[2024-01-24 10:57:26,713] [INFO] DFAST_QC pipeline started.
[2024-01-24 10:57:26,715] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 10:57:26,715] [INFO] DQC Reference Directory: /var/lib/cwl/stgaa2855ae-20e7-4862-ae92-d69b0aba0608/dqc_reference
[2024-01-24 10:57:27,989] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 10:57:27,990] [INFO] Task started: Prodigal
[2024-01-24 10:57:27,990] [INFO] Running command: gunzip -c /var/lib/cwl/stg61eeab3b-ee24-416c-b61b-32f98fd2aadb/GCF_018555385.1_ASM1855538v1_genomic.fna.gz | prodigal -d GCF_018555385.1_ASM1855538v1_genomic.fna/cds.fna -a GCF_018555385.1_ASM1855538v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 10:57:36,536] [INFO] Task succeeded: Prodigal
[2024-01-24 10:57:36,536] [INFO] Task started: HMMsearch
[2024-01-24 10:57:36,537] [INFO] Running command: hmmsearch --tblout GCF_018555385.1_ASM1855538v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgaa2855ae-20e7-4862-ae92-d69b0aba0608/dqc_reference/reference_markers.hmm GCF_018555385.1_ASM1855538v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 10:57:36,767] [INFO] Task succeeded: HMMsearch
[2024-01-24 10:57:36,769] [INFO] Found 6/6 markers.
[2024-01-24 10:57:36,794] [INFO] Query marker FASTA was written to GCF_018555385.1_ASM1855538v1_genomic.fna/markers.fasta
[2024-01-24 10:57:36,794] [INFO] Task started: Blastn
[2024-01-24 10:57:36,794] [INFO] Running command: blastn -query GCF_018555385.1_ASM1855538v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgaa2855ae-20e7-4862-ae92-d69b0aba0608/dqc_reference/reference_markers.fasta -out GCF_018555385.1_ASM1855538v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 10:57:37,918] [INFO] Task succeeded: Blastn
[2024-01-24 10:57:37,921] [INFO] Selected 13 target genomes.
[2024-01-24 10:57:37,922] [INFO] Target genome list was writen to GCF_018555385.1_ASM1855538v1_genomic.fna/target_genomes.txt
[2024-01-24 10:57:37,928] [INFO] Task started: fastANI
[2024-01-24 10:57:37,928] [INFO] Running command: fastANI --query /var/lib/cwl/stg61eeab3b-ee24-416c-b61b-32f98fd2aadb/GCF_018555385.1_ASM1855538v1_genomic.fna.gz --refList GCF_018555385.1_ASM1855538v1_genomic.fna/target_genomes.txt --output GCF_018555385.1_ASM1855538v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 10:57:46,673] [INFO] Task succeeded: fastANI
[2024-01-24 10:57:46,674] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgaa2855ae-20e7-4862-ae92-d69b0aba0608/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 10:57:46,674] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgaa2855ae-20e7-4862-ae92-d69b0aba0608/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 10:57:46,685] [INFO] Found 13 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 10:57:46,685] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 10:57:46,686] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Bifidobacterium santillanense	strain=MA2	GCA_018555385.1	2809028	2809028	type	True	100.0	909	910	95	conclusive
Bifidobacterium rousetti	strain=RST9	GCA_008698235.1	2045439	2045439	type	True	89.1632	670	910	95	below_threshold
Bifidobacterium callitrichos	strain=DSM 23973	GCA_000771665.1	762209	762209	type	True	88.7338	649	910	95	below_threshold
Bifidobacterium callitrichos	strain=DSM 23973	GCA_000741175.1	762209	762209	type	True	88.6408	672	910	95	below_threshold
Bifidobacterium saguinibicoloris	strain=79T10	GCA_019331725.1	2834433	2834433	type	True	87.5999	589	910	95	below_threshold
Bifidobacterium parmae	strain=Uis4E	GCA_002860365.1	361854	361854	type	True	87.5525	592	910	95	below_threshold
Bifidobacterium platyrrhinorum	strain=SMA15	GCA_010667645.1	2661628	2661628	type	True	87.285	578	910	95	below_threshold
Bifidobacterium stellenboschense	strain=DSM 23968	GCA_000741785.1	762211	762211	type	True	86.9178	583	910	95	below_threshold
Bifidobacterium amazonense	strain=MA1	GCA_018555435.2	2809027	2809027	type	True	84.7223	597	910	95	below_threshold
Bifidobacterium miconisargentati	strain=82T25	GCA_019331675.1	2834437	2834437	type	True	84.6615	561	910	95	below_threshold
Bifidobacterium pseudolongum subsp. pseudolongum	strain=ATCC 25526	GCA_022691205.1	31954	1694	type	True	79.7698	303	910	95	below_threshold
Bifidobacterium mizhiense	strain=S053-2	GCA_020884755.1	2879940	2879940	type	True	78.1002	179	910	95	below_threshold
Cellulomonas avistercoris	strain=Sa3CUA2	GCA_014836445.1	2762242	2762242	type	True	76.5101	182	910	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 10:57:46,687] [INFO] DFAST Taxonomy check result was written to GCF_018555385.1_ASM1855538v1_genomic.fna/tc_result.tsv
[2024-01-24 10:57:46,688] [INFO] ===== Taxonomy check completed =====
[2024-01-24 10:57:46,688] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 10:57:46,688] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgaa2855ae-20e7-4862-ae92-d69b0aba0608/dqc_reference/checkm_data
[2024-01-24 10:57:46,689] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 10:57:46,718] [INFO] Task started: CheckM
[2024-01-24 10:57:46,718] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_018555385.1_ASM1855538v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_018555385.1_ASM1855538v1_genomic.fna/checkm_input GCF_018555385.1_ASM1855538v1_genomic.fna/checkm_result
[2024-01-24 10:58:16,399] [INFO] Task succeeded: CheckM
[2024-01-24 10:58:16,401] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 10:58:16,425] [INFO] ===== Completeness check finished =====
[2024-01-24 10:58:16,425] [INFO] ===== Start GTDB Search =====
[2024-01-24 10:58:16,426] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_018555385.1_ASM1855538v1_genomic.fna/markers.fasta)
[2024-01-24 10:58:16,426] [INFO] Task started: Blastn
[2024-01-24 10:58:16,426] [INFO] Running command: blastn -query GCF_018555385.1_ASM1855538v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgaa2855ae-20e7-4862-ae92-d69b0aba0608/dqc_reference/reference_markers_gtdb.fasta -out GCF_018555385.1_ASM1855538v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 10:58:18,290] [INFO] Task succeeded: Blastn
[2024-01-24 10:58:18,293] [INFO] Selected 9 target genomes.
[2024-01-24 10:58:18,293] [INFO] Target genome list was writen to GCF_018555385.1_ASM1855538v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 10:58:18,299] [INFO] Task started: fastANI
[2024-01-24 10:58:18,300] [INFO] Running command: fastANI --query /var/lib/cwl/stg61eeab3b-ee24-416c-b61b-32f98fd2aadb/GCF_018555385.1_ASM1855538v1_genomic.fna.gz --refList GCF_018555385.1_ASM1855538v1_genomic.fna/target_genomes_gtdb.txt --output GCF_018555385.1_ASM1855538v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 10:58:24,770] [INFO] Task succeeded: fastANI
[2024-01-24 10:58:24,779] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 10:58:24,779] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_018555385.1	s__Bifidobacterium sp018555385	100.0	909	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_008698235.1	s__Bifidobacterium rousetti	89.1632	670	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	96.97	96.97	0.86	0.86	2	-
GCF_000741175.1	s__Bifidobacterium callitrichos	88.6408	672	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	97.40	96.52	0.91	0.88	6	-
GCF_018555635.1	s__Bifidobacterium sp018555635	88.0477	615	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002860365.1	s__Bifidobacterium parmae	87.5211	594	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_010667645.1	s__Bifidobacterium platyrrhinorum	87.2898	577	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000741785.1	s__Bifidobacterium stellenboschense	86.9512	582	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001417815.1	s__Bifidobacterium aesculapii	86.4667	554	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018555355.1	s__Bifidobacterium sp018555355	84.5159	538	910	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	96.26	96.26	0.85	0.85	2	-
--------------------------------------------------------------------------------
[2024-01-24 10:58:24,780] [INFO] GTDB search result was written to GCF_018555385.1_ASM1855538v1_genomic.fna/result_gtdb.tsv
[2024-01-24 10:58:24,781] [INFO] ===== GTDB Search completed =====
[2024-01-24 10:58:24,784] [INFO] DFAST_QC result json was written to GCF_018555385.1_ASM1855538v1_genomic.fna/dqc_result.json
[2024-01-24 10:58:24,784] [INFO] DFAST_QC completed!
[2024-01-24 10:58:24,784] [INFO] Total running time: 0h0m58s