[2024-01-24 12:36:50,185] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:36:50,188] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:36:50,188] [INFO] DQC Reference Directory: /var/lib/cwl/stg970ce83c-61c9-4d95-a548-5b3c9fe9e7b2/dqc_reference
[2024-01-24 12:36:52,578] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:36:52,579] [INFO] Task started: Prodigal
[2024-01-24 12:36:52,579] [INFO] Running command: gunzip -c /var/lib/cwl/stge4611906-35f4-44ee-b0b8-3efb39a9017d/GCF_016765635.1_ASM1676563v1_genomic.fna.gz | prodigal -d GCF_016765635.1_ASM1676563v1_genomic.fna/cds.fna -a GCF_016765635.1_ASM1676563v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:37:02,970] [INFO] Task succeeded: Prodigal
[2024-01-24 12:37:02,970] [INFO] Task started: HMMsearch
[2024-01-24 12:37:02,970] [INFO] Running command: hmmsearch --tblout GCF_016765635.1_ASM1676563v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg970ce83c-61c9-4d95-a548-5b3c9fe9e7b2/dqc_reference/reference_markers.hmm GCF_016765635.1_ASM1676563v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:37:03,223] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:37:03,224] [INFO] Found 6/6 markers.
[2024-01-24 12:37:03,263] [INFO] Query marker FASTA was written to GCF_016765635.1_ASM1676563v1_genomic.fna/markers.fasta
[2024-01-24 12:37:03,263] [INFO] Task started: Blastn
[2024-01-24 12:37:03,263] [INFO] Running command: blastn -query GCF_016765635.1_ASM1676563v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg970ce83c-61c9-4d95-a548-5b3c9fe9e7b2/dqc_reference/reference_markers.fasta -out GCF_016765635.1_ASM1676563v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:37:04,018] [INFO] Task succeeded: Blastn
[2024-01-24 12:37:04,021] [INFO] Selected 11 target genomes.
[2024-01-24 12:37:04,021] [INFO] Target genome list was writen to GCF_016765635.1_ASM1676563v1_genomic.fna/target_genomes.txt
[2024-01-24 12:37:04,045] [INFO] Task started: fastANI
[2024-01-24 12:37:04,045] [INFO] Running command: fastANI --query /var/lib/cwl/stge4611906-35f4-44ee-b0b8-3efb39a9017d/GCF_016765635.1_ASM1676563v1_genomic.fna.gz --refList GCF_016765635.1_ASM1676563v1_genomic.fna/target_genomes.txt --output GCF_016765635.1_ASM1676563v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:37:16,956] [INFO] Task succeeded: fastANI
[2024-01-24 12:37:16,956] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg970ce83c-61c9-4d95-a548-5b3c9fe9e7b2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:37:16,956] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg970ce83c-61c9-4d95-a548-5b3c9fe9e7b2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:37:16,966] [INFO] Found 11 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 12:37:16,966] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:37:16,967] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Shewanella schlegeliana	strain=JCM 11561	GCA_016765635.1	190308	190308	type	True	100.0	1607	1608	95	conclusive
Shewanella schlegeliana	strain=LMG 21406	GCA_023283605.1	190308	190308	type	True	99.985	1592	1608	95	conclusive
Shewanella schlegeliana	strain=JCM 11561	GCA_019654995.1	190308	190308	type	True	99.981	1572	1608	95	conclusive
Shewanella halifaxensis	strain=HAW-EB4	GCA_000019185.1	271098	271098	type	True	86.219	1307	1608	95	below_threshold
Shewanella pealeana	strain=ATCC 700345	GCA_000018285.1	70864	70864	type	True	84.0362	1229	1608	95	below_threshold
Shewanella sairae	strain=JCM 11563	GCA_019655015.1	190310	190310	type	True	82.0296	892	1608	95	below_threshold
Shewanella marinintestina	strain=LMG 21403	GCA_023283845.1	190305	190305	type	True	81.9956	959	1608	95	below_threshold
Shewanella sairae	strain=LMG 21408	GCA_023283535.1	190310	190310	type	True	81.9207	885	1608	95	below_threshold
Shewanella fidelis	strain=ATCC BAA-318	GCA_000518605.1	173509	173509	type	True	81.5449	841	1608	95	below_threshold
Shewanella abyssi	strain=DSM 17171	GCA_023284025.1	311789	311789	type	True	79.5937	632	1608	95	below_threshold
Shewanella surugensis	strain=DSM 17177	GCA_023284005.1	212020	212020	type	True	78.4502	191	1608	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:37:16,968] [INFO] DFAST Taxonomy check result was written to GCF_016765635.1_ASM1676563v1_genomic.fna/tc_result.tsv
[2024-01-24 12:37:16,969] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:37:16,969] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:37:16,969] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg970ce83c-61c9-4d95-a548-5b3c9fe9e7b2/dqc_reference/checkm_data
[2024-01-24 12:37:16,970] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:37:17,013] [INFO] Task started: CheckM
[2024-01-24 12:37:17,014] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_016765635.1_ASM1676563v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_016765635.1_ASM1676563v1_genomic.fna/checkm_input GCF_016765635.1_ASM1676563v1_genomic.fna/checkm_result
[2024-01-24 12:37:51,329] [INFO] Task succeeded: CheckM
[2024-01-24 12:37:51,330] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:37:51,349] [INFO] ===== Completeness check finished =====
[2024-01-24 12:37:51,349] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:37:51,349] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_016765635.1_ASM1676563v1_genomic.fna/markers.fasta)
[2024-01-24 12:37:51,349] [INFO] Task started: Blastn
[2024-01-24 12:37:51,349] [INFO] Running command: blastn -query GCF_016765635.1_ASM1676563v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg970ce83c-61c9-4d95-a548-5b3c9fe9e7b2/dqc_reference/reference_markers_gtdb.fasta -out GCF_016765635.1_ASM1676563v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:37:52,481] [INFO] Task succeeded: Blastn
[2024-01-24 12:37:52,484] [INFO] Selected 9 target genomes.
[2024-01-24 12:37:52,484] [INFO] Target genome list was writen to GCF_016765635.1_ASM1676563v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:37:52,499] [INFO] Task started: fastANI
[2024-01-24 12:37:52,499] [INFO] Running command: fastANI --query /var/lib/cwl/stge4611906-35f4-44ee-b0b8-3efb39a9017d/GCF_016765635.1_ASM1676563v1_genomic.fna.gz --refList GCF_016765635.1_ASM1676563v1_genomic.fna/target_genomes_gtdb.txt --output GCF_016765635.1_ASM1676563v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:38:03,065] [INFO] Task succeeded: fastANI
[2024-01-24 12:38:03,074] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:38:03,074] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016765635.1	s__Shewanella schlegeliana	100.0	1607	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_000019185.1	s__Shewanella halifaxensis	86.2116	1309	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000018285.1	s__Shewanella pealeana	84.0484	1227	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000518605.1	s__Shewanella fidelis	81.5738	836	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella	95.0	97.11	97.11	0.90	0.90	2	-
GCF_000014885.1	s__Shewanella piezotolerans	80.1063	651	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016757755.1	s__Shewanella sp016757755	79.7732	665	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002836205.1	s__Shewanella sp002836205	79.6646	626	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella	95.0	99.46	99.13	0.96	0.92	4	-
GCF_000016065.1	s__Shewanella loihica	78.5352	301	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Shewanella	95.0	95.22	95.22	0.91	0.91	2	-
GCF_004358445.1	s__Parashewanella tropica	78.1563	141	1608	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Shewanellaceae;g__Parashewanella	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:38:03,075] [INFO] GTDB search result was written to GCF_016765635.1_ASM1676563v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:38:03,076] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:38:03,079] [INFO] DFAST_QC result json was written to GCF_016765635.1_ASM1676563v1_genomic.fna/dqc_result.json
[2024-01-24 12:38:03,079] [INFO] DFAST_QC completed!
[2024-01-24 12:38:03,079] [INFO] Total running time: 0h1m13s