[2024-01-25 18:15:35,473] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:15:35,475] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:15:35,475] [INFO] DQC Reference Directory: /var/lib/cwl/stg6d849ecd-bc33-4dbd-b8de-5777b5aa7240/dqc_reference
[2024-01-25 18:15:36,629] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:15:36,630] [INFO] Task started: Prodigal
[2024-01-25 18:15:36,630] [INFO] Running command: gunzip -c /var/lib/cwl/stgeb2bf3f4-36da-462a-be03-b59e8e8ab98f/GCF_014534785.1_ASM1453478v1_genomic.fna.gz | prodigal -d GCF_014534785.1_ASM1453478v1_genomic.fna/cds.fna -a GCF_014534785.1_ASM1453478v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:15:45,154] [INFO] Task succeeded: Prodigal
[2024-01-25 18:15:45,155] [INFO] Task started: HMMsearch
[2024-01-25 18:15:45,155] [INFO] Running command: hmmsearch --tblout GCF_014534785.1_ASM1453478v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6d849ecd-bc33-4dbd-b8de-5777b5aa7240/dqc_reference/reference_markers.hmm GCF_014534785.1_ASM1453478v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:15:45,369] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:15:45,371] [INFO] Found 6/6 markers.
[2024-01-25 18:15:45,397] [INFO] Query marker FASTA was written to GCF_014534785.1_ASM1453478v1_genomic.fna/markers.fasta
[2024-01-25 18:15:45,398] [INFO] Task started: Blastn
[2024-01-25 18:15:45,398] [INFO] Running command: blastn -query GCF_014534785.1_ASM1453478v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6d849ecd-bc33-4dbd-b8de-5777b5aa7240/dqc_reference/reference_markers.fasta -out GCF_014534785.1_ASM1453478v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:15:46,271] [INFO] Task succeeded: Blastn
[2024-01-25 18:15:46,273] [INFO] Selected 9 target genomes.
[2024-01-25 18:15:46,274] [INFO] Target genome list was writen to GCF_014534785.1_ASM1453478v1_genomic.fna/target_genomes.txt
[2024-01-25 18:15:46,284] [INFO] Task started: fastANI
[2024-01-25 18:15:46,284] [INFO] Running command: fastANI --query /var/lib/cwl/stgeb2bf3f4-36da-462a-be03-b59e8e8ab98f/GCF_014534785.1_ASM1453478v1_genomic.fna.gz --refList GCF_014534785.1_ASM1453478v1_genomic.fna/target_genomes.txt --output GCF_014534785.1_ASM1453478v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:15:53,571] [INFO] Task succeeded: fastANI
[2024-01-25 18:15:53,571] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6d849ecd-bc33-4dbd-b8de-5777b5aa7240/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:15:53,572] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6d849ecd-bc33-4dbd-b8de-5777b5aa7240/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:15:53,579] [INFO] Found 9 fastANI hits (3 hits with ANI > threshold)
[2024-01-25 18:15:53,579] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:15:53,579] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Aquisalinus flavus	strain=D11M-2	GCA_014534785.1	1526572	1526572	type	True	100.0	1088	1088	95	conclusive
Aquisalinus flavus	strain=D11M-2	GCA_022559625.1	1526572	1526572	type	True	99.9985	1044	1088	95	conclusive
Aquisalinus flavus	strain=CGMCC 1.12921	GCA_014640095.1	1526572	1526572	type	True	99.9981	1088	1088	95	conclusive
Aquisalinus luteolus	strain=NH6-79	GCA_009712425.2	1566827	1566827	type	True	85.9565	895	1088	95	below_threshold
Aquisalinus luteolus	strain=CGMCC 1.14984	GCA_014640455.1	1566827	1566827	type	True	85.9534	902	1088	95	below_threshold
Marinicauda salina	strain=WD6-1	GCA_003122085.1	2135793	2135793	type	True	76.9131	139	1088	95	below_threshold
Rhodovarius crocodyli	strain=CCP-6	GCA_004005855.1	1979269	1979269	type	True	76.2516	101	1088	95	below_threshold
Rhodoligotrophos defluvii	strain=lm1	GCA_005281615.1	2561934	2561934	type	True	76.2386	91	1088	95	below_threshold
Pelagibius marinus	strain=NBU2595	GCA_014925385.1	2762760	2762760	type	True	76.002	115	1088	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:15:53,582] [INFO] DFAST Taxonomy check result was written to GCF_014534785.1_ASM1453478v1_genomic.fna/tc_result.tsv
[2024-01-25 18:15:53,583] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:15:53,583] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:15:53,583] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6d849ecd-bc33-4dbd-b8de-5777b5aa7240/dqc_reference/checkm_data
[2024-01-25 18:15:53,584] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:15:53,619] [INFO] Task started: CheckM
[2024-01-25 18:15:53,619] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014534785.1_ASM1453478v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014534785.1_ASM1453478v1_genomic.fna/checkm_input GCF_014534785.1_ASM1453478v1_genomic.fna/checkm_result
[2024-01-25 18:16:21,573] [INFO] Task succeeded: CheckM
[2024-01-25 18:16:21,574] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:16:21,617] [INFO] ===== Completeness check finished =====
[2024-01-25 18:16:21,617] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:16:21,618] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014534785.1_ASM1453478v1_genomic.fna/markers.fasta)
[2024-01-25 18:16:21,618] [INFO] Task started: Blastn
[2024-01-25 18:16:21,618] [INFO] Running command: blastn -query GCF_014534785.1_ASM1453478v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6d849ecd-bc33-4dbd-b8de-5777b5aa7240/dqc_reference/reference_markers_gtdb.fasta -out GCF_014534785.1_ASM1453478v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:16:23,416] [INFO] Task succeeded: Blastn
[2024-01-25 18:16:23,418] [INFO] Selected 16 target genomes.
[2024-01-25 18:16:23,419] [INFO] Target genome list was writen to GCF_014534785.1_ASM1453478v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:16:23,470] [INFO] Task started: fastANI
[2024-01-25 18:16:23,470] [INFO] Running command: fastANI --query /var/lib/cwl/stgeb2bf3f4-36da-462a-be03-b59e8e8ab98f/GCF_014534785.1_ASM1453478v1_genomic.fna.gz --refList GCF_014534785.1_ASM1453478v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014534785.1_ASM1453478v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:16:34,574] [INFO] Task succeeded: fastANI
[2024-01-25 18:16:34,585] [INFO] Found 16 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:16:34,585] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014640095.1	s__Aquisalinus flavus	99.9981	1088	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Aquisalinus	95.0	100.00	100.00	1.00	1.00	2	conclusive
GCF_009712425.2	s__Aquisalinus luteolus	85.9462	896	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Aquisalinus	95.0	100.00	100.00	1.00	1.00	2	-
GCF_000733125.1	s__Parvularcula oceani	77.4647	259	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Parvularcula	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900199215.1	s__Amphiplicatus metriothermophilus	77.2065	184	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Amphiplicatus	95.0	100.00	100.00	1.00	1.00	2	-
GCF_014199615.1	s__Parvularcula dongshanensis	77.2053	161	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Parvularcula	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002943565.1	s__Marinicaulis flavus	77.1657	210	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Marinicaulis	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013085255.1	s__Parvularcula mediterranea	77.0771	146	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Parvularcula	95.0	N/A	N/A	N/A	N/A	1	-
GCA_001657385.1	s__Marinicaulis sp001657385	77.0459	210	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Marinicaulis	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001426945.1	s__Pseudolabrys sp001426945	76.9274	111	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Xanthobacteraceae;g__Pseudolabrys	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003399445.1	s__Parvularcula sp003399445	76.9199	189	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Parvularcula	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002704915.1	s__Marinicaulis sp002704915	76.6177	174	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Parvularculaceae;g__Marinicaulis	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002869065.1	s__Rhodobium sp002869065	76.482	144	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhodobiaceae;g__Rhodobium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002088275.1	s__Jiella sp002088275	76.3022	156	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Jiella	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002700095.1	s__Jiella sp002700095	76.2441	133	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Jiella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003259195.1	s__Paracoccus sediminilitoris	76.2341	79	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodobacterales;f__Rhodobacteraceae;g__Paracoccus	95.0	95.17	95.17	0.89	0.89	2	-
GCA_018241335.1	s__Palsa-881 sp018241335	76.1862	113	1088	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Palsa-881	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 18:16:34,586] [INFO] GTDB search result was written to GCF_014534785.1_ASM1453478v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:16:34,587] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:16:34,590] [INFO] DFAST_QC result json was written to GCF_014534785.1_ASM1453478v1_genomic.fna/dqc_result.json
[2024-01-25 18:16:34,590] [INFO] DFAST_QC completed!
[2024-01-25 18:16:34,590] [INFO] Total running time: 0h0m59s