[2024-01-24 12:36:58,629] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:36:58,631] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:36:58,631] [INFO] DQC Reference Directory: /var/lib/cwl/stgbbe5163c-69c5-4545-b6a3-be84acf5734d/dqc_reference
[2024-01-24 12:37:00,092] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:37:00,093] [INFO] Task started: Prodigal
[2024-01-24 12:37:00,093] [INFO] Running command: gunzip -c /var/lib/cwl/stgcc2a63c8-8fa6-4ce5-893a-a4cdd024e9fe/GCF_016757965.1_ASM1675796v1_genomic.fna.gz | prodigal -d GCF_016757965.1_ASM1675796v1_genomic.fna/cds.fna -a GCF_016757965.1_ASM1675796v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:37:05,373] [INFO] Task succeeded: Prodigal
[2024-01-24 12:37:05,373] [INFO] Task started: HMMsearch
[2024-01-24 12:37:05,373] [INFO] Running command: hmmsearch --tblout GCF_016757965.1_ASM1675796v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgbbe5163c-69c5-4545-b6a3-be84acf5734d/dqc_reference/reference_markers.hmm GCF_016757965.1_ASM1675796v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:37:05,620] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:37:05,621] [WARNING] Found 3/6 markers. [/var/lib/cwl/stgcc2a63c8-8fa6-4ce5-893a-a4cdd024e9fe/GCF_016757965.1_ASM1675796v1_genomic.fna.gz]
[2024-01-24 12:37:05,662] [INFO] Query marker FASTA was written to GCF_016757965.1_ASM1675796v1_genomic.fna/markers.fasta
[2024-01-24 12:37:05,662] [INFO] Task started: Blastn
[2024-01-24 12:37:05,662] [INFO] Running command: blastn -query GCF_016757965.1_ASM1675796v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbbe5163c-69c5-4545-b6a3-be84acf5734d/dqc_reference/reference_markers.fasta -out GCF_016757965.1_ASM1675796v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:37:06,174] [INFO] Task succeeded: Blastn
[2024-01-24 12:37:06,191] [INFO] Selected 6 target genomes.
[2024-01-24 12:37:06,191] [INFO] Target genome list was writen to GCF_016757965.1_ASM1675796v1_genomic.fna/target_genomes.txt
[2024-01-24 12:37:06,198] [INFO] Task started: fastANI
[2024-01-24 12:37:06,198] [INFO] Running command: fastANI --query /var/lib/cwl/stgcc2a63c8-8fa6-4ce5-893a-a4cdd024e9fe/GCF_016757965.1_ASM1675796v1_genomic.fna.gz --refList GCF_016757965.1_ASM1675796v1_genomic.fna/target_genomes.txt --output GCF_016757965.1_ASM1675796v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:37:09,367] [INFO] Task succeeded: fastANI
[2024-01-24 12:37:09,367] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgbbe5163c-69c5-4545-b6a3-be84acf5734d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:37:09,368] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgbbe5163c-69c5-4545-b6a3-be84acf5734d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:37:09,371] [INFO] Found 1 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 12:37:09,371] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:37:09,371] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Archaeoglobus neptunius	strain=SE56	GCA_016757965.1	2798580	2798580	type	True	100.0	694	694	95	conclusive
--------------------------------------------------------------------------------
[2024-01-24 12:37:09,373] [INFO] DFAST Taxonomy check result was written to GCF_016757965.1_ASM1675796v1_genomic.fna/tc_result.tsv
[2024-01-24 12:37:09,373] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:37:09,374] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:37:09,374] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgbbe5163c-69c5-4545-b6a3-be84acf5734d/dqc_reference/checkm_data
[2024-01-24 12:37:09,375] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:37:09,402] [INFO] Task started: CheckM
[2024-01-24 12:37:09,402] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_016757965.1_ASM1675796v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_016757965.1_ASM1675796v1_genomic.fna/checkm_input GCF_016757965.1_ASM1675796v1_genomic.fna/checkm_result
[2024-01-24 12:37:39,510] [INFO] Task succeeded: CheckM
[2024-01-24 12:37:39,511] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:37:39,545] [INFO] ===== Completeness check finished =====
[2024-01-24 12:37:39,546] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:37:39,546] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_016757965.1_ASM1675796v1_genomic.fna/markers.fasta)
[2024-01-24 12:37:39,547] [INFO] Task started: Blastn
[2024-01-24 12:37:39,547] [INFO] Running command: blastn -query GCF_016757965.1_ASM1675796v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgbbe5163c-69c5-4545-b6a3-be84acf5734d/dqc_reference/reference_markers_gtdb.fasta -out GCF_016757965.1_ASM1675796v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:37:39,996] [INFO] Task succeeded: Blastn
[2024-01-24 12:37:39,999] [INFO] Selected 9 target genomes.
[2024-01-24 12:37:40,000] [INFO] Target genome list was writen to GCF_016757965.1_ASM1675796v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:37:40,031] [INFO] Task started: fastANI
[2024-01-24 12:37:40,031] [INFO] Running command: fastANI --query /var/lib/cwl/stgcc2a63c8-8fa6-4ce5-893a-a4cdd024e9fe/GCF_016757965.1_ASM1675796v1_genomic.fna.gz --refList GCF_016757965.1_ASM1675796v1_genomic.fna/target_genomes_gtdb.txt --output GCF_016757965.1_ASM1675796v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:37:43,979] [INFO] Task succeeded: fastANI
[2024-01-24 12:37:43,983] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:37:43,983] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016757965.1	s__Archaeoglobus sp016757965	100.0	694	694	d__Archaea;p__Halobacteriota;c__Archaeoglobi;o__Archaeoglobales;f__Archaeoglobaceae;g__Archaeoglobus	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_017656475.1	s__Archaeoglobus sp017656475	77.7118	127	694	d__Archaea;p__Halobacteriota;c__Archaeoglobi;o__Archaeoglobales;f__Archaeoglobaceae;g__Archaeoglobus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:37:43,985] [INFO] GTDB search result was written to GCF_016757965.1_ASM1675796v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:37:43,985] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:37:43,987] [INFO] DFAST_QC result json was written to GCF_016757965.1_ASM1675796v1_genomic.fna/dqc_result.json
[2024-01-24 12:37:43,987] [INFO] DFAST_QC completed!
[2024-01-24 12:37:43,987] [INFO] Total running time: 0h0m45s