[2024-01-24 12:28:35,022] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:28:35,023] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:28:35,023] [INFO] DQC Reference Directory: /var/lib/cwl/stgc853c013-298f-41d6-99e7-aa36ed3e4024/dqc_reference
[2024-01-24 12:28:36,209] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:28:36,210] [INFO] Task started: Prodigal
[2024-01-24 12:28:36,210] [INFO] Running command: gunzip -c /var/lib/cwl/stgc4c1f457-2123-4f08-82fc-fbf9cef4e5ee/GCF_014205215.1_ASM1420521v1_genomic.fna.gz | prodigal -d GCF_014205215.1_ASM1420521v1_genomic.fna/cds.fna -a GCF_014205215.1_ASM1420521v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:29:09,321] [INFO] Task succeeded: Prodigal
[2024-01-24 12:29:09,322] [INFO] Task started: HMMsearch
[2024-01-24 12:29:09,322] [INFO] Running command: hmmsearch --tblout GCF_014205215.1_ASM1420521v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc853c013-298f-41d6-99e7-aa36ed3e4024/dqc_reference/reference_markers.hmm GCF_014205215.1_ASM1420521v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:29:09,732] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:29:09,735] [INFO] Found 6/6 markers.
[2024-01-24 12:29:09,816] [INFO] Query marker FASTA was written to GCF_014205215.1_ASM1420521v1_genomic.fna/markers.fasta
[2024-01-24 12:29:09,817] [INFO] Task started: Blastn
[2024-01-24 12:29:09,817] [INFO] Running command: blastn -query GCF_014205215.1_ASM1420521v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc853c013-298f-41d6-99e7-aa36ed3e4024/dqc_reference/reference_markers.fasta -out GCF_014205215.1_ASM1420521v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:29:10,987] [INFO] Task succeeded: Blastn
[2024-01-24 12:29:10,991] [INFO] Selected 12 target genomes.
[2024-01-24 12:29:10,991] [INFO] Target genome list was writen to GCF_014205215.1_ASM1420521v1_genomic.fna/target_genomes.txt
[2024-01-24 12:29:11,028] [INFO] Task started: fastANI
[2024-01-24 12:29:11,028] [INFO] Running command: fastANI --query /var/lib/cwl/stgc4c1f457-2123-4f08-82fc-fbf9cef4e5ee/GCF_014205215.1_ASM1420521v1_genomic.fna.gz --refList GCF_014205215.1_ASM1420521v1_genomic.fna/target_genomes.txt --output GCF_014205215.1_ASM1420521v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:29:43,785] [INFO] Task succeeded: fastANI
[2024-01-24 12:29:43,786] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc853c013-298f-41d6-99e7-aa36ed3e4024/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:29:43,786] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc853c013-298f-41d6-99e7-aa36ed3e4024/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:29:43,803] [INFO] Found 12 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 12:29:43,803] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:29:43,804] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Actinoplanes lobatus	strain=DSM 43150	GCA_014205215.1	113568	113568	type	True	100.0	3465	3467	95	conclusive
Actinoplanes lobatus	strain=JCM 3061	GCA_014647815.1	113568	113568	type	True	99.9752	3373	3467	95	conclusive
Actinoplanes lobatus	strain=NBRC 12513	GCA_016862255.1	113568	113568	type	True	99.9529	3284	3467	95	conclusive
Actinoplanes capillaceus	strain=NBRC 16408	GCA_016862055.1	76756	76756	type	True	94.9915	2665	3467	95	below_threshold
Actinoplanes campanulatus	strain=NBRC 12511	GCA_016862035.1	113559	113559	type	True	94.9752	2768	3467	95	below_threshold
Actinoplanes campanulatus	strain=JCM 3059	GCA_014647795.1	113559	113559	type	True	94.9677	2776	3467	95	below_threshold
Actinoplanes hulinensis	strain=NEAU-M9	GCA_019429605.1	1144547	1144547	type	True	94.9667	2729	3467	95	below_threshold
Actinoplanes flavus	strain=NEAU-H7	GCA_017592555.1	2820290	2820290	type	True	93.6789	2660	3467	95	below_threshold
Micromonospora acroterricola	strain=5R2A7	GCA_003172955.1	2202421	2202421	type	True	79.142	948	3467	95	below_threshold
Micromonospora phytophila	strain=DSM 105363	GCA_023656545.1	709888	709888	type	True	79.1136	861	3467	95	below_threshold
Dactylosporangium aurantiacum	strain=NRRL B-8018	GCA_025264705.1	35754	35754	type	True	77.5447	1418	3467	95	below_threshold
Dactylosporangium matsuzakiense	strain=NRRL B-16293	GCA_025264725.1	53360	53360	type	True	77.1834	1280	3467	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:29:43,807] [INFO] DFAST Taxonomy check result was written to GCF_014205215.1_ASM1420521v1_genomic.fna/tc_result.tsv
[2024-01-24 12:29:43,807] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:29:43,807] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:29:43,808] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc853c013-298f-41d6-99e7-aa36ed3e4024/dqc_reference/checkm_data
[2024-01-24 12:29:43,808] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:29:43,902] [INFO] Task started: CheckM
[2024-01-24 12:29:43,902] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014205215.1_ASM1420521v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014205215.1_ASM1420521v1_genomic.fna/checkm_input GCF_014205215.1_ASM1420521v1_genomic.fna/checkm_result
[2024-01-24 12:31:55,943] [INFO] Task succeeded: CheckM
[2024-01-24 12:31:55,944] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:31:55,970] [INFO] ===== Completeness check finished =====
[2024-01-24 12:31:55,971] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:31:55,971] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014205215.1_ASM1420521v1_genomic.fna/markers.fasta)
[2024-01-24 12:31:55,971] [INFO] Task started: Blastn
[2024-01-24 12:31:55,972] [INFO] Running command: blastn -query GCF_014205215.1_ASM1420521v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc853c013-298f-41d6-99e7-aa36ed3e4024/dqc_reference/reference_markers_gtdb.fasta -out GCF_014205215.1_ASM1420521v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:31:57,779] [INFO] Task succeeded: Blastn
[2024-01-24 12:31:57,782] [INFO] Selected 10 target genomes.
[2024-01-24 12:31:57,782] [INFO] Target genome list was writen to GCF_014205215.1_ASM1420521v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:31:57,790] [INFO] Task started: fastANI
[2024-01-24 12:31:57,790] [INFO] Running command: fastANI --query /var/lib/cwl/stgc4c1f457-2123-4f08-82fc-fbf9cef4e5ee/GCF_014205215.1_ASM1420521v1_genomic.fna.gz --refList GCF_014205215.1_ASM1420521v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014205215.1_ASM1420521v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:32:27,457] [INFO] Task succeeded: fastANI
[2024-01-24 12:32:27,478] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:32:27,479] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014205215.1	s__Actinoplanes lobatus	100.0	3465	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0428	99.99	99.99	1.00	1.00	3	conclusive
GCF_014647795.1	s__Actinoplanes campanulatus	94.942	2780	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0428	98.93	96.79	0.95	0.87	4	-
GCF_017592555.1	s__Actinoplanes sp017592555	93.6927	2658	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900113015.1	s__Actinoplanes philippinensis	85.0405	2162	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0	100.00	100.00	1.00	1.00	2	-
GCF_016862455.1	s__Actinoplanes utahensis	85.0288	2022	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0	99.99	99.99	0.99	0.99	2	-
GCF_003148685.1	s__Actinoplanes xinjiangensis	84.781	2197	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0	100.00	100.00	1.00	1.00	2	-
GCF_900104725.1	s__Actinoplanes derwentensis	83.9275	2012	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0	100.00	100.00	1.00	1.00	2	-
GCF_015704865.1	s__Actinoplanes sp015704865	83.6461	1985	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000284295.1	s__Actinoplanes missouriensis	83.3946	1865	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018324205.1	s__Actinoplanes ianthinogenes	82.822	1898	3467	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Actinoplanes	95.0	99.99	99.99	0.99	0.99	2	-
--------------------------------------------------------------------------------
[2024-01-24 12:32:27,480] [INFO] GTDB search result was written to GCF_014205215.1_ASM1420521v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:32:27,488] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:32:27,492] [INFO] DFAST_QC result json was written to GCF_014205215.1_ASM1420521v1_genomic.fna/dqc_result.json
[2024-01-24 12:32:27,492] [INFO] DFAST_QC completed!
[2024-01-24 12:32:27,492] [INFO] Total running time: 0h3m52s