[2024-01-24 12:14:49,007] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:14:49,009] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:14:49,009] [INFO] DQC Reference Directory: /var/lib/cwl/stg237f4394-078c-40d4-89c8-21d6015956d1/dqc_reference
[2024-01-24 12:14:50,332] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:14:50,333] [INFO] Task started: Prodigal
[2024-01-24 12:14:50,333] [INFO] Running command: gunzip -c /var/lib/cwl/stg54394207-9137-494f-99c8-539e837e84c6/GCF_014636415.1_ASM1463641v1_genomic.fna.gz | prodigal -d GCF_014636415.1_ASM1463641v1_genomic.fna/cds.fna -a GCF_014636415.1_ASM1463641v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:14:59,066] [INFO] Task succeeded: Prodigal
[2024-01-24 12:14:59,066] [INFO] Task started: HMMsearch
[2024-01-24 12:14:59,066] [INFO] Running command: hmmsearch --tblout GCF_014636415.1_ASM1463641v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg237f4394-078c-40d4-89c8-21d6015956d1/dqc_reference/reference_markers.hmm GCF_014636415.1_ASM1463641v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:14:59,356] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:14:59,357] [INFO] Found 6/6 markers.
[2024-01-24 12:14:59,389] [INFO] Query marker FASTA was written to GCF_014636415.1_ASM1463641v1_genomic.fna/markers.fasta
[2024-01-24 12:14:59,390] [INFO] Task started: Blastn
[2024-01-24 12:14:59,390] [INFO] Running command: blastn -query GCF_014636415.1_ASM1463641v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg237f4394-078c-40d4-89c8-21d6015956d1/dqc_reference/reference_markers.fasta -out GCF_014636415.1_ASM1463641v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:15:00,362] [INFO] Task succeeded: Blastn
[2024-01-24 12:15:00,365] [INFO] Selected 12 target genomes.
[2024-01-24 12:15:00,366] [INFO] Target genome list was writen to GCF_014636415.1_ASM1463641v1_genomic.fna/target_genomes.txt
[2024-01-24 12:15:00,371] [INFO] Task started: fastANI
[2024-01-24 12:15:00,372] [INFO] Running command: fastANI --query /var/lib/cwl/stg54394207-9137-494f-99c8-539e837e84c6/GCF_014636415.1_ASM1463641v1_genomic.fna.gz --refList GCF_014636415.1_ASM1463641v1_genomic.fna/target_genomes.txt --output GCF_014636415.1_ASM1463641v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:15:09,234] [INFO] Task succeeded: fastANI
[2024-01-24 12:15:09,234] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg237f4394-078c-40d4-89c8-21d6015956d1/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:15:09,235] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg237f4394-078c-40d4-89c8-21d6015956d1/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:15:09,246] [INFO] Found 12 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 12:15:09,246] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:15:09,247] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Marinicauda pacifica	strain=CGMCC 1.11031	GCA_014636415.1	1133559	1133559	type	True	100.0	998	998	95	conclusive
Marinicauda pacifica	strain=P-1 km-3	GCA_004793635.1	1133559	1133559	type	True	99.9997	998	998	95	conclusive
Marinicauda pacifica	strain=P-1 km-3	GCA_009806145.1	1133559	1133559	type	True	99.9992	993	998	95	conclusive
Marinicauda algicola	strain=RMAR8-3	GCA_017161425.1	2029849	2029849	type	True	80.377	586	998	95	below_threshold
Marinicauda algicola	strain=JCM 31718	GCA_004793685.1	2029849	2029849	type	True	80.3635	583	998	95	below_threshold
Marinicauda salina	strain=WD6-1	GCA_003122085.1	2135793	2135793	type	True	78.6231	400	998	95	below_threshold
Stappia albiluteola	strain=F7233	GCA_014050225.1	2758565	2758565	type	True	77.0132	96	998	95	below_threshold
Brevundimonas halotolerans	strain=MCS24	GCA_003730275.1	69670	69670	type	True	76.9176	107	998	95	below_threshold
Brevundimonas halotolerans	strain=DSM 24448	GCA_014199165.1	69670	69670	type	True	76.8773	104	998	95	below_threshold
Bradyrhizobium cajani	strain=1010	GCA_009759665.1	1928661	1928661	type	True	76.4526	122	998	95	below_threshold
Sphingomonas changnyeongensis	strain=C33	GCA_009913435.1	2698679	2698679	type	True	76.3593	100	998	95	below_threshold
Bradyrhizobium altum	strain=Pear77	GCA_020889705.1	1571202	1571202	type	True	76.2959	109	998	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:15:09,248] [INFO] DFAST Taxonomy check result was written to GCF_014636415.1_ASM1463641v1_genomic.fna/tc_result.tsv
[2024-01-24 12:15:09,250] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:15:09,250] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:15:09,251] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg237f4394-078c-40d4-89c8-21d6015956d1/dqc_reference/checkm_data
[2024-01-24 12:15:09,252] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:15:09,284] [INFO] Task started: CheckM
[2024-01-24 12:15:09,284] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014636415.1_ASM1463641v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014636415.1_ASM1463641v1_genomic.fna/checkm_input GCF_014636415.1_ASM1463641v1_genomic.fna/checkm_result
[2024-01-24 12:15:39,587] [INFO] Task succeeded: CheckM
[2024-01-24 12:15:39,589] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:15:39,614] [INFO] ===== Completeness check finished =====
[2024-01-24 12:15:39,615] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:15:39,616] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014636415.1_ASM1463641v1_genomic.fna/markers.fasta)
[2024-01-24 12:15:39,616] [INFO] Task started: Blastn
[2024-01-24 12:15:39,616] [INFO] Running command: blastn -query GCF_014636415.1_ASM1463641v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg237f4394-078c-40d4-89c8-21d6015956d1/dqc_reference/reference_markers_gtdb.fasta -out GCF_014636415.1_ASM1463641v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:15:41,463] [INFO] Task succeeded: Blastn
[2024-01-24 12:15:41,468] [INFO] Selected 13 target genomes.
[2024-01-24 12:15:41,468] [INFO] Target genome list was writen to GCF_014636415.1_ASM1463641v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:15:41,481] [INFO] Task started: fastANI
[2024-01-24 12:15:41,482] [INFO] Running command: fastANI --query /var/lib/cwl/stg54394207-9137-494f-99c8-539e837e84c6/GCF_014636415.1_ASM1463641v1_genomic.fna.gz --refList GCF_014636415.1_ASM1463641v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014636415.1_ASM1463641v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:15:49,835] [INFO] Task succeeded: fastANI
[2024-01-24 12:15:49,849] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:15:49,849] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_004793635.1	s__Marinicauda pacifica	99.9997	998	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Marinicauda	95.0	100.00	100.00	1.00	1.00	3	conclusive
GCF_017161425.1	s__Marinicauda algicola	80.3774	586	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Marinicauda	95.0	100.00	100.00	1.00	1.00	2	-
GCF_003122085.1	s__WD6-1 sp003122085	78.6129	401	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__WD6-1	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007050995.1	s__Glycocaulis profundi	78.3571	376	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Glycocaulis	95.0	N/A	N/A	N/A	N/A	1	-
GCF_008630495.1	s__Oceanicaulis satelles	78.2386	329	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Oceanicaulis	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000152745.1	s__Oceanicaulis sp000152745	77.999	348	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Oceanicaulis	95.0	98.46	98.38	0.90	0.88	7	-
GCA_016124675.1	s__Oceanicaulis sp016124675	77.9775	315	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Oceanicaulis	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000420265.1	s__Oceanicaulis alexandrii	77.9202	301	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Oceanicaulis	95.0	97.56	97.05	0.94	0.85	5	-
GCF_000744995.1	s__Glycocaulis sp000744995	77.7763	262	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Glycocaulis	95.0	98.48	98.48	0.95	0.95	2	-
GCF_003046365.1	s__Oceanicaulis sp003046365	77.7056	266	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Oceanicaulis	95.0	96.56	96.56	0.93	0.93	2	-
GCA_001657295.1	s__Oceanicaulis sp001657295	77.4248	287	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Oceanicaulis	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002783385.1	s__Hyphobacterium sp002783385	76.4599	109	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Maricaulaceae;g__Hyphobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016124325.1	s__Phenylobacterium sp016124325	76.1357	146	998	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Caulobacterales;f__Caulobacteraceae;g__Phenylobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:15:49,852] [INFO] GTDB search result was written to GCF_014636415.1_ASM1463641v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:15:49,853] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:15:49,857] [INFO] DFAST_QC result json was written to GCF_014636415.1_ASM1463641v1_genomic.fna/dqc_result.json
[2024-01-24 12:15:49,857] [INFO] DFAST_QC completed!
[2024-01-24 12:15:49,858] [INFO] Total running time: 0h1m1s