[2024-01-24 12:14:49,287] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:14:49,289] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:14:49,289] [INFO] DQC Reference Directory: /var/lib/cwl/stg02f1bd9b-737e-41b5-95ec-6ed091cc312d/dqc_reference
[2024-01-24 12:14:50,696] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:14:50,697] [INFO] Task started: Prodigal
[2024-01-24 12:14:50,697] [INFO] Running command: gunzip -c /var/lib/cwl/stg10b93f07-a694-4c79-9f1d-440975a489f8/GCF_016623605.1_ASM1662360v1_genomic.fna.gz | prodigal -d GCF_016623605.1_ASM1662360v1_genomic.fna/cds.fna -a GCF_016623605.1_ASM1662360v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:14:59,526] [INFO] Task succeeded: Prodigal
[2024-01-24 12:14:59,526] [INFO] Task started: HMMsearch
[2024-01-24 12:14:59,527] [INFO] Running command: hmmsearch --tblout GCF_016623605.1_ASM1662360v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg02f1bd9b-737e-41b5-95ec-6ed091cc312d/dqc_reference/reference_markers.hmm GCF_016623605.1_ASM1662360v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:14:59,784] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:14:59,786] [INFO] Found 6/6 markers.
[2024-01-24 12:14:59,814] [INFO] Query marker FASTA was written to GCF_016623605.1_ASM1662360v1_genomic.fna/markers.fasta
[2024-01-24 12:14:59,815] [INFO] Task started: Blastn
[2024-01-24 12:14:59,815] [INFO] Running command: blastn -query GCF_016623605.1_ASM1662360v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg02f1bd9b-737e-41b5-95ec-6ed091cc312d/dqc_reference/reference_markers.fasta -out GCF_016623605.1_ASM1662360v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:15:00,580] [INFO] Task succeeded: Blastn
[2024-01-24 12:15:00,585] [INFO] Selected 16 target genomes.
[2024-01-24 12:15:00,586] [INFO] Target genome list was writen to GCF_016623605.1_ASM1662360v1_genomic.fna/target_genomes.txt
[2024-01-24 12:15:00,597] [INFO] Task started: fastANI
[2024-01-24 12:15:00,598] [INFO] Running command: fastANI --query /var/lib/cwl/stg10b93f07-a694-4c79-9f1d-440975a489f8/GCF_016623605.1_ASM1662360v1_genomic.fna.gz --refList GCF_016623605.1_ASM1662360v1_genomic.fna/target_genomes.txt --output GCF_016623605.1_ASM1662360v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:15:14,354] [INFO] Task succeeded: fastANI
[2024-01-24 12:15:14,355] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg02f1bd9b-737e-41b5-95ec-6ed091cc312d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:15:14,355] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg02f1bd9b-737e-41b5-95ec-6ed091cc312d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:15:14,368] [INFO] Found 16 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 12:15:14,369] [INFO] The taxonomy check result is classified as 'inconclusive'.
[2024-01-24 12:15:14,369] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Kingella bonacorsii	strain=Marseille-Q4569	GCA_016623605.1	2796361	2796361	type	True	100.0	825	829	95	inconclusive
Kingella oralis	strain=DSM 18271	GCA_014054985.1	505	505	type	True	96.4452	729	829	95	inconclusive
Kingella oralis	strain=ATCC 51147	GCA_000160435.1	505	505	type	True	96.44	723	829	95	inconclusive
Neisseria bacilliformis	strain=DSM 23338	GCA_014055025.1	267212	267212	type	True	79.9026	321	829	95	below_threshold
Neisseria bacilliformis	strain=ATCC BAA-1200	GCA_000194925.1	267212	267212	type	True	79.776	304	829	95	below_threshold
Kingella denitrificans	strain=FDAARGOS_1060	GCA_016127355.1	502	502	type	True	79.5054	345	829	95	below_threshold
Kingella denitrificans	strain=ATCC 33394	GCA_000190695.1	502	502	type	True	79.2673	333	829	95	below_threshold
Kingella potus	strain=NCTC13336	GCA_900451175.1	265175	265175	type	True	78.9142	267	829	95	below_threshold
Eikenella longinqua	strain=NML02-A-017	GCA_001648355.1	1795827	1795827	type	True	78.4214	247	829	95	below_threshold
Kingella kingae	strain=4177/66	GCA_022871005.1	504	504	type	True	78.3749	245	829	95	below_threshold
Neisseria dumasiana	strain=LMG 30012	GCA_022870885.1	1931275	1931275	type	True	78.1377	217	829	95	below_threshold
Alysiella crassa	strain=S6	GCA_022871045.1	153491	153491	type	True	78.0406	292	829	95	below_threshold
Uruburuella suis	strain=1258/02	GCA_022870845.1	252130	252130	type	True	77.8841	256	829	95	below_threshold
Alysiella crassa	strain=DSM 2578	GCA_000745955.1	153491	153491	type	True	77.7751	270	829	95	below_threshold
Uruburuella testudinis	strain=CCUG 63373m	GCA_022870865.1	1282863	1282863	type	True	77.6176	217	829	95	below_threshold
Uruburuella suis	strain=DSM 17474	GCA_004341385.1	252130	252130	type	True	77.5942	241	829	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:15:14,372] [INFO] DFAST Taxonomy check result was written to GCF_016623605.1_ASM1662360v1_genomic.fna/tc_result.tsv
[2024-01-24 12:15:14,372] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:15:14,372] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:15:14,373] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg02f1bd9b-737e-41b5-95ec-6ed091cc312d/dqc_reference/checkm_data
[2024-01-24 12:15:14,374] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:15:14,408] [INFO] Task started: CheckM
[2024-01-24 12:15:14,408] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_016623605.1_ASM1662360v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_016623605.1_ASM1662360v1_genomic.fna/checkm_input GCF_016623605.1_ASM1662360v1_genomic.fna/checkm_result
[2024-01-24 12:15:43,879] [INFO] Task succeeded: CheckM
[2024-01-24 12:15:43,880] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 96.12%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:15:43,899] [INFO] ===== Completeness check finished =====
[2024-01-24 12:15:43,900] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:15:43,900] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_016623605.1_ASM1662360v1_genomic.fna/markers.fasta)
[2024-01-24 12:15:43,901] [INFO] Task started: Blastn
[2024-01-24 12:15:43,901] [INFO] Running command: blastn -query GCF_016623605.1_ASM1662360v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg02f1bd9b-737e-41b5-95ec-6ed091cc312d/dqc_reference/reference_markers_gtdb.fasta -out GCF_016623605.1_ASM1662360v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:15:45,112] [INFO] Task succeeded: Blastn
[2024-01-24 12:15:45,116] [INFO] Selected 19 target genomes.
[2024-01-24 12:15:45,117] [INFO] Target genome list was writen to GCF_016623605.1_ASM1662360v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:15:45,129] [INFO] Task started: fastANI
[2024-01-24 12:15:45,129] [INFO] Running command: fastANI --query /var/lib/cwl/stg10b93f07-a694-4c79-9f1d-440975a489f8/GCF_016623605.1_ASM1662360v1_genomic.fna.gz --refList GCF_016623605.1_ASM1662360v1_genomic.fna/target_genomes_gtdb.txt --output GCF_016623605.1_ASM1662360v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:15:56,850] [INFO] Task succeeded: fastANI
[2024-01-24 12:15:56,866] [INFO] Found 19 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:15:56,866] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014054985.1	s__Kingella_B oralis	96.4582	732	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Kingella_B	95.0	98.22	96.45	0.95	0.91	3	conclusive
GCA_905373195.1	s__Kingella_B oralis_A	91.4674	345	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Kingella_B	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014055025.1	s__Neisseria bacilliformis	79.8693	321	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	98.33	96.96	0.94	0.89	4	-
GCF_000190695.1	s__Kingella_A denitrificans	79.2673	333	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Kingella_A	95.0	98.63	96.04	0.97	0.93	4	-
GCF_003990355.1	s__Eikenella corrodens_B	79.2663	246	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Eikenella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900187105.1	s__Eikenella corrodens	79.0819	223	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Eikenella	95.0	96.96	95.30	0.90	0.87	23	-
GCF_002237445.1	s__Neisseria sp002237445	78.9054	258	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	99.69	99.69	0.98	0.98	2	-
GCF_900451175.1	s__Neisseria potus	78.8892	267	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001648475.1	s__Eikenella halliae	78.8775	240	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Eikenella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900636765.1	s__Neisseria canis	78.7317	176	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	99.98	99.98	1.00	1.00	2	-
GCF_005886145.1	s__Neisseria sp000090875	78.5083	244	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	98.69	97.41	0.97	0.94	3	-
GCF_001648355.1	s__Eikenella longinqua	78.4272	247	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Eikenella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016025525.2	s__Eikenella sp016025525	78.3829	245	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Eikenella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002216145.1	s__Neisseria chenwenguii	78.2755	248	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	99.73	99.73	0.96	0.96	2	-
GCF_014054525.1	s__Alysiella filiformis	78.1819	290	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Alysiella	95.0	99.98	99.98	1.00	1.00	2	-
GCF_008807015.1	s__Neisseria zalophi	78.073	96	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000751855.1	s__Kingella negevensis	77.9747	280	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Kingella	95.0	99.09	98.48	0.96	0.94	3	-
GCF_002108505.1	s__Neisseria dumasiana	77.8353	209	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	97.54	97.26	0.91	0.89	4	-
GCF_000745955.1	s__Alysiella crassa	77.7704	272	829	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Alysiella	95.0	99.94	99.94	0.99	0.99	2	-
--------------------------------------------------------------------------------
[2024-01-24 12:15:56,868] [INFO] GTDB search result was written to GCF_016623605.1_ASM1662360v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:15:56,868] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:15:56,873] [INFO] DFAST_QC result json was written to GCF_016623605.1_ASM1662360v1_genomic.fna/dqc_result.json
[2024-01-24 12:15:56,873] [INFO] DFAST_QC completed!
[2024-01-24 12:15:56,873] [INFO] Total running time: 0h1m8s