[2024-01-24 13:58:41,505] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:58:41,507] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:58:41,507] [INFO] DQC Reference Directory: /var/lib/cwl/stgfc808270-65d0-4cd8-8606-2604d45cc809/dqc_reference
[2024-01-24 13:58:42,896] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:58:42,897] [INFO] Task started: Prodigal
[2024-01-24 13:58:42,897] [INFO] Running command: gunzip -c /var/lib/cwl/stg2d79ea0b-e76f-4fcd-9605-3e61fcdb36cc/GCF_014647035.1_ASM1464703v1_genomic.fna.gz | prodigal -d GCF_014647035.1_ASM1464703v1_genomic.fna/cds.fna -a GCF_014647035.1_ASM1464703v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:58:51,468] [INFO] Task succeeded: Prodigal
[2024-01-24 13:58:51,469] [INFO] Task started: HMMsearch
[2024-01-24 13:58:51,469] [INFO] Running command: hmmsearch --tblout GCF_014647035.1_ASM1464703v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfc808270-65d0-4cd8-8606-2604d45cc809/dqc_reference/reference_markers.hmm GCF_014647035.1_ASM1464703v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:58:51,790] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:58:51,792] [INFO] Found 6/6 markers.
[2024-01-24 13:58:51,824] [INFO] Query marker FASTA was written to GCF_014647035.1_ASM1464703v1_genomic.fna/markers.fasta
[2024-01-24 13:58:51,824] [INFO] Task started: Blastn
[2024-01-24 13:58:51,824] [INFO] Running command: blastn -query GCF_014647035.1_ASM1464703v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgfc808270-65d0-4cd8-8606-2604d45cc809/dqc_reference/reference_markers.fasta -out GCF_014647035.1_ASM1464703v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:58:52,435] [INFO] Task succeeded: Blastn
[2024-01-24 13:58:52,439] [INFO] Selected 22 target genomes.
[2024-01-24 13:58:52,440] [INFO] Target genome list was writen to GCF_014647035.1_ASM1464703v1_genomic.fna/target_genomes.txt
[2024-01-24 13:58:52,466] [INFO] Task started: fastANI
[2024-01-24 13:58:52,466] [INFO] Running command: fastANI --query /var/lib/cwl/stg2d79ea0b-e76f-4fcd-9605-3e61fcdb36cc/GCF_014647035.1_ASM1464703v1_genomic.fna.gz --refList GCF_014647035.1_ASM1464703v1_genomic.fna/target_genomes.txt --output GCF_014647035.1_ASM1464703v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:59:08,499] [INFO] Task succeeded: fastANI
[2024-01-24 13:59:08,500] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgfc808270-65d0-4cd8-8606-2604d45cc809/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:59:08,500] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgfc808270-65d0-4cd8-8606-2604d45cc809/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:59:08,512] [INFO] Found 5 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 13:59:08,512] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:59:08,512] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Sporolactobacillus putidus	strain=JCM 15325	GCA_014647035.1	492735	492735	type	True	100.0	1139	1141	95	conclusive
Sporolactobacillus nakayamae	strain=ATCC 700379	GCA_900113325.1	269670	269670	type	True	80.2917	126	1141	95	below_threshold
Sporolactobacillus inulinus	strain=NBRC 13595	GCA_006539285.1	2078	2078	type	True	80.2576	130	1141	95	below_threshold
Sporolactobacillus pectinivorans	strain=GD201205	GCA_002802965.1	1591408	1591408	type	True	79.477	260	1141	95	below_threshold
Sporolactobacillus terrae	strain=DSM 11697	GCA_000648615.1	269673	269673	type	True	77.6937	102	1141	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:59:08,514] [INFO] DFAST Taxonomy check result was written to GCF_014647035.1_ASM1464703v1_genomic.fna/tc_result.tsv
[2024-01-24 13:59:08,514] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:59:08,514] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:59:08,514] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfc808270-65d0-4cd8-8606-2604d45cc809/dqc_reference/checkm_data
[2024-01-24 13:59:08,515] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:59:08,559] [INFO] Task started: CheckM
[2024-01-24 13:59:08,559] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014647035.1_ASM1464703v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014647035.1_ASM1464703v1_genomic.fna/checkm_input GCF_014647035.1_ASM1464703v1_genomic.fna/checkm_result
[2024-01-24 13:59:40,221] [INFO] Task succeeded: CheckM
[2024-01-24 13:59:40,223] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:59:40,244] [INFO] ===== Completeness check finished =====
[2024-01-24 13:59:40,244] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:59:40,245] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014647035.1_ASM1464703v1_genomic.fna/markers.fasta)
[2024-01-24 13:59:40,246] [INFO] Task started: Blastn
[2024-01-24 13:59:40,246] [INFO] Running command: blastn -query GCF_014647035.1_ASM1464703v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgfc808270-65d0-4cd8-8606-2604d45cc809/dqc_reference/reference_markers_gtdb.fasta -out GCF_014647035.1_ASM1464703v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:59:41,016] [INFO] Task succeeded: Blastn
[2024-01-24 13:59:41,021] [INFO] Selected 20 target genomes.
[2024-01-24 13:59:41,021] [INFO] Target genome list was writen to GCF_014647035.1_ASM1464703v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:59:41,092] [INFO] Task started: fastANI
[2024-01-24 13:59:41,092] [INFO] Running command: fastANI --query /var/lib/cwl/stg2d79ea0b-e76f-4fcd-9605-3e61fcdb36cc/GCF_014647035.1_ASM1464703v1_genomic.fna.gz --refList GCF_014647035.1_ASM1464703v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014647035.1_ASM1464703v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:59:52,410] [INFO] Task succeeded: fastANI
[2024-01-24 13:59:52,424] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:59:52,424] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014647035.1	s__Sporolactobacillus putidus	100.0	1139	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_006539285.1	s__Sporolactobacillus inulinus	80.3023	129	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	99.52	99.06	0.91	0.89	3	-
GCF_900113325.1	s__Sporolactobacillus nakayamae	80.2917	126	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002802965.1	s__Sporolactobacillus pectinivorans	79.477	260	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004153165.1	s__Sporolactobacillus sp004153165	79.254	230	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000497245.1	s__Sporolactobacillus laevolacticus	77.7425	174	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000648615.1	s__Sporolactobacillus terrae	77.6937	102	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	98.89	98.59	0.91	0.87	6	-
GCF_004153225.1	s__Sporolactobacillus sp004153225	77.2372	178	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000377985.1	s__Sporolactobacillus vineae	77.2003	152	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	99.97	99.97	0.99	0.99	2	-
GCF_004153195.1	s__Sporolactobacillus sp004153195	77.1808	143	1141	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_G;f__Sporolactobacillaceae;g__Sporolactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:59:52,426] [INFO] GTDB search result was written to GCF_014647035.1_ASM1464703v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:59:52,427] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:59:52,429] [INFO] DFAST_QC result json was written to GCF_014647035.1_ASM1464703v1_genomic.fna/dqc_result.json
[2024-01-24 13:59:52,430] [INFO] DFAST_QC completed!
[2024-01-24 13:59:52,430] [INFO] Total running time: 0h1m11s