[2024-01-25 18:04:50,713] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:04:50,714] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:04:50,714] [INFO] DQC Reference Directory: /var/lib/cwl/stgd20a762e-70f9-455d-8e91-f5f08f7525ba/dqc_reference
[2024-01-25 18:04:51,871] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:04:51,871] [INFO] Task started: Prodigal
[2024-01-25 18:04:51,872] [INFO] Running command: gunzip -c /var/lib/cwl/stg828e477b-e1f1-44e8-a8f7-5456a1651382/GCF_014642775.1_ASM1464277v1_genomic.fna.gz | prodigal -d GCF_014642775.1_ASM1464277v1_genomic.fna/cds.fna -a GCF_014642775.1_ASM1464277v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:05:02,589] [INFO] Task succeeded: Prodigal
[2024-01-25 18:05:02,589] [INFO] Task started: HMMsearch
[2024-01-25 18:05:02,590] [INFO] Running command: hmmsearch --tblout GCF_014642775.1_ASM1464277v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd20a762e-70f9-455d-8e91-f5f08f7525ba/dqc_reference/reference_markers.hmm GCF_014642775.1_ASM1464277v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:05:02,857] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:05:02,858] [INFO] Found 6/6 markers.
[2024-01-25 18:05:02,897] [INFO] Query marker FASTA was written to GCF_014642775.1_ASM1464277v1_genomic.fna/markers.fasta
[2024-01-25 18:05:02,897] [INFO] Task started: Blastn
[2024-01-25 18:05:02,897] [INFO] Running command: blastn -query GCF_014642775.1_ASM1464277v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd20a762e-70f9-455d-8e91-f5f08f7525ba/dqc_reference/reference_markers.fasta -out GCF_014642775.1_ASM1464277v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:05:03,518] [INFO] Task succeeded: Blastn
[2024-01-25 18:05:03,523] [INFO] Selected 12 target genomes.
[2024-01-25 18:05:03,524] [INFO] Target genome list was writen to GCF_014642775.1_ASM1464277v1_genomic.fna/target_genomes.txt
[2024-01-25 18:05:03,528] [INFO] Task started: fastANI
[2024-01-25 18:05:03,528] [INFO] Running command: fastANI --query /var/lib/cwl/stg828e477b-e1f1-44e8-a8f7-5456a1651382/GCF_014642775.1_ASM1464277v1_genomic.fna.gz --refList GCF_014642775.1_ASM1464277v1_genomic.fna/target_genomes.txt --output GCF_014642775.1_ASM1464277v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:05:12,970] [INFO] Task succeeded: fastANI
[2024-01-25 18:05:12,970] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd20a762e-70f9-455d-8e91-f5f08f7525ba/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:05:12,971] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd20a762e-70f9-455d-8e91-f5f08f7525ba/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:05:12,979] [INFO] Found 11 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 18:05:12,979] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:05:12,979] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Gramella forsetii	strain=CGMCC 1.15422	GCA_014642775.1	411153	411153	type	True	100.0	1237	1239	95	conclusive
Gramella forsetii	strain=KT0803	GCA_000060345.1	411153	411153	type	True	99.9986	1238	1239	95	conclusive
Gramella echinicola	strain=DSM 19838	GCA_000423065.1	279359	279359	type	True	80.662	611	1239	95	below_threshold
Gramella gaetbulicola	strain=DSM 23082	GCA_003054225.1	703340	703340	type	True	80.3666	599	1239	95	below_threshold
Gramella sabulilitoris	strain=HSMS-1	GCA_007050985.1	2583991	2583991	type	True	79.8884	489	1239	95	below_threshold
Gramella aestuarii	strain=BS12	GCA_009735585.1	1028746	1028746	type	True	79.8204	474	1239	95	below_threshold
Gramella lutea	strain=YJ019	GCA_022489035.1	1607951	1607951	type	True	79.7543	551	1239	95	below_threshold
Gramella crocea	strain=YB25	GCA_022410465.1	2904124	2904124	type	True	79.0747	454	1239	95	below_threshold
Gramella salexigens	strain=LPB0144	GCA_001889005.1	1913577	1913577	type	True	79.0687	399	1239	95	below_threshold
Salegentibacter lacus	strain=LM13S	GCA_020164555.1	2873599	2873599	type	True	77.5658	273	1239	95	below_threshold
Psychroserpens luteolus	strain=XSD401	GCA_021032705.1	2855840	2855840	type	True	76.4362	61	1239	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:05:12,980] [INFO] DFAST Taxonomy check result was written to GCF_014642775.1_ASM1464277v1_genomic.fna/tc_result.tsv
[2024-01-25 18:05:12,981] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:05:12,981] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:05:12,981] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd20a762e-70f9-455d-8e91-f5f08f7525ba/dqc_reference/checkm_data
[2024-01-25 18:05:12,982] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:05:13,021] [INFO] Task started: CheckM
[2024-01-25 18:05:13,021] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014642775.1_ASM1464277v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014642775.1_ASM1464277v1_genomic.fna/checkm_input GCF_014642775.1_ASM1464277v1_genomic.fna/checkm_result
[2024-01-25 18:05:46,628] [INFO] Task succeeded: CheckM
[2024-01-25 18:05:46,629] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:05:46,644] [INFO] ===== Completeness check finished =====
[2024-01-25 18:05:46,644] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:05:46,644] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014642775.1_ASM1464277v1_genomic.fna/markers.fasta)
[2024-01-25 18:05:46,645] [INFO] Task started: Blastn
[2024-01-25 18:05:46,645] [INFO] Running command: blastn -query GCF_014642775.1_ASM1464277v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd20a762e-70f9-455d-8e91-f5f08f7525ba/dqc_reference/reference_markers_gtdb.fasta -out GCF_014642775.1_ASM1464277v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:05:47,536] [INFO] Task succeeded: Blastn
[2024-01-25 18:05:47,542] [INFO] Selected 7 target genomes.
[2024-01-25 18:05:47,542] [INFO] Target genome list was writen to GCF_014642775.1_ASM1464277v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:05:47,552] [INFO] Task started: fastANI
[2024-01-25 18:05:47,553] [INFO] Running command: fastANI --query /var/lib/cwl/stg828e477b-e1f1-44e8-a8f7-5456a1651382/GCF_014642775.1_ASM1464277v1_genomic.fna.gz --refList GCF_014642775.1_ASM1464277v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014642775.1_ASM1464277v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:05:53,343] [INFO] Task succeeded: fastANI
[2024-01-25 18:05:53,349] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:05:53,349] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000060345.1	s__Gramella forsetii	99.9986	1238	1239	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Gramella	95.0	100.00	100.00	1.00	1.00	2	conclusive
GCF_900105135.1	s__Gramella sp900105135	82.2374	759	1239	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Gramella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000423065.1	s__Gramella echinicola	80.6698	608	1239	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Gramella	95.0	97.44	97.44	0.87	0.87	2	-
GCF_003054225.1	s__Gramella gaetbulicola	80.3571	599	1239	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Gramella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007050985.1	s__Gramella sabulilitoris	79.8999	489	1239	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Gramella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009735585.1	s__Gramella aestuarii	79.7964	478	1239	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Gramella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001889005.1	s__Gramella salexigens	79.0663	399	1239	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Gramella	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 18:05:53,350] [INFO] GTDB search result was written to GCF_014642775.1_ASM1464277v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:05:53,351] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:05:53,358] [INFO] DFAST_QC result json was written to GCF_014642775.1_ASM1464277v1_genomic.fna/dqc_result.json
[2024-01-25 18:05:53,358] [INFO] DFAST_QC completed!
[2024-01-25 18:05:53,359] [INFO] Total running time: 0h1m3s