[2024-01-25 19:49:05,852] [INFO] DFAST_QC pipeline started.
[2024-01-25 19:49:05,853] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 19:49:05,853] [INFO] DQC Reference Directory: /var/lib/cwl/stg8e7172b2-9de8-4251-aab8-d2dd90247a83/dqc_reference
[2024-01-25 19:49:06,968] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 19:49:06,969] [INFO] Task started: Prodigal
[2024-01-25 19:49:06,969] [INFO] Running command: gunzip -c /var/lib/cwl/stg39872359-c780-408b-92dc-47f681e8579f/GCF_014207695.1_ASM1420769v1_genomic.fna.gz | prodigal -d GCF_014207695.1_ASM1420769v1_genomic.fna/cds.fna -a GCF_014207695.1_ASM1420769v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 19:49:21,720] [INFO] Task succeeded: Prodigal
[2024-01-25 19:49:21,721] [INFO] Task started: HMMsearch
[2024-01-25 19:49:21,721] [INFO] Running command: hmmsearch --tblout GCF_014207695.1_ASM1420769v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg8e7172b2-9de8-4251-aab8-d2dd90247a83/dqc_reference/reference_markers.hmm GCF_014207695.1_ASM1420769v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 19:49:22,006] [INFO] Task succeeded: HMMsearch
[2024-01-25 19:49:22,007] [INFO] Found 6/6 markers.
[2024-01-25 19:49:22,044] [INFO] Query marker FASTA was written to GCF_014207695.1_ASM1420769v1_genomic.fna/markers.fasta
[2024-01-25 19:49:22,045] [INFO] Task started: Blastn
[2024-01-25 19:49:22,045] [INFO] Running command: blastn -query GCF_014207695.1_ASM1420769v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg8e7172b2-9de8-4251-aab8-d2dd90247a83/dqc_reference/reference_markers.fasta -out GCF_014207695.1_ASM1420769v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:49:22,726] [INFO] Task succeeded: Blastn
[2024-01-25 19:49:22,729] [INFO] Selected 18 target genomes.
[2024-01-25 19:49:22,729] [INFO] Target genome list was writen to GCF_014207695.1_ASM1420769v1_genomic.fna/target_genomes.txt
[2024-01-25 19:49:22,749] [INFO] Task started: fastANI
[2024-01-25 19:49:22,750] [INFO] Running command: fastANI --query /var/lib/cwl/stg39872359-c780-408b-92dc-47f681e8579f/GCF_014207695.1_ASM1420769v1_genomic.fna.gz --refList GCF_014207695.1_ASM1420769v1_genomic.fna/target_genomes.txt --output GCF_014207695.1_ASM1420769v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 19:49:37,445] [INFO] Task succeeded: fastANI
[2024-01-25 19:49:37,446] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg8e7172b2-9de8-4251-aab8-d2dd90247a83/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 19:49:37,446] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg8e7172b2-9de8-4251-aab8-d2dd90247a83/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 19:49:37,453] [INFO] Found 11 fastANI hits (3 hits with ANI > threshold)
[2024-01-25 19:49:37,454] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 19:49:37,454] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Pseudoteredinibacter isoporae	strain=DSM 22368	GCA_014207695.1	570281	570281	type	True	100.0	1616	1616	95	conclusive
Pseudoteredinibacter isoporae	strain=LMG 25246	GCA_011602085.1	570281	570281	type	True	99.9997	1615	1616	95	conclusive
Pseudoteredinibacter isoporae	strain=DSM 22368	GCA_011683895.1	570281	570281	type	True	99.9941	1606	1616	95	conclusive
Aestuariicella albida	strain=HHU G3-2	GCA_018863235.1	2842452	2842452	type	True	77.2906	102	1616	95	below_threshold
Aestuariicella hydrocarbonica	strain=JCM 30134	GCA_011602365.1	1470433	1470433	type	True	77.1991	109	1616	95	below_threshold
Agaribacterium haliotis	strain=feces2	GCA_002312815.1	2013869	2013869	type	True	76.9601	60	1616	95	below_threshold
Microbulbifer mangrovi	strain=DD-13	GCA_002009015.1	927787	927787	type	True	76.6399	64	1616	95	below_threshold
Pseudomaricurvus alcaniphilus	strain=MEBiC06469	GCA_011440395.1	1166482	1166482	type	True	76.4872	85	1616	95	below_threshold
Simiduia agarivorans	strain=DSM 21679	GCA_000420285.1	447471	447471	type	True	76.4502	85	1616	95	below_threshold
Microbulbifer celer	strain=KCTC 12973	GCA_020991125.1	435905	435905	type	True	76.4462	58	1616	95	below_threshold
Microbulbifer harenosus	strain=HB161719	GCA_005771435.1	2576840	2576840	type	True	75.9917	59	1616	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 19:49:37,455] [INFO] DFAST Taxonomy check result was written to GCF_014207695.1_ASM1420769v1_genomic.fna/tc_result.tsv
[2024-01-25 19:49:37,455] [INFO] ===== Taxonomy check completed =====
[2024-01-25 19:49:37,456] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 19:49:37,456] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg8e7172b2-9de8-4251-aab8-d2dd90247a83/dqc_reference/checkm_data
[2024-01-25 19:49:37,456] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 19:49:37,502] [INFO] Task started: CheckM
[2024-01-25 19:49:37,502] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014207695.1_ASM1420769v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014207695.1_ASM1420769v1_genomic.fna/checkm_input GCF_014207695.1_ASM1420769v1_genomic.fna/checkm_result
[2024-01-25 19:50:23,908] [INFO] Task succeeded: CheckM
[2024-01-25 19:50:23,909] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 19:50:23,923] [INFO] ===== Completeness check finished =====
[2024-01-25 19:50:23,924] [INFO] ===== Start GTDB Search =====
[2024-01-25 19:50:23,924] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014207695.1_ASM1420769v1_genomic.fna/markers.fasta)
[2024-01-25 19:50:23,925] [INFO] Task started: Blastn
[2024-01-25 19:50:23,925] [INFO] Running command: blastn -query GCF_014207695.1_ASM1420769v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg8e7172b2-9de8-4251-aab8-d2dd90247a83/dqc_reference/reference_markers_gtdb.fasta -out GCF_014207695.1_ASM1420769v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:50:25,144] [INFO] Task succeeded: Blastn
[2024-01-25 19:50:25,146] [INFO] Selected 22 target genomes.
[2024-01-25 19:50:25,147] [INFO] Target genome list was writen to GCF_014207695.1_ASM1420769v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 19:50:25,190] [INFO] Task started: fastANI
[2024-01-25 19:50:25,190] [INFO] Running command: fastANI --query /var/lib/cwl/stg39872359-c780-408b-92dc-47f681e8579f/GCF_014207695.1_ASM1420769v1_genomic.fna.gz --refList GCF_014207695.1_ASM1420769v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014207695.1_ASM1420769v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 19:50:42,645] [INFO] Task succeeded: fastANI
[2024-01-25 19:50:42,654] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 19:50:42,654] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_011602085.1	s__Pseudoteredinibacter isoporae	99.9997	1615	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Pseudoteredinibacter	95.0	100.00	100.00	1.00	0.99	3	conclusive
GCF_018863235.1	s__Pseudomaricurvus sp018863235	77.2906	102	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Pseudomaricurvus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011602365.1	s__Pseudomaricurvus hydrocarbonicus	77.2199	108	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Pseudomaricurvus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011683955.1	s__Pseudomaricurvus alkylphenolicus	77.1147	111	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Pseudomaricurvus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009857815.1	s__Pseudomaricurvus sp009857815	77.0534	96	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Pseudomaricurvus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002312815.1	s__Agaribacterium haliotis	76.9601	60	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Agaribacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002707785.1	s__Pseudomaricurvus sp002707785	76.9366	93	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Pseudomaricurvus	95.0	99.83	99.83	0.94	0.94	2	-
GCF_004363885.1	s__Cellvibrio sp004363885	76.8313	62	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Cellvibrio	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009903625.1	s__Microbulbifer sp009903625	76.758	70	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Microbulbifer	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011440395.1	s__Pseudomaricurvus alcaniphilus	76.5054	84	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Pseudomaricurvus	95.0	98.35	98.35	0.88	0.88	2	-
GCF_001639145.1	s__Microbulbifer sp001639145	76.465	62	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Microbulbifer	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014904735.1	s__Microbulbifer hainanensis	76.195	52	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Microbulbifer	95.0	N/A	N/A	N/A	N/A	1	-
GCF_005771435.1	s__Microbulbifer harenosus	75.9917	59	1616	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Cellvibrionaceae;g__Microbulbifer	95.0	98.17	98.17	0.93	0.93	2	-
--------------------------------------------------------------------------------
[2024-01-25 19:50:42,658] [INFO] GTDB search result was written to GCF_014207695.1_ASM1420769v1_genomic.fna/result_gtdb.tsv
[2024-01-25 19:50:42,659] [INFO] ===== GTDB Search completed =====
[2024-01-25 19:50:42,662] [INFO] DFAST_QC result json was written to GCF_014207695.1_ASM1420769v1_genomic.fna/dqc_result.json
[2024-01-25 19:50:42,662] [INFO] DFAST_QC completed!
[2024-01-25 19:50:42,662] [INFO] Total running time: 0h1m37s