[2024-01-25 17:50:50,609] [INFO] DFAST_QC pipeline started.
[2024-01-25 17:50:50,611] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 17:50:50,611] [INFO] DQC Reference Directory: /var/lib/cwl/stg6181c295-f351-40ef-a47f-915b7da02b7c/dqc_reference
[2024-01-25 17:50:51,720] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 17:50:51,721] [INFO] Task started: Prodigal
[2024-01-25 17:50:51,721] [INFO] Running command: gunzip -c /var/lib/cwl/stg4166db57-68f3-45fe-bdf1-fd6141aa0c73/GCF_016722965.1_ASM1672296v1_genomic.fna.gz | prodigal -d GCF_016722965.1_ASM1672296v1_genomic.fna/cds.fna -a GCF_016722965.1_ASM1672296v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 17:51:15,558] [INFO] Task succeeded: Prodigal
[2024-01-25 17:51:15,558] [INFO] Task started: HMMsearch
[2024-01-25 17:51:15,558] [INFO] Running command: hmmsearch --tblout GCF_016722965.1_ASM1672296v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6181c295-f351-40ef-a47f-915b7da02b7c/dqc_reference/reference_markers.hmm GCF_016722965.1_ASM1672296v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 17:51:15,814] [INFO] Task succeeded: HMMsearch
[2024-01-25 17:51:15,815] [INFO] Found 6/6 markers.
[2024-01-25 17:51:15,853] [INFO] Query marker FASTA was written to GCF_016722965.1_ASM1672296v1_genomic.fna/markers.fasta
[2024-01-25 17:51:15,853] [INFO] Task started: Blastn
[2024-01-25 17:51:15,853] [INFO] Running command: blastn -query GCF_016722965.1_ASM1672296v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6181c295-f351-40ef-a47f-915b7da02b7c/dqc_reference/reference_markers.fasta -out GCF_016722965.1_ASM1672296v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:51:16,399] [INFO] Task succeeded: Blastn
[2024-01-25 17:51:16,402] [INFO] Selected 16 target genomes.
[2024-01-25 17:51:16,402] [INFO] Target genome list was writen to GCF_016722965.1_ASM1672296v1_genomic.fna/target_genomes.txt
[2024-01-25 17:51:16,420] [INFO] Task started: fastANI
[2024-01-25 17:51:16,420] [INFO] Running command: fastANI --query /var/lib/cwl/stg4166db57-68f3-45fe-bdf1-fd6141aa0c73/GCF_016722965.1_ASM1672296v1_genomic.fna.gz --refList GCF_016722965.1_ASM1672296v1_genomic.fna/target_genomes.txt --output GCF_016722965.1_ASM1672296v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 17:51:33,007] [INFO] Task succeeded: fastANI
[2024-01-25 17:51:33,007] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6181c295-f351-40ef-a47f-915b7da02b7c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 17:51:33,007] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6181c295-f351-40ef-a47f-915b7da02b7c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 17:51:33,013] [INFO] Found 6 fastANI hits (1 hits with ANI > threshold)
[2024-01-25 17:51:33,013] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 17:51:33,013] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Tumebacillus amylolyticus	strain=ITR2	GCA_016722965.1	2801339	2801339	type	True	100.0	1587	1587	95	conclusive
Tumebacillus flagellatus	strain=GST4	GCA_000714935.1	1157490	1157490	type	True	83.7562	1100	1587	95	below_threshold
Tumebacillus permanentifrigoris	strain=DSM 18773	GCA_003148565.1	378543	378543	type	True	81.4595	919	1587	95	below_threshold
Tumebacillus avium	strain=AR23208	GCA_002162355.1	1903704	1903704	type	True	78.0304	425	1587	95	below_threshold
Tumebacillus algifaecis	strain=THMBR28	GCA_002243515.1	1214604	1214604	type	True	77.6457	277	1587	95	below_threshold
Paenibacillus artemisiicola	strain=MWE-103	GCA_017652985.1	1172618	1172618	type	True	75.7574	66	1587	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 17:51:33,015] [INFO] DFAST Taxonomy check result was written to GCF_016722965.1_ASM1672296v1_genomic.fna/tc_result.tsv
[2024-01-25 17:51:33,016] [INFO] ===== Taxonomy check completed =====
[2024-01-25 17:51:33,016] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 17:51:33,016] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6181c295-f351-40ef-a47f-915b7da02b7c/dqc_reference/checkm_data
[2024-01-25 17:51:33,017] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 17:51:33,063] [INFO] Task started: CheckM
[2024-01-25 17:51:33,064] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_016722965.1_ASM1672296v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_016722965.1_ASM1672296v1_genomic.fna/checkm_input GCF_016722965.1_ASM1672296v1_genomic.fna/checkm_result
[2024-01-25 17:52:40,214] [INFO] Task succeeded: CheckM
[2024-01-25 17:52:40,215] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 17:52:40,237] [INFO] ===== Completeness check finished =====
[2024-01-25 17:52:40,237] [INFO] ===== Start GTDB Search =====
[2024-01-25 17:52:40,238] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_016722965.1_ASM1672296v1_genomic.fna/markers.fasta)
[2024-01-25 17:52:40,238] [INFO] Task started: Blastn
[2024-01-25 17:52:40,238] [INFO] Running command: blastn -query GCF_016722965.1_ASM1672296v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6181c295-f351-40ef-a47f-915b7da02b7c/dqc_reference/reference_markers_gtdb.fasta -out GCF_016722965.1_ASM1672296v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 17:52:41,038] [INFO] Task succeeded: Blastn
[2024-01-25 17:52:41,041] [INFO] Selected 15 target genomes.
[2024-01-25 17:52:41,041] [INFO] Target genome list was writen to GCF_016722965.1_ASM1672296v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 17:52:41,057] [INFO] Task started: fastANI
[2024-01-25 17:52:41,057] [INFO] Running command: fastANI --query /var/lib/cwl/stg4166db57-68f3-45fe-bdf1-fd6141aa0c73/GCF_016722965.1_ASM1672296v1_genomic.fna.gz --refList GCF_016722965.1_ASM1672296v1_genomic.fna/target_genomes_gtdb.txt --output GCF_016722965.1_ASM1672296v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 17:52:53,805] [INFO] Task succeeded: fastANI
[2024-01-25 17:52:53,811] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 17:52:53,811] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016722965.1	s__Tumebacillus sp016722965	100.0	1587	1587	d__Bacteria;p__Firmicutes;c__Bacilli;o__Tumebacillales;f__Tumebacillaceae;g__Tumebacillus	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_000714935.1	s__Tumebacillus flagellatus	83.7562	1100	1587	d__Bacteria;p__Firmicutes;c__Bacilli;o__Tumebacillales;f__Tumebacillaceae;g__Tumebacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003148565.1	s__Tumebacillus permanentifrigoris	81.4472	921	1587	d__Bacteria;p__Firmicutes;c__Bacilli;o__Tumebacillales;f__Tumebacillaceae;g__Tumebacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004340785.1	s__Tumebacillus_A sp004340785	78.2944	379	1587	d__Bacteria;p__Firmicutes;c__Bacilli;o__Tumebacillales;f__Tumebacillaceae;g__Tumebacillus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002162355.1	s__Tumebacillus_A avium	78.0598	421	1587	d__Bacteria;p__Firmicutes;c__Bacilli;o__Tumebacillales;f__Tumebacillaceae;g__Tumebacillus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002243515.1	s__Tumebacillus_A algifaecis	77.6493	278	1587	d__Bacteria;p__Firmicutes;c__Bacilli;o__Tumebacillales;f__Tumebacillaceae;g__Tumebacillus_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900113915.1	s__Paenibacillus_Z sp900113915	75.9152	64	1587	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Paenibacillus_Z	95.0	96.39	96.39	0.86	0.86	2	-
--------------------------------------------------------------------------------
[2024-01-25 17:52:53,814] [INFO] GTDB search result was written to GCF_016722965.1_ASM1672296v1_genomic.fna/result_gtdb.tsv
[2024-01-25 17:52:53,815] [INFO] ===== GTDB Search completed =====
[2024-01-25 17:52:53,817] [INFO] DFAST_QC result json was written to GCF_016722965.1_ASM1672296v1_genomic.fna/dqc_result.json
[2024-01-25 17:52:53,817] [INFO] DFAST_QC completed!
[2024-01-25 17:52:53,817] [INFO] Total running time: 0h2m3s