[2024-01-25 20:13:05,801] [INFO] DFAST_QC pipeline started.
[2024-01-25 20:13:05,802] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 20:13:05,803] [INFO] DQC Reference Directory: /var/lib/cwl/stgdcdd507c-7993-4aeb-95c0-b60c574d1ecd/dqc_reference
[2024-01-25 20:13:06,972] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 20:13:06,973] [INFO] Task started: Prodigal
[2024-01-25 20:13:06,973] [INFO] Running command: gunzip -c /var/lib/cwl/stg3e62a9e3-7eec-4d04-8db2-be2778d8e877/GCF_016863535.1_ASM1686353v1_genomic.fna.gz | prodigal -d GCF_016863535.1_ASM1686353v1_genomic.fna/cds.fna -a GCF_016863535.1_ASM1686353v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 20:13:23,040] [INFO] Task succeeded: Prodigal
[2024-01-25 20:13:23,040] [INFO] Task started: HMMsearch
[2024-01-25 20:13:23,040] [INFO] Running command: hmmsearch --tblout GCF_016863535.1_ASM1686353v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgdcdd507c-7993-4aeb-95c0-b60c574d1ecd/dqc_reference/reference_markers.hmm GCF_016863535.1_ASM1686353v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 20:13:23,331] [INFO] Task succeeded: HMMsearch
[2024-01-25 20:13:23,332] [INFO] Found 6/6 markers.
[2024-01-25 20:13:23,385] [INFO] Query marker FASTA was written to GCF_016863535.1_ASM1686353v1_genomic.fna/markers.fasta
[2024-01-25 20:13:23,386] [INFO] Task started: Blastn
[2024-01-25 20:13:23,386] [INFO] Running command: blastn -query GCF_016863535.1_ASM1686353v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgdcdd507c-7993-4aeb-95c0-b60c574d1ecd/dqc_reference/reference_markers.fasta -out GCF_016863535.1_ASM1686353v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:13:24,482] [INFO] Task succeeded: Blastn
[2024-01-25 20:13:24,485] [INFO] Selected 20 target genomes.
[2024-01-25 20:13:24,485] [INFO] Target genome list was writen to GCF_016863535.1_ASM1686353v1_genomic.fna/target_genomes.txt
[2024-01-25 20:13:24,496] [INFO] Task started: fastANI
[2024-01-25 20:13:24,496] [INFO] Running command: fastANI --query /var/lib/cwl/stg3e62a9e3-7eec-4d04-8db2-be2778d8e877/GCF_016863535.1_ASM1686353v1_genomic.fna.gz --refList GCF_016863535.1_ASM1686353v1_genomic.fna/target_genomes.txt --output GCF_016863535.1_ASM1686353v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 20:13:55,703] [INFO] Task succeeded: fastANI
[2024-01-25 20:13:55,703] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgdcdd507c-7993-4aeb-95c0-b60c574d1ecd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 20:13:55,704] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgdcdd507c-7993-4aeb-95c0-b60c574d1ecd/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 20:13:55,717] [INFO] Found 20 fastANI hits (0 hits with ANI > threshold)
[2024-01-25 20:13:55,717] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-25 20:13:55,718] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Micromonospora phaseoli	strain=NBRC 110907	GCA_016863675.1	1144548	1144548	type	True	87.6791	1511	2003	95	below_threshold
Micromonospora phaseoli	strain=CGMCC 4.7038	GCA_900109115.1	1144548	1144548	type	True	87.6629	1532	2003	95	below_threshold
Micromonospora tarapacensis	strain=Llam7	GCA_019697375.1	2835305	2835305	type	True	87.5266	1419	2003	95	below_threshold
Micromonospora craterilacus	strain=NA12	GCA_003236315.1	1655439	1655439	type	True	86.8517	1263	2003	95	below_threshold
Micromonospora zingiberis	strain=PLAI 1-1	GCA_004331455.1	2053011	2053011	type	True	86.3161	1368	2003	95	below_threshold
Micromonospora radicis	strain=AZ1-13	GCA_003583405.1	1894971	1894971	type	True	86.1829	1335	2003	95	below_threshold
Micromonospora fiedleri	strain=MG-37	GCA_016774385.1	1157498	1157498	type	True	85.9261	1456	2003	95	below_threshold
Micromonospora maris	strain=AB-18-032	GCA_000204155.1	1003110	1003110	type	True	85.8936	1462	2003	95	below_threshold
Micromonospora maris	strain=NRRL B-24793	GCA_001507315.1	1003110	1003110	type	True	85.8805	1447	2003	95	below_threshold
Micromonospora craniellae	strain=LHW63014	GCA_014764405.1	2294034	2294034	type	True	85.8465	1293	2003	95	below_threshold
Micromonospora craniellae	strain=LHW63014	GCA_003419945.1	2294034	2294034	type	True	85.8327	1291	2003	95	below_threshold
Micromonospora gifhornensis	strain=NBRC 16317	GCA_016863515.1	84594	84594	type	True	85.7181	1423	2003	95	below_threshold
Micromonospora phytophila	strain=DSM 105363	GCA_023656545.1	709888	709888	type	True	84.1212	944	2003	95	below_threshold
Micromonospora endolithica	strain=JCM 12677	GCA_003626595.1	230091	230091	type	True	83.6605	1288	2003	95	below_threshold
Micromonospora endolithica	strain=DSM 44398	GCA_007994185.1	230091	230091	type	True	83.6545	1272	2003	95	below_threshold
Micromonospora coxensis	strain=DSM 45161	GCA_900090295.1	356852	356852	type	True	83.5208	1271	2003	95	below_threshold
Micromonospora acroterricola	strain=5R2A7	GCA_003172955.1	2202421	2202421	type	True	83.1993	1109	2003	95	below_threshold
Micromonospora carbonacea	strain=aurantiaca	GCA_013389765.1	47853	47853	type	True	83.1414	1249	2003	95	below_threshold
Micromonospora humida	strain=MMS20-R1-14	GCA_016901255.1	2809018	2809018	type	True	83.0971	1250	2003	95	below_threshold
Micromonospora ferruginea	strain=28ISP2-46	GCA_013694245.1	2749844	2749844	type	True	82.9018	1240	2003	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 20:13:55,719] [INFO] DFAST Taxonomy check result was written to GCF_016863535.1_ASM1686353v1_genomic.fna/tc_result.tsv
[2024-01-25 20:13:55,720] [INFO] ===== Taxonomy check completed =====
[2024-01-25 20:13:55,720] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 20:13:55,720] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgdcdd507c-7993-4aeb-95c0-b60c574d1ecd/dqc_reference/checkm_data
[2024-01-25 20:13:55,721] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 20:13:55,777] [INFO] Task started: CheckM
[2024-01-25 20:13:55,777] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_016863535.1_ASM1686353v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_016863535.1_ASM1686353v1_genomic.fna/checkm_input GCF_016863535.1_ASM1686353v1_genomic.fna/checkm_result
[2024-01-25 20:14:57,476] [INFO] Task succeeded: CheckM
[2024-01-25 20:14:57,476] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 20:14:57,491] [INFO] ===== Completeness check finished =====
[2024-01-25 20:14:57,491] [INFO] ===== Start GTDB Search =====
[2024-01-25 20:14:57,492] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_016863535.1_ASM1686353v1_genomic.fna/markers.fasta)
[2024-01-25 20:14:57,492] [INFO] Task started: Blastn
[2024-01-25 20:14:57,492] [INFO] Running command: blastn -query GCF_016863535.1_ASM1686353v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgdcdd507c-7993-4aeb-95c0-b60c574d1ecd/dqc_reference/reference_markers_gtdb.fasta -out GCF_016863535.1_ASM1686353v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:14:59,121] [INFO] Task succeeded: Blastn
[2024-01-25 20:14:59,124] [INFO] Selected 15 target genomes.
[2024-01-25 20:14:59,124] [INFO] Target genome list was writen to GCF_016863535.1_ASM1686353v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 20:14:59,150] [INFO] Task started: fastANI
[2024-01-25 20:14:59,150] [INFO] Running command: fastANI --query /var/lib/cwl/stg3e62a9e3-7eec-4d04-8db2-be2778d8e877/GCF_016863535.1_ASM1686353v1_genomic.fna.gz --refList GCF_016863535.1_ASM1686353v1_genomic.fna/target_genomes_gtdb.txt --output GCF_016863535.1_ASM1686353v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 20:15:23,765] [INFO] Task succeeded: fastANI
[2024-01-25 20:15:23,775] [INFO] Found 15 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 20:15:23,775] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016863535.1	s__Micromonospora lutea	100.0	2000	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_009712475.1	s__Micromonospora sp009712475	91.0871	1590	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016863495.1	s__Micromonospora andamanensis	90.7549	1607	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	96.71	95.67	0.86	0.84	3	-
GCF_900112325.1	s__Micromonospora sediminimaris	90.3839	1609	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	98.06	96.13	0.95	0.90	3	-
GCF_010560025.1	s__Micromonospora sp010560025	90.1338	1561	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	99.75	99.70	1.00	1.00	3	-
GCF_018070465.1	s__Micromonospora sp018070465	90.1185	1510	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900109115.1	s__Micromonospora phaseoli	87.6559	1533	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	100.00	100.00	1.00	1.00	3	-
GCF_003236315.1	s__Micromonospora craterilacus	86.8035	1269	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004331455.1	s__Micromonospora zingiberis	86.2916	1371	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003583405.1	s__Micromonospora radicis	86.1982	1333	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016863555.1	s__Micromonospora qiuiae	86.0487	1346	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013364215.1	s__Micromonospora sp004307965	85.9489	1440	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	99.04	99.04	0.94	0.94	2	-
GCF_014764405.1	s__Micromonospora craniellae	85.8482	1293	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	99.95	99.95	0.98	0.98	2	-
GCF_016863515.1	s__Micromonospora gifhornensis	85.7223	1423	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	96.77	95.90	0.88	0.87	6	-
GCF_003725545.1	s__Micromonospora sp003725545	83.2626	1123	2003	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Micromonosporaceae;g__Micromonospora	95.0	99.98	99.95	0.98	0.97	4	-
--------------------------------------------------------------------------------
[2024-01-25 20:15:23,776] [INFO] GTDB search result was written to GCF_016863535.1_ASM1686353v1_genomic.fna/result_gtdb.tsv
[2024-01-25 20:15:23,776] [INFO] ===== GTDB Search completed =====
[2024-01-25 20:15:23,781] [INFO] DFAST_QC result json was written to GCF_016863535.1_ASM1686353v1_genomic.fna/dqc_result.json
[2024-01-25 20:15:23,781] [INFO] DFAST_QC completed!
[2024-01-25 20:15:23,781] [INFO] Total running time: 0h2m18s