[2024-01-24 13:37:05,345] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:37:05,347] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:37:05,347] [INFO] DQC Reference Directory: /var/lib/cwl/stg85cb9c3d-2a39-47ef-8a38-e4eccd889f8d/dqc_reference
[2024-01-24 13:37:06,685] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:37:06,686] [INFO] Task started: Prodigal
[2024-01-24 13:37:06,686] [INFO] Running command: gunzip -c /var/lib/cwl/stgcac77f3b-56d2-47a9-9b5a-1014448344f8/GCF_014527425.1_ASM1452742v1_genomic.fna.gz | prodigal -d GCF_014527425.1_ASM1452742v1_genomic.fna/cds.fna -a GCF_014527425.1_ASM1452742v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:37:12,618] [INFO] Task succeeded: Prodigal
[2024-01-24 13:37:12,618] [INFO] Task started: HMMsearch
[2024-01-24 13:37:12,618] [INFO] Running command: hmmsearch --tblout GCF_014527425.1_ASM1452742v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg85cb9c3d-2a39-47ef-8a38-e4eccd889f8d/dqc_reference/reference_markers.hmm GCF_014527425.1_ASM1452742v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:37:12,950] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:37:12,951] [INFO] Found 6/6 markers.
[2024-01-24 13:37:12,983] [INFO] Query marker FASTA was written to GCF_014527425.1_ASM1452742v1_genomic.fna/markers.fasta
[2024-01-24 13:37:12,984] [INFO] Task started: Blastn
[2024-01-24 13:37:12,984] [INFO] Running command: blastn -query GCF_014527425.1_ASM1452742v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg85cb9c3d-2a39-47ef-8a38-e4eccd889f8d/dqc_reference/reference_markers.fasta -out GCF_014527425.1_ASM1452742v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:37:13,577] [INFO] Task succeeded: Blastn
[2024-01-24 13:37:13,580] [INFO] Selected 8 target genomes.
[2024-01-24 13:37:13,581] [INFO] Target genome list was writen to GCF_014527425.1_ASM1452742v1_genomic.fna/target_genomes.txt
[2024-01-24 13:37:13,601] [INFO] Task started: fastANI
[2024-01-24 13:37:13,602] [INFO] Running command: fastANI --query /var/lib/cwl/stgcac77f3b-56d2-47a9-9b5a-1014448344f8/GCF_014527425.1_ASM1452742v1_genomic.fna.gz --refList GCF_014527425.1_ASM1452742v1_genomic.fna/target_genomes.txt --output GCF_014527425.1_ASM1452742v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:37:19,167] [INFO] Task succeeded: fastANI
[2024-01-24 13:37:19,168] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg85cb9c3d-2a39-47ef-8a38-e4eccd889f8d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:37:19,168] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg85cb9c3d-2a39-47ef-8a38-e4eccd889f8d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:37:19,177] [INFO] Found 8 fastANI hits (4 hits with ANI > threshold)
[2024-01-24 13:37:19,177] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:37:19,177] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Listeria innocua	strain=F4078	GCA_014527425.1	1642	1642	type	True	100.0	948	948	95	conclusive
Listeria innocua	strain=F4078	GCA_024721565.1	1642	1642	type	True	99.9952	947	948	95	conclusive
Listeria innocua	strain=H0996	GCA_017363655.2	1642	1642	claderef	True	98.8237	887	948	95	conclusive
Listeria innocua	strain=2010L-2059	GCA_017363615.2	1642	1642	claderef	True	96.5377	856	948	95	conclusive
Listeria farberi	strain=FSL L7-0091	GCA_014229245.1	2713500	2713500	type	True	92.4292	831	948	95	below_threshold
Listeria monocytogenes	strain=G4599	GCA_001466295.1	1639	1639	claderef	True	89.3872	829	948	95	below_threshold
Listeria monocytogenes	strain=J0099	GCA_013625995.1	1639	1639	claderef	True	89.3419	833	948	95	below_threshold
Listeria swaminathanii	strain=FSL L7-0020	GCA_014229645.1	2713501	2713501	type	True	88.4731	778	948	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:37:19,178] [INFO] DFAST Taxonomy check result was written to GCF_014527425.1_ASM1452742v1_genomic.fna/tc_result.tsv
[2024-01-24 13:37:19,179] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:37:19,179] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:37:19,179] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg85cb9c3d-2a39-47ef-8a38-e4eccd889f8d/dqc_reference/checkm_data
[2024-01-24 13:37:19,180] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:37:19,217] [INFO] Task started: CheckM
[2024-01-24 13:37:19,217] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014527425.1_ASM1452742v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014527425.1_ASM1452742v1_genomic.fna/checkm_input GCF_014527425.1_ASM1452742v1_genomic.fna/checkm_result
[2024-01-24 13:37:44,247] [INFO] Task succeeded: CheckM
[2024-01-24 13:37:44,248] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:37:44,262] [INFO] ===== Completeness check finished =====
[2024-01-24 13:37:44,263] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:37:44,263] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014527425.1_ASM1452742v1_genomic.fna/markers.fasta)
[2024-01-24 13:37:44,263] [INFO] Task started: Blastn
[2024-01-24 13:37:44,263] [INFO] Running command: blastn -query GCF_014527425.1_ASM1452742v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg85cb9c3d-2a39-47ef-8a38-e4eccd889f8d/dqc_reference/reference_markers_gtdb.fasta -out GCF_014527425.1_ASM1452742v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:37:45,083] [INFO] Task succeeded: Blastn
[2024-01-24 13:37:45,086] [INFO] Selected 10 target genomes.
[2024-01-24 13:37:45,086] [INFO] Target genome list was writen to GCF_014527425.1_ASM1452742v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:37:45,099] [INFO] Task started: fastANI
[2024-01-24 13:37:45,099] [INFO] Running command: fastANI --query /var/lib/cwl/stgcac77f3b-56d2-47a9-9b5a-1014448344f8/GCF_014527425.1_ASM1452742v1_genomic.fna.gz --refList GCF_014527425.1_ASM1452742v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014527425.1_ASM1452742v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:37:52,417] [INFO] Task succeeded: fastANI
[2024-01-24 13:37:52,427] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:37:52,427] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_015276835.1	s__Listeria innocua	98.7901	888	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	98.16	96.24	0.95	0.92	107	conclusive
GCF_014229245.1	s__Listeria farberi	92.4292	831	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	99.35	99.31	0.93	0.92	5	-
GCF_000307025.1	s__Listeria monocytogenes_B	89.325	838	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	99.46	95.21	0.96	0.90	1699	-
GCF_900187225.1	s__Listeria monocytogenes	89.0901	812	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	99.04	95.98	0.97	0.89	1977	-
GCF_013282665.1	s__Listeria monocytogenes_C	88.6845	813	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	95.79	95.20	0.93	0.91	176	-
GCF_014229645.1	s__Listeria sp014229645	88.4731	778	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014224155.1	s__Listeria cossartiae	88.3876	801	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	98.01	97.44	0.96	0.94	9	-
GCF_014229095.1	s__Listeria cayugensis	88.1605	803	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	97.39	97.39	0.93	0.93	2	-
GCF_000060285.1	s__Listeria welshimeri	87.5454	775	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	98.96	98.73	0.93	0.90	49	-
GCF_014322765.1	s__Listeria immobilis	83.6415	678	948	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Listeriaceae;g__Listeria	95.0	99.35	97.95	0.95	0.87	9	-
--------------------------------------------------------------------------------
[2024-01-24 13:37:52,432] [INFO] GTDB search result was written to GCF_014527425.1_ASM1452742v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:37:52,433] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:37:52,436] [INFO] DFAST_QC result json was written to GCF_014527425.1_ASM1452742v1_genomic.fna/dqc_result.json
[2024-01-24 13:37:52,437] [INFO] DFAST_QC completed!
[2024-01-24 13:37:52,437] [INFO] Total running time: 0h0m47s