[2024-01-24 11:43:53,070] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:43:53,072] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:43:53,072] [INFO] DQC Reference Directory: /var/lib/cwl/stg71c6236b-7190-47f9-8830-d777639b8df5/dqc_reference
[2024-01-24 11:43:54,354] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:43:54,355] [INFO] Task started: Prodigal
[2024-01-24 11:43:54,355] [INFO] Running command: gunzip -c /var/lib/cwl/stg094ca163-782c-491e-a690-3a9fafbc0c91/GCF_014645575.1_ASM1464557v1_genomic.fna.gz | prodigal -d GCF_014645575.1_ASM1464557v1_genomic.fna/cds.fna -a GCF_014645575.1_ASM1464557v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:44:03,469] [INFO] Task succeeded: Prodigal
[2024-01-24 11:44:03,470] [INFO] Task started: HMMsearch
[2024-01-24 11:44:03,470] [INFO] Running command: hmmsearch --tblout GCF_014645575.1_ASM1464557v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg71c6236b-7190-47f9-8830-d777639b8df5/dqc_reference/reference_markers.hmm GCF_014645575.1_ASM1464557v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:44:03,667] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:44:03,668] [INFO] Found 6/6 markers.
[2024-01-24 11:44:03,697] [INFO] Query marker FASTA was written to GCF_014645575.1_ASM1464557v1_genomic.fna/markers.fasta
[2024-01-24 11:44:03,697] [INFO] Task started: Blastn
[2024-01-24 11:44:03,698] [INFO] Running command: blastn -query GCF_014645575.1_ASM1464557v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg71c6236b-7190-47f9-8830-d777639b8df5/dqc_reference/reference_markers.fasta -out GCF_014645575.1_ASM1464557v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:44:04,317] [INFO] Task succeeded: Blastn
[2024-01-24 11:44:04,320] [INFO] Selected 6 target genomes.
[2024-01-24 11:44:04,320] [INFO] Target genome list was writen to GCF_014645575.1_ASM1464557v1_genomic.fna/target_genomes.txt
[2024-01-24 11:44:04,323] [INFO] Task started: fastANI
[2024-01-24 11:44:04,323] [INFO] Running command: fastANI --query /var/lib/cwl/stg094ca163-782c-491e-a690-3a9fafbc0c91/GCF_014645575.1_ASM1464557v1_genomic.fna.gz --refList GCF_014645575.1_ASM1464557v1_genomic.fna/target_genomes.txt --output GCF_014645575.1_ASM1464557v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:45:58,932] [INFO] Task succeeded: fastANI
[2024-01-24 11:45:58,932] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg71c6236b-7190-47f9-8830-d777639b8df5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:45:58,932] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg71c6236b-7190-47f9-8830-d777639b8df5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:45:58,939] [INFO] Found 6 fastANI hits (6 hits with ANI > threshold)
[2024-01-24 11:45:58,939] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:45:58,939] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Deinococcus wulumuqiensis	strain=R12	GCA_014875845.1	980427	980427	type	True	99.9853	1077	1097	95	conclusive
Deinococcus wulumuqiensis	strain=R12	GCA_000348665.1	980427	980427	type	True	99.949	977	1097	95	conclusive
Deinococcus wulumuqiensis	strain=R12	GCA_014875835.1	980427	980427	type	True	99.9372	1007	1097	95	conclusive
Deinococcus wulumuqiensis	strain=R12	GCA_014875975.1	980427	980427	type	True	99.9118	1017	1097	95	conclusive
Deinococcus wulumuqiensis	strain=R12	GCA_014875745.1	980427	980427	type	True	99.9099	1035	1097	95	conclusive
Deinococcus wulumuqiensis	strain=R12	GCA_014875875.1	980427	980427	type	True	99.8787	1010	1097	95	conclusive
--------------------------------------------------------------------------------
[2024-01-24 11:45:58,941] [INFO] DFAST Taxonomy check result was written to GCF_014645575.1_ASM1464557v1_genomic.fna/tc_result.tsv
[2024-01-24 11:45:58,941] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:45:58,941] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:45:58,942] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg71c6236b-7190-47f9-8830-d777639b8df5/dqc_reference/checkm_data
[2024-01-24 11:45:58,942] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:45:58,975] [INFO] Task started: CheckM
[2024-01-24 11:45:58,975] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014645575.1_ASM1464557v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014645575.1_ASM1464557v1_genomic.fna/checkm_input GCF_014645575.1_ASM1464557v1_genomic.fna/checkm_result
[2024-01-24 11:46:28,361] [INFO] Task succeeded: CheckM
[2024-01-24 11:46:28,362] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:46:28,389] [INFO] ===== Completeness check finished =====
[2024-01-24 11:46:28,389] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:46:28,390] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014645575.1_ASM1464557v1_genomic.fna/markers.fasta)
[2024-01-24 11:46:28,390] [INFO] Task started: Blastn
[2024-01-24 11:46:28,390] [INFO] Running command: blastn -query GCF_014645575.1_ASM1464557v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg71c6236b-7190-47f9-8830-d777639b8df5/dqc_reference/reference_markers_gtdb.fasta -out GCF_014645575.1_ASM1464557v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:46:29,266] [INFO] Task succeeded: Blastn
[2024-01-24 11:46:29,275] [INFO] Selected 18 target genomes.
[2024-01-24 11:46:29,275] [INFO] Target genome list was writen to GCF_014645575.1_ASM1464557v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:46:29,291] [INFO] Task started: fastANI
[2024-01-24 11:46:29,291] [INFO] Running command: fastANI --query /var/lib/cwl/stg094ca163-782c-491e-a690-3a9fafbc0c91/GCF_014645575.1_ASM1464557v1_genomic.fna.gz --refList GCF_014645575.1_ASM1464557v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014645575.1_ASM1464557v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:46:45,177] [INFO] Task succeeded: fastANI
[2024-01-24 11:46:45,192] [INFO] Found 18 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:46:45,192] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_011067105.1	s__Deinococcus wulumuqiensis	99.9853	1077	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	99.82	97.50	0.97	0.83	19	conclusive
GCF_000008565.1	s__Deinococcus radiodurans	87.3128	821	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	99.94	99.91	0.99	0.99	8	-
GCF_900109185.1	s__Deinococcus reticulitermitis	80.9663	652	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000252445.1	s__Deinococcus gobiensis	80.6075	597	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000599865.1	s__Deinococcus phoenicis	79.8305	536	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002953415.1	s__Deinococcus sp002953415	79.8116	535	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014201885.1	s__Deinococcus budaensis	79.6982	588	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018260275.1	s__Deinococcus sp018260275	79.4387	365	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018260405.1	s__Deinococcus sp018260405	79.3782	428	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004758605.1	s__Deinococcus metallilatus	79.371	536	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	100.00	100.00	0.98	0.98	4	-
GCF_014647075.1	s__Deinococcus aerophilus	79.2172	501	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003444775.1	s__Deinococcus ficus	78.9302	494	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	98.33	97.44	0.95	0.88	4	-
GCF_000190555.1	s__Deinococcus proteolyticus	78.8736	385	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001485435.1	s__Deinococcus grandis	78.8359	519	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	96.65	95.40	0.88	0.83	5	-
GCF_001007995.1	s__Deinococcus soli	78.8113	480	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	98.09	98.03	0.94	0.93	3	-
GCF_000701425.1	s__Deinococcus frigens	78.8059	490	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013337115.1	s__Deinococcus sp013337115	78.7643	505	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014647435.1	s__Deinococcus radiotolerans	78.601	471	1097	d__Bacteria;p__Deinococcota;c__Deinococci;o__Deinococcales;f__Deinococcaceae;g__Deinococcus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:46:45,193] [INFO] GTDB search result was written to GCF_014645575.1_ASM1464557v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:46:45,194] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:46:45,197] [INFO] DFAST_QC result json was written to GCF_014645575.1_ASM1464557v1_genomic.fna/dqc_result.json
[2024-01-24 11:46:45,197] [INFO] DFAST_QC completed!
[2024-01-24 11:46:45,197] [INFO] Total running time: 0h2m52s