[2024-01-25 17:35:20,321] [INFO] DFAST_QC pipeline started.
[2024-01-25 17:35:20,323] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 17:35:20,323] [INFO] DQC Reference Directory: /var/lib/cwl/stgbe879e5c-74c7-4c5e-a649-66770373d036/dqc_reference
[2024-01-25 17:35:21,517] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 17:35:21,518] [INFO] Task started: Prodigal
[2024-01-25 17:35:21,518] [INFO] Running command: gunzip -c /var/lib/cwl/stg84d13b58-5b07-4854-b3d6-47e4e46bb532/GCF_014647475.1_ASM1464747v1_genomic.fna.gz | prodigal -d GCF_014647475.1_ASM1464747v1_genomic.fna/cds.fna -a GCF_014647475.1_ASM1464747v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 17:35:34,456] [INFO] Task succeeded: Prodigal
[2024-01-25 17:35:34,457] [INFO] Task started: HMMsearch
[2024-01-25 17:35:34,457] [INFO] Running command: hmmsearch --tblout GCF_014647475.1_ASM1464747v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgbe879e5c-74c7-4c5e-a649-66770373d036/dqc_reference/reference_markers.hmm GCF_014647475.1_ASM1464747v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 17:35:34,656] [INFO] Task succeeded: HMMsearch
[2024-01-25 17:35:34,657] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg84d13b58-5b07-4854-b3d6-47e4e46bb532/GCF_014647475.1_ASM1464747v1_genomic.fna.gz]
[2024-01-25 17:35:34,690] [INFO] Query marker FASTA was written to GCF_014647475.1_ASM1464747v1_genomic.fna/markers.fasta
[2024-01-25 17:35:34,691] [INFO] Task started: Blastn
[2024-01-25 17:35:34,691] [INFO] Running command: blastn -query GCF_014647475.1_ASM1464747v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbe879e5c-74c7-4c5e-a649-66770373d036/dqc_reference/reference_markers.fasta -out GCF_014647475.1_ASM1464747v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:35:35,214] [INFO] Task succeeded: Blastn
[2024-01-25 17:35:35,217] [INFO] Selected 7 target genomes.
[2024-01-25 17:35:35,218] [INFO] Target genome list was writen to GCF_014647475.1_ASM1464747v1_genomic.fna/target_genomes.txt
[2024-01-25 17:35:35,222] [INFO] Task started: fastANI
[2024-01-25 17:35:35,222] [INFO] Running command: fastANI --query /var/lib/cwl/stg84d13b58-5b07-4854-b3d6-47e4e46bb532/GCF_014647475.1_ASM1464747v1_genomic.fna.gz --refList GCF_014647475.1_ASM1464747v1_genomic.fna/target_genomes.txt --output GCF_014647475.1_ASM1464747v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 17:35:40,828] [INFO] Task succeeded: fastANI
[2024-01-25 17:35:40,829] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgbe879e5c-74c7-4c5e-a649-66770373d036/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 17:35:40,829] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgbe879e5c-74c7-4c5e-a649-66770373d036/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 17:35:40,835] [INFO] Found 7 fastANI hits (0 hits with ANI > threshold)
[2024-01-25 17:35:40,835] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-25 17:35:40,835] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Halarchaeum grantii	strain=JCM 19585	GCA_014647455.2	1193105	1193105	type	True	80.5109	550	1116	95	below_threshold
Halarchaeum rubridurum	strain=JCM 16108	GCA_014647115.1	489911	489911	type	True	79.9563	536	1116	95	below_threshold
Halarchaeum rubridurum	strain=DSM 22443	GCA_017873775.1	489911	489911	type	True	79.9404	533	1116	95	below_threshold
Halobacterium hubeiense	strain=JI20-1	GCA_001488575.1	1407499	1407499	type	True	79.581	494	1116	95	below_threshold
Halarchaeum acidiphilum	strain=JCM 16109	GCA_000474235.1	489138	489138	type	True	79.3418	466	1116	95	below_threshold
Halobacterium litoreum	strain=ZS-54-S2	GCA_021233415.1	2039234	2039234	type	True	78.9635	497	1116	95	below_threshold
Natronomonas salina	strain=YPL13	GCA_013391105.1	1710540	1710540	type	True	77.3551	395	1116	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 17:35:40,837] [INFO] DFAST Taxonomy check result was written to GCF_014647475.1_ASM1464747v1_genomic.fna/tc_result.tsv
[2024-01-25 17:35:40,837] [INFO] ===== Taxonomy check completed =====
[2024-01-25 17:35:40,837] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 17:35:40,838] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgbe879e5c-74c7-4c5e-a649-66770373d036/dqc_reference/checkm_data
[2024-01-25 17:35:40,838] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 17:35:40,874] [INFO] Task started: CheckM
[2024-01-25 17:35:40,875] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014647475.1_ASM1464747v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014647475.1_ASM1464747v1_genomic.fna/checkm_input GCF_014647475.1_ASM1464747v1_genomic.fna/checkm_result
[2024-01-25 17:36:17,086] [INFO] Task succeeded: CheckM
[2024-01-25 17:36:17,087] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 17:36:17,106] [INFO] ===== Completeness check finished =====
[2024-01-25 17:36:17,107] [INFO] ===== Start GTDB Search =====
[2024-01-25 17:36:17,108] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014647475.1_ASM1464747v1_genomic.fna/markers.fasta)
[2024-01-25 17:36:17,108] [INFO] Task started: Blastn
[2024-01-25 17:36:17,108] [INFO] Running command: blastn -query GCF_014647475.1_ASM1464747v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbe879e5c-74c7-4c5e-a649-66770373d036/dqc_reference/reference_markers_gtdb.fasta -out GCF_014647475.1_ASM1464747v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:36:17,651] [INFO] Task succeeded: Blastn
[2024-01-25 17:36:17,654] [INFO] Selected 7 target genomes.
[2024-01-25 17:36:17,654] [INFO] Target genome list was writen to GCF_014647475.1_ASM1464747v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 17:36:17,659] [INFO] Task started: fastANI
[2024-01-25 17:36:17,659] [INFO] Running command: fastANI --query /var/lib/cwl/stg84d13b58-5b07-4854-b3d6-47e4e46bb532/GCF_014647475.1_ASM1464747v1_genomic.fna.gz --refList GCF_014647475.1_ASM1464747v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014647475.1_ASM1464747v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 17:36:23,779] [INFO] Task succeeded: fastANI
[2024-01-25 17:36:23,785] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 17:36:23,785] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014647475.1	s__Halocalculus aciditolerans	100.0	1116	1116	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Halobacteriaceae;g__Halocalculus	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_014647455.2	s__Halarchaeum grantii	80.6338	541	1116	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Halobacteriaceae;g__Halarchaeum	95.0	95.76	95.76	0.83	0.83	2	-
GCF_014647115.1	s__Halarchaeum rubridurum	79.9976	532	1116	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Halobacteriaceae;g__Halarchaeum	95.0	100.00	100.00	1.00	1.00	2	-
GCF_014647155.1	s__Halarchaeum nitratireducens	79.7539	529	1116	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Halobacteriaceae;g__Halarchaeum	95.0	98.88	98.88	0.86	0.86	2	-
GCF_017874335.1	s__Halarchaeum salinum	79.6683	441	1116	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Halobacteriaceae;g__Halarchaeum	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001488575.1	s__Halobacterium hubeiense	79.6142	489	1116	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Halobacteriaceae;g__Halobacterium	95.0	99.52	99.49	0.94	0.94	8	-
GCF_011734195.1	s__Halobacterium sp011734195	79.1207	513	1116	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Halobacteriaceae;g__Halobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 17:36:23,786] [INFO] GTDB search result was written to GCF_014647475.1_ASM1464747v1_genomic.fna/result_gtdb.tsv
[2024-01-25 17:36:23,787] [INFO] ===== GTDB Search completed =====
[2024-01-25 17:36:23,789] [INFO] DFAST_QC result json was written to GCF_014647475.1_ASM1464747v1_genomic.fna/dqc_result.json
[2024-01-25 17:36:23,789] [INFO] DFAST_QC completed!
[2024-01-25 17:36:23,789] [INFO] Total running time: 0h1m3s