[2024-01-24 13:22:55,694] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:22:55,696] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:22:55,696] [INFO] DQC Reference Directory: /var/lib/cwl/stg7eac07df-6250-445b-bb99-ac462991293d/dqc_reference
[2024-01-24 13:22:56,991] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:22:56,992] [INFO] Task started: Prodigal
[2024-01-24 13:22:56,992] [INFO] Running command: gunzip -c /var/lib/cwl/stgd560a7ee-10f9-4f27-aa55-ae150c2ec53d/GCF_017874835.1_ASM1787483v1_genomic.fna.gz | prodigal -d GCF_017874835.1_ASM1787483v1_genomic.fna/cds.fna -a GCF_017874835.1_ASM1787483v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:23:09,645] [INFO] Task succeeded: Prodigal
[2024-01-24 13:23:09,646] [INFO] Task started: HMMsearch
[2024-01-24 13:23:09,646] [INFO] Running command: hmmsearch --tblout GCF_017874835.1_ASM1787483v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg7eac07df-6250-445b-bb99-ac462991293d/dqc_reference/reference_markers.hmm GCF_017874835.1_ASM1787483v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:23:09,957] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:23:09,959] [INFO] Found 6/6 markers.
[2024-01-24 13:23:10,004] [INFO] Query marker FASTA was written to GCF_017874835.1_ASM1787483v1_genomic.fna/markers.fasta
[2024-01-24 13:23:10,004] [INFO] Task started: Blastn
[2024-01-24 13:23:10,004] [INFO] Running command: blastn -query GCF_017874835.1_ASM1787483v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7eac07df-6250-445b-bb99-ac462991293d/dqc_reference/reference_markers.fasta -out GCF_017874835.1_ASM1787483v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:23:10,679] [INFO] Task succeeded: Blastn
[2024-01-24 13:23:10,682] [INFO] Selected 14 target genomes.
[2024-01-24 13:23:10,683] [INFO] Target genome list was writen to GCF_017874835.1_ASM1787483v1_genomic.fna/target_genomes.txt
[2024-01-24 13:23:10,697] [INFO] Task started: fastANI
[2024-01-24 13:23:10,697] [INFO] Running command: fastANI --query /var/lib/cwl/stgd560a7ee-10f9-4f27-aa55-ae150c2ec53d/GCF_017874835.1_ASM1787483v1_genomic.fna.gz --refList GCF_017874835.1_ASM1787483v1_genomic.fna/target_genomes.txt --output GCF_017874835.1_ASM1787483v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:23:26,611] [INFO] Task succeeded: fastANI
[2024-01-24 13:23:26,612] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg7eac07df-6250-445b-bb99-ac462991293d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:23:26,613] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg7eac07df-6250-445b-bb99-ac462991293d/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:23:26,626] [INFO] Found 14 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 13:23:26,627] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:23:26,627] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Cohnella lubricantis	strain=DSM 103658	GCA_017874835.1	2163172	2163172	type	True	100.0	1462	1466	95	conclusive
Cohnella lubricantis	strain=DSM 103658	GCA_014212015.1	2163172	2163172	type	True	99.9683	1403	1466	95	conclusive
Cohnella xylanilytica	strain=DSM 25239	GCA_014212175.1	557555	557555	type	True	81.1877	753	1466	95	below_threshold
Cohnella thermotolerans	strain=DSM 17683	GCA_000429825.1	329858	329858	type	True	80.7868	689	1466	95	below_threshold
Cohnella zeiphila	strain=CBP-2801	GCA_014212055.1	2761120	2761120	type	True	79.6745	596	1466	95	below_threshold
Cohnella fermenti	strain=CC-MHH1044	GCA_004801405.1	2565925	2565925	type	True	79.3422	553	1466	95	below_threshold
Cohnella nanjingensis	strain=DSM 28246	GCA_014212125.1	1387779	1387779	type	True	79.3223	514	1466	95	below_threshold
Paenibacillus albicereus	strain=UniB2	GCA_012676905.1	2726185	2726185	type	True	77.7457	326	1466	95	below_threshold
Paenibacillus artemisiicola	strain=MWE-103	GCA_017652985.1	1172618	1172618	type	True	77.5024	339	1466	95	below_threshold
Paenibacillus darwinianus	strain=Br	GCA_000598065.1	1380763	1380763	type	True	77.3829	152	1466	95	below_threshold
Paenibacillus methanolicus	strain=BL24	GCA_008124765.1	582686	582686	type	True	77.2205	284	1466	95	below_threshold
Cohnella herbarum	strain=MFER-1	GCA_012849095.1	2728023	2728023	type	True	77.0476	192	1466	95	below_threshold
Paenibacillus timonensis	strain=DSM 16943	GCA_022427145.1	225915	225915	type	True	76.9229	134	1466	95	below_threshold
Paenibacillus pinisoli	strain=JCM 19203	GCA_003605435.1	1276110	1276110	type	True	76.7271	158	1466	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:23:26,628] [INFO] DFAST Taxonomy check result was written to GCF_017874835.1_ASM1787483v1_genomic.fna/tc_result.tsv
[2024-01-24 13:23:26,629] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:23:26,629] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:23:26,629] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg7eac07df-6250-445b-bb99-ac462991293d/dqc_reference/checkm_data
[2024-01-24 13:23:26,630] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:23:26,670] [INFO] Task started: CheckM
[2024-01-24 13:23:26,670] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_017874835.1_ASM1787483v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_017874835.1_ASM1787483v1_genomic.fna/checkm_input GCF_017874835.1_ASM1787483v1_genomic.fna/checkm_result
[2024-01-24 13:24:03,980] [INFO] Task succeeded: CheckM
[2024-01-24 13:24:03,981] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:24:04,002] [INFO] ===== Completeness check finished =====
[2024-01-24 13:24:04,002] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:24:04,002] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_017874835.1_ASM1787483v1_genomic.fna/markers.fasta)
[2024-01-24 13:24:04,003] [INFO] Task started: Blastn
[2024-01-24 13:24:04,003] [INFO] Running command: blastn -query GCF_017874835.1_ASM1787483v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg7eac07df-6250-445b-bb99-ac462991293d/dqc_reference/reference_markers_gtdb.fasta -out GCF_017874835.1_ASM1787483v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:24:04,863] [INFO] Task succeeded: Blastn
[2024-01-24 13:24:04,866] [INFO] Selected 10 target genomes.
[2024-01-24 13:24:04,866] [INFO] Target genome list was writen to GCF_017874835.1_ASM1787483v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:24:04,881] [INFO] Task started: fastANI
[2024-01-24 13:24:04,882] [INFO] Running command: fastANI --query /var/lib/cwl/stgd560a7ee-10f9-4f27-aa55-ae150c2ec53d/GCF_017874835.1_ASM1787483v1_genomic.fna.gz --refList GCF_017874835.1_ASM1787483v1_genomic.fna/target_genomes_gtdb.txt --output GCF_017874835.1_ASM1787483v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:24:16,261] [INFO] Task succeeded: fastANI
[2024-01-24 13:24:16,273] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:24:16,273] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014212015.1	s__Cohnella lubricantis	99.9683	1403	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Cohnella	95.0	99.99	99.99	0.99	0.99	2	conclusive
GCF_014212175.1	s__Cohnella xylanilytica	81.1816	754	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Cohnella	95.0	98.90	98.90	0.93	0.93	2	-
GCF_000429825.1	s__Cohnella thermotolerans	80.7738	690	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Cohnella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014212055.1	s__Cohnella zeiphila	79.6938	594	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Cohnella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014212125.1	s__Cohnella nanjingensis	79.3316	513	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Cohnella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004801405.1	s__Cohnella sp004801405	79.3155	556	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Cohnella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003990975.1	s__Cohnella sp003990975	79.2924	512	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Cohnella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003208555.1	s__Cohnella sp003208555	78.6215	448	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Cohnella	95.0	99.99	99.99	1.00	1.00	2	-
GCF_008124765.1	s__Paenibacillus_Z methanolicus	77.2304	283	1466	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Paenibacillus_Z	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:24:16,275] [INFO] GTDB search result was written to GCF_017874835.1_ASM1787483v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:24:16,275] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:24:16,278] [INFO] DFAST_QC result json was written to GCF_017874835.1_ASM1787483v1_genomic.fna/dqc_result.json
[2024-01-24 13:24:16,279] [INFO] DFAST_QC completed!
[2024-01-24 13:24:16,279] [INFO] Total running time: 0h1m21s