[2024-01-25 17:45:20,690] [INFO] DFAST_QC pipeline started.
[2024-01-25 17:45:20,691] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 17:45:20,691] [INFO] DQC Reference Directory: /var/lib/cwl/stg2fb21fbf-7044-4ff7-b9c2-73aee3e5b824/dqc_reference
[2024-01-25 17:45:21,833] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 17:45:21,834] [INFO] Task started: Prodigal
[2024-01-25 17:45:21,834] [INFO] Running command: gunzip -c /var/lib/cwl/stgfad97dd9-cd06-4391-83a2-b86d78d63151/GCF_014652775.1_ASM1465277v1_genomic.fna.gz | prodigal -d GCF_014652775.1_ASM1465277v1_genomic.fna/cds.fna -a GCF_014652775.1_ASM1465277v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 17:45:32,189] [INFO] Task succeeded: Prodigal
[2024-01-25 17:45:32,189] [INFO] Task started: HMMsearch
[2024-01-25 17:45:32,189] [INFO] Running command: hmmsearch --tblout GCF_014652775.1_ASM1465277v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2fb21fbf-7044-4ff7-b9c2-73aee3e5b824/dqc_reference/reference_markers.hmm GCF_014652775.1_ASM1465277v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 17:45:32,418] [INFO] Task succeeded: HMMsearch
[2024-01-25 17:45:32,419] [WARNING] Found 5/6 markers. [/var/lib/cwl/stgfad97dd9-cd06-4391-83a2-b86d78d63151/GCF_014652775.1_ASM1465277v1_genomic.fna.gz]
[2024-01-25 17:45:32,448] [INFO] Query marker FASTA was written to GCF_014652775.1_ASM1465277v1_genomic.fna/markers.fasta
[2024-01-25 17:45:32,448] [INFO] Task started: Blastn
[2024-01-25 17:45:32,448] [INFO] Running command: blastn -query GCF_014652775.1_ASM1465277v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2fb21fbf-7044-4ff7-b9c2-73aee3e5b824/dqc_reference/reference_markers.fasta -out GCF_014652775.1_ASM1465277v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:45:33,285] [INFO] Task succeeded: Blastn
[2024-01-25 17:45:33,288] [INFO] Selected 12 target genomes.
[2024-01-25 17:45:33,288] [INFO] Target genome list was writen to GCF_014652775.1_ASM1465277v1_genomic.fna/target_genomes.txt
[2024-01-25 17:45:33,309] [INFO] Task started: fastANI
[2024-01-25 17:45:33,309] [INFO] Running command: fastANI --query /var/lib/cwl/stgfad97dd9-cd06-4391-83a2-b86d78d63151/GCF_014652775.1_ASM1465277v1_genomic.fna.gz --refList GCF_014652775.1_ASM1465277v1_genomic.fna/target_genomes.txt --output GCF_014652775.1_ASM1465277v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 17:45:43,320] [INFO] Task succeeded: fastANI
[2024-01-25 17:45:43,320] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2fb21fbf-7044-4ff7-b9c2-73aee3e5b824/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 17:45:43,321] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2fb21fbf-7044-4ff7-b9c2-73aee3e5b824/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 17:45:43,329] [INFO] Found 12 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 17:45:43,329] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 17:45:43,329] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Thermomonas carbonis	strain=KCTC 42013	GCA_014652775.1	1463158	1463158	type	True	100.0	1137	1139	95	conclusive
Thermomonas carbonis	strain=KCTC 42013	GCA_014396975.1	1463158	1463158	type	True	99.9851	1136	1139	95	conclusive
Thermomonas aquatica	strain=SY21	GCA_006337105.1	2202149	2202149	type	True	84.8086	779	1139	95	below_threshold
Thermomonas fusca	strain=DSM 15424	GCA_000423885.1	215690	215690	type	True	82.9179	649	1139	95	below_threshold
Thermomonas haemolytica	strain=DSM 13605	GCA_004346265.1	141949	141949	type	True	82.3748	605	1139	95	below_threshold
Thermomonas haemolytica	strain=LMG 19653	GCA_006352395.1	141949	141949	type	True	82.3666	582	1139	95	below_threshold
Luteimonas aquatica	strain=RIB1-20	GCA_022662575.1	450364	450364	type	True	80.7452	585	1139	95	below_threshold
Luteimonas viscosa	strain=XBU10	GCA_008244685.1	1132694	1132694	type	True	80.6865	621	1139	95	below_threshold
Luteimonas padinae	strain=KCTC 52403	GCA_014652935.1	1714359	1714359	type	True	80.4966	518	1139	95	below_threshold
Lysobacter capsici	strain=VKM B-2533	GCA_014779555.2	435897	435897	type	True	80.2272	602	1139	95	below_threshold
Luteimonas terrae	strain=THG-MD21	GCA_004358165.1	1530191	1530191	type	True	80.1092	528	1139	95	below_threshold
Lysobacter chinensis	strain=TLK-CK17	GCA_021725675.1	2912247	2912247	type	True	80.0895	576	1139	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 17:45:43,331] [INFO] DFAST Taxonomy check result was written to GCF_014652775.1_ASM1465277v1_genomic.fna/tc_result.tsv
[2024-01-25 17:45:43,331] [INFO] ===== Taxonomy check completed =====
[2024-01-25 17:45:43,331] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 17:45:43,332] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2fb21fbf-7044-4ff7-b9c2-73aee3e5b824/dqc_reference/checkm_data
[2024-01-25 17:45:43,332] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 17:45:43,369] [INFO] Task started: CheckM
[2024-01-25 17:45:43,369] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_014652775.1_ASM1465277v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_014652775.1_ASM1465277v1_genomic.fna/checkm_input GCF_014652775.1_ASM1465277v1_genomic.fna/checkm_result
[2024-01-25 17:46:44,213] [INFO] Task succeeded: CheckM
[2024-01-25 17:46:44,214] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 17:46:44,232] [INFO] ===== Completeness check finished =====
[2024-01-25 17:46:44,232] [INFO] ===== Start GTDB Search =====
[2024-01-25 17:46:44,233] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_014652775.1_ASM1465277v1_genomic.fna/markers.fasta)
[2024-01-25 17:46:44,234] [INFO] Task started: Blastn
[2024-01-25 17:46:44,234] [INFO] Running command: blastn -query GCF_014652775.1_ASM1465277v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2fb21fbf-7044-4ff7-b9c2-73aee3e5b824/dqc_reference/reference_markers_gtdb.fasta -out GCF_014652775.1_ASM1465277v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:46:45,798] [INFO] Task succeeded: Blastn
[2024-01-25 17:46:45,801] [INFO] Selected 11 target genomes.
[2024-01-25 17:46:45,801] [INFO] Target genome list was writen to GCF_014652775.1_ASM1465277v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 17:46:45,808] [INFO] Task started: fastANI
[2024-01-25 17:46:45,809] [INFO] Running command: fastANI --query /var/lib/cwl/stgfad97dd9-cd06-4391-83a2-b86d78d63151/GCF_014652775.1_ASM1465277v1_genomic.fna.gz --refList GCF_014652775.1_ASM1465277v1_genomic.fna/target_genomes_gtdb.txt --output GCF_014652775.1_ASM1465277v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 17:46:54,162] [INFO] Task succeeded: fastANI
[2024-01-25 17:46:54,182] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 17:46:54,182] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014396975.1	s__Thermomonas carbonis	99.9851	1137	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	99.99	99.99	1.00	1.00	2	conclusive
GCF_006337105.1	s__Thermomonas sp006337105	84.8214	780	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_016720345.1	s__Thermomonas sp016720345	84.6034	693	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	99.48	99.08	0.97	0.93	11	-
GCF_011302915.1	s__Thermomonas sp011302915	83.7862	702	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017302075.1	s__Thermomonas sp017302075	83.5772	592	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014395425.1	s__Thermomonas brevis	83.2632	708	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017302095.1	s__Thermomonas sp017302095	83.2557	465	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000423885.1	s__Thermomonas fusca	82.9653	648	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	96.57	96.57	0.92	0.92	2	-
GCF_014678725.1	s__Thermomonas sp014678725	82.7033	672	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Thermomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003505795.1	s__UBA8801 sp003505795	80.8974	365	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__UBA8801	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014851915.1	s__Pseudoxanthomonas sp014851915	80.4943	570	1139	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Xanthomonadales;f__Xanthomonadaceae;g__Pseudoxanthomonas	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 17:46:54,184] [INFO] GTDB search result was written to GCF_014652775.1_ASM1465277v1_genomic.fna/result_gtdb.tsv
[2024-01-25 17:46:54,185] [INFO] ===== GTDB Search completed =====
[2024-01-25 17:46:54,188] [INFO] DFAST_QC result json was written to GCF_014652775.1_ASM1465277v1_genomic.fna/dqc_result.json
[2024-01-25 17:46:54,188] [INFO] DFAST_QC completed!
[2024-01-25 17:46:54,188] [INFO] Total running time: 0h1m33s