[2024-01-24 14:30:31,207] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:30:31,209] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:30:31,209] [INFO] DQC Reference Directory: /var/lib/cwl/stg28201f6e-34f5-4040-8b3c-7930b3af8ef5/dqc_reference
[2024-01-24 14:30:33,801] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:30:33,802] [INFO] Task started: Prodigal
[2024-01-24 14:30:33,802] [INFO] Running command: gunzip -c /var/lib/cwl/stgd78a23ad-0eb5-4128-8e82-0363605fde9e/GCF_014647375.1_ASM1464737v1_genomic.fna.gz | prodigal -d GCF_014647375.1_ASM1464737v1_genomic.fna/cds.fna -a GCF_014647375.1_ASM1464737v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:31:06,212] [INFO] Task succeeded: Prodigal
[2024-01-24 14:31:06,213] [INFO] Task started: HMMsearch
[2024-01-24 14:31:06,213] [INFO] Running command: hmmsearch --tblout GCF_014647375.1_ASM1464737v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg28201f6e-34f5-4040-8b3c-7930b3af8ef5/dqc_reference/reference_markers.hmm GCF_014647375.1_ASM1464737v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:31:06,662] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:31:06,664] [INFO] Found 6/6 markers.
[2024-01-24 14:31:06,720] [INFO] Query marker FASTA was written to GCF_014647375.1_ASM1464737v1_genomic.fna/markers.fasta
[2024-01-24 14:31:06,720] [INFO] Task started: Blastn
[2024-01-24 14:31:06,720] [INFO] Running command: blastn -query GCF_014647375.1_ASM1464737v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg28201f6e-34f5-4040-8b3c-7930b3af8ef5/dqc_reference/reference_markers.fasta -out GCF_014647375.1_ASM1464737v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:31:07,406] [INFO] Task succeeded: Blastn
[2024-01-24 14:31:07,409] [INFO] Selected 11 target genomes.
[2024-01-24 14:31:07,410] [INFO] Target genome list was writen to GCF_014647375.1_ASM1464737v1_genomic.fna/target_genomes.txt
[2024-01-24 14:31:07,422] [INFO] Task started: fastANI
[2024-01-24 14:31:07,423] [INFO] Running command: fastANI --query /var/lib/cwl/stgd78a23ad-0eb5-4128-8e82-0363605fde9e/GCF_014647375.1_ASM1464737v1_genomic.fna.gz --refList GCF_014647375.1_ASM1464737v1_genomic.fna/target_genomes.txt --output GCF_014647375.1_ASM1464737v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:31:22,075] [INFO] Task succeeded: fastANI
[2024-01-24 14:31:22,076] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg28201f6e-34f5-4040-8b3c-7930b3af8ef5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:31:22,076] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg28201f6e-34f5-4040-8b3c-7930b3af8ef5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:31:22,090] [INFO] Found 11 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 14:31:22,090] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:31:22,090] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Parabacteroides faecis	strain=JCM 18682	GCA_014647375.1	1217282	1217282	type	True	100.0	2205	2206	95	conclusive
Parabacteroides faecis	strain=DSM 102983	GCA_014199665.1	1217282	1217282	type	True	99.9975	2200	2206	95	conclusive
Parabacteroides timonensis	strain=Marseille-P3236	GCA_900128505.1	1871013	1871013	type	True	85.0445	1197	2206	95	below_threshold
Parabacteroides goldsteinii	strain=DSM 19448	GCA_000969835.1	328812	328812	type	True	84.4285	1407	2206	95	below_threshold
Parabacteroides gordonii	strain=DSM 23371	GCA_000428565.1	574930	574930	type	True	82.4625	1103	2206	95	below_threshold
Parabacteroides gordonii	strain=MS-1	GCA_000969825.1	574930	574930	type	True	82.4219	1121	2206	95	below_threshold
Parabacteroides gordonii	strain=FDAARGOS_1522	GCA_020297465.1	574930	574930	type	True	82.3873	1132	2206	95	below_threshold
Parabacteroides johnsonii	strain=DSM 18315	GCA_025151045.1	387661	387661	type	True	79.6781	519	2206	95	below_threshold
Parabacteroides merdae	strain=ATCC 43184	GCA_025151215.1	46503	46503	type	True	79.5814	525	2206	95	below_threshold
Parabacteroides acidifaciens	strain=426_9	GCA_014385205.1	2290935	2290935	type	True	79.4824	569	2206	95	below_threshold
Phocaeicola intestinalis	strain=Sa1CVN1	GCA_014837065.1	2762212	2762212	type	True	79.0872	63	2206	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:31:22,092] [INFO] DFAST Taxonomy check result was written to GCF_014647375.1_ASM1464737v1_genomic.fna/tc_result.tsv
[2024-01-24 14:31:22,093] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:31:22,093] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:31:22,093] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg28201f6e-34f5-4040-8b3c-7930b3af8ef5/dqc_reference/checkm_data
[2024-01-24 14:31:22,095] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:31:22,160] [INFO] Task started: CheckM
[2024-01-24 14:31:22,160] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014647375.1_ASM1464737v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014647375.1_ASM1464737v1_genomic.fna/checkm_input GCF_014647375.1_ASM1464737v1_genomic.fna/checkm_result
[2024-01-24 14:32:48,066] [INFO] Task succeeded: CheckM
[2024-01-24 14:32:48,067] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:32:48,092] [INFO] ===== Completeness check finished =====
[2024-01-24 14:32:48,092] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:32:48,093] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014647375.1_ASM1464737v1_genomic.fna/markers.fasta)
[2024-01-24 14:32:48,093] [INFO] Task started: Blastn
[2024-01-24 14:32:48,094] [INFO] Running command: blastn -query GCF_014647375.1_ASM1464737v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg28201f6e-34f5-4040-8b3c-7930b3af8ef5/dqc_reference/reference_markers_gtdb.fasta -out GCF_014647375.1_ASM1464737v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:32:48,996] [INFO] Task succeeded: Blastn
[2024-01-24 14:32:49,000] [INFO] Selected 10 target genomes.
[2024-01-24 14:32:49,000] [INFO] Target genome list was writen to GCF_014647375.1_ASM1464737v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:32:49,009] [INFO] Task started: fastANI
[2024-01-24 14:32:49,009] [INFO] Running command: fastANI --query /var/lib/cwl/stgd78a23ad-0eb5-4128-8e82-0363605fde9e/GCF_014647375.1_ASM1464737v1_genomic.fna.gz --refList GCF_014647375.1_ASM1464737v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014647375.1_ASM1464737v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:33:01,524] [INFO] Task succeeded: fastANI
[2024-01-24 14:33:01,535] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:33:01,535] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014647375.1	s__Parabacteroides faecis	100.0	2205	2206	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	98.52	97.91	0.91	0.84	9	conclusive
GCF_003480915.1	s__Parabacteroides sp003480915	88.6808	1336	2206	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	99.39	97.67	0.93	0.87	5	-
GCF_900128505.1	s__Parabacteroides timonensis	85.0356	1198	2206	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	97.14	96.11	0.85	0.79	3	-
GCF_015550595.1	s__Parabacteroides sp900540715	84.635	1221	2206	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	99.96	99.96	0.98	0.97	3	-
GCF_000969835.1	s__Parabacteroides goldsteinii	84.4411	1405	2206	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	98.03	95.33	0.85	0.78	37	-
GCA_905196875.1	s__Parabacteroides sp905196875	83.1058	1055	2206	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000969825.1	s__Parabacteroides gordonii	82.4025	1124	2206	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	98.64	96.69	0.92	0.81	6	-
GCF_000156495.1	s__Parabacteroides johnsonii	79.3484	525	2206	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Bacteroidales;f__Tannerellaceae;g__Parabacteroides	95.0	98.42	96.67	0.88	0.77	13	-
--------------------------------------------------------------------------------
[2024-01-24 14:33:01,537] [INFO] GTDB search result was written to GCF_014647375.1_ASM1464737v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:33:01,537] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:33:01,540] [INFO] DFAST_QC result json was written to GCF_014647375.1_ASM1464737v1_genomic.fna/dqc_result.json
[2024-01-24 14:33:01,541] [INFO] DFAST_QC completed!
[2024-01-24 14:33:01,541] [INFO] Total running time: 0h2m30s