[2024-01-24 11:43:42,217] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:43:42,219] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:43:42,219] [INFO] DQC Reference Directory: /var/lib/cwl/stg3f72a6f5-7635-4eb8-beaa-13214339151f/dqc_reference
[2024-01-24 11:43:43,753] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:43:43,754] [INFO] Task started: Prodigal
[2024-01-24 11:43:43,754] [INFO] Running command: gunzip -c /var/lib/cwl/stgce42c13e-fb24-4088-8fc7-95017c1df28b/GCF_016127055.1_ASM1612705v1_genomic.fna.gz | prodigal -d GCF_016127055.1_ASM1612705v1_genomic.fna/cds.fna -a GCF_016127055.1_ASM1612705v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:43:48,757] [INFO] Task succeeded: Prodigal
[2024-01-24 11:43:48,758] [INFO] Task started: HMMsearch
[2024-01-24 11:43:48,758] [INFO] Running command: hmmsearch --tblout GCF_016127055.1_ASM1612705v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3f72a6f5-7635-4eb8-beaa-13214339151f/dqc_reference/reference_markers.hmm GCF_016127055.1_ASM1612705v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:43:49,056] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:43:49,057] [INFO] Found 6/6 markers.
[2024-01-24 11:43:49,079] [INFO] Query marker FASTA was written to GCF_016127055.1_ASM1612705v1_genomic.fna/markers.fasta
[2024-01-24 11:43:49,079] [INFO] Task started: Blastn
[2024-01-24 11:43:49,080] [INFO] Running command: blastn -query GCF_016127055.1_ASM1612705v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3f72a6f5-7635-4eb8-beaa-13214339151f/dqc_reference/reference_markers.fasta -out GCF_016127055.1_ASM1612705v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:43:49,681] [INFO] Task succeeded: Blastn
[2024-01-24 11:43:49,684] [INFO] Selected 15 target genomes.
[2024-01-24 11:43:49,684] [INFO] Target genome list was writen to GCF_016127055.1_ASM1612705v1_genomic.fna/target_genomes.txt
[2024-01-24 11:43:49,689] [INFO] Task started: fastANI
[2024-01-24 11:43:49,689] [INFO] Running command: fastANI --query /var/lib/cwl/stgce42c13e-fb24-4088-8fc7-95017c1df28b/GCF_016127055.1_ASM1612705v1_genomic.fna.gz --refList GCF_016127055.1_ASM1612705v1_genomic.fna/target_genomes.txt --output GCF_016127055.1_ASM1612705v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:44:00,985] [INFO] Task succeeded: fastANI
[2024-01-24 11:44:00,986] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3f72a6f5-7635-4eb8-beaa-13214339151f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:44:00,986] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3f72a6f5-7635-4eb8-beaa-13214339151f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:44:01,000] [INFO] Found 12 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 11:44:01,000] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:44:01,000] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Staphylococcus felis	strain=FDAARGOS_1014	GCA_016127055.1	46127	46127	type	True	100.0	825	826	95	conclusive
Staphylococcus felis	strain=ATCC 49168	GCA_003012915.1	46127	46127	type	True	99.9963	823	826	95	conclusive
Staphylococcus felis	strain=DSM 7377	GCA_002902185.1	46127	46127	type	True	99.8543	709	826	95	conclusive
Staphylococcus canis	strain=H16/1A	GCA_016238445.1	2724942	2724942	type	True	80.2232	410	826	95	below_threshold
Staphylococcus microti	strain=NCTC13832	GCA_900458705.1	569857	569857	type	True	79.4693	228	826	95	below_threshold
Staphylococcus schleiferi	strain=NCTC12218	GCA_900458895.2	1295	1295	type	True	79.3513	294	826	95	below_threshold
Staphylococcus pseudintermedius	strain=LMG 22219	GCA_001792775.2	283734	283734	type	True	79.1387	258	826	95	below_threshold
Staphylococcus cornubiensis	strain=NW1	GCA_900183575.1	1986155	1986155	type	True	79.0129	271	826	95	below_threshold
Staphylococcus intermedius	strain=NCTC 11048	GCA_000308095.1	1285	1285	type	True	78.803	234	826	95	below_threshold
Staphylococcus microti	strain=DSM 22147	GCA_000934465.1	569857	569857	type	True	78.7992	213	826	95	below_threshold
Staphylococcus agnetis	strain=DSM 23656	GCA_002901865.1	985762	985762	type	True	78.576	244	826	95	below_threshold
Staphylococcus shinii	strain=K22-5M	GCA_017583065.1	2912228	2912228	type	True	77.6251	153	826	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:44:01,010] [INFO] DFAST Taxonomy check result was written to GCF_016127055.1_ASM1612705v1_genomic.fna/tc_result.tsv
[2024-01-24 11:44:01,011] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:44:01,011] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:44:01,012] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3f72a6f5-7635-4eb8-beaa-13214339151f/dqc_reference/checkm_data
[2024-01-24 11:44:01,014] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:44:01,053] [INFO] Task started: CheckM
[2024-01-24 11:44:01,053] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_016127055.1_ASM1612705v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_016127055.1_ASM1612705v1_genomic.fna/checkm_input GCF_016127055.1_ASM1612705v1_genomic.fna/checkm_result
[2024-01-24 11:44:24,779] [INFO] Task succeeded: CheckM
[2024-01-24 11:44:24,780] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:44:24,800] [INFO] ===== Completeness check finished =====
[2024-01-24 11:44:24,800] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:44:24,801] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_016127055.1_ASM1612705v1_genomic.fna/markers.fasta)
[2024-01-24 11:44:24,801] [INFO] Task started: Blastn
[2024-01-24 11:44:24,801] [INFO] Running command: blastn -query GCF_016127055.1_ASM1612705v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg3f72a6f5-7635-4eb8-beaa-13214339151f/dqc_reference/reference_markers_gtdb.fasta -out GCF_016127055.1_ASM1612705v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:44:25,553] [INFO] Task succeeded: Blastn
[2024-01-24 11:44:25,556] [INFO] Selected 14 target genomes.
[2024-01-24 11:44:25,556] [INFO] Target genome list was writen to GCF_016127055.1_ASM1612705v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:44:25,566] [INFO] Task started: fastANI
[2024-01-24 11:44:25,566] [INFO] Running command: fastANI --query /var/lib/cwl/stgce42c13e-fb24-4088-8fc7-95017c1df28b/GCF_016127055.1_ASM1612705v1_genomic.fna.gz --refList GCF_016127055.1_ASM1612705v1_genomic.fna/target_genomes_gtdb.txt --output GCF_016127055.1_ASM1612705v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:44:34,828] [INFO] Task succeeded: fastANI
[2024-01-24 11:44:34,841] [INFO] Found 14 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:44:34,841] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003012915.1	s__Staphylococcus felis	99.9963	824	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.05	98.64	0.95	0.91	31	conclusive
GCF_016238445.1	s__Staphylococcus sp016238445	80.239	409	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_010365305.1	s__Staphylococcus sp010365305	79.4359	279	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900458895.2	s__Staphylococcus schleiferi	79.3293	295	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0878	99.86	99.80	0.99	0.98	25	-
GCF_002101335.1	s__Staphylococcus lutrae	79.2509	254	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.99	99.99	1.00	1.00	2	-
GCF_001792775.2	s__Staphylococcus pseudintermedius	79.1481	260	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.31	98.36	0.94	0.88	431	-
GCF_002902385.1	s__Staphylococcus intermedius	79.1295	246	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.80	99.54	0.96	0.94	8	-
GCF_900183575.1	s__Staphylococcus cornubiensis	79.0051	270	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003491325.1	s__Staphylococcus warneri_A	78.8935	172	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.32	98.95	0.94	0.90	22	-
GCF_000934465.1	s__Staphylococcus microti	78.8027	212	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.99	99.97	0.98	0.96	3	-
GCF_900636385.1	s__Staphylococcus warneri	78.6225	169	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.98	95.90	0.95	0.90	73	-
GCF_002901995.1	s__Staphylococcus coagulans	78.5832	277	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0878	98.48	97.57	0.97	0.93	43	-
GCF_002901865.1	s__Staphylococcus agnetis	78.5576	245	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.49	96.84	0.93	0.88	40	-
GCF_002902365.1	s__Staphylococcus cohnii	77.9038	158	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	97.14	95.73	0.92	0.88	59	-
--------------------------------------------------------------------------------
[2024-01-24 11:44:34,843] [INFO] GTDB search result was written to GCF_016127055.1_ASM1612705v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:44:34,844] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:44:34,850] [INFO] DFAST_QC result json was written to GCF_016127055.1_ASM1612705v1_genomic.fna/dqc_result.json
[2024-01-24 11:44:34,850] [INFO] DFAST_QC completed!
[2024-01-24 11:44:34,851] [INFO] Total running time: 0h0m53s