[2024-01-24 11:19:55,113] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:19:55,114] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:19:55,115] [INFO] DQC Reference Directory: /var/lib/cwl/stg51db1baf-e3bd-4526-9e37-7a14045f069c/dqc_reference
[2024-01-24 11:19:56,462] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:19:56,463] [INFO] Task started: Prodigal
[2024-01-24 11:19:56,463] [INFO] Running command: gunzip -c /var/lib/cwl/stg7c3b5023-531c-48ba-9f51-2bcd90764c62/GCF_014648615.1_ASM1464861v1_genomic.fna.gz | prodigal -d GCF_014648615.1_ASM1464861v1_genomic.fna/cds.fna -a GCF_014648615.1_ASM1464861v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:20:22,123] [INFO] Task succeeded: Prodigal
[2024-01-24 11:20:22,124] [INFO] Task started: HMMsearch
[2024-01-24 11:20:22,124] [INFO] Running command: hmmsearch --tblout GCF_014648615.1_ASM1464861v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg51db1baf-e3bd-4526-9e37-7a14045f069c/dqc_reference/reference_markers.hmm GCF_014648615.1_ASM1464861v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:20:22,491] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:20:22,492] [INFO] Found 6/6 markers.
[2024-01-24 11:20:22,565] [INFO] Query marker FASTA was written to GCF_014648615.1_ASM1464861v1_genomic.fna/markers.fasta
[2024-01-24 11:20:22,565] [INFO] Task started: Blastn
[2024-01-24 11:20:22,565] [INFO] Running command: blastn -query GCF_014648615.1_ASM1464861v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg51db1baf-e3bd-4526-9e37-7a14045f069c/dqc_reference/reference_markers.fasta -out GCF_014648615.1_ASM1464861v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:20:23,911] [INFO] Task succeeded: Blastn
[2024-01-24 11:20:23,915] [INFO] Selected 11 target genomes.
[2024-01-24 11:20:23,916] [INFO] Target genome list was writen to GCF_014648615.1_ASM1464861v1_genomic.fna/target_genomes.txt
[2024-01-24 11:20:23,922] [INFO] Task started: fastANI
[2024-01-24 11:20:23,922] [INFO] Running command: fastANI --query /var/lib/cwl/stg7c3b5023-531c-48ba-9f51-2bcd90764c62/GCF_014648615.1_ASM1464861v1_genomic.fna.gz --refList GCF_014648615.1_ASM1464861v1_genomic.fna/target_genomes.txt --output GCF_014648615.1_ASM1464861v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:20:49,065] [INFO] Task succeeded: fastANI
[2024-01-24 11:20:49,066] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg51db1baf-e3bd-4526-9e37-7a14045f069c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:20:49,067] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg51db1baf-e3bd-4526-9e37-7a14045f069c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:20:49,076] [INFO] Found 11 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 11:20:49,077] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:20:49,077] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Streptomyces nojiriensis	strain=JCM 3382	GCA_014648615.1	66374	66374	type	True	100.0	2940	2941	95	conclusive
Streptomyces nojiriensis	strain=JCM 3382	GCA_017639205.1	66374	66374	type	True	99.9985	2935	2941	95	conclusive
Streptomyces nojiriensis	strain=NBRC 13794	GCA_016755855.1	66374	66374	type	True	99.9982	2938	2941	95	conclusive
Streptomyces spororaveus	strain=NBRC 15456	GCA_016755875.1	284039	284039	type	True	93.3983	2311	2941	95	below_threshold
Streptomyces avidinii	strain=DSM 40526	GCA_017874515.1	1895	1895	type	True	92.2094	2209	2941	95	below_threshold
Streptomyces virginiae	strain=NRRL ISP-5094	GCA_000720455.1	1961	1961	suspected-type	True	89.6841	2089	2941	95	below_threshold
Streptomyces formicae	strain=1H-GS9	GCA_022647665.1	1616117	1616117	type	True	81.917	1353	2941	95	below_threshold
Streptomyces somaliensis	strain=DSM 40738	GCA_024349285.1	78355	78355	type	True	81.4711	1038	2941	95	below_threshold
Streptomyces lichenis	strain=LCR6-01	GCA_023218175.1	2306967	2306967	type	True	81.3304	1330	2941	95	below_threshold
Streptomyces sennicomposti	strain=RCPT1-4	GCA_019890635.1	2873384	2873384	type	True	81.1836	1277	2941	95	below_threshold
Streptomyces harenosi	strain=PRKS01-65	GCA_011008945.1	2697029	2697029	type	True	81.1311	1160	2941	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:20:49,078] [INFO] DFAST Taxonomy check result was written to GCF_014648615.1_ASM1464861v1_genomic.fna/tc_result.tsv
[2024-01-24 11:20:49,079] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:20:49,079] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:20:49,079] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg51db1baf-e3bd-4526-9e37-7a14045f069c/dqc_reference/checkm_data
[2024-01-24 11:20:49,080] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:20:49,164] [INFO] Task started: CheckM
[2024-01-24 11:20:49,164] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014648615.1_ASM1464861v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014648615.1_ASM1464861v1_genomic.fna/checkm_input GCF_014648615.1_ASM1464861v1_genomic.fna/checkm_result
[2024-01-24 11:22:42,862] [INFO] Task succeeded: CheckM
[2024-01-24 11:22:42,863] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 5.21%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:22:42,890] [INFO] ===== Completeness check finished =====
[2024-01-24 11:22:42,891] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:22:42,891] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014648615.1_ASM1464861v1_genomic.fna/markers.fasta)
[2024-01-24 11:22:42,892] [INFO] Task started: Blastn
[2024-01-24 11:22:42,892] [INFO] Running command: blastn -query GCF_014648615.1_ASM1464861v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg51db1baf-e3bd-4526-9e37-7a14045f069c/dqc_reference/reference_markers_gtdb.fasta -out GCF_014648615.1_ASM1464861v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:22:45,013] [INFO] Task succeeded: Blastn
[2024-01-24 11:22:45,017] [INFO] Selected 10 target genomes.
[2024-01-24 11:22:45,017] [INFO] Target genome list was writen to GCF_014648615.1_ASM1464861v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:22:45,027] [INFO] Task started: fastANI
[2024-01-24 11:22:45,027] [INFO] Running command: fastANI --query /var/lib/cwl/stg7c3b5023-531c-48ba-9f51-2bcd90764c62/GCF_014648615.1_ASM1464861v1_genomic.fna.gz --refList GCF_014648615.1_ASM1464861v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014648615.1_ASM1464861v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:23:09,733] [INFO] Task succeeded: fastANI
[2024-01-24 11:23:09,742] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:23:09,743] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_017639205.1	s__Streptomyces nojiriensis	99.9985	2935	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	100.00	100.00	1.00	1.00	3	conclusive
GCF_008642275.1	s__Streptomyces venezuelae_E	93.5135	2214	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016755875.1	s__Streptomyces spororaveus	93.4006	2311	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000716685.1	s__Streptomyces virginiae_A	93.3562	2333	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	97.46	95.91	0.89	0.84	3	-
GCF_003148825.1	s__Streptomyces sp003148825	93.0004	2309	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	95.84	95.84	0.87	0.86	3	-
GCF_002080455.1	s__Streptomyces sp002080455	92.2403	2225	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	98.34	98.21	0.92	0.90	7	-
GCF_000725555.1	s__Streptomyces erythrochromogenes	90.0163	1973	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	95.80	95.80	0.85	0.85	2	-
GCF_014648795.1	s__Streptomyces cinnamonensis	89.5481	2089	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	98.18	96.61	0.93	0.87	24	-
GCF_001005295.1	s__Streptomyces yangpuensis	89.2729	2006	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	96.05	95.28	0.86	0.84	3	-
GCF_000612545.1	s__Streptomyces sp000612545	88.6276	1919	2941	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:23:09,744] [INFO] GTDB search result was written to GCF_014648615.1_ASM1464861v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:23:09,745] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:23:09,748] [INFO] DFAST_QC result json was written to GCF_014648615.1_ASM1464861v1_genomic.fna/dqc_result.json
[2024-01-24 11:23:09,749] [INFO] DFAST_QC completed!
[2024-01-24 11:23:09,749] [INFO] Total running time: 0h3m15s