[2024-01-25 19:01:35,551] [INFO] DFAST_QC pipeline started.
[2024-01-25 19:01:35,553] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 19:01:35,553] [INFO] DQC Reference Directory: /var/lib/cwl/stg2778e046-1907-43b7-984f-d5cd1900e1f9/dqc_reference
[2024-01-25 19:01:36,676] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 19:01:36,677] [INFO] Task started: Prodigal
[2024-01-25 19:01:36,677] [INFO] Running command: gunzip -c /var/lib/cwl/stg027b030d-37bf-4473-b4cf-09f2d9a4a304/GCF_017581985.1_ASM1758198v1_genomic.fna.gz | prodigal -d GCF_017581985.1_ASM1758198v1_genomic.fna/cds.fna -a GCF_017581985.1_ASM1758198v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 19:01:46,511] [INFO] Task succeeded: Prodigal
[2024-01-25 19:01:46,512] [INFO] Task started: HMMsearch
[2024-01-25 19:01:46,512] [INFO] Running command: hmmsearch --tblout GCF_017581985.1_ASM1758198v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2778e046-1907-43b7-984f-d5cd1900e1f9/dqc_reference/reference_markers.hmm GCF_017581985.1_ASM1758198v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 19:01:46,724] [INFO] Task succeeded: HMMsearch
[2024-01-25 19:01:46,725] [INFO] Found 6/6 markers.
[2024-01-25 19:01:46,757] [INFO] Query marker FASTA was written to GCF_017581985.1_ASM1758198v1_genomic.fna/markers.fasta
[2024-01-25 19:01:46,757] [INFO] Task started: Blastn
[2024-01-25 19:01:46,757] [INFO] Running command: blastn -query GCF_017581985.1_ASM1758198v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2778e046-1907-43b7-984f-d5cd1900e1f9/dqc_reference/reference_markers.fasta -out GCF_017581985.1_ASM1758198v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:01:47,982] [INFO] Task succeeded: Blastn
[2024-01-25 19:01:47,985] [INFO] Selected 15 target genomes.
[2024-01-25 19:01:47,986] [INFO] Target genome list was writen to GCF_017581985.1_ASM1758198v1_genomic.fna/target_genomes.txt
[2024-01-25 19:01:48,000] [INFO] Task started: fastANI
[2024-01-25 19:01:48,000] [INFO] Running command: fastANI --query /var/lib/cwl/stg027b030d-37bf-4473-b4cf-09f2d9a4a304/GCF_017581985.1_ASM1758198v1_genomic.fna.gz --refList GCF_017581985.1_ASM1758198v1_genomic.fna/target_genomes.txt --output GCF_017581985.1_ASM1758198v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 19:02:02,764] [INFO] Task succeeded: fastANI
[2024-01-25 19:02:02,764] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2778e046-1907-43b7-984f-d5cd1900e1f9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 19:02:02,764] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2778e046-1907-43b7-984f-d5cd1900e1f9/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 19:02:02,774] [INFO] Found 15 fastANI hits (0 hits with ANI > threshold)
[2024-01-25 19:02:02,774] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-25 19:02:02,774] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Cellulomonas avistercoris	strain=Sa3CUA2	GCA_014836445.1	2762242	2762242	type	True	93.6163	1125	1285	95	below_threshold
Cellulomonas flavigena	strain=DSM 20109	GCA_000092865.1	1711	1711	type	True	85.3942	979	1285	95	below_threshold
Cellulomonas iranensis	strain=NBRC 101100	GCA_001552375.1	76862	76862	type	True	84.5078	845	1285	95	below_threshold
Cellulomonas shaoxiangyii	strain=Z28	GCA_004798685.1	2566013	2566013	type	True	82.9257	865	1285	95	below_threshold
Cellulomonas telluris	strain=CPCC 204705	GCA_004886325.1	2306636	2306636	type	True	82.5013	845	1285	95	below_threshold
Cellulomonas oligotrophica	strain=DSM 24482	GCA_013409875.1	931536	931536	type	True	82.0375	828	1285	95	below_threshold
Cellulomonas palmilytica	strain=EW123	GCA_021590045.1	2608402	2608402	type	True	81.1632	724	1285	95	below_threshold
Cellulomonas fimi	strain=NCTC7547	GCA_900637695.1	1708	1708	type	True	81.0841	742	1285	95	below_threshold
Cellulomonas biazotea	strain=NBRC12680	GCA_004306155.1	1709	1709	type	True	81.0357	680	1285	95	below_threshold
Cellulomonas fimi	strain=ATCC 484	GCA_000212695.1	1708	1708	type	True	80.9601	768	1285	95	below_threshold
Cellulomonas composti	strain=NBRC 100758	GCA_007990245.1	266130	266130	type	True	80.4856	645	1285	95	below_threshold
Cellulomonas massiliensis	strain=JC225	GCA_000312005.1	1465811	1465811	type	True	80.4845	676	1285	95	below_threshold
Cellulomonas fulva	strain=DKR-3	GCA_018531375.1	2835530	2835530	type	True	80.4329	687	1285	95	below_threshold
Actinotalea solisilvae	strain=KACC 19191	GCA_016464425.1	2072922	2072922	type	True	79.2302	653	1285	95	below_threshold
Actinotalea subterranea	strain=HO-Ch2	GCA_008364845.1	2607497	2607497	type	True	79.1442	577	1285	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 19:02:02,776] [INFO] DFAST Taxonomy check result was written to GCF_017581985.1_ASM1758198v1_genomic.fna/tc_result.tsv
[2024-01-25 19:02:02,776] [INFO] ===== Taxonomy check completed =====
[2024-01-25 19:02:02,776] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 19:02:02,776] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2778e046-1907-43b7-984f-d5cd1900e1f9/dqc_reference/checkm_data
[2024-01-25 19:02:02,777] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 19:02:02,816] [INFO] Task started: CheckM
[2024-01-25 19:02:02,817] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_017581985.1_ASM1758198v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_017581985.1_ASM1758198v1_genomic.fna/checkm_input GCF_017581985.1_ASM1758198v1_genomic.fna/checkm_result
[2024-01-25 19:03:11,559] [INFO] Task succeeded: CheckM
[2024-01-25 19:03:11,560] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 19:03:11,576] [INFO] ===== Completeness check finished =====
[2024-01-25 19:03:11,576] [INFO] ===== Start GTDB Search =====
[2024-01-25 19:03:11,577] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_017581985.1_ASM1758198v1_genomic.fna/markers.fasta)
[2024-01-25 19:03:11,577] [INFO] Task started: Blastn
[2024-01-25 19:03:11,577] [INFO] Running command: blastn -query GCF_017581985.1_ASM1758198v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2778e046-1907-43b7-984f-d5cd1900e1f9/dqc_reference/reference_markers_gtdb.fasta -out GCF_017581985.1_ASM1758198v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:03:13,592] [INFO] Task succeeded: Blastn
[2024-01-25 19:03:13,599] [INFO] Selected 12 target genomes.
[2024-01-25 19:03:13,599] [INFO] Target genome list was writen to GCF_017581985.1_ASM1758198v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 19:03:13,614] [INFO] Task started: fastANI
[2024-01-25 19:03:13,614] [INFO] Running command: fastANI --query /var/lib/cwl/stg027b030d-37bf-4473-b4cf-09f2d9a4a304/GCF_017581985.1_ASM1758198v1_genomic.fna.gz --refList GCF_017581985.1_ASM1758198v1_genomic.fna/target_genomes_gtdb.txt --output GCF_017581985.1_ASM1758198v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 19:03:26,938] [INFO] Task succeeded: fastANI
[2024-01-25 19:03:26,947] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 19:03:26,947] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_018623035.1	s__Cellulomonas sp018623035	99.9977	1284	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	99.51	99.03	0.98	0.95	3	conclusive
GCF_014836445.1	s__Cellulomonas sp014836445	93.6248	1123	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015234515.1	s__Cellulomonas sp015234515	88.1578	1027	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016862775.1	s__Cellulomonas phragmiteti	86.114	947	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000092865.1	s__Cellulomonas flavigena	85.3826	979	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018388445.1	s__Cellulomonas sp018388445	85.356	975	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	99.55	99.30	0.98	0.97	4	-
GCF_900114185.1	s__Cellulomonas sp900114185	85.3265	976	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001552375.1	s__Cellulomonas iranensis	84.5057	846	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	98.21	97.89	0.91	0.88	6	-
GCF_001462455.1	s__Cellulomonas sp001462455	84.4914	816	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009720485.1	s__Cellulomonas sp009720485	82.5177	840	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004886325.1	s__Cellulomonas telluris	82.5035	844	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000212695.1	s__Cellulomonas fimi	81.0662	754	1285	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Cellulomonadaceae;g__Cellulomonas	95.0	100.00	99.99	1.00	1.00	3	-
--------------------------------------------------------------------------------
[2024-01-25 19:03:26,948] [INFO] GTDB search result was written to GCF_017581985.1_ASM1758198v1_genomic.fna/result_gtdb.tsv
[2024-01-25 19:03:26,949] [INFO] ===== GTDB Search completed =====
[2024-01-25 19:03:26,952] [INFO] DFAST_QC result json was written to GCF_017581985.1_ASM1758198v1_genomic.fna/dqc_result.json
[2024-01-25 19:03:26,952] [INFO] DFAST_QC completed!
[2024-01-25 19:03:26,952] [INFO] Total running time: 0h1m51s