c /var/lib/cwl/stg7450189d-f653-4637-9707-6e5770189dae/GCF_014198875.1_ASM1419887v1_genomic.fna.gz | prodigal -d GCF_014198875.1_ASM1419887v1_genomic.fna/cds.fna -a GCF_014198875.1_ASM1419887v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 15:10:47,984] [INFO] Task succeeded: Prodigal
[2024-01-24 15:10:47,985] [INFO] Task started: HMMsearch
[2024-01-24 15:10:47,985] [INFO] Running command: hmmsearch --tblout GCF_014198875.1_ASM1419887v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd1509dd6-d2cf-4e7d-983e-0fc76db76710/dqc_reference/reference_markers.hmm GCF_014198875.1_ASM1419887v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 15:10:48,298] [INFO] Task succeeded: HMMsearch
[2024-01-24 15:10:48,299] [INFO] Found 6/6 markers.
[2024-01-24 15:10:48,347] [INFO] Query marker FASTA was written to GCF_014198875.1_ASM1419887v1_genomic.fna/markers.fasta
[2024-01-24 15:10:48,348] [INFO] Task started: Blastn
[2024-01-24 15:10:48,348] [INFO] Running command: blastn -query GCF_014198875.1_ASM1419887v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd1509dd6-d2cf-4e7d-983e-0fc76db76710/dqc_reference/reference_markers.fasta -out GCF_014198875.1_ASM1419887v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 15:10:49,047] [INFO] Task succeeded: Blastn
[2024-01-24 15:10:49,051] [INFO] Selected 14 target genomes.
[2024-01-24 15:10:49,052] [INFO] Target genome list was writen to GCF_014198875.1_ASM1419887v1_genomic.fna/target_genomes.txt
[2024-01-24 15:10:49,057] [INFO] Task started: fastANI
[2024-01-24 15:10:49,057] [INFO] Running command: fastANI --query /var/lib/cwl/stg7450189d-f653-4637-9707-6e5770189dae/GCF_014198875.1_ASM1419887v1_genomic.fna.gz --refList GCF_014198875.1_ASM1419887v1_genomic.fna/target_genomes.txt --output GCF_014198875.1_ASM1419887v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 15:11:16,128] [INFO] Task succeeded: fastANI
[2024-01-24 15:11:16,129] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd1509dd6-d2cf-4e7d-983e-0fc76db76710/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 15:11:16,129] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd1509dd6-d2cf-4e7d-983e-0fc76db76710/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 15:11:16,140] [INFO] Found 12 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 15:11:16,140] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 15:11:16,141] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Longimicrobium terrae	strain=CECT 8660	GCA_014198875.1	1639882	1639882	type	True	100.0	2233	2235	95	conclusive
Longimicrobium terrae	strain=DSM 29007	GCA_014202995.1	1639882	1639882	type	True	99.9988	2232	2235	95	conclusive
Longimicrobium terrae	strain=CB-286315	GCA_013000925.1	1639882	1639882	type	True	99.9925	2232	2235	95	conclusive
Gemmatimonas groenlandica	strain=TET16	GCA_013004105.1	2732249	2732249	type	True	75.4787	95	2235	95	below_threshold
Gemmatirosa kalamazoonensis	strain=KBS708	GCA_000522985.1	861299	861299	type	True	75.4181	417	2235	95	below_threshold
Corallococcus llansteffanensis	strain=CA051B	GCA_003612055.1	2316731	2316731	type	True	75.3663	350	2235	95	below_threshold
Pyxidicoccus caerfyrddinensis	strain=CA032A	GCA_010894405.1	2709663	2709663	type	True	75.2782	431	2235	95	below_threshold
Myxococcus llanfairpwllgwyngyllgogerychwyrndrobwllllantysiliogogogochensis	strain=AM401	GCA_006636215.1	2590453	2590453	type	True	75.096	305	2235	95	below_threshold
Crenobacter intestini	strain=GY 70310	GCA_004919095.1	2563443	2563443	type	True	74.9987	101	2235	95	below_threshold
Kutzneria kofuensis	strain=DSM 43851	GCA_014203355.1	103725	103725	type	True	74.9057	299	2235	95	below_threshold
Deferrisoma camini	strain=S3R1	GCA_000526155.1	1035120	1035120	type	True	74.893	92	2235	95	below_threshold
Pseudoscardovia radai	strain=DSM 24742	GCA_002259605.1	987066	987066	type	True	74.8058	51	2235	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 15:11:16,142] [INFO] DFAST Taxonomy check result was written to GCF_014198875.1_ASM1419887v1_genomic.fna/tc_result.tsv
[2024-01-24 15:11:16,143] [INFO] ===== Taxonomy check completed =====
[2024-01-24 15:11:16,143] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 15:11:16,143] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd1509dd6-d2cf-4e7d-983e-0fc76db76710/dqc_reference/checkm_data
[2024-01-24 15:11:16,145] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 15:11:16,208] [INFO] Task started: CheckM
[2024-01-24 15:11:16,208] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014198875.1_ASM1419887v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014198875.1_ASM1419887v1_genomic.fna/checkm_input GCF_014198875.1_ASM1419887v1_genomic.fna/checkm_result
[2024-01-24 15:12:30,630] [INFO] Task succeeded: CheckM
[2024-01-24 15:12:30,631] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 15:12:30,651] [INFO] ===== Completeness check finished =====
[2024-01-24 15:12:30,652] [INFO] ===== Start GTDB Search =====
[2024-01-24 15:12:30,652] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014198875.1_ASM1419887v1_genomic.fna/markers.fasta)
[2024-01-24 15:12:30,652] [INFO] Task started: Blastn
[2024-01-24 15:12:30,652] [INFO] Running command: blastn -query GCF_014198875.1_ASM1419887v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd1509dd6-d2cf-4e7d-983e-0fc76db76710/dqc_reference/reference_markers_gtdb.fasta -out GCF_014198875.1_ASM1419887v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 15:12:31,741] [INFO] Task succeeded: Blastn
[2024-01-24 15:12:31,744] [INFO] Selected 18 target genomes.
[2024-01-24 15:12:31,744] [INFO] Target genome list was writen to GCF_014198875.1_ASM1419887v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 15:12:31,760] [INFO] Task started: fastANI
[2024-01-24 15:12:31,761] [INFO] Running command: fastANI --query /var/lib/cwl/stg7450189d-f653-4637-9707-6e5770189dae/GCF_014198875.1_ASM1419887v1_genomic.fna.gz --refList GCF_014198875.1_ASM1419887v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014198875.1_ASM1419887v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 15:12:49,968] [INFO] Task succeeded: fastANI
[2024-01-24 15:12:49,981] [INFO] Found 16 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 15:12:49,981] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014202995.1	s__Longimicrobium terrae	99.9988	2232	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__Longimicrobiaceae;g__Longimicrobium	95.0	100.00	100.00	1.00	1.00	3	conclusive
GCA_902805735.1	s__Longimicrobium sp902805735	78.875	775	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__Longimicrobiaceae;g__Longimicrobium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_019240375.1	s__JAFAYN01 sp019240375	77.7839	355	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__Longimicrobiaceae;g__JAFAYN01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013820915.1	s__JACDHR01 sp013820915	76.5752	207	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__Longimicrobiaceae;g__JACDHR01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_007118565.1	s__SKRI01 sp007118565	76.3485	151	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__Longimicrobiaceae;g__SKRI01	95.0	99.36	99.36	0.87	0.87	2	-
GCA_016741895.1	s__Gemmatimonas sp016741895	75.7804	141	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Gemmatimonadales;f__Gemmatimonadaceae;g__Gemmatimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015492755.1	s__S146-37 sp015492755	75.7096	108	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__UBA6960;g__S146-37	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003242735.1	s__RSA9 sp003242735	75.6386	217	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__RSA9;g__RSA9	95.0	99.82	99.82	0.92	0.92	2	-
GCA_011774835.1	s__KS3-K002 sp011774835	75.6324	90	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__KS3-K002;f__KS3-K002;g__KS3-K002	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016794805.1	s__JADJOM01 sp016794805	75.6216	167	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Gemmatimonadales;f__GWC2-71-9;g__JADJOM01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016713785.1	s__JADJOM01 sp016713785	75.5212	238	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Gemmatimonadales;f__GWC2-71-9;g__JADJOM01	95.0	99.05	98.46	0.95	0.91	10	-
GCA_013002885.1	s__UBA2589 sp013002885	75.4385	66	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__UBA6960;g__UBA2589	95.0	99.00	99.00	0.85	0.85	2	-
GCA_016873995.1	s__UBA2982 sp016873995	75.4343	118	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__UBA6960;g__UBA2982	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017889745.1	s__JAGGAG01 sp017889745	75.3113	106	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Gemmatimonadales;f__GWC2-71-9;g__JAGGAG01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016209885.1	s__JACQVF01 sp016209885	75.2663	114	2235	d__Bacteria;p__Gemmatimonadota;c__Gemmatimonadetes;o__Longimicrobiales;f__UBA6960;g__JACQVF01	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018623495.1	s__JAAZVP01 sp018623495	75.0763	82	2235	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Halothiobacillales;f__JAAZVP01;g__JAAZVP01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 15:12:49,985] [INFO] GTDB search result was written to GCF_014198875.1_ASM1419887v1_genomic.fna/result_gtdb.tsv
[2024-01-24 15:12:49,985] [INFO] ===== GTDB Search completed =====
[2024-01-24 15:12:49,990] [INFO] DFAST_QC result json was written to GCF_014198875.1_ASM1419887v1_genomic.fna/dqc_result.json
[2024-01-24 15:12:49,990] [INFO] DFAST_QC completed!
[2024-01-24 15:12:49,990] [INFO] Total running time: 0h2m27s