[2024-01-24 14:54:56,332] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:54:56,335] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:54:56,336] [INFO] DQC Reference Directory: /var/lib/cwl/stg0560a7fa-d975-4b70-97f3-cdd575973d13/dqc_reference
[2024-01-24 14:54:57,749] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:54:57,750] [INFO] Task started: Prodigal
[2024-01-24 14:54:57,751] [INFO] Running command: gunzip -c /var/lib/cwl/stgec78976a-dbd9-4002-814d-52b6d56eb92e/GCF_016894445.1_ASM1689444v1_genomic.fna.gz | prodigal -d GCF_016894445.1_ASM1689444v1_genomic.fna/cds.fna -a GCF_016894445.1_ASM1689444v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:55:31,574] [INFO] Task succeeded: Prodigal
[2024-01-24 14:55:31,575] [INFO] Task started: HMMsearch
[2024-01-24 14:55:31,575] [INFO] Running command: hmmsearch --tblout GCF_016894445.1_ASM1689444v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg0560a7fa-d975-4b70-97f3-cdd575973d13/dqc_reference/reference_markers.hmm GCF_016894445.1_ASM1689444v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:55:31,976] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:55:31,978] [INFO] Found 6/6 markers.
[2024-01-24 14:55:32,032] [INFO] Query marker FASTA was written to GCF_016894445.1_ASM1689444v1_genomic.fna/markers.fasta
[2024-01-24 14:55:32,033] [INFO] Task started: Blastn
[2024-01-24 14:55:32,033] [INFO] Running command: blastn -query GCF_016894445.1_ASM1689444v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg0560a7fa-d975-4b70-97f3-cdd575973d13/dqc_reference/reference_markers.fasta -out GCF_016894445.1_ASM1689444v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:55:32,670] [INFO] Task succeeded: Blastn
[2024-01-24 14:55:32,673] [INFO] Selected 18 target genomes.
[2024-01-24 14:55:32,674] [INFO] Target genome list was writen to GCF_016894445.1_ASM1689444v1_genomic.fna/target_genomes.txt
[2024-01-24 14:55:32,681] [INFO] Task started: fastANI
[2024-01-24 14:55:32,681] [INFO] Running command: fastANI --query /var/lib/cwl/stgec78976a-dbd9-4002-814d-52b6d56eb92e/GCF_016894445.1_ASM1689444v1_genomic.fna.gz --refList GCF_016894445.1_ASM1689444v1_genomic.fna/target_genomes.txt --output GCF_016894445.1_ASM1689444v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:55:53,558] [INFO] Task succeeded: fastANI
[2024-01-24 14:55:53,559] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg0560a7fa-d975-4b70-97f3-cdd575973d13/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:55:53,559] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg0560a7fa-d975-4b70-97f3-cdd575973d13/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:55:53,573] [INFO] Found 13 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 14:55:53,573] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:55:53,574] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Dyadobacter sandarakinus	strain=Q3-56	GCA_016894445.1	2747268	2747268	type	True	100.0	2022	2023	95	conclusive
Dyadobacter luticola	strain=T17	GCA_005860805.1	1979387	1979387	type	True	78.3123	550	2023	95	below_threshold
Dyadobacter crusticola	strain=DSM 16708	GCA_000701505.1	292407	292407	type	True	77.9576	485	2023	95	below_threshold
Dyadobacter beijingensis	strain=DSM 21582	GCA_000382205.1	365489	365489	type	True	77.8578	530	2023	95	below_threshold
Dyadobacter alkalitolerans	strain=DSM 23607	GCA_000428845.1	492736	492736	type	True	77.8422	434	2023	95	below_threshold
Dyadobacter beijingensis	strain=CGMCC 1.6375	GCA_014645155.1	365489	365489	type	True	77.8415	522	2023	95	below_threshold
Dyadobacter psychrophilus	strain=DSM 22270	GCA_900167945.1	651661	651661	type	True	77.8333	415	2023	95	below_threshold
Dyadobacter bucti	strain=QTA69	GCA_005869225.1	2572203	2572203	type	True	77.7372	423	2023	95	below_threshold
Dyadobacter flavalbus	strain=NS28	GCA_006149045.1	2579942	2579942	type	True	77.7275	497	2023	95	below_threshold
Dyadobacter jiangsuensis	strain=DSM 29057	GCA_003014695.1	1591085	1591085	type	True	77.6608	484	2023	95	below_threshold
Dyadobacter endophyticus	strain=CGMCC 1.15288	GCA_014641595.1	1749036	1749036	type	True	77.6603	463	2023	95	below_threshold
Dyadobacter sediminis	strain=Z12	GCA_005860765.1	1493691	1493691	type	True	77.5933	525	2023	95	below_threshold
Dyadobacter sediminis	strain=CGMCC 1.12895	GCA_014640015.1	1493691	1493691	type	True	77.5827	532	2023	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:55:53,575] [INFO] DFAST Taxonomy check result was written to GCF_016894445.1_ASM1689444v1_genomic.fna/tc_result.tsv
[2024-01-24 14:55:53,576] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:55:53,576] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:55:53,576] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg0560a7fa-d975-4b70-97f3-cdd575973d13/dqc_reference/checkm_data
[2024-01-24 14:55:53,577] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:55:53,635] [INFO] Task started: CheckM
[2024-01-24 14:55:53,635] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_016894445.1_ASM1689444v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_016894445.1_ASM1689444v1_genomic.fna/checkm_input GCF_016894445.1_ASM1689444v1_genomic.fna/checkm_result
[2024-01-24 14:57:22,076] [INFO] Task succeeded: CheckM
[2024-01-24 14:57:22,078] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:57:22,100] [INFO] ===== Completeness check finished =====
[2024-01-24 14:57:22,101] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:57:22,101] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_016894445.1_ASM1689444v1_genomic.fna/markers.fasta)
[2024-01-24 14:57:22,101] [INFO] Task started: Blastn
[2024-01-24 14:57:22,102] [INFO] Running command: blastn -query GCF_016894445.1_ASM1689444v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg0560a7fa-d975-4b70-97f3-cdd575973d13/dqc_reference/reference_markers_gtdb.fasta -out GCF_016894445.1_ASM1689444v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:57:22,927] [INFO] Task succeeded: Blastn
[2024-01-24 14:57:22,930] [INFO] Selected 21 target genomes.
[2024-01-24 14:57:22,930] [INFO] Target genome list was writen to GCF_016894445.1_ASM1689444v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:57:22,953] [INFO] Task started: fastANI
[2024-01-24 14:57:22,953] [INFO] Running command: fastANI --query /var/lib/cwl/stgec78976a-dbd9-4002-814d-52b6d56eb92e/GCF_016894445.1_ASM1689444v1_genomic.fna.gz --refList GCF_016894445.1_ASM1689444v1_genomic.fna/target_genomes_gtdb.txt --output GCF_016894445.1_ASM1689444v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:57:50,089] [INFO] Task succeeded: fastANI
[2024-01-24 14:57:50,109] [INFO] Found 17 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:57:50,110] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016894445.1	s__Dyadobacter sp016894445	100.0	2022	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_005860805.1	s__Dyadobacter luticola	78.3157	548	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001424405.1	s__Dyadobacter sp001424405	78.1001	498	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_008369915.1	s__Dyadobacter sp008369915	77.9984	447	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000701505.1	s__Dyadobacter crusticola	77.9783	479	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000382205.1	s__Dyadobacter beijingensis	77.8636	528	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	100.00	100.00	1.00	1.00	2	-
GCF_000428845.1	s__Dyadobacter alkalitolerans	77.8478	434	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900101885.1	s__Dyadobacter soli	77.8393	541	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900167945.1	s__Dyadobacter psychrophilus	77.8393	413	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_907165045.1	s__Dyadobacter sp907165045	77.7951	460	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900109045.1	s__Dyadobacter sp900109045	77.7845	470	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_005869225.1	s__Dyadobacter bucti	77.7315	422	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_006149045.1	s__Dyadobacter flavalbus	77.7297	496	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003014695.1	s__Dyadobacter jiangsuensis	77.6686	480	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014641595.1	s__Dyadobacter endophyticus	77.6603	463	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_005860765.1	s__Dyadobacter sediminis	77.5994	524	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	100.00	100.00	1.00	1.00	2	-
GCA_001898145.1	s__Dyadobacter sp001898145	77.5793	496	2023	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Dyadobacter	95.0	99.32	99.32	0.98	0.98	2	-
--------------------------------------------------------------------------------
[2024-01-24 14:57:50,112] [INFO] GTDB search result was written to GCF_016894445.1_ASM1689444v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:57:50,113] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:57:50,118] [INFO] DFAST_QC result json was written to GCF_016894445.1_ASM1689444v1_genomic.fna/dqc_result.json
[2024-01-24 14:57:50,118] [INFO] DFAST_QC completed!
[2024-01-24 14:57:50,118] [INFO] Total running time: 0h2m54s