[2024-01-25 17:44:35,626] [INFO] DFAST_QC pipeline started.
[2024-01-25 17:44:35,643] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 17:44:35,643] [INFO] DQC Reference Directory: /var/lib/cwl/stgcdd716e7-d756-462c-a029-1bf6eca4fb72/dqc_reference
[2024-01-25 17:44:36,819] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 17:44:36,820] [INFO] Task started: Prodigal
[2024-01-25 17:44:36,820] [INFO] Running command: gunzip -c /var/lib/cwl/stgc68c0d65-ec3e-42ba-bb13-cb4aef5401fc/GCF_014649155.1_ASM1464915v1_genomic.fna.gz | prodigal -d GCF_014649155.1_ASM1464915v1_genomic.fna/cds.fna -a GCF_014649155.1_ASM1464915v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 17:44:58,645] [INFO] Task succeeded: Prodigal
[2024-01-25 17:44:58,645] [INFO] Task started: HMMsearch
[2024-01-25 17:44:58,645] [INFO] Running command: hmmsearch --tblout GCF_014649155.1_ASM1464915v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgcdd716e7-d756-462c-a029-1bf6eca4fb72/dqc_reference/reference_markers.hmm GCF_014649155.1_ASM1464915v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 17:44:58,920] [INFO] Task succeeded: HMMsearch
[2024-01-25 17:44:58,922] [INFO] Found 6/6 markers.
[2024-01-25 17:44:58,980] [INFO] Query marker FASTA was written to GCF_014649155.1_ASM1464915v1_genomic.fna/markers.fasta
[2024-01-25 17:44:58,980] [INFO] Task started: Blastn
[2024-01-25 17:44:58,980] [INFO] Running command: blastn -query GCF_014649155.1_ASM1464915v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgcdd716e7-d756-462c-a029-1bf6eca4fb72/dqc_reference/reference_markers.fasta -out GCF_014649155.1_ASM1464915v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:45:00,198] [INFO] Task succeeded: Blastn
[2024-01-25 17:45:00,202] [INFO] Selected 18 target genomes.
[2024-01-25 17:45:00,203] [INFO] Target genome list was writen to GCF_014649155.1_ASM1464915v1_genomic.fna/target_genomes.txt
[2024-01-25 17:45:00,231] [INFO] Task started: fastANI
[2024-01-25 17:45:00,231] [INFO] Running command: fastANI --query /var/lib/cwl/stgc68c0d65-ec3e-42ba-bb13-cb4aef5401fc/GCF_014649155.1_ASM1464915v1_genomic.fna.gz --refList GCF_014649155.1_ASM1464915v1_genomic.fna/target_genomes.txt --output GCF_014649155.1_ASM1464915v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 17:45:39,126] [INFO] Task succeeded: fastANI
[2024-01-25 17:45:39,126] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgcdd716e7-d756-462c-a029-1bf6eca4fb72/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 17:45:39,127] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgcdd716e7-d756-462c-a029-1bf6eca4fb72/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 17:45:39,137] [INFO] Found 18 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 17:45:39,137] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 17:45:39,137] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Streptomyces daghestanicus	strain=JCM 4365	GCA_014649475.1	66885	66885	suspected-type	True	99.5627	2463	2565	95	conclusive
Streptomyces daghestanicus	strain=NBRC 12762	GCA_020521275.1	66885	66885	suspected-type	True	99.5519	2483	2565	95	conclusive
Streptomyces fumanus	strain=JCM 4477	GCA_014655295.1	67302	67302	type	True	88.0035	1808	2565	95	below_threshold
Streptomyces griseicoloratus	strain=TRM S81-3	GCA_014534645.1	2752516	2752516	type	True	86.5533	1707	2565	95	below_threshold
Streptomyces flaveolus	strain=JCM 4032	GCA_014648815.1	67297	67297	type	True	86.551	1725	2565	95	below_threshold
Streptomyces solaniscabiei	strain=FS70	GCA_020312315.1	2683255	2683255	type	True	86.4867	1702	2565	95	below_threshold
Streptomyces rubrogriseus	strain=NBRC 15455	GCA_003112595.1	194673	194673	type	True	86.1919	1758	2565	95	below_threshold
Streptomyces harenosi	strain=PRKS01-65	GCA_011008945.1	2697029	2697029	type	True	85.982	1496	2565	95	below_threshold
Streptomyces griseostramineus	strain=CECT 3273	GCA_014203535.1	66898	66898	suspected-type	True	85.517	1655	2565	95	below_threshold
Streptomyces calvus	strain=JCM 4326	GCA_014649315.1	67282	67282	type	True	85.4375	1668	2565	95	below_threshold
Streptomyces calvus	strain=CECT 3271	GCA_014138735.1	67282	67282	type	True	85.4322	1677	2565	95	below_threshold
Streptomyces albaduncus	strain=JCM 4715	GCA_014650615.1	68172	68172	type	True	85.2641	1659	2565	95	below_threshold
Streptomyces albaduncus	strain=CECT 3226	GCA_014203495.1	68172	68172	type	True	85.212	1686	2565	95	below_threshold
Streptomyces lomondensis	strain=DSM 41428	GCA_021440105.1	68229	68229	type	True	85.1931	1677	2565	95	below_threshold
Streptomyces indiaensis	strain=DSM 43803	GCA_021474405.1	284033	284033	type	True	85.081	1478	2565	95	below_threshold
Streptomyces griseoflavus	strain=JCM 4479	GCA_014649995.1	35619	35619	type	True	85.0724	1577	2565	95	below_threshold
Streptomyces griseosporeus	strain=JCM 4766	GCA_014656055.1	1910	1910	type	True	84.9714	1675	2565	95	below_threshold
Streptomyces sennicomposti	strain=RCPT1-4	GCA_019890635.1	2873384	2873384	type	True	84.832	1467	2565	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 17:45:39,139] [INFO] DFAST Taxonomy check result was written to GCF_014649155.1_ASM1464915v1_genomic.fna/tc_result.tsv
[2024-01-25 17:45:39,139] [INFO] ===== Taxonomy check completed =====
[2024-01-25 17:45:39,139] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 17:45:39,139] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgcdd716e7-d756-462c-a029-1bf6eca4fb72/dqc_reference/checkm_data
[2024-01-25 17:45:39,140] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 17:45:39,215] [INFO] Task started: CheckM
[2024-01-25 17:45:39,215] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014649155.1_ASM1464915v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014649155.1_ASM1464915v1_genomic.fna/checkm_input GCF_014649155.1_ASM1464915v1_genomic.fna/checkm_result
[2024-01-25 17:47:39,320] [INFO] Task succeeded: CheckM
[2024-01-25 17:47:39,321] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 1.04%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 17:47:39,343] [INFO] ===== Completeness check finished =====
[2024-01-25 17:47:39,344] [INFO] ===== Start GTDB Search =====
[2024-01-25 17:47:39,345] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014649155.1_ASM1464915v1_genomic.fna/markers.fasta)
[2024-01-25 17:47:39,345] [INFO] Task started: Blastn
[2024-01-25 17:47:39,345] [INFO] Running command: blastn -query GCF_014649155.1_ASM1464915v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgcdd716e7-d756-462c-a029-1bf6eca4fb72/dqc_reference/reference_markers_gtdb.fasta -out GCF_014649155.1_ASM1464915v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:47:41,581] [INFO] Task succeeded: Blastn
[2024-01-25 17:47:41,584] [INFO] Selected 18 target genomes.
[2024-01-25 17:47:41,584] [INFO] Target genome list was writen to GCF_014649155.1_ASM1464915v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 17:47:41,622] [INFO] Task started: fastANI
[2024-01-25 17:47:41,623] [INFO] Running command: fastANI --query /var/lib/cwl/stgc68c0d65-ec3e-42ba-bb13-cb4aef5401fc/GCF_014649155.1_ASM1464915v1_genomic.fna.gz --refList GCF_014649155.1_ASM1464915v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014649155.1_ASM1464915v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 17:48:20,201] [INFO] Task succeeded: fastANI
[2024-01-25 17:48:20,212] [INFO] Found 18 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 17:48:20,212] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_014649155.1	s__Streptomyces griseoviridis	100.0	2561	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	99.52	99.48	0.96	0.96	3	conclusive
GCF_018614415.1	s__Streptomyces sp018614415	90.083	1843	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014655295.1	s__Streptomyces fumanus	88.0436	1803	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001905385.1	s__Streptomyces sp001905385	86.6421	1679	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	96.01	96.01	0.83	0.83	2	-
GCA_002155895.1	s__Streptomyces rochei	86.5889	1509	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	98.80	98.25	0.91	0.79	28	-
GCF_014648815.1	s__Streptomyces flaveolus	86.5753	1722	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002005565.1	s__Streptomyces sp002005565	86.5636	1624	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCA_000720845.1	s__Streptomyces sp000720845	86.3982	1710	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003248315.1	s__Streptomyces sp003248315	86.3843	1666	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000717995.1	s__Streptomyces violaceorubidus	86.326	1686	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003112555.1	s__Streptomyces coelicoflavus	86.2045	1710	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	97.84	97.03	0.90	0.86	6	-
GCF_000721235.1	s__Streptomyces olivaceus	85.9434	1702	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	98.53	98.10	0.91	0.89	6	-
GCF_000721605.1	s__Streptomyces sp000721605	85.5636	1642	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009852415.1	s__Streptomyces sp009852415	85.5108	1726	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001905725.1	s__Streptomyces sp001905725	85.3989	1690	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018069565.1	s__Streptomyces sp018069565	85.3886	1531	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	N/A	N/A	N/A	N/A	1	-
GCA_014650015.1	s__Streptomyces griseoloalbus	85.1444	1678	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	99.17	99.17	0.94	0.94	3	-
GCF_014656055.1	s__Streptomyces griseosporeus	84.9657	1676	2565	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Streptomycetales;f__Streptomycetaceae;g__Streptomyces	95.0	98.70	98.69	0.95	0.95	3	-
--------------------------------------------------------------------------------
[2024-01-25 17:48:20,214] [INFO] GTDB search result was written to GCF_014649155.1_ASM1464915v1_genomic.fna/result_gtdb.tsv
[2024-01-25 17:48:20,215] [INFO] ===== GTDB Search completed =====
[2024-01-25 17:48:20,218] [INFO] DFAST_QC result json was written to GCF_014649155.1_ASM1464915v1_genomic.fna/dqc_result.json
[2024-01-25 17:48:20,219] [INFO] DFAST_QC completed!
[2024-01-25 17:48:20,219] [INFO] Total running time: 0h3m45s