[2024-01-24 11:43:42,417] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:43:42,420] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:43:42,421] [INFO] DQC Reference Directory: /var/lib/cwl/stgff276152-f66b-47fa-a91b-a5ff69777f6a/dqc_reference
[2024-01-24 11:43:43,825] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:43:43,826] [INFO] Task started: Prodigal
[2024-01-24 11:43:43,827] [INFO] Running command: gunzip -c /var/lib/cwl/stg35916824-6c45-44d1-976f-1176eb516bae/GCF_014645675.1_ASM1464567v1_genomic.fna.gz | prodigal -d GCF_014645675.1_ASM1464567v1_genomic.fna/cds.fna -a GCF_014645675.1_ASM1464567v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:43:53,334] [INFO] Task succeeded: Prodigal
[2024-01-24 11:43:53,334] [INFO] Task started: HMMsearch
[2024-01-24 11:43:53,334] [INFO] Running command: hmmsearch --tblout GCF_014645675.1_ASM1464567v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgff276152-f66b-47fa-a91b-a5ff69777f6a/dqc_reference/reference_markers.hmm GCF_014645675.1_ASM1464567v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:43:53,657] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:43:53,659] [INFO] Found 6/6 markers.
[2024-01-24 11:43:53,692] [INFO] Query marker FASTA was written to GCF_014645675.1_ASM1464567v1_genomic.fna/markers.fasta
[2024-01-24 11:43:53,693] [INFO] Task started: Blastn
[2024-01-24 11:43:53,693] [INFO] Running command: blastn -query GCF_014645675.1_ASM1464567v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgff276152-f66b-47fa-a91b-a5ff69777f6a/dqc_reference/reference_markers.fasta -out GCF_014645675.1_ASM1464567v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:43:54,838] [INFO] Task succeeded: Blastn
[2024-01-24 11:43:54,841] [INFO] Selected 11 target genomes.
[2024-01-24 11:43:54,841] [INFO] Target genome list was writen to GCF_014645675.1_ASM1464567v1_genomic.fna/target_genomes.txt
[2024-01-24 11:43:54,846] [INFO] Task started: fastANI
[2024-01-24 11:43:54,847] [INFO] Running command: fastANI --query /var/lib/cwl/stg35916824-6c45-44d1-976f-1176eb516bae/GCF_014645675.1_ASM1464567v1_genomic.fna.gz --refList GCF_014645675.1_ASM1464567v1_genomic.fna/target_genomes.txt --output GCF_014645675.1_ASM1464567v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:44:04,536] [INFO] Task succeeded: fastANI
[2024-01-24 11:44:04,537] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgff276152-f66b-47fa-a91b-a5ff69777f6a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:44:04,537] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgff276152-f66b-47fa-a91b-a5ff69777f6a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:44:04,547] [INFO] Found 11 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 11:44:04,548] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:44:04,548] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Luteimonas terricola	strain=CGMCC 1.8985	GCA_014645675.1	645597	645597	type	True	100.0	1007	1007	95	conclusive
Luteimonas terricola	strain=BZ92r	GCA_004352845.1	645597	645597	type	True	99.9791	1000	1007	95	conclusive
Luteimonas arsenica	strain=26-35	GCA_004352825.1	1586242	1586242	type	True	88.0523	762	1007	95	below_threshold
Luteimonas padinae	strain=KCTC 52403	GCA_014652935.1	1714359	1714359	type	True	87.4137	786	1007	95	below_threshold
Luteimonas colneyensis	strain=Sa2BVA3	GCA_014836665.1	2762230	2762230	type	True	87.3344	772	1007	95	below_threshold
Luteimonas granuli	strain=Gr-4	GCA_007795095.1	1176533	1176533	type	True	86.448	741	1007	95	below_threshold
Luteimonas lumbrici	strain=1.1416	GCA_006476065.1	2559601	2559601	type	True	82.4548	521	1007	95	below_threshold
Luteimonas aquatica	strain=RIB1-20	GCA_022662575.1	450364	450364	type	True	82.3434	603	1007	95	below_threshold
Luteimonas weifangensis	strain=WF-2	GCA_003416885.1	2303539	2303539	type	True	82.2206	553	1007	95	below_threshold
Luteimonas saliphila	strain=SJ-9	GCA_016774335.1	2804919	2804919	type	True	82.2159	648	1007	95	below_threshold
Luteimonas salinisoli	strain=SJ-92	GCA_013425525.1	2752307	2752307	type	True	81.8389	657	1007	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:44:04,549] [INFO] DFAST Taxonomy check result was written to GCF_014645675.1_ASM1464567v1_genomic.fna/tc_result.tsv
[2024-01-24 11:44:04,550] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:44:04,550] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:44:04,550] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgff276152-f66b-47fa-a91b-a5ff69777f6a/dqc_reference/checkm_data
[2024-01-24 11:44:04,552] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:44:04,587] [INFO] Task started: CheckM
[2024-01-24 11:44:04,588] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014645675.1_ASM1464567v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014645675.1_ASM1464567v1_genomic.fna/checkm_input GCF_014645675.1_ASM1464567v1_genomic.fna/checkm_result
[2024-01-24 11:44:44,799] [INFO] Task succeeded: CheckM
[2024-01-24 11:44:44,800] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 97.92%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:44:44,820] [INFO] ===== Completeness check finished =====
[2024-01-24 11:44:44,820] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:44:44,821] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014645675.1_ASM1464567v1_genomic.fna/markers.fasta)
[2024-01-24 11:44:44,821] [INFO] Task started: Blastn
[2024-01-24 11:44:44,821] [INFO] Running command: blastn -query GCF_014645675.1_ASM1464567v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgff276152-f66b-47fa-a91b-a5ff69777f6a/dqc_reference/reference_markers_gtdb.fasta -out GCF_014645675.1_ASM1464567v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:44:46,906] [INFO] Task succeeded: Blastn
[2024-01-24 11:44:46,909] [INFO] Selected 8 target genomes.
[2024-01-24 11:44:46,909] [INFO] Target genome list was writen to GCF_014645675.1_ASM1464567v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:44:46,917] [INFO] Task started: fastANI
[2024-01-24 11:44:46,917] [INFO] Running command: fastANI --query /var/lib/cwl/stg35916824-6c45-44d1-976f-1176eb516bae/GCF_014645675.1_ASM1464567v1_genomic.fna.gz --refList GCF_014645675.1_ASM1464567v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014645675.1_ASM1464567v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:44:53,960] [INFO] Task succeeded: fastANI
[2024-01-24 11:44:53,973] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:44:53,973] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_004352845.1	s__Luteimonas terricola	99.9791	1000	1007	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Luteimonas	95.0	99.99	99.99	0.99	0.99	2	conclusive
GCF_004352825.1	s__Luteimonas arsenica	88.0707	763	1007	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Luteimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014652935.1	s__Luteimonas padinae	87.4231	785	1007	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Luteimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014836665.1	s__Luteimonas sp014836665	87.3344	772	1007	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Luteimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016615955.1	s__Luteimonas sp016615955	86.9896	754	1007	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Luteimonas	95.0	99.39	98.78	0.97	0.95	3	-
GCF_007795095.1	s__Luteimonas granuli	86.4693	739	1007	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Luteimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014764385.1	s__Luteimonas sp014212025	84.9391	719	1007	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Luteimonas	95.0	99.36	98.72	0.98	0.96	3	-
GCF_016615815.1	s__Luteimonas sp016615815	84.9157	723	1007	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Luteimonas	95.0	99.14	98.29	0.98	0.95	3	-
--------------------------------------------------------------------------------
[2024-01-24 11:44:53,975] [INFO] GTDB search result was written to GCF_014645675.1_ASM1464567v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:44:53,976] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:44:53,978] [INFO] DFAST_QC result json was written to GCF_014645675.1_ASM1464567v1_genomic.fna/dqc_result.json
[2024-01-24 11:44:53,979] [INFO] DFAST_QC completed!
[2024-01-24 11:44:53,979] [INFO] Total running time: 0h1m12s