[2024-01-24 12:05:26,608] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:05:26,612] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:05:26,612] [INFO] DQC Reference Directory: /var/lib/cwl/stg7fb3bd9e-9f5e-4b98-bb78-81f6f9a36d46/dqc_reference
[2024-01-24 12:05:30,676] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:05:30,677] [INFO] Task started: Prodigal
[2024-01-24 12:05:30,677] [INFO] Running command: gunzip -c /var/lib/cwl/stgf58d13cd-505c-48f6-b241-a192903ab3a2/GCF_014322765.1_ASM1432276v1_genomic.fna.gz | prodigal -d GCF_014322765.1_ASM1432276v1_genomic.fna/cds.fna -a GCF_014322765.1_ASM1432276v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:05:36,901] [INFO] Task succeeded: Prodigal
[2024-01-24 12:05:36,902] [INFO] Task started: HMMsearch
[2024-01-24 12:05:36,902] [INFO] Running command: hmmsearch --tblout GCF_014322765.1_ASM1432276v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg7fb3bd9e-9f5e-4b98-bb78-81f6f9a36d46/dqc_reference/reference_markers.hmm GCF_014322765.1_ASM1432276v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:05:37,175] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:05:37,177] [INFO] Found 6/6 markers.
[2024-01-24 12:05:37,210] [INFO] Query marker FASTA was written to GCF_014322765.1_ASM1432276v1_genomic.fna/markers.fasta
[2024-01-24 12:05:37,210] [INFO] Task started: Blastn
[2024-01-24 12:05:37,210] [INFO] Running command: blastn -query GCF_014322765.1_ASM1432276v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7fb3bd9e-9f5e-4b98-bb78-81f6f9a36d46/dqc_reference/reference_markers.fasta -out GCF_014322765.1_ASM1432276v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:05:37,935] [INFO] Task succeeded: Blastn
[2024-01-24 12:05:37,939] [INFO] Selected 13 target genomes.
[2024-01-24 12:05:37,940] [INFO] Target genome list was writen to GCF_014322765.1_ASM1432276v1_genomic.fna/target_genomes.txt
[2024-01-24 12:05:37,992] [INFO] Task started: fastANI
[2024-01-24 12:05:37,993] [INFO] Running command: fastANI --query /var/lib/cwl/stgf58d13cd-505c-48f6-b241-a192903ab3a2/GCF_014322765.1_ASM1432276v1_genomic.fna.gz --refList GCF_014322765.1_ASM1432276v1_genomic.fna/target_genomes.txt --output GCF_014322765.1_ASM1432276v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:05:46,833] [INFO] Task succeeded: fastANI
[2024-01-24 12:05:46,833] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg7fb3bd9e-9f5e-4b98-bb78-81f6f9a36d46/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:05:46,833] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg7fb3bd9e-9f5e-4b98-bb78-81f6f9a36d46/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:05:46,844] [INFO] Found 13 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 12:05:46,844] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:05:46,845] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Listeria immobilis	strain=FSL L7-1519	GCA_014322765.1	2713502	2713502	type	True	100.0	1000	1000	95	conclusive
Listeria ivanovii subsp. ivanovii	strain=PAM 55	GCA_000252975.1	202751	1638	claderef	True	86.8399	741	1000	95	below_threshold
Listeria ivanovii subsp. ivanovii	strain=NCTC11846	GCA_900187025.1	202751	1638	type	True	86.8043	754	1000	95	below_threshold
Listeria ivanovii subsp. ivanovii	strain=NCTC11007	GCA_900187195.1	202751	1638	type	True	86.7884	755	1000	95	below_threshold
Listeria ivanovii subsp. ivanovii	strain=WSLC 3010	GCA_000763515.1	202751	1638	type	True	86.7746	758	1000	95	below_threshold
Listeria seeligeri	strain=SLCC3954	GCA_000027145.1	1640	1640	type	True	85.8219	717	1000	95	below_threshold
Listeria innocua	strain=H0996	GCA_017363655.2	1642	1642	claderef	True	83.7916	680	1000	95	below_threshold
Listeria innocua	strain=F4078	GCA_014527425.1	1642	1642	type	True	83.6721	686	1000	95	below_threshold
Listeria innocua	strain=F4078	GCA_024721565.1	1642	1642	type	True	83.6708	678	1000	95	below_threshold
Listeria swaminathanii	strain=FSL L7-0020	GCA_014229645.1	2713501	2713501	type	True	83.6601	669	1000	95	below_threshold
Listeria innocua	strain=2010L-2059	GCA_017363615.2	1642	1642	claderef	True	83.6125	691	1000	95	below_threshold
Listeria monocytogenes	strain=2014L-6393	GCA_013626145.1	1639	1639	claderef	True	83.5991	643	1000	95	below_threshold
Listeria monocytogenes	strain=G4599	GCA_001466295.1	1639	1639	claderef	True	83.517	686	1000	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:05:46,846] [INFO] DFAST Taxonomy check result was written to GCF_014322765.1_ASM1432276v1_genomic.fna/tc_result.tsv
[2024-01-24 12:05:46,847] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:05:46,847] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:05:46,848] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg7fb3bd9e-9f5e-4b98-bb78-81f6f9a36d46/dqc_reference/checkm_data
[2024-01-24 12:05:46,849] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:05:46,883] [INFO] Task started: CheckM
[2024-01-24 12:05:46,883] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014322765.1_ASM1432276v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014322765.1_ASM1432276v1_genomic.fna/checkm_input GCF_014322765.1_ASM1432276v1_genomic.fna/checkm_result
[2024-01-24 12:06:11,554] [INFO] Task succeeded: CheckM
[2024-01-24 12:06:11,556] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:06:11,581] [INFO] ===== Completeness check finished =====
[2024-01-24 12:06:11,581] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:06:11,582] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014322765.1_ASM1432276v1_genomic.fna/markers.fasta)
[2024-01-24 12:06:11,582] [INFO] Task started: Blastn
[2024-01-24 12:06:11,582] [INFO] Running command: blastn -query GCF_014322765.1_ASM1432276v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7fb3bd9e-9f5e-4b98-bb78-81f6f9a36d46/dqc_reference/reference_markers_gtdb.fasta -out GCF_014322765.1_ASM1432276v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:06:12,431] [INFO] Task succeeded: Blastn
[2024-01-24 12:06:12,434] [INFO] Selected 12 target genomes.
[2024-01-24 12:06:12,434] [INFO] Target genome list was writen to GCF_014322765.1_ASM1432276v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:06:12,443] [INFO] Task started: fastANI
[2024-01-24 12:06:12,443] [INFO] Running command: fastANI --query /var/lib/cwl/stgf58d13cd-505c-48f6-b241-a192903ab3a2/GCF_014322765.1_ASM1432276v1_genomic.fna.gz --refList GCF_014322765.1_ASM1432276v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014322765.1_ASM1432276v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:06:20,103] [INFO] Task succeeded: fastANI
[2024-01-24 12:06:20,124] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:06:20,124] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014322765.1	s__Listeria immobilis	100.0	1000	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	99.35	97.95	0.95	0.87	9	conclusive
GCF_000763495.1	s__Listeria londoniensis	88.2272	801	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	99.36	98.73	0.96	0.94	17	-
GCF_900187025.1	s__Listeria ivanovii	86.7878	755	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	99.75	99.44	0.99	0.97	26	-
GCF_000027145.1	s__Listeria seeligeri	85.8219	717	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	97.08	96.23	0.92	0.88	167	-
GCF_014229645.1	s__Listeria sp014229645	83.6601	669	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	N/A	N/A	N/A	N/A	1	-
GCF_015276835.1	s__Listeria innocua	83.6358	681	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	98.16	96.24	0.95	0.92	107	-
GCF_000307025.1	s__Listeria monocytogenes_B	83.5804	686	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	99.46	95.21	0.96	0.90	1699	-
GCF_000060285.1	s__Listeria welshimeri	83.5327	665	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	98.96	98.73	0.93	0.90	49	-
GCF_014229095.1	s__Listeria cayugensis	83.5313	664	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	97.39	97.39	0.93	0.93	2	-
GCF_014224155.1	s__Listeria cossartiae	83.4953	660	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	98.01	97.44	0.96	0.94	9	-
GCF_013282665.1	s__Listeria monocytogenes_C	83.3552	664	1000	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	95.79	95.20	0.93	0.91	176	-
--------------------------------------------------------------------------------
[2024-01-24 12:06:20,126] [INFO] GTDB search result was written to GCF_014322765.1_ASM1432276v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:06:20,127] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:06:20,131] [INFO] DFAST_QC result json was written to GCF_014322765.1_ASM1432276v1_genomic.fna/dqc_result.json
[2024-01-24 12:06:20,131] [INFO] DFAST_QC completed!
[2024-01-24 12:06:20,131] [INFO] Total running time: 0h0m54s