[2024-01-24 14:14:29,491] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:14:29,492] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:14:29,493] [INFO] DQC Reference Directory: /var/lib/cwl/stg6f6495db-6261-49e2-a2ba-49f7db679953/dqc_reference
[2024-01-24 14:14:32,433] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:14:32,435] [INFO] Task started: Prodigal
[2024-01-24 14:14:32,436] [INFO] Running command: gunzip -c /var/lib/cwl/stgce31bccf-20cd-42c1-bf7b-9ad8c8c4a8f7/GCF_016653375.1_ASM1665337v1_genomic.fna.gz | prodigal -d GCF_016653375.1_ASM1665337v1_genomic.fna/cds.fna -a GCF_016653375.1_ASM1665337v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:14:47,075] [INFO] Task succeeded: Prodigal
[2024-01-24 14:14:47,076] [INFO] Task started: HMMsearch
[2024-01-24 14:14:47,076] [INFO] Running command: hmmsearch --tblout GCF_016653375.1_ASM1665337v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6f6495db-6261-49e2-a2ba-49f7db679953/dqc_reference/reference_markers.hmm GCF_016653375.1_ASM1665337v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:14:47,453] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:14:47,454] [INFO] Found 6/6 markers.
[2024-01-24 14:14:47,515] [INFO] Query marker FASTA was written to GCF_016653375.1_ASM1665337v1_genomic.fna/markers.fasta
[2024-01-24 14:14:47,516] [INFO] Task started: Blastn
[2024-01-24 14:14:47,516] [INFO] Running command: blastn -query GCF_016653375.1_ASM1665337v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6f6495db-6261-49e2-a2ba-49f7db679953/dqc_reference/reference_markers.fasta -out GCF_016653375.1_ASM1665337v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:14:49,435] [INFO] Task succeeded: Blastn
[2024-01-24 14:14:49,439] [INFO] Selected 20 target genomes.
[2024-01-24 14:14:49,440] [INFO] Target genome list was writen to GCF_016653375.1_ASM1665337v1_genomic.fna/target_genomes.txt
[2024-01-24 14:14:49,454] [INFO] Task started: fastANI
[2024-01-24 14:14:49,454] [INFO] Running command: fastANI --query /var/lib/cwl/stgce31bccf-20cd-42c1-bf7b-9ad8c8c4a8f7/GCF_016653375.1_ASM1665337v1_genomic.fna.gz --refList GCF_016653375.1_ASM1665337v1_genomic.fna/target_genomes.txt --output GCF_016653375.1_ASM1665337v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:15:13,229] [INFO] Task succeeded: fastANI
[2024-01-24 14:15:13,231] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6f6495db-6261-49e2-a2ba-49f7db679953/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:15:13,231] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6f6495db-6261-49e2-a2ba-49f7db679953/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:15:13,254] [INFO] Found 20 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 14:15:13,255] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:15:13,255] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Rhodobium orientis	strain=DSM 11290	GCA_016653375.1	34017	34017	type	True	100.0	1675	1676	95	conclusive
Rhodobium orientis	strain=DSM 11290	GCA_014197785.1	34017	34017	type	True	99.9965	1650	1676	95	conclusive
Rhodobium orientis	strain=DSM 11290	GCA_003258835.1	34017	34017	type	True	99.9719	1617	1676	95	conclusive
Oharaeibacter diazotrophicus	strain=SM30	GCA_011317485.1	1920512	1920512	type	True	78.36	465	1676	95	below_threshold
Oharaeibacter diazotrophicus	strain=DSM 102969	GCA_004362745.1	1920512	1920512	type	True	78.075	531	1676	95	below_threshold
Shinella pollutisoli	strain=KCTC 52677	GCA_024609765.1	2250594	2250594	type	True	78.0635	481	1676	95	below_threshold
Stappia albiluteola	strain=F7233	GCA_014050225.1	2758565	2758565	type	True	78.0056	403	1676	95	below_threshold
Afifella pfennigii	strain=DSM 17143	GCA_000688515.1	209897	209897	type	True	77.9419	436	1676	95	below_threshold
Kaistia adipata	strain=DSM 17808	GCA_000423225.1	166954	166954	type	True	77.8272	436	1676	95	below_threshold
Roseibium aestuarii	strain=SYSU M00256-3	GCA_008477545.1	2600299	2600299	type	True	77.8049	359	1676	95	below_threshold
Rhodoplanes piscinae	strain=DSM 19946	GCA_003258855.1	444923	444923	type	True	77.7941	388	1676	95	below_threshold
Chthonobacter albigriseus	strain=KCTC 42450	GCA_013839445.1	1683161	1683161	type	True	77.7862	404	1676	95	below_threshold
Jiella sonneratiae	strain=MQZ13P-4	GCA_017353515.1	2816856	2816856	type	True	77.7781	470	1676	95	below_threshold
Chelatococcus caeni	strain=DSM 103737	GCA_014196925.1	1348468	1348468	type	True	77.5546	471	1676	95	below_threshold
Oricola indica	strain=JL-62	GCA_019966595.1	2872591	2872591	type	True	77.4446	301	1676	95	below_threshold
Methylobacterium nonmethylotrophicum	strain=6HR-1	GCA_004745635.1	1141884	1141884	type	True	77.402	430	1676	95	below_threshold
Mesorhizobium comanense	strain=3P27G6	GCA_005503535.1	2502215	2502215	type	True	77.4008	400	1676	95	below_threshold
Methylobacterium terricola	strain=17Sr1-39	GCA_006151805.1	2583531	2583531	type	True	77.321	438	1676	95	below_threshold
Phreatobacter stygius	strain=KCTC 52518	GCA_005144885.1	1940610	1940610	type	True	77.0394	410	1676	95	below_threshold
Rhodoligotrophos defluvii	strain=lm1	GCA_005281615.1	2561934	2561934	type	True	76.9947	248	1676	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:15:13,257] [INFO] DFAST Taxonomy check result was written to GCF_016653375.1_ASM1665337v1_genomic.fna/tc_result.tsv
[2024-01-24 14:15:13,257] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:15:13,258] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:15:13,258] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6f6495db-6261-49e2-a2ba-49f7db679953/dqc_reference/checkm_data
[2024-01-24 14:15:13,259] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:15:13,321] [INFO] Task started: CheckM
[2024-01-24 14:15:13,321] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_016653375.1_ASM1665337v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_016653375.1_ASM1665337v1_genomic.fna/checkm_input GCF_016653375.1_ASM1665337v1_genomic.fna/checkm_result
[2024-01-24 14:15:59,906] [INFO] Task succeeded: CheckM
[2024-01-24 14:15:59,909] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:15:59,929] [INFO] ===== Completeness check finished =====
[2024-01-24 14:15:59,930] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:15:59,930] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_016653375.1_ASM1665337v1_genomic.fna/markers.fasta)
[2024-01-24 14:15:59,931] [INFO] Task started: Blastn
[2024-01-24 14:15:59,931] [INFO] Running command: blastn -query GCF_016653375.1_ASM1665337v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6f6495db-6261-49e2-a2ba-49f7db679953/dqc_reference/reference_markers_gtdb.fasta -out GCF_016653375.1_ASM1665337v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:16:04,952] [INFO] Task succeeded: Blastn
[2024-01-24 14:16:04,961] [INFO] Selected 22 target genomes.
[2024-01-24 14:16:04,961] [INFO] Target genome list was writen to GCF_016653375.1_ASM1665337v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:16:04,982] [INFO] Task started: fastANI
[2024-01-24 14:16:04,982] [INFO] Running command: fastANI --query /var/lib/cwl/stgce31bccf-20cd-42c1-bf7b-9ad8c8c4a8f7/GCF_016653375.1_ASM1665337v1_genomic.fna.gz --refList GCF_016653375.1_ASM1665337v1_genomic.fna/target_genomes_gtdb.txt --output GCF_016653375.1_ASM1665337v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:16:28,252] [INFO] Task succeeded: fastANI
[2024-01-24 14:16:28,314] [INFO] Found 22 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:16:28,314] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003258835.1	s__Rhodobium orientis	99.9719	1617	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhodobiaceae;g__Rhodobium	95.0	99.99	99.98	0.99	0.98	3	conclusive
GCA_002869065.1	s__Rhodobium sp002869065	79.3055	650	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhodobiaceae;g__Rhodobium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900185725.1	s__Stappia sp900185725	78.7476	557	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Stappiaceae;g__Stappia	95.0	98.18	97.99	0.94	0.94	3	-
GCF_000192745.1	s__Polymorphum gilvum	78.7392	533	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Stappiaceae;g__Polymorphum	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003610575.1	s__Stappia sp003610575	78.5927	519	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Stappiaceae;g__Stappia	95.0	98.18	98.18	0.97	0.97	2	-
GCF_001696535.1	s__Stappia indica	78.5867	546	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Stappiaceae;g__Stappia	95.0	97.23	96.79	0.94	0.94	3	-
GCF_015904235.1	s__L22 sp015904235	78.0758	523	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Pleomorphomonadaceae;g__L22	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004362745.1	s__Oharaeibacter diazotrophicus	78.0472	529	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Pleomorphomonadaceae;g__Oharaeibacter	95.0	99.98	99.97	1.00	1.00	3	-
GCA_017305835.1	s__RCIO01 sp017305835	78.0458	471	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__RCIO01	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000688515.1	s__Afifella pfennigii	77.9346	437	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Afifellaceae;g__Afifella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013839525.1	s__Mongoliimonas rhizosphaerae	77.8932	431	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Pleomorphomonadaceae;g__Mongoliimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014199915.1	s__Prosthecomicrobium pneumaticum	77.8413	442	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Kaistiaceae;g__Prosthecomicrobium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001305515.1	s__Prosthecomicrobium_A hirschii	77.8018	563	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Ancalomicrobiaceae;g__Prosthecomicrobium_A	95.0	97.89	97.89	0.95	0.95	2	-
GCF_013839445.1	s__Chthonobacter albigriseus	77.7705	406	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Pleomorphomonadaceae;g__Chthonobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016629525.1	s__Kaistia sp016629525	77.7291	446	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Kaistiaceae;g__Kaistia	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017305815.1	s__Kaistia sp017305815	77.6278	290	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Kaistiaceae;g__Kaistia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_006442795.1	s__Mesorhizobium sp006442795	77.5738	406	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Mesorhizobium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014196925.1	s__Chelatococcus_A caeni	77.5659	470	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Chelatococcus_A	95.0	98.75	98.72	0.96	0.95	3	-
GCA_017307955.1	s__Kaistia sp017307955	77.5112	437	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Kaistiaceae;g__Kaistia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016804325.1	s__Methylobacterium aquaticum_C	77.4743	428	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004745635.1	s__Methylobacterium sp004745635	77.3965	430	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_005144885.1	s__Phreatobacter stygius	77.0877	405	1676	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Phreatobacteraceae;g__Phreatobacter	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 14:16:28,318] [INFO] GTDB search result was written to GCF_016653375.1_ASM1665337v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:16:28,323] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:16:28,330] [INFO] DFAST_QC result json was written to GCF_016653375.1_ASM1665337v1_genomic.fna/dqc_result.json
[2024-01-24 14:16:28,331] [INFO] DFAST_QC completed!
[2024-01-24 14:16:28,331] [INFO] Total running time: 0h1m59s