[2024-01-24 11:05:26,660] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:05:26,664] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:05:26,665] [INFO] DQC Reference Directory: /var/lib/cwl/stgc8963a52-998c-4f09-8dcf-4252639ffb64/dqc_reference
[2024-01-24 11:05:28,024] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:05:28,025] [INFO] Task started: Prodigal
[2024-01-24 11:05:28,026] [INFO] Running command: gunzip -c /var/lib/cwl/stge39499b5-9c99-4f46-b23c-9ce48205c3dc/GCF_017353495.1_ASM1735349v1_genomic.fna.gz | prodigal -d GCF_017353495.1_ASM1735349v1_genomic.fna/cds.fna -a GCF_017353495.1_ASM1735349v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:05:40,312] [INFO] Task succeeded: Prodigal
[2024-01-24 11:05:40,313] [INFO] Task started: HMMsearch
[2024-01-24 11:05:40,313] [INFO] Running command: hmmsearch --tblout GCF_017353495.1_ASM1735349v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgc8963a52-998c-4f09-8dcf-4252639ffb64/dqc_reference/reference_markers.hmm GCF_017353495.1_ASM1735349v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:05:40,602] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:05:40,604] [INFO] Found 6/6 markers.
[2024-01-24 11:05:40,662] [INFO] Query marker FASTA was written to GCF_017353495.1_ASM1735349v1_genomic.fna/markers.fasta
[2024-01-24 11:05:40,662] [INFO] Task started: Blastn
[2024-01-24 11:05:40,663] [INFO] Running command: blastn -query GCF_017353495.1_ASM1735349v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc8963a52-998c-4f09-8dcf-4252639ffb64/dqc_reference/reference_markers.fasta -out GCF_017353495.1_ASM1735349v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:05:41,740] [INFO] Task succeeded: Blastn
[2024-01-24 11:05:41,743] [INFO] Selected 7 target genomes.
[2024-01-24 11:05:41,743] [INFO] Target genome list was writen to GCF_017353495.1_ASM1735349v1_genomic.fna/target_genomes.txt
[2024-01-24 11:05:41,747] [INFO] Task started: fastANI
[2024-01-24 11:05:41,747] [INFO] Running command: fastANI --query /var/lib/cwl/stge39499b5-9c99-4f46-b23c-9ce48205c3dc/GCF_017353495.1_ASM1735349v1_genomic.fna.gz --refList GCF_017353495.1_ASM1735349v1_genomic.fna/target_genomes.txt --output GCF_017353495.1_ASM1735349v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:05:48,121] [INFO] Task succeeded: fastANI
[2024-01-24 11:05:48,122] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgc8963a52-998c-4f09-8dcf-4252639ffb64/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:05:48,122] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgc8963a52-998c-4f09-8dcf-4252639ffb64/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:05:48,130] [INFO] Found 7 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 11:05:48,130] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:05:48,130] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Arthrobacter sunyaminii	strain=zg-ZUI122	GCA_017353495.1	2816859	2816859	type	True	100.0	1305	1306	95	conclusive
Arthrobacter sunyaminii	strain=zg-ZUI122	GCA_018866305.1	2816859	2816859	type	True	99.9989	1290	1306	95	conclusive
Arthrobacter jiangjiafuii	strain=zg-ZUI227	GCA_018622995.1	2817475	2817475	type	True	83.313	776	1306	95	below_threshold
Arthrobacter jiangjiafuii	strain=zg-ZUI227	GCA_017898065.1	2817475	2817475	type	True	83.2803	775	1306	95	below_threshold
Arthrobacter gandavensis	strain=JCM 13316	GCA_009192735.1	169960	169960	type	True	83.2042	806	1306	95	below_threshold
Arthrobacter citreus	strain=DSM 20133	GCA_009192745.1	1670	1670	type	True	80.4975	554	1306	95	below_threshold
Arthrobacter gallicola	strain=Sa2CUA1	GCA_014836775.1	2762225	2762225	type	True	80.2393	544	1306	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:05:48,135] [INFO] DFAST Taxonomy check result was written to GCF_017353495.1_ASM1735349v1_genomic.fna/tc_result.tsv
[2024-01-24 11:05:48,136] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:05:48,136] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:05:48,136] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgc8963a52-998c-4f09-8dcf-4252639ffb64/dqc_reference/checkm_data
[2024-01-24 11:05:48,137] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:05:48,190] [INFO] Task started: CheckM
[2024-01-24 11:05:48,190] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_017353495.1_ASM1735349v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_017353495.1_ASM1735349v1_genomic.fna/checkm_input GCF_017353495.1_ASM1735349v1_genomic.fna/checkm_result
[2024-01-24 11:06:26,716] [INFO] Task succeeded: CheckM
[2024-01-24 11:06:26,717] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:06:26,736] [INFO] ===== Completeness check finished =====
[2024-01-24 11:06:26,736] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:06:26,736] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_017353495.1_ASM1735349v1_genomic.fna/markers.fasta)
[2024-01-24 11:06:26,737] [INFO] Task started: Blastn
[2024-01-24 11:06:26,737] [INFO] Running command: blastn -query GCF_017353495.1_ASM1735349v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgc8963a52-998c-4f09-8dcf-4252639ffb64/dqc_reference/reference_markers_gtdb.fasta -out GCF_017353495.1_ASM1735349v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:06:28,297] [INFO] Task succeeded: Blastn
[2024-01-24 11:06:28,302] [INFO] Selected 9 target genomes.
[2024-01-24 11:06:28,303] [INFO] Target genome list was writen to GCF_017353495.1_ASM1735349v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:06:28,311] [INFO] Task started: fastANI
[2024-01-24 11:06:28,312] [INFO] Running command: fastANI --query /var/lib/cwl/stge39499b5-9c99-4f46-b23c-9ce48205c3dc/GCF_017353495.1_ASM1735349v1_genomic.fna.gz --refList GCF_017353495.1_ASM1735349v1_genomic.fna/target_genomes_gtdb.txt --output GCF_017353495.1_ASM1735349v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:06:35,912] [INFO] Task succeeded: fastANI
[2024-01-24 11:06:35,926] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:06:35,926] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_018866305.1	s__Arthrobacter_B sp002929435	99.9989	1290	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	98.36	96.67	0.94	0.92	8	conclusive
GCF_018622995.1	s__Arthrobacter_B sp018622995	83.3184	776	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	99.13	98.27	0.96	0.92	3	-
GCF_009192735.1	s__Arthrobacter_B gandavensis	83.2247	803	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009192775.1	s__Arthrobacter_B yangruifuii	81.2129	619	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	97.52	97.52	0.93	0.93	2	-
GCF_009192745.1	s__Arthrobacter_B citreus	80.5029	553	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014836875.1	s__Arthrobacter_B sp014836875	80.4524	541	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014836775.1	s__Arthrobacter_B sp014836775	80.229	545	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001307035.1	s__Arthrobacter_B sp001307035	80.1788	549	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	98.39	98.39	0.92	0.92	2	-
GCA_900016675.1	s__Arthrobacter_B saudimassiliensis	80.032	577	1306	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Micrococcaceae;g__Arthrobacter_B	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:06:35,928] [INFO] GTDB search result was written to GCF_017353495.1_ASM1735349v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:06:35,928] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:06:35,931] [INFO] DFAST_QC result json was written to GCF_017353495.1_ASM1735349v1_genomic.fna/dqc_result.json
[2024-01-24 11:06:35,931] [INFO] DFAST_QC completed!
[2024-01-24 11:06:35,931] [INFO] Total running time: 0h1m9s